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TK2 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TK2
Gene Name:  thymidine kinase 2, mitochondrial
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH000200.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors: HIP
HsCD00000142

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 705nts         Open reading frame : 1 to 705
Reference Sequence Alignment

ATGGGTGCGTTCTGCCAGCGTCCTAGCAGTGATAAAGAACAGGAAAAAGAGAAAAAATCA
GTGATCTGTGTCGAGGGCAATATTGCAAGTGGGAAGACGACATGCCTGGAATTCTTCTCC
AACGCGACAGACGTCGAGGTGTTAACGGAGCCTGTGTCCAAGTGGAGAAATGTCCGTGGC
CACAATCCTCTGGGCCTGATGTACCACGATGCCTCTCGCTGGGGTCTTACGCTACAGACT
TATGTGCAGCTCACCATGCTGGACAGGCATACTCGTCCTCAGGTGTCATCTGTACGGTTG
ATGGAGAGGTCGATTCACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAGTGGG
AAGATGCCAGAAGTGGACTATGTAGTTCTGTCGGAATGGTTTGACTGGATCTTGAGGAAC
ATGGACGTGTCTGTTGATTTGATAGTTTACCTTCGGACCAATCCTGAGACTTGTTACCAG
AGGTTAAAGAAGAGATGCAGGGAAGAGGAGAAGGTCATTCCGCTGGAATACCTGGAAGCA
ATTCACCATCTCCATGAGGAGTGGCTCATCAAAGGCAGCCTTTTCCCCATGGCAGCCCCT
GTTCTGGTGATTGAGGCTGACCACCACATGGAGAGGATGTTAGAACTCTTTGAACAAAAT
CGGGATCGAATATTAACTCCAGAGAATCGGAAGCATTGCCCATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 713
Start on reference sequence 9
Mutations:
None
Discrepancy:
g88a,G30S
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TK2
Symbol Nomenclature : TK2
SYNONYM : MTDPS2; MTTK
Designation : mt-TK
Full Nomenclature : thymidine kinase 2, mitochondrial
GENEID : 7084
GI : 33303982
GenBank Accession : AY335672
HGNC : 11831
MIM : 188250
Target GenBank: NM_004614

 

Reference Sequence Alignment

  

HsCD00000142        ------------------------------------------------------------
NM_004614.4         ATGCTGCTGTGGCCGCTGCGGGGCTGGGCCGCCCGGGCGCTGCGCTGCTTTGGGCCGGGA
NM_001172645.1      ------------------------------------------------------------
NM_001172643.1      ------------------------------------------------------------
NM_001271934.1      ------------------------------------------------------------
NM_001172644.1      ------------------------------------------------------------
                                                                                

HsCD00000142        ---------------------------------ATGGGTGCGTTCTGCCAGCGTCCTAGC
NM_004614.4         AGTCGCGGGAGCCCGGCCTCAGGCCCCGGGCCGCGGAGGGTGCAGCGCCGGGCCTGGCCT
NM_001172645.1      -------------------------ATGCTGCTGTGGCCGCTGCGGGGCTGGGCCGCCCG
NM_001172643.1      ---------------------------------ATGGGTGCGTTCTGCCAGCGTCCTAGC
NM_001271934.1      ------------------------------------------------------------
NM_001172644.1      -------------------------ATGCTGCTGTGGCCGCTGCGGGGCTGGGCCGCCCG
                                                                                

HsCD00000142        AGTGATAAAGAACAGGAAAAAGAGAAAAAATCAGTGATCTGTGTCGAGGGCAATATTGCA
NM_004614.4         CCCGATAAAGAACAGGAAAAAGAGAAAAAATCAGTGATCTGTGTCGAGGGCAATATTGCA
NM_001172645.1      GGCGCTGCGCTGCTTTGGGCCGGGAAGTCGCGGGAGCCCGGCCTCAGGCCCCGGGCCGCG
NM_001172643.1      AGTGATAAAGAACAGGAAAAAGAGAAAAAATCAGTGATCTGTGTCGAGGGCAATATTGCA
NM_001271934.1      ---------------------------ATGACAAGTATCTGTGTCGAGGGCAATATTGCA
NM_001172644.1      GGCGCTGCGCTGCTTTGGGCCGGGAAGTCGCGGGAGCCCGGCCTCAGGCCCCGGGCCGCG
                                               : .  ..  . * *  **..*  *.. .  **.

