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PSMC3 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  PSMC3
Gene Name:  proteasome (prosome, macropain) 26S subunit, ATPase, 3
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH010314.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00001561

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1215nts         Open reading frame : 1 to 1215
Reference Sequence Alignment

ATGTCCACGGAGGAGATCATCCAGCGCACACGGCTGCTGGACAGTGAGATCAAGATCATG
AAGAGTGAAGTGTTGAGAGTCACCCATGAGCTCCAAGCCATGAAGGACAAGATAAAAGAG
AACAGTGAGAAAATCAAAGTGAACAAGACCCTGCCGTACCTTGTCTCCAACGTCATCGAG
CTCCTGGATGTTGATCCTAATGACCAAGAGGAGGATGGTGCCAATATTGACCTGGACTCC
CAGAGGAAGGGCAAGTGTGCTGTGATCAAAACCTCTACACGACAGACGTACTTCCTTCCT
GTGATTGGGTTGGTGGATGCTGAAAAGCTAAAGCCAGGAGACCTGGTGGGTGTGAACAAA
GACTCCTATCTGATCCTGGAGACGCTGCCCACAGAGTATGACTCGCGGGTGAAGGCCATG
GAGGTAGACGAGAGGCCCACGGAGCAATACAGTGACATTGGGGGTTTGGACAAGCAGATC
CAGGAGCTGGTGGAGGCCATTGTCTTGCCAATGAACCACAAGGAGAAGTTTGAGAACTTG
GGGATCCAACCTCCAAAAGGGGTGCTGATGTATGGGCCCCCAGGGACGGGGAAGACCCTC
CTGGCCCGGGCCTGTGCCGCACAGACTAAGGCCACCTTCCTAAAGCTGGCTGGCCCCCAG
CTGGTGCAGATGTTCATTGGAGATGGTGCCAAGCTAGTCCGGGATGCCTTTGCCCTGGCC
AAGGAGAAAGCGCCCTCTATCATCTTCATTGATGAGTTGGATGCCATCGGCACCAAGCGC
TTTGACAGTGAGAAGGCTGGGGACCGGGAGGTGCAGAGGACAATGCTGGAGCTTCTGAAC
CAGCTGGATGGCTTCCAGCCCAACACCCAAGTTAAGGTAATTGCAGCCACAAACAGGGTG
GACATCCTGGACCCCGCCCTCCTCCGCTCGGGCCGCCTTGACCGCAAGATAGAGTTCCCG
ATGCCCAATGAGGAGGCCCGGGCCAGAATCATGCAGATCCACTCCCGAAAGATGAATGTC
AGTCCTGACGTGAACTACGAGGAGCTGGCCCGCTGCACAGATGACTTCAATGGGGCCCAG
TGCAAGGCTGTGTGTGTGGAGGCGGGCATGATCGCACTGCGCAGGGGTGCCACGGAGCTC
ACCCACGAGGACTACATGGAAGGCATCCTGGAGGTGCAGGCCAAGAAGAAAGCCAACCTA
CAATACTACGCCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1277
Start on reference sequence 63
Mutations:
None
Discrepancy:
g1120c,c1121g,A374R
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PSMC3
Symbol Nomenclature : PSMC3
SYNONYM : TBP1
Designation : 26S protease regulatory subunit 6A|26S proteasome AAA-ATPase subunit RPT5|TBP-1|Tat-binding protein 1|human immunodeficiency virus tat transactivator binding protein-1|proteasome 26S subunit ATPase 3|proteasome subunit P50
Full Nomenclature : proteasome (prosome, macropain) 26S subunit, ATPase, 3
GENEID : 5702
GI : 61368867
GenBank Accession : AY891323
HGNC : 9549
MIM : 186852
Vega : OTTHUMG00000167692
Target GenBank: NM_002804

 

Reference Sequence Alignment

  

HsCD00001561      ------------------------------------------------------------
NM_002804.4       ATGAATCTGCTGCCGAATATTGAGAGTCCAGTGACTCGGCAGGAGAAGATGGCGACCGTG
                                                                              

HsCD00001561      ---------------------------------------------ATGTCCACGGAGGAG
NM_002804.4       TGGGATGAGGCCGAGCAAGATGGAATTGGGGAGGAGGTGCTCAAGATGTCCACGGAGGAG
                                                               ***************

HsCD00001561      ATCATCCAGCGCACACGGCTGCTGGACAGTGAGATCAAGATCATGAAGAGTGAAGTGTTG
NM_002804.4       ATCATCCAGCGCACACGGCTGCTGGACAGTGAGATCAAGATCATGAAGAGTGAAGTGTTG
                  ************************************************************

HsCD00001561      AGAGTCACCCATGAGCTCCAAGCCATGAAGGACAAGATAAAAGAGAACAGTGAGAAAATC
NM_002804.4       AGAGTCACCCATGAGCTCCAAGCCATGAAGGACAAGATAAAAGAGAACAGTGAGAAAATC
                  ************************************************************

