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EIF3S6 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  EIF3E
Gene Name:  eukaryotic translation initiation factor 3, subunit E
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH031138.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00000165

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1338nts         Open reading frame : 1 to 1338
Reference Sequence Alignment

ATGGCGGAGTACGACTTGACTACTCGCATCGCGCACTTTTTGGATCGGCATCTAGTCTTT
CCGCTTCTTGAATTTCTCTCTGTAAAGGAGATATATAATGAAAAGGAATTATTACAAGGT
AAATTGGACCTTCTTAGTGATACCAACATGGTAGACTTTGCTATGGATGTATACAAAAAC
CTTTATTCTGATGATATTCCTCATGCTTTGAGAGAGAAAAGAACCACAGTGGTTGCACAA
CTGAAACAGCTTCAGGCAGAAACAGAACCAATTGTGAAGATGTTTGAAGATCCAGAAACT
ACAAGGCAAATGCAGTCAACCAGGGATGGTAGGATGCTCTTTGACTACCTGGCGGACAAG
CATGGTTTTAGGCAGGAATATTTAGATACACTCTACAGATATGCAAAATTCCAGTACGAA
TGTGGGAATTACTCAGGAGCAGCAGAATATCTTTATTTTTTTAGAGTGCTGGTTCCAGCA
ACAGATAGAAATGCTTTAAGTTCACTCTGGGGAAAGCTGGCCTCTGAAATCTTAATGCAG
AATTGGGATGCAGCCATGGAAGACCTTACACGGTTAAAAGAGACCATAGATAATAATTCT
GTGAGTTCTCCACTTCAGTCTCTTCAGCAGAGAACATGGCTCATTCACTGGTCTCTGTTT
GTTTTCTTCAATCACCCCAAAGGTCGCGATAATATTATTGACCTCTTCCTTTATCAGCCA
CAATATCTTAATGCAATTCAGACAATGTGTCCACACATTCTTCGCTATTTGACTACAGCA
GTCATAACAAACAAGGATGTTCGAAAACGTCGGCAGGTTCTAAAAGATCTAGTTAAAGTT
ATTCAACAGGAGTCTTACACATATAAAGACCCAATTACAGAATTTGTTGAATGTTTATAT
GTTAACTTTGACTTTGATGGGGCTCAGAAAAAGCTGAGGGAATGTGAATCAGTGCTTGTG
AATGACTTCTTCTTGGTGGCTTGTCTTGAGGATTTCATTGAAAATGCCCGTCTCTTCATA
TTTGAGACTTTCTGTCGCATCCACCAGTGTATCAGCATTAACATGTTGGCAGATAAATTG
AACATGACTCCAGAAGAAGCTGAAAGGTGGATTGTAAATTTGATTAGAAATGCAAGACTG
GATGCCAAGATTGATTCTAAATTAGGTCATGTGGTTATGGGTAACAATGCAGTCTCACCC
TATCAGCAAGTGATTGAAAAGACCAAAAGCCTTTCCTTTAGAAGCCAGATGTTGGCCATG
AATATTGAGAAGAAACTTAATCAGAATAGCAGGTCAGAGGCTCCTAACTGGGCAACTCAA
GATTCTGGCTTCTACTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1360
Start on reference sequence 23
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : EIF3E
Symbol Nomenclature : EIF3E
SYNONYM : EIF3-P48; EIF3S6; INT6; eIF3-p46
Designation : eIF-3 p48|eukaryotic translation initiation factor 3 subunit 6|eukaryotic translation initiation factor 3 subunit E|eukaryotic translation initiation factor 3, subunit 6 (48kD)|eukaryotic translation initiation factor 3, subunit 6 48kDa|mammary tumor-associated protein INT6|murine mammary tumor integration site 6 (oncogene homolog)|viral integration site protein INT-6 homolog
Full Nomenclature : eukaryotic translation initiation factor 3, subunit E
GENEID : 3646
GI : 61355047
GenBank Accession : AY888146
HGNC : 3277
MIM : 602210
Vega : OTTHUMG00000164858
Target GenBank: NM_001568

 

Reference Sequence Alignment

  

