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PSMD8 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  PSMD8
Gene Name:  proteasome (prosome, macropain) 26S subunit, non-ATPase, 8
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH009199.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00001022

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 774nts         Open reading frame : 1 to 774
Reference Sequence Alignment

ATGTACGAGCAACTCAAGGGCGAGTGGAACCGTAAAAGCCCCAATCTTAGCAAGTGCGGG
GAAGAGCTGGGTCGACTCAAGCTAGTTCTTCTGGAGCTCAACTTCTTGCCAACCACAGGG
ACCAAGCTGACCAAACAGCAGCTAATTCTGGCCCGTGACATACTGGAGATCGGGGCCCAA
TGGAGCATCCTACGCAAGGACATCCCCTCCTTCGAGCGCTACATGGCCCAGCTCAAATGC
TACTACTTTGATTACAAGGAGCAGCTCCCCGAGTCAGCCTATATGCACCAGCTCTTGGGC
CTCAACCTCCTCTTCCTGCTGTCCCAGAACCGGGTGGCTGAGTTCCACACGGAGTTGGAG
CGGCTGCCTGCCAAGGACATACAGACCAATGTCTACATCAAGCACCCAGTGTCCCTGGAG
CAATACCTGATGGAGGGCAGCTACAACAAAGTGTTCCTGGCCAAGGGTAACATCCCCGCC
GAGAGCTACACCTTCTTCATTGACATCCTGCTCGACACTATCAGGGATGAGATCGCTGGG
TGCATCGAGAAGGCCTACGAGAAAATCCTTTTCACTGAGGCCACCCGGATCCTCTTCTTC
AACACACCCAAAAAGATGACAGACTACGCCAAGAAGCGAGGGTGTGTCCTGGGCCCCAAC
AACTACTACAGTTTTGCCAGCCAGCAGCAGAAGCCGGAAGACACCACCATTCCCTCCACA
GAACTGGCCAAACAGGTCATCGAGTATGCCCGGCAGCTGGAGATGATCGTCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 844
Start on reference sequence 71
Mutations:
None
Discrepancy:
g645t,W215C
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PSMD8
Symbol Nomenclature : PSMD8
SYNONYM : HEL-S-91n; HIP6; HYPF; Nin1p; Rpn12; S14; p31
Designation : 26S proteasome non-ATPase regulatory subunit 8|26S proteasome regulatory subunit RPN12|26S proteasome regulatory subunit S14|26S proteasome regulatory subunit p31|epididymis secretory sperm binding protein Li 91n
Full Nomenclature : proteasome (prosome, macropain) 26S subunit, non-ATPase, 8
GENEID : 5714
GI : 61357817
GenBank Accession : AY888507
HGNC : 9566
Vega : OTTHUMG00000150691
Target GenBank: NM_002812

 

Reference Sequence Alignment

  

HsCD00001022      ------------------------------------------------------------
NM_002812.4       ATGTTCATTAAGGGCAGGGCTCCGAGGGCGCCACCTCGAGAGCGACGGCGGGCTACCCGG
                                                                              

HsCD00001022      ------------------------------------------------------------
NM_002812.4       GGCGGGCTGAGGCAGGTTGTAGCCCCGCCCCGGGCCTTGGGCTCCACCTCTCGGCCCCAC
                                                                              

HsCD00001022      ------------------------------------------------------------
NM_002812.4       TTCCGCCGGGCAAGCGTTTGTAGGCGGCGCTGCCGTAAATCAGGCGGTCTGCTTGCCGCA
                                                                              

HsCD00001022      ------------------------------------------------------------
NM_002812.4       TCACGCAAGATGGCGGCCGCGGCGGTGAACGGGGCGGCAGGCTTCTCGAGCTCCGGGCCC
                                                                              

HsCD00001022      ---------------------------------------ATGTACGAGCAACTCAAGGGC
NM_002812.4       GCGGCAACCTCGGGCGCTGTTCTGCAGGCCGCGACCGGCATGTACGAGCAACTCAAGGGC
                                                         *********************

