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Detailed Vector Information: pPRIME-TET-GIN-recipient

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pPRIME-TET-GIN-recipient              
Source: Steve Elledge
Description: Lentiviral expression vector for shRNA expression with inducible (Tet) promoter, bicistronic fluorescent and selectable markers (GFP-IRES-neoR); amp resistance (neomycin for mammalian selection); MAGIC bacterial mating cloning.
Comments: This is an 'empty vector' version pPRIME for MAGIC bacterial mating-based strategy (see PMID 15731760)
Publications: PMID: 16141338
Title: A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells
PMID: 15731760
Title: MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules
Authors: Steve Elledge
Frank Stegmeier
EvNO00017255

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : BW28705I/pML300 EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  neomycin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
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Vector Name: pPRIME-TET-GIN-recipient

pPRIME-TET-GIN-recipient in advanced viewed

Synonyms: p259, pPRIME-TET-GFP-IRES-NEO-recipient, pPRIME-TET-GIN-PheS-recipient
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: Lentiviral expression vector for shRNA expression with inducible (Tet) promoter, bicistronic fluorescent and selectable markers (GFP-IRES-neoR); amp resistance (neomycin for mammalian selection); MAGIC bacterial mating cloning.
Comments: MAGIC mating recipient for PRIME approach (Elledge Lab)
Size (bp): 9747
Parent Vector: None
Properties: MAGIC mating system, fluorescent marker, inducible expression, lentiviral infection, mammalian expression, mammalian transduction, retroviral vector, with tag/fusion/marker
Author Name: Steve Elledge
Frank Stegmeier
Publications: PMID: 16141338
Title: A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells

PMID: 15731760
Title: MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
IRES IRES-Neo Internal Ribosome Entry Site (with neo) 3655 5043
RNA regulatory element WRE woodchuck hepatitis B virus RNA regulatory element 6676 7265
bacterial origin ori bacterial origin of replication (inferred) 0 0
fluorescent marker GFP GFP 2928 3648
negative selection marker PheS cassette Includes conditional dominant Cl-Phe sensitive marker pheS Gly294 5092 6413
promoter TET TET eukaryotic promoter 2581 2914
selectable marker NeoR Neomycin resistance gene (follows IRES) 3655 5043
selectable marker AmpR ampicillin resistance gene 0 0
viral LTR LTR viral LTR 7285 7974
viral LTR LTR viral LTR 835 1509
viral response element HIV RRE HIV Rev Response Element 1539 2396

 

SYNONYM: p259
SYNONYM: pPRIME-TET-GIN-recipient
SYNONYM: pPRIME-TET-GFP-IRES-Neo-recipient
SYNONYM: pPRIME-TET-GIN-PheS-recipient
Species Specific ID: None
Target GenBank: None