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MOT3 (Saccharomyces cerevisiae) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  MOT3
Gene Name:  DNA-binding protein implicated in heme-dependent repression, repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and regulation of membrane-related genes
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH158370.01X
Keyword: None
Species: Saccharomyces cerevisiae
Type: cDNA
Vector Name: pDONR201               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
ScCD00011588

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1473nts         Open reading frame : 1 to 1473

ATGAATGCGGACCATCACCTGCAACAGCAGCAGCAACAGCGACAACAGCATCAACAACAA
CAGCATCAACAACAACAGCATCAGCATCAGCATCAACAGCAGCAGCACACGATATTGCAA
AATGTGTCGAACACTAACAATATCGGCAGCGATTCGCTGGCGTCACAGCCTTTCAACACG
ACTACTGTTTCCTCTAACAAGGACGACGTTATGGTGAACTCTGGGGCAAGAGAACTTCCA
ATGCCCTTACATCAGCAGCAGTATATATACCCTTACTATCAGTATACAAGTAATAACAGT
AACAACAATAATGTGACGGCTGGTAACAATATGTCTGCGTCGCCGATTGTCCATAACAAC
AGCAACAACAGCAACAACAGCAATATTTCTGCTTCTGATTACACTGTCGCAAACAACAGT
ACTAGCAATAATAACAATAATAATAATAATAACAACAATAATAACAATAATATTCACCCA
AACCAGTTTACTGCGGCCGCAAATATGAACTCAAATGCTGCAGCGGCTGCTTATTACTCC
TTCCCCACTGCGAATATGCCAATACCGCAACAGGATCAACAATATATGTTCAATCCTGCT
TCATACATAAGCCATTACTATTCAGCAGTTAACAGCAATAACAATGGTAATAACGCCGCT
AACAATGGCAGCAACAACTCTTCTCACTCAGCCCCAGCCCCGGCCCCCGGTCCACCCCAT
CACCATCACCATCATAGTAATACACACAACAACCTCAACAATGGTGGTGCTGTAAATACA
AACAACGCTCCTCAGCACCATCCAACGATAATAACGGATCAATTTCAATTCCAACTACAA
CAAAACCCTTCTCCAAATTTGAATCTCAATATTAACCCGGCACAACCTCTGCATCTACCT
CCTGGTTGGAAAATAAACACTATGCCGCAACCACGTCCTACGACAGCACCTAACCATCCC
CCTGCGCCGGTGCCTTCTTCGAACCCTGTGGCCTCGAACTTGGTTCCTGCCCCATCATCA
GACCATAAATATATCCATCAATGCCAATTTTGTGAGAAGTCTTTCAAAAGAAAATCATGG
TTGAAAAGGCACCTATTGTCACACTCGCAACAAAGACATTTTCTATGCCCTTGGTGCTTA
AGCAGGCAGAAGAGAAAAGATAATCTTTTACAGCATATGAAACTCAAGCATACAAATTAT
TTATTAGACGAACTCAAGAAAAACAACATCATCTTTAACTACAACAATTCTTCCTCCTCT
AATAATAACAACGACAATAATAATAATAATAACAGCAATAGCGCTAGCGGCAGTGGCGGT
GCCGGTGCCGCGGCGGCAGCAGCAACAGCTCCCGAAAATGAAGATGGAAACGGTTACGAT
ACAAACATCAAGACTTTAATCAATGATGGTGTACTGAATAAGGACGACGTTAAACGTGTT
TTGAATAACCTTATTGTTAGTCACAACAAATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1473
Start on reference sequence 1
Mutations:
None
Discrepancy:
a117g
                                

5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Protein
  • Clone
  • Organism

Gene

Target GenBank: CAA88795

 

Protein

SMART domain : ZnF_C2H2 : zinc finger

Clone

Cloning Information : 158370
HIP Master Clone ID : 90005
Original Clone ID : FLH158370.01X

Organism


Species Specific ID: YMR070W