Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name:  None
Original Clone ID:
35675
Keyword:
None
Species:
Shewanella amazonensis SB2B
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 19000695
Title: A structural basis for the regulatory inactivation of DnaA.
Authors:
PSI
Joint Center for Structural Genomics
SaCD00103804
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 726nts
Open reading frame: 51 to 776

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAGAAGTA
ACCGAGTGACACAACATCCCCCATTGCAGTTGTCCCTGCCTGTACATTTGCCAGACGATG
AGACCTTTACCAGTTATTACCCCGCGGCGGGTAACGACGAGCTTATCGGTGCGCTGAAAT
CGGCGGCAAGTGGCGATGGGGTGCAGGCTATTTATTTGTGGGGACCGGTCAAGTCGGGAC
GCACCCATCTTATCCATGCGGCCTGCGCCCGTGCCAATGAGCTGGAGCGCCGCAGTTTCT
ATATTCCGCTCGGAATTCACGCCAGTATTTCCACGGCCTTGTTGGAAGGGTTGGAGCAGT
TTGACCTTATCTGTATCGATGATGTAGATGCGGTGGCTGGTCACCCCCTGTGGGAAGAGG
CGATATTTGATCTCTACAACCGGGTAGCAGAGCAAAAACGTGGCAGTTTGATAGTCAGTG
CCAGTGCTTCGCCCATGGAGGCGGGTTTTGTGCTGCCGGATCTCGTGTCCAGAATGCATT
GGGGGCTGACTTACCAGCTCCAGCCTATGATGGACGATGAGAAACTCGCGGCATTGCAAC
GCCGTGCTGCCATGCGCGGACTCCAGTTACCTGAAGACGTGGGGCGGTTCTTACTTAATC
GCATGGCGCGAGATTTACGCACACTGTTTGATGTCCTCGACCGGCTTGATAAGGCGTCCA
TGGTTCATCAGCGCAAGCTGACCATACCTTTTGTCAAAGAGATGCTGAGGCTGTAACGCG
ACTTAATTAA

Protein sequence predicted based on nucleotide sequence

MRSNRVTQHPPLQLSLPVHLPDDETFTSYYPAAGNDELIGALKSAASGDGVQAIYLWGPV
KSGRTHLIHAACARANELERRSFYIPLGIHASISTALLEGLEQFDLICIDDVDAVAGHPL
WEEAIFDLYNRVAEQKRGSLIVSASASPMEAGFVLPDLVSRMHWGLTYQLQPMMDDEKLA
ALQRRAAMRGLQLPEDVGRFLLNRMARDLRTLFDVLDRLDKASMVHQRKLTIPFVKEMLR
L*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 726
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 378832
Recommended expression in:
Not Applicable