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AQP9 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  AQP9
Gene Name:  aquaporin 9
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH131276.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00001134

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 888nts         Open reading frame : 1 to 888
Reference Sequence Alignment

ATGCAGCCTGAGGGAGCAGAAAAGGGAAAAAGCTTCAAGCAGAGACTGGTCTTGAAGAGC
AGCTTAGCGAAAGAAACCCTCTCTGAGTTCTTGGGCACGTTCATCTTGATTGTCCTTGGA
TGTGGCTGTGTTGCCCAAGCTATTCTCAGTCGAGGACGTTTTGGAGGGGTCATCACTATC
AATGTTGGATTTTCAATGGCAGTTGCAATGGCCATTTATGTGGCTGGCGGTGTCTCTGGT
GGTCACATCAACCCAGCTGTGTCTTTAGCAATGTGTCTCTTTGGACGGATGAAATGGTTC
AAATTGCCATTTTATGTGGGAGCCCAGTTCTTGGGAGCCTTTGTGGGGGCTGCAACCGTC
TTTGGCATTTACTATGATGGACTTATGTCCTTTGCTGGTGGAAAACTGCTGATCGTGGGA
GAAAATGCAACAGCACACATTTTTGCAACATACCCAGCTCCGTATCTATCTCTGGCGAAC
GCATTTGCAGATCAAGTGGTGGCCACCATGATACTCCTCATAATCGTCTTTGCCATTTTT
GACTCCAGAAACTTGGGAGCCCCCAGAGGCCTAGAGCCCATTGCCATCGGCCTCCTGATT
ATTGTCATTGCTTCCTCCCTGGGACTGAACAGTGGCTGTGCCATGAACCCAGCTCGAGAC
CTGAGTCCCAGACTTTTCACTGCCTTGGCAGGCTGGGGGTTTGAAGTCTTCAGAGCTGGA
AACAACTTCTGGTGGATTCCTGTAGTGGGCCCTTTGGTTGGTGCTGTCATTGGAGGCCTC
ATCTATGTTCTTGTCATTGAAATCCACCATCCAGAGCCTGACTCAGTCTTTAAGGCAGAA
CAATCTGAGGACAAACCAGAGAAATATGAACTCAGTGTCATCATGGAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1174
Start on reference sequence 287
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
(goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : AQP9
Symbol Nomenclature : AQP9
SYNONYM : AQP-9; HsT17287; SSC1
Designation : aquaglyceroporin-9|aquaporin-9|small solute channel 1
Full Nomenclature : aquaporin 9
GENEID : 366
GI : 60827122
GenBank Accession : AY893748
HGNC : 643
MIM : 602914
Vega : OTTHUMG00000132631
Target GenBank: NM_020980

 

Reference Sequence Alignment

  

HsCD00001134      ATGCAGCCTGAGGGAGCAGAAAAGGGAAAAAGCTTCAAGCAGAGACTGGTCTTGAAGAGC
NM_020980.3       ATGCAGCCTGAGGGAGCAGAAAAGGGAAAAAGCTTCAAGCAGAGACTGGTCTTGAAGAGC
                  ************************************************************

HsCD00001134      AGCTTAGCGAAAGAAACCCTCTCTGAGTTCTTGGGCACGTTCATCTTGATTGTCCTTGGA
NM_020980.3       AGCTTAGCGAAAGAAACCCTCTCTGAGTTCTTGGGCACGTTCATCTTGATTGTCCTTGGA
                  ************************************************************

HsCD00001134      TGTGGCTGTGTTGCCCAAGCTATTCTCAGTCGAGGACGTTTTGGAGGGGTCATCACTATC
NM_020980.3       TGTGGCTGTGTTGCCCAAGCTATTCTCAGTCGAGGACGTTTTGGAGGGGTCATCACTATC
                  ************************************************************

HsCD00001134      AATGTTGGATTTTCAATGGCAGTTGCAATGGCCATTTATGTGGCTGGCGGTGTCTCTGGT
NM_020980.3       AATGTTGGATTTTCAATGGCAGTTGCAATGGCCATTTATGTGGCTGGCGGTGTCTCTGGT
                  ************************************************************