HsCD00000165      ATGGCGGAGTACGACTTGACTACTCGCATCGCGCACTTTTTGGATCGGCATCTAGTCTTT
NM_001568.2       ATGGCGGAGTACGACTTGACTACTCGCATCGCGCACTTTTTGGATCGGCATCTAGTCTTT
                  ************************************************************

HsCD00000165      CCGCTTCTTGAATTTCTCTCTGTAAAGGAGATATATAATGAAAAGGAATTATTACAAGGT
NM_001568.2       CCGCTTCTTGAATTTCTCTCTGTAAAGGAGATATATAATGAAAAGGAATTATTACAAGGT
                  ************************************************************

HsCD00000165      AAATTGGACCTTCTTAGTGATACCAACATGGTAGACTTTGCTATGGATGTATACAAAAAC
NM_001568.2       AAATTGGACCTTCTTAGTGATACCAACATGGTAGACTTTGCTATGGATGTATACAAAAAC
                  ************************************************************

HsCD00000165      CTTTATTCTGATGATATTCCTCATGCTTTGAGAGAGAAAAGAACCACAGTGGTTGCACAA
NM_001568.2       CTTTATTCTGATGATATTCCTCATGCTTTGAGAGAGAAAAGAACCACAGTGGTTGCACAA
                  ************************************************************

HsCD00000165      CTGAAACAGCTTCAGGCAGAAACAGAACCAATTGTGAAGATGTTTGAAGATCCAGAAACT
NM_001568.2       CTGAAACAGCTTCAGGCAGAAACAGAACCAATTGTGAAGATGTTTGAAGATCCAGAAACT
                  ************************************************************

HsCD00000165      ACAAGGCAAATGCAGTCAACCAGGGATGGTAGGATGCTCTTTGACTACCTGGCGGACAAG
NM_001568.2       ACAAGGCAAATGCAGTCAACCAGGGATGGTAGGATGCTCTTTGACTACCTGGCGGACAAG
                  ************************************************************

HsCD00000165      CATGGTTTTAGGCAGGAATATTTAGATACACTCTACAGATATGCAAAATTCCAGTACGAA
NM_001568.2       CATGGTTTTAGGCAGGAATATTTAGATACACTCTACAGATATGCAAAATTCCAGTACGAA
                  ************************************************************

HsCD00000165      TGTGGGAATTACTCAGGAGCAGCAGAATATCTTTATTTTTTTAGAGTGCTGGTTCCAGCA
NM_001568.2       TGTGGGAATTACTCAGGAGCAGCAGAATATCTTTATTTTTTTAGAGTGCTGGTTCCAGCA
                  ************************************************************

HsCD00000165      ACAGATAGAAATGCTTTAAGTTCACTCTGGGGAAAGCTGGCCTCTGAAATCTTAATGCAG
NM_001568.2       ACAGATAGAAATGCTTTAAGTTCACTCTGGGGAAAGCTGGCCTCTGAAATCTTAATGCAG
                  ************************************************************

HsCD00000165      AATTGGGATGCAGCCATGGAAGACCTTACACGGTTAAAAGAGACCATAGATAATAATTCT
NM_001568.2       AATTGGGATGCAGCCATGGAAGACCTTACACGGTTAAAAGAGACCATAGATAATAATTCT
                  ************************************************************

HsCD00000165      GTGAGTTCTCCACTTCAGTCTCTTCAGCAGAGAACATGGCTCATTCACTGGTCTCTGTTT
NM_001568.2       GTGAGTTCTCCACTTCAGTCTCTTCAGCAGAGAACATGGCTCATTCACTGGTCTCTGTTT
                  ************************************************************

HsCD00000165      GTTTTCTTCAATCACCCCAAAGGTCGCGATAATATTATTGACCTCTTCCTTTATCAGCCA
NM_001568.2       GTTTTCTTCAATCACCCCAAAGGTCGCGATAATATTATTGACCTCTTCCTTTATCAGCCA
                  ************************************************************

HsCD00000165      CAATATCTTAATGCAATTCAGACAATGTGTCCACACATTCTTCGCTATTTGACTACAGCA
NM_001568.2       CAATATCTTAATGCAATTCAGACAATGTGTCCACACATTCTTCGCTATTTGACTACAGCA
                  ************************************************************

