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HOXB7 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  HOXB7
Gene Name:  homeobox B7
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH054437.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR201               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00001351

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 654nts         Open reading frame : 1 to 654
Reference Sequence Alignment

ATGAGTTCATTGTATTATGCGAATGCTTTATTTTCTAAATATCCAGCCTCAAGTTCGGTT
TTCGCTACCGGAGCCTTCCCAGAACAAACTTCTTGTGCGTTTGCTTCCAACCCCCAGCGC
CCGGGCTATGGAGCGGGTTCGGGCGCTTCCTTCGCCGCCTCGATGCAGGGCTTGTACCCC
GGCGGGGGGGGCATGGCGGGCCAGAGCGCGGCCGGCGTCTACGCGGCCGGCTATGGGCTC
GAGCCGAGTTCCTTCAACATGCACTGCGCGCCCTTTGAGCAGAACCTCTCCGGGGTGTGT
CCCGGCGACTCCGCCAAGGCGGCGGGCGCCAAGGAGCAGAGGGACTCGGACTTGGCGGCC
GAGAGTAACTTCCGGATCTACCCCTGGATGCGAAGCTCAGGAACTGACCGCAAACGAGGC
CGCCAGACCTACACCCGCTACCAGACCCTGGAGCTGGAGAAAGAATTTCACTACAATCGC
TACCTGACGCGGCGGCGGCGCATCGAGATCGCGCACACGCTCTGCCTCACGGAAAGACAG
ATCAAGATTTGGTTTCAGAACCGGCGCATGAAGTGGAAAAAGGAGAACAAGACCGCGGGC
CCGGGGACCACCGGCCAAGACAGGGCTGAAGCAGAGGAGGAAGAGGAAGAGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 704
Start on reference sequence 51
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
(goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR201

pDONR201 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  pDONR201-forward
Reverse:  pDONR201-reverse
Description: Recombinational donor/master vector; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4470
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 (pUC-type) origin of replication (modified, low copy number) 3744 4426
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1264 959
primer site forward primer recommended forward primer site 5 tcgcgttaacgctagcatggatctc 3 300 324
primer site reverse primer recommended reverse primer site 5 gtaacatcagagattttgagacac 3 2769 2792
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2744 2513
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 332 563
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2744 2513
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 332 563
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2265 2513
selectable marker KanR kanamycin resistance gene 2868 3677
trxn termination sequence rrn T2 rrn T2 transcription termination sequence 73 100
trxn termination sequence rrn T1 rrn T1 transcription termination sequence 232 275

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : HOXB7
Symbol Nomenclature : HOXB7
SYNONYM : HHO.C1; HOX2; HOX2C; Hox-2.3
Designation : homeo box 2C|homeo box B7|homeo box c1 protein|homeobox protein HHO.C1|homeobox protein Hox-2C|homeobox protein Hox-B7
Full Nomenclature : homeobox B7
GENEID : 3217
GI : 60816678
GenBank Accession : AY893355
HGNC : 5118
MIM : 142962
Vega : OTTHUMG00000170231
Target GenBank: BC015345

 

Reference Sequence Alignment

  

HsCD00001351      ATGAGTTCATTGTATTATGCGAATGCTTTATTTTCTAAATATCCAGCCTCAAGTTCGGTT
NM_004502.3       ATGAGTTCATTGTATTATGCGAATACTTTATTTTCTAAATATCCAGCCTCAAGTTCGGTT
                  ************************.***********************************

HsCD00001351      TTCGCTACCGGAGCCTTCCCAGAACAAACTTCTTGTGCGTTTGCTTCCAACCCCCAGCGC
NM_004502.3       TTCGCTACCGGAGCCTTCCCAGAACAAACTTCTTGTGCGTTTGCTTCCAACCCCCAGCGC
                  ************************************************************

HsCD00001351      CCGGGCTATGGAGCGGGTTCGGGCGCTTCCTTCGCCGCCTCGATGCAGGGCTTGTACCCC
NM_004502.3       CCGGGCTATGGAGCGGGTTCGGGCGCTTCCTTCGCCGCCTCGATGCAGGGCTTGTACCCC
                  ************************************************************

HsCD00001351      GGCGGGGGGGGCATGGCGGGCCAGAGCGCGGCCGGCGTCTACGCGGCCGGCTATGGGCTC
NM_004502.3       GGCGGGGGGGGCATGGCGGGCCAGAGCGCGGCCGGCGTCTACGCGGCCGGCTATGGGCTC
                  ************************************************************

HsCD00001351      GAGCCGAGTTCCTTCAACATGCACTGCGCGCCCTTTGAGCAGAACCTCTCCGGGGTGTGT
NM_004502.3       GAGCCGAGTTCCTTCAACATGCACTGCGCGCCCTTTGAGCAGAACCTCTCCGGGGTGTGT
                  ************************************************************

HsCD00001351      CCCGGCGACTCCGCCAAGGCGGCGGGCGCCAAGGAGCAGAGGGACTCGGACTTGGCGGCC
NM_004502.3       CCCGGCGACTCCGCCAAGGCGGCGGGCGCCAAGGAGCAGAGGGACTCGGACTTGGCGGCC
                  ************************************************************

HsCD00001351      GAGAGTAACTTCCGGATCTACCCCTGGATGCGAAGCTCAGGAACTGACCGCAAACGAGGC
NM_004502.3       GAGAGTAACTTCCGGATCTACCCCTGGATGCGAAGCTCAGGAACTGACCGCAAACGAGGC
                  ************************************************************

HsCD00001351      CGCCAGACCTACACCCGCTACCAGACCCTGGAGCTGGAGAAAGAATTTCACTACAATCGC
NM_004502.3       CGCCAGACCTACACCCGCTACCAGACCCTGGAGCTGGAGAAAGAATTTCACTACAATCGC
                  ************************************************************

HsCD00001351      TACCTGACGCGGCGGCGGCGCATCGAGATCGCGCACACGCTCTGCCTCACGGAAAGACAG
NM_004502.3       TACCTGACGCGGCGGCGGCGCATCGAGATCGCGCACACGCTCTGCCTCACGGAAAGACAG
                  ************************************************************

HsCD00001351      ATCAAGATTTGGTTTCAGAACCGGCGCATGAAGTGGAAAAAGGAGAACAAGACCGCGGGC
NM_004502.3       ATCAAGATTTGGTTTCAGAACCGGCGCATGAAGTGGAAAAAGGAGAACAAGACCGCGGGC
                  ************************************************************

HsCD00001351      CCGGGGACCACCGGCCAAGACAGGGCTGAAGCAGAGGAGGAAGAGGAAGAGTAG
NM_004502.3       CCGGGGACCACCGGCCAAGACAGGGCTGAAGCAGAGGAGGAAGAGGAAGAGTGA
                  ****************************************************..

Protein

SMART domain : HOX : Homeodomain
UniProt : P09629
HPRD : 00850

Clone

Cloning Information : 54437
HIP Master Clone ID : 10698
Original Clone ID : FLH054437.01X

Organism


TAX_ID : 9606
Species Specific ID: 3217

Genome

Chromosome : 17
Map Location : 17q21.3
Ensembl : ENSG00000260027

Reagents

Labome : HOXB7-antibody