HsCD00000142        AGTGGGAAGACGACATGCCTGGAATTCTTCTCCAACGCGACAGACGTCGAGGTGTTAACG
NM_004614.4         AGTGGGAAGACGACATGCCTGGAATTCTTCTCCAACGCGACAGACGTCGAGGTGTTAACG
NM_001172645.1      GAGGGTGCAGCGCCGGGCCTGGCCTCCCGATAAAGAACAGGAAAAAGAGAAAAAATCAGT
NM_001172643.1      AGTGGGAAGACGACATGCCTGGAATTCTTCTCCAACGCGACAGACGTCGAGGTGTTAACG
NM_001271934.1      AGTGGGAAGACGACATGCCTGGAATTCTTCTCCAACGCGACAGACGTCGAGGTGTTAACG
NM_001172644.1      GAGGGTGCAGCGCCGGGCCTGGCCTCCCGATAAAGAACAGGAAAAAGAGAAAAAATCAGT
                    .. ** ....**.*. ******..* *  .*..*...*.. *.*.. .**..:.:*.*  

HsCD00000142        -------------------------------GAGCCTGTGTCCAAGTGGAGAAATGTCCG
NM_004614.4         -------------------------------GAGCCTGTGTCCAAGTGGAGAAATGTCCG
NM_001172645.1      GATCTGTGTCGAGGGCAATATTGCAAGTGGGAAGACGACATGCCTGGAATTCTTCTCCAA
NM_001172643.1      -------------------------------GAGCCTGTGTCCAAGTGGAGAAATGTCCG
NM_001271934.1      -------------------------------GAGCCTGTGTCCAAGTGGAGAAATGTCCG
NM_001172644.1      GGTGTTAACGGAG---------------------CCTGTGTCCAAGTGGAGAAATGTCCG
                                                      .* . .* *.:* ..: .::   *..

HsCD00000142        TGGCCACAATCCTCTGGGCCTGATGTACCACGATGCCTCTCGCTGGGGTCTTACGCTACA
NM_004614.4         TGGCCACAATCCTCTGGGCCTGATGTACCACGATGCCTCTCGCTGGGGTCTTACGCTACA
NM_001172645.1      CGCGACAGACGTCGAGGGCCTGATGTACCACGATGCCTCTCGCTGGGGTCTTACGCTACA
NM_001172643.1      TGGCCACAATCCTCTGGGCCTGATGTACCACGATGCCTCTCGCTGGGGTCTTACGCTACA
NM_001271934.1      TGGCCACAATCCTCTGGGCCTGATGTACCACGATGCCTCTCGCTGGGGTCTTACGCTACA
NM_001172644.1      TGGCCACAATCCTCTGGGCCTGATGTACCACGATGCCTCTCGCTGGGGTCTTACGCTACA
                     *  ....*     :*********************************************

HsCD00000142        GACTTATGTGCAGCTCACCATGCTGGACAGGCATACTCGTCCTCAGGTGTCATCTGTACG
NM_004614.4         GACTTATGTGCAGCTCACCATGCTGGACAGGCATACTCGTCCTCAGGTGTCATCTGTACG
NM_001172645.1      GACTTATGTGCAGCTCACCATGCTGGACAGGCATACTCGTCCTCAGGTGTCATCTGTACG
NM_001172643.1      GACTTATGTGCAGCTCACCATGCTGGACAGGCATACTCGTCCTCAGGTGTCATCTGTACG
NM_001271934.1      GACTTATGTGCAGCTCACCATGCTGGACAGGCATACTCGTCCTCAGGTGTCATCTGTACG
NM_001172644.1      GACTTATGTGCAGCTCACCATGCTGGACAGGCATACTCGTCCTCAGGTGTCATCTGTACG
                    ************************************************************

HsCD00000142        GTTGATGGAGAGGTCGATTCACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAG
NM_004614.4         GTTGATGGAGAGGTCGATTCACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAG
NM_001172645.1      GTTGATGGAGAGGTCGATTCACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAG
NM_001172643.1      GTTGATGGAGAGGTCGATTCACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAG
NM_001271934.1      GTTGATGGAGAGGTCGATTCACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAG
NM_001172644.1      GTTGATGGAGAGGTCGATTCACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAG
                    ************************************************************

HsCD00000142        TGGGAAGATGCCAGAAGTGGACTATGTAGTTCTGTCGGAATGGTTTGACTGGATCTTGAG
NM_004614.4         TGGGAAGATGCCAGAAGTGGACTATGTAGTTCTGTCGGAATGGTTTGACTGGATCTTGAG
NM_001172645.1      TGGGAAGATGCCAGAAGTGGACTATGTAGTTCTGTCGGAATGGTTTGACTGGATCTTGAG
NM_001172643.1      TGGGAAGATGCCAGAAGTGGACTATGTAGTTCTGTCGGAATGGTTTGACTGGATCTTGAG
NM_001271934.1      TGGGAAGATGCCAGAAGTGGACTATGTAGTTCTGTCGGAATGGTTTGACTGGATCTTGAG
NM_001172644.1      TGGGAAGATGCCAGAAGTGGACTATGTAGTTCTGTCGGAATGGTTTGACTGGATCTTGAG
                    ************************************************************

