| Gene: |
Gene Symbol:
GLE2 Gene Name: Nuclear pore protein required for poly(A)+ RNA export, has beta-transducin (WD-40) repeats Sequence Map: pDONR cofig.pdf |
|---|---|
| Original Clone ID: | FLH112827.01X |
| Keyword: | None |
| Species: | Saccharomyces cerevisiae |
| Type: | cDNA |
| Vector Name: | pDONR221 Format: CLOSED |
| Source: | HIP |
| Description: | None |
| Comments: | None |
| Mutation/ Discrepancy : |
No / No |
| Publications: | None |
| Authors: |
HIP |
|
ScCD00010188 Price: Login for Pricing AVAILABLE No restriction Special MTA: None |
Insert sequence: 1098nts
Open reading frame : 1 to 1098ATGTCTTTTTTTAATCGATCAAATACAACCTCAGCTTTGGGAACGAGTACTGCAATGGCG
AATGAAAAGGATTTGGCCAATGATATTGTCATTAATAGCCCAGCAGAGGACTCAATATCG
GATATTGCGTTTTCACCGCAACAAGATTTCATGTTTAGTGCCAGTTCCTGGGACGGTAAG
GTACGTATTTGGGATGTCCAAAATGGTGTACCTCAAGGTAGAGCGCAGCATGAAAGCTCC
AGCCCTGTATTATGTACCAGATGGTCGAATGATGGTACAAAAGTAGCCTCAGGCGGGTGT
GATAATGCCCTGAAATTGTACGATATTGCCAGTGGTCAAACACAGCAAATTGGAATGCAT
TCTGCGCCAATAAAAGTATTACGTTTTGTACAATGTGGACCATCGAACACAGAGTGTATA
GTAACCGGATCCTGGGATAAAACCATAAAATATTGGGATATGAGACAACCCCAGCCCGTA
TCCACCGTAATGATGCCTGAAAGAGTATATTCGATGGATAATAAACAGTCTCTGTTAGTA
GTGGCTACTGCCGAAAGACATATCGCTATTATCAATCTAGCAAACCCAACAACGATTTTT
AAGGCAACCACGTCACCTTTAAAATGGCAAACTCGATGCGTGGCATGTTATAATGAAGCC
GATGGATATGCGATTGGTTCAGTGGAAGGTAGATGCTCCATAAGGTATATCGATGATGGT
ATGCAGAAAAAATCAGGCTTTTCATTTAAATGCCATCGTCAAACCAATCCTAATAGGGCA
CCTGGCTCCAATGGTCAATCTCTAGTGTATCCTGTAAATAGCATTGCATTTCATCCATTA
TATGGTACTTTTGTTACGGCTGGTGGTGATGGTACCTTCAATTTTTGGGATAAGAATCAA
AGGCATAGATTGAAAGGCTATCCTACTTTGCAAGCATCTATTCCTGTATGCAGCTTCAAT
AGGAATGGTAGCGTATTTGCGTACGCTCTAAGTTATGATTGGCATCAAGGCCATATGGGC
AACAGACCCGACTATCCAAACGTCATTAGGCTACATGCCACAACTGATGAAGAGGTTAAA
GAGAAAAAGAAAAGGTGA
|
| Coding Sequence Details | |
|---|---|
| Insert Sequence Verified?: Y | Verification Method: Sequence Verification |
|
Reference Sequence Annotations: End on reference sequence 1098 Start on reference sequence 1 |
|
|
Mutations: None |
Discrepancy: None |
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi) |
|
3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC |
|
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
kanamycin Bacterial Selection Condition: 50ug/mL kanamycin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors. |
| Protein Expression Results: | None |
| Recommended expression in: | Not Applicable |
Powered by LabGenius |
||
| Vector Name: | pDONR221 |
pDONR221 in advanced viewed |
| Synonyms: | None | |
| Sequencing Primer: | Forward:
U19
Reverse: AttP2 |
|
| Description: | Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning. | |
| Comments: | The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website. | |
| Size (bp): | 4762 | |
| Parent Vector: | None | |
| Empty Vector: | None | |
| Properties: | Gateway, donor (entry), recombinational cloning | |
| Author Name: |
Invitrogen |
|
| Publications: | None | |
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| bacterial origin | ColE1 | ColE1 (pUC-type) origin of replication | 4086 | 4759 |
| negative selection marker | ccdB | ccdB negative selection gene (death cassette), LOST in recombined (with insert) form | 1502 | 1197 |
| primer site | M13 forward primer | M13 Forward priming site | 537 | 552 |
| primer site | M13 reverse primer | M13 Reverse priming site | 3027 | 3034 |
| recombination site | attP1 | attP recombination site 1 LOST in recombined (with insert) form | 570 | 801 |
| recombination site | attL1 | attL recombination site 1 present in recombined (with insert) form only, position is approximate | 570 | 801 |
| recombination site | attL2 | attL recombination site 2 present in recombined (with insert) form only, position is approximate | 2985 | 2754 |
| recombination site | attP2 | attP recombination site 2 LOST in recombined (with insert) form | 2985 | 2754 |
| selectable marker | CmR | chloramphenicol resistance gene, LOST in recombined (with insert) form | 2506 | 1847 |
| selectable marker | KanR | kanamycin resistance gene | 3156 | 3965 |
| trxn termination sequence | rrnB T2 | rrnB T2 transcription termination sequence | 295 | 268 |
| trxn termination sequence | rrnB T1 | rrnB T1 transcription termination sequence | 470 | 427 |
- Gene
- Protein
- Clone
- Organism
Protein
| SMART domain : | WD40 : WD40 repeats |
|---|
Clone
| Cloning Information : | 112827 |
|---|---|
| HIP Master Clone ID : | 88673 |
| Original Clone ID : | FLH112827.01X |
Organism
| Species Specific ID: | YER107C |
|---|
