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RBL2 (Homo sapiens) in pcDNA3

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Explanation of Terms


Gene: Gene Symbol:  RBL2
Gene Name:  retinoblastoma-like 2 (p130)
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pcDNA3               Format:  CLOSED
Source: James DeCaprio
Description: None
Comments: Insert is cloned into pcDNA3.1 Zeo vector (Invitrogen) and can be excised with Hind III/Apa I digest
Mutation/
Discrepancy :
No / Unknown
Publications: PMID: 11756542
Title: Nucleocytoplasmic shuttling of p130/RBL2: novel regulatory mechanism
Authors: James DeCaprio
Jocelyn Kasper
HsCD00017282

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: U Verification Method: 
Mutations:
None
Discrepancy:
None
Others:
Tag HA (N-terminal)
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  neomycin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pcDNA3

pcDNA3 in advanced viewed

Synonyms: pcDNA-3
Description: Mammalian expression vector with CMV promoter, BGH polyA signal, T7, SP6; amp resistance (neomycin for mammalian selection); restriction enzyme cloning.
Comments: None
Size (bp): 5446
Parent Vector: None
Empty Vector: None
Properties: in vitro transcription, mammalian expression, multiple cloning site, ssDNA production
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (HindIII KpnI BamHI BstXI EcoRI EcoRV BstXI NotI XhoI XbaI ApaI) 0 0
bacterial origin ColE1 ColE1 origin of replication 0 0
poly-A signal SV40 polyA SV40 polyadenylation signal sequence (for neoR) 0 0
poly-A signal BGH polyA BGH polyadenylation signal sequence 0 0
promoter SP6 SP6 prokaryotic promoter 0 0
promoter T7 T7 prokaryotic promoter 0 0
promoter CMV CMV promoter 0 0
promoter SV40 SV40 promoter (for neoR) 0 0
selectable marker AmpR ampicillin resistance 0 0
selectable marker NeoR neomycin resistance gene 0 0
viral origin SV40 SV40 origin (with SV40 promoter) 0 0

 


SYNONYM: HA-p130 (hs)
SYNONYM: HA-tagged human p130, full length
SYNONYM: DeCaprio_10
Species Specific ID: 5934
Target GenBank: NM_005611