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Detailed Vector Information: pHR-MMPCreGFP

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pHR-MMPCreGFP              
Source: David Livingston
Description: Self-excising mammalian retroviral vector with Cre-GFP enzyme-marker and LoxP sites; ampicillin resistance
Comments: None
Publications: PMID: 11511376
Title: Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity
Authors: Daniel Silver
Daniel Silver
David Livingston
EvNO00013284

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AVAILABLE
CPD only
Special MTA: CRE

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
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Vector Name: pHR-MMPCreGFP

pHR-MMPCreGFP in advanced viewed

Synonyms: pHR-MMP-Cre-GFP, pHR-MMP-CreGFP, pHit-and-Run, pMMP-Hit-and-run-CreGFP, pMMPHit and Run CreGFP
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: Self-excising mammalian retroviral vector with Cre-GFP enzyme-marker and LoxP sites; ampicillin resistance
Comments: Vector donor notes fussy to grow; try RecA+ strain and/or growth for 1 hour without selection to give it a boost.
Size (bp): 8142
Parent Vector: None
Properties: fluorescent marker, loxP, mammalian expression, mammalian transduction, retroviral vector, with tag/fusion/marker
Author Name: Daniel Silver
Publications: PMID: 11511376
Title: Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
Cre coding region Cre-GFP Cre-GFP fusion protein coding region 2428 4209
bacterial origin ori bacterial origin of replication (inferred) 0 0
fluorescent marker Cre-GFP Cre-GFP fusion protein coding region 2428 4209
recombination site loxP loxP recombination site (LOX 511) 0 0
selectable marker AmpR ampicillin resistance gene 6687 7544
viral LTR U3-R-U5 U3-R-U5 LTR for retroviral integration 0 0

 

SYNONYM: pHit-n-Run
SYNONYM: HR-MMPCreGFP
SYNONYM: pMMPHit and Run CreGFP
SYNONYM: pHR-MMP-CreGFP
SYNONYM: pHR-MMP-Cre-GFP
SYNONYM: pMMP-Hit-and-run-CreGFP
SYNONYM: pHR-MMPCreGFP
Species Specific ID: None
Target GenBank: None