HsCD00000142        GAACATGGACGTGTCTGTTGATTTGATAGTTTACCTTCGGACCAATCCTGAGACTTGTTA
NM_004614.4         GAACATGGACGTGTCTGTTGATTTGATAGTTTACCTTCGGACCAATCCTGAGACTTGTTA
NM_001172645.1      GAACATGGACGTGTCTGTTGATTTGATAGTTTACCTTCGGACCAATCCTGAGACTTGTTA
NM_001172643.1      GAACATGGACGTGTCTGTTGATTTGATAGTTTACCTTCGGACCAATCCTGAGACTTGTTA
NM_001271934.1      GAACATGGACGTGTCTGTTGATTTGATAGTTTACCTTCGGACCAATCCTGAGACTTGTTA
NM_001172644.1      GAACATGGACGTGTCTGTTGATTTGATAGTTTACCTTCGGACCAATCCTGAGACTTGTTA
                    ************************************************************

HsCD00000142        CCAGAGGTTAAAGAAGAGATGCAGGGAAGAGGAGAAGGTCATTCCGCTGGAATACCTGGA
NM_004614.4         CCAGAGGTTAAAGAAGAGATGCAGGGAAGAGGAGAAGGTCATTCCGCTGGAATACCTGGA
NM_001172645.1      CCAGAGGTTAAAGAAGAGATGCAGGGAAGAGGAGAAGGTCATTCCGCTGGAATACCTGGA
NM_001172643.1      CCAGAGGTTAAAGAAGAGATGCAGGGAAGAGGAGAAGGTCATTCCGCTGGAATACCTGGA
NM_001271934.1      CCAGAGGTTAAAGAAGAGATGCAGGGAAGAGGAGAAGGTCATTCCGCTGGAATACCTGGA
NM_001172644.1      CCAGAGGTTAAAGAAGAGATGCAGGGAAGAGGAGAAGGTCATTCCGCTGGAATACCTGGA
                    ************************************************************

HsCD00000142        AGCAATTCACCATCTCCATGAGGAGTGGCTCATCAAAGGCAGCCTTTTCCCCATGGCAGC
NM_004614.4         AGCAATTCACCATCTCCATGAGGAGTGGCTCATCAAAGGCAGCCTTTTCCCCATGGCAGC
NM_001172645.1      AGCAATTCACCATCTCCATGAGGAGTGGCTCATCAAAGGCAGCCTTTTCCCCATGGCAGC
NM_001172643.1      AGCAATTCACCATCTCCATGAGGAGTGGCTCATCAAAGGCAGCCTTTTCCCCATGGCAGC
NM_001271934.1      AGCAATTCACCATCTCCATGAGGAGTGGCTCATCAAAGGCAGCCTTTTCCCCATGGCAGC
NM_001172644.1      AGCAATTCACCATCTCCATGAGGAGTGGCTCATCAAAGGCAGCCTTTTCCCCATGGCAGC
                    ************************************************************

HsCD00000142        CCCTGTTCTGGTGATTGAGGCTGACCACCACATGGAGAGGATGTTAGAACTCTTTGAACA
NM_004614.4         CCCTGTTCTGGTGATTGAGGCTGACCACCACATGGAGAGGATGTTAGAACTCTTTGAACA
NM_001172645.1      CCCTGTTCTGGTGATTGAGGCTGACCACCACATGGAGAGGATGTTAGAACTCTTTGAACA
NM_001172643.1      CCCTGTTCTGGTGATTGAGGCTGACCACCACATGGAGAGGATGTTAGAACTCTTTGAACA
NM_001271934.1      CCCTGTTCTGGTGATTGAGGCTGACCACCACATGGAGAGGATGTTAGAACTCTTTGAACA
NM_001172644.1      CCCTGTTCTGGTGATTGAGGCTGACCACCACATGGAGAGGATGTTAGAACTCTTTGAACA
                    ************************************************************

HsCD00000142        AAATCGGGATCGAATATTAACTCCAGAGAATCGGAAGCATTGCCCATTG
NM_004614.4         AAATCGGGATCGAATATTAACTCCAGAGAATCGGAAGCATTGCCCATAG
NM_001172645.1      AAATCGGGATCGAATATTAACTCCAGAGAATCGGAAGCATTGCCCATAG
NM_001172643.1      AAATCGGGATCGAATATTAACTCCAGAGAATCGGAAGCATTGCCCATAG
NM_001271934.1      AAATCGGGATCGAATATTAACTCCAGAGAATCGGAAGCATTGCCCATAG
NM_001172644.1      AAATCGGGATCGAATATTAACTCCAGAGAATCGGAAGCATTGCCCATAG
                    ***********************************************:*

Pathways

NCI : E2F transcription factor network
NCI : Validated targets of C-MYC transcriptional activation
Panther : Salvage pyrimidine deoxyribonucleotides
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Pyrimidine metabolism
Reactome : Pyrimidine salvage reactions
WikiPathway : Fluoropyrimidine Activity

Protein

UniProt : P04183
HPRD : 01770

Clone

Cloning Information : 200
HIP Master Clone ID : 87232
Original Clone ID : FLH000200.01L

Organism


TAX_ID : 9606
Species Specific ID: 7083

Genome

Chromosome : 16
Map Location : 16q22-q23.1
Ensembl : ENSG00000166548

Reagents

Labome : TK2-antibody