DNASU Plasmid Repository • 480.965.5697 | Email

YES1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  YES1
Gene Name:  v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH000152.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors: HIP
HsCD00001597

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1632nts         Open reading frame : 1 to 1632
Reference Sequence Alignment

ATGGGCTGCATTAAAAGTAAAGAAAACAAAAGTCCAGCCATTAAATACAGACCTGAAAAT
ACTCCAGAGCCTGTCAGTACAAGTGTGAGCCATTATGGAGCAGAACCCACTACAGTGTCA
CCATGTCCGTCATCTTCAGCAAAGGGAACAGCAGTTAATTTCAGCAGTCTTTCCATGACA
CCATTTGGAGGATCCTCAGGGGTAACGCCTTTTGGAGGTGCATCTTCCTCATTTTCAGTG
GTGCCAAGTTCATATCCTGCTGGTTTAACAGGTGGTGTTACTATATTTGTGGCCTTATAT
GATTATGAAGCTAGAACTACAGAAGACCTTTCATTTAAGAAGGGTGAAAGATTTCAAATA
ATTAACAATACGGAAGGAGATTGGTGGGAAGCAAGATCAATCGCTACAGGAAAGAATGGT
TATATCCCGAGCAATTATGTAGCGCCTGCAGATTCCATTCAGGCAGAAGAATGGTATTTT
GGCAAAATGGGGAGAAAAGATGCTGAAAGATTACTTTTGAATCCTGGAAATCAACGAGGT
ATTTTCTTAGTAAGAGAGAGTGAAACAACTAAAGGTGCTTATTCCCTTTCTATTCGTGAT
TGGGATGAGATAAGGGGTGACAATGTGAAACACTACAAAATTAGGAAACTTGACAATGGT
GGATACTATATCACAACCAGAGCACAATTTGATACTCTGCAGAAATTGGTGAAACACTAC
ACAGAACATGCTGATGGTTTATGCCACAAGTTGACAACTGTGTGTCCAACTGTGAAACCT
CAGACTCAAGGTCTAGCAAAAGATGCTTGGGAAATCCCTCGAGAATCTTTGCGACTAGAG
GTTAAACTAGGACAAGGATGTTTCGGCGAAGTGTGGATGGGAACATGGAATGGAACCACG
AAAGTAGCAATCAAAACACTAAAACCAGGTACAATGATGCCAGAAGCTTTCCTTCAAGAA
GCTCAGATAATGAAAAAATTAAGACATGATAAACTTGTTCCACTATATGCTGTTGTTTCT
GAAGAACCAATTTACATTGTCACTGAATTTATGTCAAAAGGAAGCTTATTAGATTTCCTT
AAGGAAGGAGATGGAAAGTATTTGAAGCTTCCACAGCTGGTTGATATGGCTGCTCAGATT
GCTGATGGTATGGCATATATTGAAAGAATGAACTATATTCACCGAGATCTTCGGGCTGCT
AATATTCTTGTAGGAGAAAATCTTGTGTGCAAAATAGCAGACTTTGGTTTAGCAAGGTTA
ATTGAAGACAATGAATACACAGCAAGACAAGGTGCAAAATTTCCAATCAAATGGACAGCT
CCTGAAGCTGCACTGTATGGTCGGTTTACAATAAAGTCTGATGTCTGGTCATTTGGAATT
CTGCAAACAGAACTAGTAACAAAGGGCCGAGTGCCATATCCAGGTATGGTGAACCGTGAA
GTACTAGAACAAGTGGAGCGAGGATACAGGATGCCGTGCCCTCAGGGCTGTCCAGAATCC
CTCCATGAATTGATGAATCTGTGTTGGAAGAAGGACCCTGATGAAAGACCAACATTTGAA
TATATTCAGTCCTTCTTGGAAGACTACTTCACTGCTACAGAGCCACAGTACCAGCCAGGA
GAAAATTTATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1839
Start on reference sequence 208
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : YES1
Symbol Nomenclature : YES1
SYNONYM : HsT441; P61-YES; Yes; c-yes
Designation : Yamaguchi sarcoma oncogene|cellular yes-1 protein|proto-oncogene c-Yes|proto-oncogene tyrosine-protein kinase YES|tyrosine-protein kinase Yes
Full Nomenclature : v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
GENEID : 7525
GI : 33304022
GenBank Accession : AY335692
HGNC : 12841
MIM : 164880
Vega : OTTHUMG00000131472
Target GenBank: NM_005433

 

Reference Sequence Alignment

  

HsCD00001597      ATGGGCTGCATTAAAAGTAAAGAAAACAAAAGTCCAGCCATTAAATACAGACCTGAAAAT
NM_005433.3       ATGGGCTGCATTAAAAGTAAAGAAAACAAAAGTCCAGCCATTAAATACAGACCTGAAAAT
                  ************************************************************

HsCD00001597      ACTCCAGAGCCTGTCAGTACAAGTGTGAGCCATTATGGAGCAGAACCCACTACAGTGTCA
NM_005433.3       ACTCCAGAGCCTGTCAGTACAAGTGTGAGCCATTATGGAGCAGAACCCACTACAGTGTCA
                  ************************************************************

