| Gene: |
Gene Symbol:
No insert Gene Name: No insert |
|---|---|
| Original Clone ID: | None |
| Type: | No insert |
| Vector Name: | pPRIME-TET-GFP-recipient |
| Source: | Steve Elledge |
| Description: | Lentiviral expression vector for shRNA expression with inducible (Tet) promoter, fluorescent (GFP) marker; amp resistance; MAGIC bacterial mating cloning. |
| Comments: | This is an 'empty vector' version pPRIME for MAGIC bacterial mating-based strategy (see PMID 15731760) |
| Publications: |
PMID: 16141338 Title: A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells PMID: 15731760 Title: MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules |
| Authors: |
Steve Elledge Frank Stegmeier |
|
EvNO00017254 Price: Login for Pricing AVAILABLE CPD only Special MTA: None |
| Distributed in bacterial strain : | BW28705I/pML300 EB5-alpha T1, T5 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
ampicillin Bacterial Selection Condition: 100 ug/mL ampicillin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Commonly used conditions for ampicillin resistant plasmid clones. |
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| Vector Name: | pPRIME-TET-GFP-recipient |
pPRIME-TET-GFP-recipient in advanced viewed |
| Synonyms: | pPRIME-TET-GFP-PheS-recipient, p126 | |
| Sequencing Primer: | Forward:
Unknown
Reverse: Unknown |
|
| Description: | Lentiviral expression vector for shRNA expression with inducible (Tet) promoter, fluorescent (GFP) marker; amp resistance; MAGIC bacterial mating cloning. | |
| Comments: | MAGIC mating recipient for PRIME approach (Elledge Lab) | |
| Size (bp): | 8350 | |
| Parent Vector: | None | |
| Properties: | MAGIC mating system, fluorescent marker, inducible expression, lentiviral infection, mammalian expression, mammalian transduction, retroviral vector, with tag/fusion/marker | |
| Author Name: |
Steve Elledge Frank Stegmeier |
|
| Publications: |
PMID:
16141338
Title: A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells PMID: 15731760 Title: MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules |
|
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| RNA regulatory element | WRE | woodchuck hepatitis B virus RNA regulatory element | 5279 | 5868 |
| bacterial origin | ori | bacterial origin of replication (inferred) | 0 | 0 |
| fluorescent marker | GFP | GFP | 2928 | 3648 |
| negative selection marker | PheS cassette | Includes conditional dominant Cl-Phe sensitive marker pheS Gly294 | 3695 | 5116 |
| promoter | TET | TET eukaryotic promoter | 2581 | 2914 |
| selectable marker | AmpR | ampicillin resistance gene | 0 | 0 |
| viral LTR | LTR | viral LTR | 5888 | 6577 |
| viral LTR | LTR | viral LTR | 835 | 1509 |
| viral response element | HIV RRE | HIV Rev Response Element | 1539 | 2396 |
| SYNONYM: |
p126 |
|---|---|
| SYNONYM: |
pPRIME-TET-GFP-recipient |
| SYNONYM: |
pPRIME-TET-GFP-PheS-recipient |
| Species Specific ID: | None |
| Target GenBank: | None |