HsCD00001561      AAAGTGAACAAGACCCTGCCGTACCTTGTCTCCAACGTCATCGAGCTCCTGGATGTTGAT
NM_002804.4       AAAGTGAACAAGACCCTGCCGTACCTTGTCTCCAACGTCATCGAGCTCCTGGATGTTGAT
                  ************************************************************

HsCD00001561      CCTAATGACCAAGAGGAGGATGGTGCCAATATTGACCTGGACTCCCAGAGGAAGGGCAAG
NM_002804.4       CCTAATGACCAAGAGGAGGATGGTGCCAATATTGACCTGGACTCCCAGAGGAAGGGCAAG
                  ************************************************************

HsCD00001561      TGTGCTGTGATCAAAACCTCTACACGACAGACGTACTTCCTTCCTGTGATTGGGTTGGTG
NM_002804.4       TGTGCTGTGATCAAAACCTCTACACGACAGACGTACTTCCTTCCTGTGATTGGGTTGGTG
                  ************************************************************

HsCD00001561      GATGCTGAAAAGCTAAAGCCAGGAGACCTGGTGGGTGTGAACAAAGACTCCTATCTGATC
NM_002804.4       GATGCTGAAAAGCTAAAGCCAGGAGACCTGGTGGGTGTGAACAAAGACTCCTATCTGATC
                  ************************************************************

HsCD00001561      CTGGAGACGCTGCCCACAGAGTATGACTCGCGGGTGAAGGCCATGGAGGTAGACGAGAGG
NM_002804.4       CTGGAGACGCTGCCCACAGAGTATGACTCGCGGGTGAAGGCCATGGAGGTAGACGAGAGG
                  ************************************************************

HsCD00001561      CCCACGGAGCAATACAGTGACATTGGGGGTTTGGACAAGCAGATCCAGGAGCTGGTGGAG
NM_002804.4       CCCACGGAGCAATACAGTGACATTGGGGGTTTGGACAAGCAGATCCAGGAGCTGGTGGAG
                  ************************************************************

HsCD00001561      GCCATTGTCTTGCCAATGAACCACAAGGAGAAGTTTGAGAACTTGGGGATCCAACCTCCA
NM_002804.4       GCCATTGTCTTGCCAATGAACCACAAGGAGAAGTTTGAGAACTTGGGGATCCAACCTCCA
                  ************************************************************

HsCD00001561      AAAGGGGTGCTGATGTATGGGCCCCCAGGGACGGGGAAGACCCTCCTGGCCCGGGCCTGT
NM_002804.4       AAAGGGGTGCTGATGTATGGGCCCCCAGGGACGGGGAAGACCCTCCTGGCCCGGGCCTGT
                  ************************************************************

HsCD00001561      GCCGCACAGACTAAGGCCACCTTCCTAAAGCTGGCTGGCCCCCAGCTGGTGCAGATGTTC
NM_002804.4       GCCGCACAGACTAAGGCCACCTTCCTAAAGCTGGCTGGCCCCCAGCTGGTGCAGATGTTC
                  ************************************************************

HsCD00001561      ATTGGAGATGGTGCCAAGCTAGTCCGGGATGCCTTTGCCCTGGCCAAGGAGAAAGCGCCC
NM_002804.4       ATTGGAGATGGTGCCAAGCTAGTCCGGGATGCCTTTGCCCTGGCCAAGGAGAAAGCGCCC
                  ************************************************************

HsCD00001561      TCTATCATCTTCATTGATGAGTTGGATGCCATCGGCACCAAGCGCTTTGACAGTGAGAAG
NM_002804.4       TCTATCATCTTCATTGATGAGTTGGATGCCATCGGCACCAAGCGCTTTGACAGTGAGAAG
                  ************************************************************

HsCD00001561      GCTGGGGACCGGGAGGTGCAGAGGACAATGCTGGAGCTTCTGAACCAGCTGGATGGCTTC
NM_002804.4       GCTGGGGACCGGGAGGTGCAGAGGACAATGCTGGAGCTTCTGAACCAGCTGGATGGCTTC
                  ************************************************************

HsCD00001561      CAGCCCAACACCCAAGTTAAGGTAATTGCAGCCACAAACAGGGTGGACATCCTGGACCCC
NM_002804.4       CAGCCCAACACCCAAGTTAAGGTAATTGCAGCCACAAACAGGGTGGACATCCTGGACCCC
                  ************************************************************

HsCD00001561      GCCCTCCTCCGCTCGGGCCGCCTTGACCGCAAGATAGAGTTCCCGATGCCCAATGAGGAG
NM_002804.4       GCCCTCCTCCGCTCGGGCCGCCTTGACCGCAAGATAGAGTTCCCGATGCCCAATGAGGAG
                  ************************************************************

HsCD00001561      GCCCGGGCCAGAATCATGCAGATCCACTCCCGAAAGATGAATGTCAGTCCTGACGTGAAC
NM_002804.4       GCCCGGGCCAGAATCATGCAGATCCACTCCCGAAAGATGAATGTCAGTCCTGACGTGAAC
                  ************************************************************