HsCD00001597      CCATGTCCGTCATCTTCAGCAAAGGGAACAGCAGTTAATTTCAGCAGTCTTTCCATGACA
NM_005433.3       CCATGTCCGTCATCTTCAGCAAAGGGAACAGCAGTTAATTTCAGCAGTCTTTCCATGACA
                  ************************************************************

HsCD00001597      CCATTTGGAGGATCCTCAGGGGTAACGCCTTTTGGAGGTGCATCTTCCTCATTTTCAGTG
NM_005433.3       CCATTTGGAGGATCCTCAGGGGTAACGCCTTTTGGAGGTGCATCTTCCTCATTTTCAGTG
                  ************************************************************

HsCD00001597      GTGCCAAGTTCATATCCTGCTGGTTTAACAGGTGGTGTTACTATATTTGTGGCCTTATAT
NM_005433.3       GTGCCAAGTTCATATCCTGCTGGTTTAACAGGTGGTGTTACTATATTTGTGGCCTTATAT
                  ************************************************************

HsCD00001597      GATTATGAAGCTAGAACTACAGAAGACCTTTCATTTAAGAAGGGTGAAAGATTTCAAATA
NM_005433.3       GATTATGAAGCTAGAACTACAGAAGACCTTTCATTTAAGAAGGGTGAAAGATTTCAAATA
                  ************************************************************

HsCD00001597      ATTAACAATACGGAAGGAGATTGGTGGGAAGCAAGATCAATCGCTACAGGAAAGAATGGT
NM_005433.3       ATTAACAATACGGAAGGAGATTGGTGGGAAGCAAGATCAATCGCTACAGGAAAGAATGGT
                  ************************************************************

HsCD00001597      TATATCCCGAGCAATTATGTAGCGCCTGCAGATTCCATTCAGGCAGAAGAATGGTATTTT
NM_005433.3       TATATCCCGAGCAATTATGTAGCGCCTGCAGATTCCATTCAGGCAGAAGAATGGTATTTT
                  ************************************************************

HsCD00001597      GGCAAAATGGGGAGAAAAGATGCTGAAAGATTACTTTTGAATCCTGGAAATCAACGAGGT
NM_005433.3       GGCAAAATGGGGAGAAAAGATGCTGAAAGATTACTTTTGAATCCTGGAAATCAACGAGGT
                  ************************************************************

HsCD00001597      ATTTTCTTAGTAAGAGAGAGTGAAACAACTAAAGGTGCTTATTCCCTTTCTATTCGTGAT
NM_005433.3       ATTTTCTTAGTAAGAGAGAGTGAAACAACTAAAGGTGCTTATTCCCTTTCTATTCGTGAT
                  ************************************************************

HsCD00001597      TGGGATGAGATAAGGGGTGACAATGTGAAACACTACAAAATTAGGAAACTTGACAATGGT
NM_005433.3       TGGGATGAGATAAGGGGTGACAATGTGAAACACTACAAAATTAGGAAACTTGACAATGGT
                  ************************************************************

HsCD00001597      GGATACTATATCACAACCAGAGCACAATTTGATACTCTGCAGAAATTGGTGAAACACTAC
NM_005433.3       GGATACTATATCACAACCAGAGCACAATTTGATACTCTGCAGAAATTGGTGAAACACTAC
                  ************************************************************

HsCD00001597      ACAGAACATGCTGATGGTTTATGCCACAAGTTGACAACTGTGTGTCCAACTGTGAAACCT
NM_005433.3       ACAGAACATGCTGATGGTTTATGCCACAAGTTGACAACTGTGTGTCCAACTGTGAAACCT
                  ************************************************************

HsCD00001597      CAGACTCAAGGTCTAGCAAAAGATGCTTGGGAAATCCCTCGAGAATCTTTGCGACTAGAG
NM_005433.3       CAGACTCAAGGTCTAGCAAAAGATGCTTGGGAAATCCCTCGAGAATCTTTGCGACTAGAG
                  ************************************************************

HsCD00001597      GTTAAACTAGGACAAGGATGTTTCGGCGAAGTGTGGATGGGAACATGGAATGGAACCACG
NM_005433.3       GTTAAACTAGGACAAGGATGTTTCGGCGAAGTGTGGATGGGAACATGGAATGGAACCACG
                  ************************************************************

HsCD00001597      AAAGTAGCAATCAAAACACTAAAACCAGGTACAATGATGCCAGAAGCTTTCCTTCAAGAA
NM_005433.3       AAAGTAGCAATCAAAACACTAAAACCAGGTACAATGATGCCAGAAGCTTTCCTTCAAGAA
                  ************************************************************

HsCD00001597      GCTCAGATAATGAAAAAATTAAGACATGATAAACTTGTTCCACTATATGCTGTTGTTTCT
NM_005433.3       GCTCAGATAATGAAAAAATTAAGACATGATAAACTTGTTCCACTATATGCTGTTGTTTCT
                  ************************************************************