HsCD00001022      GAGTGGAACCGTAAAAGCCCCAATCTTAGCAAGTGCGGGGAAGAGCTGGGTCGACTCAAG
NM_002812.4       GAGTGGAACCGTAAAAGCCCCAATCTTAGCAAGTGCGGGGAAGAGCTGGGTCGACTCAAG
                  ************************************************************

HsCD00001022      CTAGTTCTTCTGGAGCTCAACTTCTTGCCAACCACAGGGACCAAGCTGACCAAACAGCAG
NM_002812.4       CTAGTTCTTCTGGAGCTCAACTTCTTGCCAACCACAGGGACCAAGCTGACCAAACAGCAG
                  ************************************************************

HsCD00001022      CTAATTCTGGCCCGTGACATACTGGAGATCGGGGCCCAATGGAGCATCCTACGCAAGGAC
NM_002812.4       CTAATTCTGGCCCGTGACATACTGGAGATCGGGGCCCAATGGAGCATCCTACGCAAGGAC
                  ************************************************************

HsCD00001022      ATCCCCTCCTTCGAGCGCTACATGGCCCAGCTCAAATGCTACTACTTTGATTACAAGGAG
NM_002812.4       ATCCCCTCCTTCGAGCGCTACATGGCCCAGCTCAAATGCTACTACTTTGATTACAAGGAG
                  ************************************************************

HsCD00001022      CAGCTCCCCGAGTCAGCCTATATGCACCAGCTCTTGGGCCTCAACCTCCTCTTCCTGCTG
NM_002812.4       CAGCTCCCCGAGTCAGCCTATATGCACCAGCTCTTGGGCCTCAACCTCCTCTTCCTGCTG
                  ************************************************************

HsCD00001022      TCCCAGAACCGGGTGGCTGAGTTCCACACGGAGTTGGAGCGGCTGCCTGCCAAGGACATA
NM_002812.4       TCCCAGAACCGGGTGGCTGAGTTCCACACGGAGTTGGAGCGGCTGCCTGCCAAGGACATA
                  ************************************************************

HsCD00001022      CAGACCAATGTCTACATCAAGCACCCAGTGTCCCTGGAGCAATACCTGATGGAGGGCAGC
NM_002812.4       CAGACCAATGTCTACATCAAGCACCCAGTGTCCCTGGAGCAATACCTGATGGAGGGCAGC
                  ************************************************************

HsCD00001022      TACAACAAAGTGTTCCTGGCCAAGGGTAACATCCCCGCCGAGAGCTACACCTTCTTCATT
NM_002812.4       TACAACAAAGTGTTCCTGGCCAAGGGTAACATCCCCGCCGAGAGCTACACCTTCTTCATT
                  ************************************************************

HsCD00001022      GACATCCTGCTCGACACTATCAGGGATGAGATCGCTGGGTGCATCGAGAAGGCCTACGAG
NM_002812.4       GACATCCTGCTCGACACTATCAGGGATGAGATCGCTGGGTGCATCGAGAAGGCCTACGAG
                  ************************************************************

HsCD00001022      AAAATCCTTTTCACTGAGGCCACCCGGATCCTCTTCTTCAACACACCCAAAAAGATGACA
NM_002812.4       AAAATCCTTTTCACTGAGGCCACCCGGATCCTCTTCTTCAACACACCCAAAAAGATGACA
                  ************************************************************

HsCD00001022      GACTACGCCAAGAAGCGAGGGTGTGTCCTGGGCCCCAACAACTACTACAGTTTTGCCAGC
NM_002812.4       GACTACGCCAAGAAGCGAGGGTGGGTCCTGGGCCCCAACAACTACTACAGTTTTGCCAGC
                  *********************** ************************************

HsCD00001022      CAGCAGCAGAAGCCGGAAGACACCACCATTCCCTCCACAGAACTGGCCAAACAGGTCATC
NM_002812.4       CAGCAGCAGAAGCCGGAAGACACCACCATTCCCTCCACAGAACTGGCCAAACAGGTCATC
                  ************************************************************

HsCD00001022      GAGTATGCCCGGCAGCTGGAGATGATCGTCTAG
NM_002812.4       GAGTATGCCCGGCAGCTGGAGATGATCGTCTGA
                  *******************************..

