DNASU Plasmid Repository • 480.965.5697 | Email

MUD2 (Saccharomyces cerevisiae) in ZM552 (V5-tagged yeast expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  MUD2
Gene Name:  Involved in early pre-mRNA splicing
Sequence             
Original Clone ID: FLH468889.01X
Keyword: None
Species: Saccharomyces cerevisiae
Type: cDNA
Vector Name: ZM552               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
ScCD00102078

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1584nts         Open reading frame : 1 to 1584

ATGGCTGATGAAAAGAGACTGGAGGATTTGAGATCCAAAATAATGGAGTCTATAGGAAAA
TCAGAAAAGGATGTTGTGCCGATTGAGAACAAAAGATTCAACACGGATAATGCAGTTATA
GATACACATTTTAAGAGGCAAAAAAGTGACGGTGAGCTTCCGAAAGCGCCAAAAAGTAGA
AACGTTTCACATTCTAATAACAGAGGACCATCGTCAATAATTACTATGAGCACGAATCGA
ACGACTTATGAACAAACAAGGGCAGGTCCTCATCGTCAAAGTTATCGGGATGCATCGGGA
AGATCATATAACAGGGAGAATCGGTATAGTAGTCACAATACTGGGCCCCAATGGAACAAC
AATCCCTATAATAGGCAACGGGATGAAAGAAGAGGAAGAAATGAAAGGTTCGATAGAAGG
GGAAGAAATGGAAACGGAAACTACGATCGTTTCAATTACCAAAGGAAGAACGAAGGTTCT
AAATTCAATGGCGACCGTGATAAAAGGCAGTTGCAAACTAATAAATATGACATGAACTAT
AATTCCCAAAATGTAATGTACCCGGGTTCATCATTCGATTCTCCGGCTTACTACAACATG
GCCTCATCCAAGGCAAATTCAAGGCTTGTAATTTCGGGCTTGTCTCAAAGCAGCGACCCC
AGCATTGTAGCAAGATTGAAAGATTTGCTGGAAAATTTTATTTCAGGTCTCCAGAAAACT
GAGAGTAATGCGGAGGACTTTAAAATTTCCAACTTTTATATTGGTGAAGGAAGGCCGGAC
CATATTATTGTTGAGTTTAGTTCTCAGATCTGTAGCACTATGGTTTTGGCCTGTCGATCA
TTCTTTAATGCGAAGCTTGGTACTTTCGACTTAAAATGGAGAAGACCTAATGATTATGTT
CAACAACTTGATCATCTGGTCGATTTTTGCAGGGGTACGGTAATTGCATTGGAAAATTTG
GAAAATATAGGCGAGGGTGAAGATTATAGAATGAAAGAGCTTTTTTCGTCATTGAATGTA
ACAAACGGTACCGCTAAGCCACTTTTTTACAAATGTTCGTCCAATACAAACAACACTGGC
AAAGAATCCGAATTCACTAAATGTATTCTATTATCGTTTGAAGTCGTCACTCAGGATATT
CTTGATAAATTAAAACCATACAAATGGTTCAAGCCAAATGATGGAAAGATATCACAAGTT
ACTTCATGGATTACGTTTCAAAGCCTGCCAAATCTGGTTACCCAATCTGTTCGTGTTGAA
TCGAGAGTTTTGTTGCTCTTAAATTGTCTTGATCCATTGGATTTGAAAGATGAAACCTTT
ATCACAGAAATTAAAGAAACATTAAAGTATAGTATCGCGGGAGCAGATACGATTAAAATA
TGCCAACCCGGCGTCGATTACAGACTTAATTTTGAAAACTTGGCTTCCGGAGCCGGTAAT
ATTTATATCAAGTTCAAGACCTTGGAGGCAGCAAAACACGCAATGGAAGAATTGCCGGGT
ACGCAATTCAACGATCGCACTGTTCTGTGTACTTATATAGATGAGGACGACTTTGACATG
ATGGAAGCAACCCAACTTTCCTAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1584
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  yeast    Marker:  LEU2
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: ZM552

ZM552 in advanced viewed

Synonyms: ZM552
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
Comments: ZM511 Stu1 vector plus Gateway attR frame B cassette EcoRV fragment; same as ZM515. Cloning into the vector removes the ccdB cassette and chloramphenicol resistance.
Size (bp): 8302
Parent Vector: None
Empty Vector: EvNO00335423
Properties: Gateway, acceptor (destination), recombinational cloning, with tag/fusion/marker, yeast expression
Author Name: Zarmik Moqtaderi
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
primer site M13R M13 reverse sequencing primer 2630 2647
primer site M13F M13 forward sequencing primer 8286 8301
promoter CMD1 CMD1 promoter 295 484
recombination site attR1 attR1 recombinational cloning site 650 666
recombination site attR2 attR2 recombination site 2337 2352
reporter gene LacZ LacZ alpha gene 8146 8214
selectable marker CmR chloramphenicol resistance. removed with the ccdB cassette in clones with insert 883 1539
selectable marker LEU2 LEU2 selectable marker in yeast 5273 5344
selectable marker AmpR ampcillin resistance 3795 4454
tag V5 N-terminal triple V5 tag 494 631
trxn termination sequence CYC1 CYC1 terminator 2377 2582
yeast ARS ARS ARS yeast origin of replication 0 0
yeast centromere CEN yeast centromere 0 0

 

  • Gene
  • Protein
  • Clone
  • Organism

Gene

Target GenBank: CAA81911

 

Protein

SMART domain : RRM_1 : RNA recognition motif

Clone

Cloning Information : 468889
HIP Master Clone ID : 176164
Original Clone ID : FLH468889.01X

Organism


Species Specific ID: YKL074C