HsCD00001134      GGTCACATCAACCCAGCTGTGTCTTTAGCAATGTGTCTCTTTGGACGGATGAAATGGTTC
NM_020980.3       GGTCACATCAACCCAGCTGTGTCTTTAGCAATGTGTCTCTTTGGACGGATGAAATGGTTC
                  ************************************************************

HsCD00001134      AAATTGCCATTTTATGTGGGAGCCCAGTTCTTGGGAGCCTTTGTGGGGGCTGCAACCGTC
NM_020980.3       AAATTGCCATTTTATGTGGGAGCCCAGTTCTTGGGAGCCTTTGTGGGGGCTGCAACCGTC
                  ************************************************************

HsCD00001134      TTTGGCATTTACTATGATGGACTTATGTCCTTTGCTGGTGGAAAACTGCTGATCGTGGGA
NM_020980.3       TTTGGCATTTACTATGATGGACTTATGTCCTTTGCTGGTGGAAAACTGCTGATCGTGGGA
                  ************************************************************

HsCD00001134      GAAAATGCAACAGCACACATTTTTGCAACATACCCAGCTCCGTATCTATCTCTGGCGAAC
NM_020980.3       GAAAATGCAACAGCACACATTTTTGCAACATACCCAGCTCCGTATCTATCTCTGGCGAAC
                  ************************************************************

HsCD00001134      GCATTTGCAGATCAAGTGGTGGCCACCATGATACTCCTCATAATCGTCTTTGCCATTTTT
NM_020980.3       GCATTTGCAGATCAAGTGGTGGCCACCATGATACTCCTCATAATCGTCTTTGCCATCTTT
                  ******************************************************** ***

HsCD00001134      GACTCCAGAAACTTGGGAGCCCCCAGAGGCCTAGAGCCCATTGCCATCGGCCTCCTGATT
NM_020980.3       GACTCCAGAAACTTGGGAGCCCCCAGAGGCCTAGAGCCCATTGCCATCGGCCTCCTGATT
                  ************************************************************

HsCD00001134      ATTGTCATTGCTTCCTCCCTGGGACTGAACAGTGGCTGTGCCATGAACCCAGCTCGAGAC
NM_020980.3       ATTGTCATTGCTTCCTCCCTGGGACTGAACAGTGGCTGTGCCATGAACCCAGCTCGAGAC
                  ************************************************************

HsCD00001134      CTGAGTCCCAGACTTTTCACTGCCTTGGCAGGCTGGGGGTTTGAAGTCTTCAGAGCTGGA
NM_020980.3       CTGAGTCCCAGACTTTTCACTGCCTTGGCAGGCTGGGGGTTTGAAGTCTTCAGAGCTGGA
                  ************************************************************

HsCD00001134      AACAACTTCTGGTGGATTCCTGTAGTGGGCCCTTTGGTTGGTGCTGTCATTGGAGGCCTC
NM_020980.3       AACAACTTCTGGTGGATTCCTGTAGTGGGCCCTTTGGTTGGTGCTGTCATTGGAGGCCTC
                  ************************************************************

HsCD00001134      ATCTATGTTCTTGTCATTGAAATCCACCATCCAGAGCCTGACTCAGTCTTTAAGGCAGAA
NM_020980.3       ATCTATGTTCTTGTCATTGAAATCCACCATCCAGAGCCTGACTCAGTCTTTAAGACAGAA
                  ******************************************************.*****

HsCD00001134      CAATCTGAGGACAAACCAGAGAAATATGAACTCAGTGTCATCATGGAC
NM_020980.3       CAATCTGAGGACAAACCAGAGAAATATGAACTCAGTGTCATCATGTAG
                  ********************************************* * 

Pathways

Reactome : Aquaporin-mediated transport
Reactome : Passive transport by Aquaporins
Reactome : Transmembrane transport of small molecules
Reactome : Transport of glycerol from adipocytes to the liver by Aquaporins
WikiPathway : Integrated Breast Cancer Pathway

Protein

UniProt : O14520
HPRD : 04223

Clone

Cloning Information : 131276
HIP Master Clone ID : 107433
Original Clone ID : FLH131276.01L

Organism


TAX_ID : 9606
Species Specific ID: 364

Genome

Chromosome : 15
Map Location : 15q
Ensembl : ENSG00000103569

Reagents

Labome : AQP9-antibody