Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
40660
Keyword:
None
Species:
Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 22467785
Title: The structure of Mlc titration factor A (MtfA/Yeel) reveals a prototypical zinc metalloptidase related to anthrax lethal factor.
Authors:
PSI
Joint Center for Structural Genomics
KpCD00104687
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 801nts
Open reading frame: 51 to 851

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGATGTTTA
AATGGCCCTGGAAAGCAGATGATGAGTCCGGCAACGCAGAGATGCCCTGGGAGCAAGCGC
TTGCCATTCCGGTTTTAGCCCATCTTTCGTCCACTGAGCAGCACAAGCTCACGCAGATGG
CTGCCCGTTTTTTACAGCAAAAGCGGCTGGTGGCATTGCAGGGGCTGGAGTTGACCCCGC
TGCATCAGGCGCGCATTGCTATGCTCTTTTGTCTGCCGGTGCTTGAGCTGGGCATTGAGT
GGCTGGATGGCTTCCATGAAGTGCTGATCTACCCTGCCCCTTTCATCGTCGATGATGAAT
GGGAGGATGATATTGGTCTGGTCCACAACCAGCGGGTGGTACAGTCGGGACAAAGCTGGC
AACAGGGTCCTGTAGTGCTGAACTGGCTGGATATTCAAGACTCTTTTGATGCGTCAGGCT
TTAACCTTGTGGTGCATGAAGTGGCGCATAAGCTTGATACGCGGAACGGCGACCGGGCCA
GCGGCGTGCCGCTTATCCCGTTGCGTGAAGTTGCCGGCTGGGAGCATGATCTCCACGCCG
CGATGAACAACATTCAGGATGAGATCGATCTGGTCGGCGAAAGCGCCGCCAGTATTGACG
CCTATGCCGCCACCGATCCCGCAGAGTGCTTTGCTGTCCTCTCGGAATATTTTTTCAGTG
CGCCTGAACTGTTCGCGCCTCGCTTCCCGGCCCTGTGGCAGCGTTTTTGCCACTTTTACC
GCCAGGATCCGCTGGCGCGTAGACGTGAGAATGGCCTGCAGGACGAAGGCGATCGGCGCA
TTGTTCACTAACGCGACTTAATTAATTTGGCGGATGAGAGAAGATTTTCAGCCTGATACA

Protein sequence predicted based on nucleotide sequence

MMFKWPWKADDESGNAEMPWEQALAIPVLAHLSSTEQHKLTQMAARFLQQKRLVALQGLE
LTPLHQARIAMLFCLPVLELGIEWLDGFHEVLIYPAPFIVDDEWEDDIGLVHNQRVVQSG
QSWQQGPVVLNWLDIQDSFDASGFNLVVHEVAHKLDTRNGDRASGVPLIPLREVAGWEHD
LHAAMNNIQDEIDLVGESAASIDAYAATDPAECFAVLSEYFFSAPELFAPRFPALWQRFC
HFYRQDPLARRRENGLQDEGDRRIVH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 801
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 388889
Recommended expression in:
Not Applicable