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POLB (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  POLB
Gene Name:  polymerase (DNA directed), beta
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH130961.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00001271

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1008nts         Open reading frame : 1 to 1008
Reference Sequence Alignment

ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
ACACTAGAAGATCTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTG
AAATATTTTGGGGACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGAT
ATTGTACTAAATGAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGT
TTCAGAAGAGGTGCAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTC
ACTTCAGAATCAACCAAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGTTACAAAAG
GTTCATTTTATCACAGATACCCTGTCAAAGGGTGAGACAAAGTTCATGGGTGTTTGCCAG
CTTCCCAGTAAAAATGATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATA
CCCAAAGATCAGTATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAG
AATATGAGGGCTCATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCC
TTGGGAGTCACTGGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTT
GATTACATCCAGTGGAAATACCGGGAACCCAAGGACCGGAGCGAAGAC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1121
Start on reference sequence 114
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
(goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : POLB
Symbol Nomenclature : POLB
Designation : DNA pol beta|DNA polymerase beta|DNA polymerase beta subunit|mutant DNA polymerase beta
Full Nomenclature : polymerase (DNA directed), beta
GENEID : 5423
GI : 60827765
GenBank Accession : AY893775
HGNC : 9174
MIM : 174760
Vega : OTTHUMG00000164093
Target GenBank: NM_002690

 

Reference Sequence Alignment

  

HsCD00001271        ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
XM_005273537.1      ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
XM_005273535.1      ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
NM_002690.2         ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACA
                    ************************************************************

HsCD00001271        GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
XM_005273537.1      GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
XM_005273535.1      GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
NM_002690.2         GAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGA
                    ************************************************************

HsCD00001271        AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
XM_005273537.1      AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
XM_005273535.1      AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
NM_002690.2         AAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAG
                    ************************************************************

HsCD00001271        AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
XM_005273537.1      AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
XM_005273535.1      AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
NM_002690.2         AAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGTTTTTAGCAACTGGA
                    ************************************************************

HsCD00001271        AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
XM_005273537.1      AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
XM_005273535.1      AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
NM_002690.2         AAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCATCCATCAATTTCCTG
                    ************************************************************

HsCD00001271        ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
XM_005273537.1      ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
XM_005273535.1      ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
NM_002690.2         ACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGAAGGAATTAAA
                    ************************************************************

HsCD00001271        ACACTAGAAG--------------------------------------------------
XM_005273537.1      ACACTAG---AAG-----------------------------------------------
XM_005273535.1      ACACTAG---AAGGCTCACAGCTGGATTCATGCCCAGTAAAGGGACACCTGAATGGAACT
NM_002690.2         ACACTAG---AAG-----------------------------------------------
                    *******                                                     

HsCD00001271        ----------------------------------------------------------AT
XM_005273537.1      ----------------------------------------------------------AT
XM_005273535.1      GAGTCACTTTTAGACTTAATATGGGATGTTATGACAATTCTTAAGTTAAAAAATGCAGAT
NM_002690.2         ----------------------------------------------------------AT
                                                                              **

HsCD00001271        CTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTGAAATATTTTGGG
XM_005273537.1      CTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTGAAATATTTTGGG
XM_005273535.1      CTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTGAAATATTTTGGG
NM_002690.2         CTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTGAAATATTTTGGG
                    ************************************************************

HsCD00001271        GACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAAAT
XM_005273537.1      GACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAAAT
XM_005273535.1      GACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAAAT
NM_002690.2         GACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAAAT
                    ************************************************************

HsCD00001271        GAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGT
XM_005273537.1      GAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGT
XM_005273535.1      GAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGT
NM_002690.2         GAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGT
                    ************************************************************

HsCD00001271        GCAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTCACTTCAGAATCA
XM_005273537.1      GCAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTCACTTCAGAATCA
XM_005273535.1      GCAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTCACTTCAGAATCA
NM_002690.2         GCAGAGTCCAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTCACTTCAGAATCA
                    ************************************************************

HsCD00001271        ACCAAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGTTACAAAAGGTTCATTTTATC
XM_005273537.1      ACCAAACAG---------------------------------------------------
XM_005273535.1      ACCAAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGTTACAAAAGGTTCATTTTATC
NM_002690.2         ACCAAACAGCCAAAACTGTTACATCAGGTTGTGGAGCAGTTACAAAAGGTTCATTTTATC
                    *********                                                   

HsCD00001271        ACAGATACCCTGTCAAAGGGTGAGACAAAGTTCATGGGTGTTTGCCAGCTTCCCAGTAAA
XM_005273537.1      ------------------------------------GGTGTTTGCCAGCTTCCCAGTAAA
XM_005273535.1      ACAGATACCCTGTCAAAGGGTGAGACAAAGTTCATGGGTGTTTGCCAGCTTCCCAGTAAA
NM_002690.2         ACAGATACCCTGTCAAAGGGTGAGACAAAGTTCATGGGTGTTTGCCAGCTTCCCAGTAAA
                                                        ************************

HsCD00001271        AATGATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATACCCAAAGATCAG
XM_005273537.1      AATGATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATACCCAAAGATCAG
XM_005273535.1      AATGATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATACCCAAAGATCAG
NM_002690.2         AATGATGAAAAAGAATATCCACACAGAAGAATTGATATCAGGTTGATACCCAAAGATCAG
                    ************************************************************

HsCD00001271        TATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAGAATATGAGGGCT
XM_005273537.1      TATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAGAATATGAGGGCT
XM_005273535.1      TATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAGAATATGAGGGCT
NM_002690.2         TATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAGAATATGAGGGCT
                    ************************************************************

HsCD00001271        CATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCCTTGGGAGTCACT
XM_005273537.1      CATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCCTTGGGAGTCACT
XM_005273535.1      CATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCCTTGGGAGTCACT
NM_002690.2         CATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCCTTGGGAGTCACT
                    ************************************************************

HsCD00001271        GGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTTGATTACATCCAG
XM_005273537.1      GGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTTGATTACATCCAG
XM_005273535.1      GGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTTGATTACATCCAG
NM_002690.2         GGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTTGATTACATCCAG
                    ************************************************************

HsCD00001271        TGGAAATACCGGGAACCCAAGGACCGGAGCGAAGAC
XM_005273537.1      TGGAAATACCGGGAACCCAAGGACCGGAGCGAATGA
XM_005273535.1      TGGAAATACCGGGAACCCAAGGACCGGAGCGAATGA
NM_002690.2         TGGAAATACCGGGAACCCAAGGACCGGAGCGAATGA
                    ********************************* ..

Pathways

Reactome : Base Excision Repair
Reactome : Base-free sugar-phosphate removal via the single-nucleotide replacement pathway
Reactome : DNA Repair
Reactome : Removal of DNA patch containing abasic residue
Reactome : Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Reactome : Resolution of AP sites via the single-nucleotide replacement pathway
Reactome : Resolution of Abasic Sites (AP sites)
WikiPathway : Nucleotide Metabolism

Protein

SMART domain : POLXc : DNA polymerase X family
UniProt : P06746
HPRD : 07517

Clone

Cloning Information : 130961
HIP Master Clone ID : 107118
Original Clone ID : FLH130961.01L

Organism


TAX_ID : 9606
Species Specific ID: 5423

Genome

Chromosome : 8
Map Location : 8p11.2
Ensembl : ENSG00000070501