Pathways

Reactome : APC/C-mediated degradation of cell cycle proteins
Reactome : APC/C:Cdc20 mediated degradation of Securin
Reactome : APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome : APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
Reactome : AUF1 (hnRNP D0) destabilizes mRNA
Reactome : Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome : Activation of NF-kappaB in B cells
Reactome : Adaptive Immune System
Reactome : Antigen processing-Cross presentation
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Apoptosis
Reactome : Assembly of the pre-replicative complex
Reactome : Asymmetric localization of PCP proteins
Reactome : Autodegradation of Cdh1 by Cdh1:APC/C
Reactome : Autodegradation of the E3 ubiquitin ligase COP1
Reactome : CDK-mediated phosphorylation and removal of Cdc6
Reactome : CDT1 association with the CDC6:ORC:origin complex
Reactome : Cdc20:Phospho-APC/C mediated degradation of Cyclin A
Reactome : Cell Cycle
Reactome : Cell Cycle Checkpoints
Reactome : Cell Cycle, Mitotic
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Cross-presentation of soluble exogenous antigens (endosomes)
Reactome : Cyclin A:Cdk2-associated events at S phase entry
Reactome : Cyclin E associated events during G1/S transition
Reactome : DNA Replication
Reactome : DNA Replication Pre-Initiation
Reactome : Disease
Reactome : Downstream signaling events of B Cell Receptor (BCR)
Reactome : ER-Phagosome pathway
Reactome : G1/S DNA Damage Checkpoints
Reactome : G1/S Transition
Reactome : Gene Expression
Reactome : HIV Infection
Reactome : Host Interactions of HIV factors
Reactome : Immune System
Reactome : M Phase
Reactome : M/G1 Transition
Reactome : Metabolism
Reactome : Metabolism of RNA
Reactome : Metabolism of amino acids and derivatives
Reactome : Metabolism of mRNA
Reactome : Mitotic Anaphase
Reactome : Mitotic G1-G1/S phases
Reactome : Mitotic M-M/G1 phases
Reactome : Mitotic Metaphase and Anaphase
Reactome : Orc1 removal from chromatin
Reactome : PCP/CE pathway
Reactome : Regulation of APC/C activators between G1/S and early anaphase
Reactome : Regulation of Apoptosis
Reactome : Regulation of DNA replication
Reactome : Regulation of activated PAK-2p34 by proteasome mediated degradation
Reactome : Regulation of mRNA stability by proteins that bind AU-rich elements
Reactome : Regulation of mitotic cell cycle
Reactome : Regulation of ornithine decarboxylase (ODC)
Reactome : Removal of licensing factors from origins
Reactome : S Phase
Reactome : SCF(Skp2)-mediated degradation of p27/p21
Reactome : SCF-beta-TrCP mediated degradation of Emi1
Reactome : Separation of Sister Chromatids
Reactome : Signal Transduction
Reactome : Signaling by Wnt
Reactome : Signaling by the B Cell Receptor (BCR)
Reactome : Stabilization of p53
Reactome : Switching of origins to a post-replicative state
Reactome : Synthesis of DNA
Reactome : TCF dependent signaling in response to WNT
Reactome : Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
Reactome : Ubiquitin-dependent degradation of Cyclin D
Reactome : Ubiquitin-dependent degradation of Cyclin D1
Reactome : Vif-mediated degradation of APOBEC3G
Reactome : Vpu mediated degradation of CD4
Reactome : beta-catenin independent WNT signaling
Reactome : degradation of AXIN
Reactome : degradation of DVL
Reactome : p53-Dependent G1 DNA Damage Response
Reactome : p53-Dependent G1/S DNA damage checkpoint
Reactome : p53-Independent DNA Damage Response
Reactome : p53-Independent G1/S DNA damage checkpoint
WikiPathway : Proteasome Degradation

Protein

UniProt : P48556
HPRD : 10171

Clone

Cloning Information : 9199
HIP Master Clone ID : 11767
Original Clone ID : FLH009199.01X

Organism


TAX_ID : 9606
Species Specific ID: 5714

Genome

Chromosome : 19
Map Location : 19q13.2
Ensembl : ENSG00000099341

Reagents

Labome : PSMD8-antibody