HsCD00000165      GTCATAACAAACAAGGATGTTCGAAAACGTCGGCAGGTTCTAAAAGATCTAGTTAAAGTT
NM_001568.2       GTCATAACAAACAAGGATGTTCGAAAACGTCGGCAGGTTCTAAAAGATCTAGTTAAAGTT
                  ************************************************************

HsCD00000165      ATTCAACAGGAGTCTTACACATATAAAGACCCAATTACAGAATTTGTTGAATGTTTATAT
NM_001568.2       ATTCAACAGGAGTCTTACACATATAAAGACCCAATTACAGAATTTGTTGAATGTTTATAT
                  ************************************************************

HsCD00000165      GTTAACTTTGACTTTGATGGGGCTCAGAAAAAGCTGAGGGAATGTGAATCAGTGCTTGTG
NM_001568.2       GTTAACTTTGACTTTGATGGGGCTCAGAAAAAGCTGAGGGAATGTGAATCAGTGCTTGTG
                  ************************************************************

HsCD00000165      AATGACTTCTTCTTGGTGGCTTGTCTTGAGGATTTCATTGAAAATGCCCGTCTCTTCATA
NM_001568.2       AATGACTTCTTCTTGGTGGCTTGTCTTGAGGATTTCATTGAAAATGCCCGTCTCTTCATA
                  ************************************************************

HsCD00000165      TTTGAGACTTTCTGTCGCATCCACCAGTGTATCAGCATTAACATGTTGGCAGATAAATTG
NM_001568.2       TTTGAGACTTTCTGTCGCATCCACCAGTGTATCAGCATTAACATGTTGGCAGATAAATTG
                  ************************************************************

HsCD00000165      AACATGACTCCAGAAGAAGCTGAAAGGTGGATTGTAAATTTGATTAGAAATGCAAGACTG
NM_001568.2       AACATGACTCCAGAAGAAGCTGAAAGGTGGATTGTAAATTTGATTAGAAATGCAAGACTG
                  ************************************************************

HsCD00000165      GATGCCAAGATTGATTCTAAATTAGGTCATGTGGTTATGGGTAACAATGCAGTCTCACCC
NM_001568.2       GATGCCAAGATTGATTCTAAATTAGGTCATGTGGTTATGGGTAACAATGCAGTCTCACCC
                  ************************************************************

HsCD00000165      TATCAGCAAGTGATTGAAAAGACCAAAAGCCTTTCCTTTAGAAGCCAGATGTTGGCCATG
NM_001568.2       TATCAGCAAGTGATTGAAAAGACCAAAAGCCTTTCCTTTAGAAGCCAGATGTTGGCCATG
                  ************************************************************

HsCD00000165      AATATTGAGAAGAAACTTAATCAGAATAGCAGGTCAGAGGCTCCTAACTGGGCAACTCAA
NM_001568.2       AATATTGAGAAGAAACTTAATCAGAATAGCAGGTCAGAGGCTCCTAACTGGGCAACTCAA
                  ************************************************************

HsCD00000165      GATTCTGGCTTCTACTGA
NM_001568.2       GATTCTGGCTTCTACTGA
                  ******************

Pathways

NCI : HIF-2-alpha transcription factor network
Reactome : 3' -UTR-mediated translational regulation
Reactome : Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
Reactome : Cap-dependent Translation Initiation
Reactome : Eukaryotic Translation Initiation
Reactome : Formation of a pool of free 40S subunits
Reactome : Formation of the ternary complex, and subsequently, the 43S complex
Reactome : GTP hydrolysis and joining of the 60S ribosomal subunit
Reactome : Gene Expression
Reactome : L13a-mediated translational silencing of Ceruloplasmin expression
Reactome : Metabolism of proteins
Reactome : Ribosomal scanning and start codon recognition
Reactome : Translation
Reactome : Translation initiation complex formation
WikiPathway : Translation Factors

Clone

Cloning Information : 31138
HIP Master Clone ID : 61514
Original Clone ID : FLH031138.01X

Organism


TAX_ID : 9606
Species Specific ID: 3646

Genome

Chromosome : 8
Map Location : 8q22-q23
Ensembl : ENSG00000104408

Reagents

Labome : EIF3E-antibody