HsCD00001597      GAAGAACCAATTTACATTGTCACTGAATTTATGTCAAAAGGAAGCTTATTAGATTTCCTT
NM_005433.3       GAAGAACCAATTTACATTGTCACTGAATTTATGTCAAAAGGAAGCTTATTAGATTTCCTT
                  ************************************************************

HsCD00001597      AAGGAAGGAGATGGAAAGTATTTGAAGCTTCCACAGCTGGTTGATATGGCTGCTCAGATT
NM_005433.3       AAGGAAGGAGATGGAAAGTATTTGAAGCTTCCACAGCTGGTTGATATGGCTGCTCAGATT
                  ************************************************************

HsCD00001597      GCTGATGGTATGGCATATATTGAAAGAATGAACTATATTCACCGAGATCTTCGGGCTGCT
NM_005433.3       GCTGATGGTATGGCATATATTGAAAGAATGAACTATATTCACCGAGATCTTCGGGCTGCT
                  ************************************************************

HsCD00001597      AATATTCTTGTAGGAGAAAATCTTGTGTGCAAAATAGCAGACTTTGGTTTAGCAAGGTTA
NM_005433.3       AATATTCTTGTAGGAGAAAATCTTGTGTGCAAAATAGCAGACTTTGGTTTAGCAAGGTTA
                  ************************************************************

HsCD00001597      ATTGAAGACAATGAATACACAGCAAGACAAGGTGCAAAATTTCCAATCAAATGGACAGCT
NM_005433.3       ATTGAAGACAATGAATACACAGCAAGACAAGGTGCAAAATTTCCAATCAAATGGACAGCT
                  ************************************************************

HsCD00001597      CCTGAAGCTGCACTGTATGGTCGGTTTACAATAAAGTCTGATGTCTGGTCATTTGGAATT
NM_005433.3       CCTGAAGCTGCACTGTATGGTCGGTTTACAATAAAGTCTGATGTCTGGTCATTTGGAATT
                  ************************************************************

HsCD00001597      CTGCAAACAGAACTAGTAACAAAGGGCCGAGTGCCATATCCAGGTATGGTGAACCGTGAA
NM_005433.3       CTGCAAACAGAACTAGTAACAAAGGGCCGAGTGCCATATCCAGGTATGGTGAACCGTGAA
                  ************************************************************

HsCD00001597      GTACTAGAACAAGTGGAGCGAGGATACAGGATGCCGTGCCCTCAGGGCTGTCCAGAATCC
NM_005433.3       GTACTAGAACAAGTGGAGCGAGGATACAGGATGCCGTGCCCTCAGGGCTGTCCAGAATCC
                  ************************************************************

HsCD00001597      CTCCATGAATTGATGAATCTGTGTTGGAAGAAGGACCCTGATGAAAGACCAACATTTGAA
NM_005433.3       CTCCATGAATTGATGAATCTGTGTTGGAAGAAGGACCCTGATGAAAGACCAACATTTGAA
                  ************************************************************

HsCD00001597      TATATTCAGTCCTTCTTGGAAGACTACTTCACTGCTACAGAGCCACAGTACCAGCCAGGA
NM_005433.3       TATATTCAGTCCTTCTTGGAAGACTACTTCACTGCTACAGAGCCACAGTACCAGCCAGGA
                  ************************************************************

HsCD00001597      GAAAATTTATTG
NM_005433.3       GAAAATTTATAA
                  **********:.

Pathways

NCI : Alpha-synuclein signaling
NCI : CDC42 signaling events
NCI : CXCR4-mediated signaling events
NCI : Class I PI3K signaling events
NCI : EPHA forward signaling
NCI : Ephrin B reverse signaling
NCI : Glypican 1 network
NCI : Netrin-mediated signaling events
NCI : Noncanonical Wnt signaling pathway
NCI : PDGFR-beta signaling pathway
NCI : Regulation of p38-alpha and p38-beta
NCI : Signaling events mediated by PTP1B
NCI : Signaling events mediated by focal adhesion kinase
NCI : Thromboxane A2 receptor signaling
NCI : amb2 Integrin signaling
Panther : Cadherin signaling pathway
Panther : Parkinson disease
Reactome : Adaptive Immune System
Reactome : CD28 co-stimulation
Reactome : CTLA4 inhibitory signaling
Reactome : Cell surface interactions at the vascular wall
Reactome : Costimulation by the CD28 family
Reactome : Cytokine Signaling in Immune system
Reactome : FCGR activation
Reactome : Fcgamma receptor (FCGR) dependent phagocytosis
Reactome : Hemostasis
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Interleukin-3, 5 and GM-CSF signaling
Reactome : PECAM1 interactions
Reactome : Regulation of KIT signaling
Reactome : Regulation of signaling by CBL
Reactome : Signal Transduction
Reactome : Signaling by ERBB2
Reactome : Signaling by Interleukins
Reactome : Signaling by SCF-KIT

Clone

Cloning Information : 152
HIP Master Clone ID : 87210
Original Clone ID : FLH000152.01L

Organism


TAX_ID : 9606
Species Specific ID: 7525

Genome

Chromosome : 18
Map Location : 18p11.31-p11.21
Ensembl : ENSG00000176105

Reagents

Labome : YES1-antibody