HsCD00001561      TACGAGGAGCTGGCCCGCTGCACAGATGACTTCAATGGGGCCCAGTGCAAGGCTGTGTGT
NM_002804.4       TACGAGGAGCTGGCCCGCTGCACAGATGACTTCAATGGGGCCCAGTGCAAGGCTGTGTGT
                  ************************************************************

HsCD00001561      GTGGAGGCGGGCATGATCGCACTGCGCAGGGGTGCCACGGAGCTCACCCACGAGGACTAC
NM_002804.4       GTGGAGGCGGGCATGATCGCACTGCGCAGGGGTGCCACGGAGCTCACCCACGAGGACTAC
                  ************************************************************

HsCD00001561      ATGGAAGGCATCCTGGAGGTGCAGGCCAAGAAGAAAGCCAACCTACAATACTACGCCTTG
NM_002804.4       ATGGAAGGCATCCTGGAGGTGCAGGCCAAGAAGAAAGCCAACCTACAATACTACGCCTAG
                  **********************************************************:*

Pathways

Reactome : APC/C-mediated degradation of cell cycle proteins
Reactome : APC/C:Cdc20 mediated degradation of Securin
Reactome : APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome : APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
Reactome : AUF1 (hnRNP D0) destabilizes mRNA
Reactome : Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome : Activation of NF-kappaB in B cells
Reactome : Adaptive Immune System
Reactome : Antigen processing-Cross presentation
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Apoptosis
Reactome : Assembly of the pre-replicative complex
Reactome : Asymmetric localization of PCP proteins
Reactome : Autodegradation of Cdh1 by Cdh1:APC/C
Reactome : Autodegradation of the E3 ubiquitin ligase COP1
Reactome : CDK-mediated phosphorylation and removal of Cdc6
Reactome : CDT1 association with the CDC6:ORC:origin complex
Reactome : Cdc20:Phospho-APC/C mediated degradation of Cyclin A
Reactome : Cell Cycle
Reactome : Cell Cycle Checkpoints
Reactome : Cell Cycle, Mitotic
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Cross-presentation of soluble exogenous antigens (endosomes)
Reactome : Cyclin A:Cdk2-associated events at S phase entry
Reactome : Cyclin E associated events during G1/S transition
Reactome : DNA Replication
Reactome : DNA Replication Pre-Initiation
Reactome : Disease
Reactome : Downstream signaling events of B Cell Receptor (BCR)
Reactome : ER-Phagosome pathway
Reactome : G1/S DNA Damage Checkpoints
Reactome : G1/S Transition
Reactome : Gene Expression
Reactome : HIV Infection
Reactome : Host Interactions of HIV factors
Reactome : Immune System
Reactome : M Phase
Reactome : M/G1 Transition
Reactome : Metabolism
Reactome : Metabolism of RNA
Reactome : Metabolism of amino acids and derivatives
Reactome : Metabolism of mRNA
Reactome : Mitotic Anaphase
Reactome : Mitotic G1-G1/S phases
Reactome : Mitotic M-M/G1 phases
Reactome : Mitotic Metaphase and Anaphase
Reactome : Orc1 removal from chromatin
Reactome : PCP/CE pathway
Reactome : Regulation of APC/C activators between G1/S and early anaphase
Reactome : Regulation of Apoptosis
Reactome : Regulation of DNA replication
Reactome : Regulation of activated PAK-2p34 by proteasome mediated degradation
Reactome : Regulation of mRNA stability by proteins that bind AU-rich elements
Reactome : Regulation of mitotic cell cycle
Reactome : Regulation of ornithine decarboxylase (ODC)
Reactome : Removal of licensing factors from origins
Reactome : S Phase
Reactome : SCF(Skp2)-mediated degradation of p27/p21
Reactome : SCF-beta-TrCP mediated degradation of Emi1
Reactome : Separation of Sister Chromatids
Reactome : Signal Transduction
Reactome : Signaling by Wnt
Reactome : Signaling by the B Cell Receptor (BCR)
Reactome : Stabilization of p53
Reactome : Switching of origins to a post-replicative state
Reactome : Synthesis of DNA
Reactome : TCF dependent signaling in response to WNT
Reactome : Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
Reactome : Ubiquitin-dependent degradation of Cyclin D
Reactome : Ubiquitin-dependent degradation of Cyclin D1
Reactome : Vif-mediated degradation of APOBEC3G
Reactome : Vpu mediated degradation of CD4
Reactome : beta-catenin independent WNT signaling
Reactome : degradation of AXIN
Reactome : degradation of DVL
Reactome : p53-Dependent G1 DNA Damage Response
Reactome : p53-Dependent G1/S DNA damage checkpoint
Reactome : p53-Independent DNA Damage Response
Reactome : p53-Independent G1/S DNA damage checkpoint
WikiPathway : Proteasome Degradation

Clone

Cloning Information : 10314
HIP Master Clone ID : 14451
Original Clone ID : FLH010314.01L

Organism


TAX_ID : 9606
Species Specific ID: 5702

Genome

Chromosome : 11
Map Location : 11p11.2
Ensembl : ENSG00000165916

Reagents

Labome : PSMC3-antibody