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RBL1 (Homo sapiens) in pcDNA3

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Explanation of Terms


Gene: Gene Symbol:  RBL1
Gene Name:  retinoblastoma-like 1 (p107)
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pcDNA3               Format:  CLOSED
Source: James DeCaprio
Description: None
Comments: insert is cloned into pcDNA3 vector (Invitrogen) and can be excised with Hind III/Xba I digest
Mutation/
Discrepancy :
No / Unknown
Publications: PMID: 11756542
Title: Nucleocytoplasmic shuttling of p130/RBL2: novel regulatory mechanism
Authors: James DeCaprio
Jocelyn Kasper
HsCD00017283

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: U Verification Method: 
Mutations:
None
Discrepancy:
None
Others:
Tag HA (N-terminal)
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  neomycin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pcDNA3

pcDNA3 in advanced viewed

Synonyms: pcDNA-3
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: Mammalian expression vector with CMV promoter, BGH polyA signal, T7, SP6; amp resistance (neomycin for mammalian selection); restriction enzyme cloning.
Comments: None
Size (bp): 5446
Parent Vector: None
Empty Vector: None
Properties: in vitro transcription, mammalian expression, multiple cloning site, ssDNA production
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (HindIII KpnI BamHI BstXI EcoRI EcoRV BstXI NotI XhoI XbaI ApaI) 0 0
bacterial origin ColE1 ColE1 origin of replication 0 0
poly-A signal SV40 polyA SV40 polyadenylation signal sequence (for neoR) 0 0
poly-A signal BGH polyA BGH polyadenylation signal sequence 0 0
promoter SP6 SP6 prokaryotic promoter 0 0
promoter T7 T7 prokaryotic promoter 0 0
promoter CMV CMV promoter 0 0
promoter SV40 SV40 promoter (for neoR) 0 0
selectable marker AmpR ampicillin resistance 0 0
selectable marker NeoR neomycin resistance gene 0 0
viral origin SV40 SV40 origin (with SV40 promoter) 0 0

 

  • Gene
  • Pathways
  • Protein
  • Organism
  • Genome

Gene

 

Gene Symbol : RBL1
Symbol Nomenclature : RBL1
SYNONYM : CP107; PRB1; p107
Designation : 107 kDa retinoblastoma-associated protein|cellular protein 107|retinoblastoma-like protein 1
Full Nomenclature : retinoblastoma-like 1 (p107)
GENEID : 5933
Locus Tag : RP11-382A12.1
SYNONYM : HA-p107 (hs)
SYNONYM : HA-tagged human p107, full length
SYNONYM : DeCaprio_11
HGNC : 9893
MIM : 116957
Vega : OTTHUMG00000032406
Target GenBank: None

 

Pathways

NCI : E2F transcription factor network
NCI : Regulation of nuclear SMAD2/3 signaling
NCI : Validated targets of C-MYC transcriptional repression
Panther : p53 pathway feedback loops 2
Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Cyclin D associated events in G1
Reactome : Disease
Reactome : G0 and Early G1
Reactome : G1 Phase
Reactome : Gene Expression
Reactome : Generic Transcription Pathway
Reactome : Loss of Function of SMAD2/3 in Cancer
Reactome : Loss of Function of SMAD4 in Cancer
Reactome : Loss of Function of TGFBR1 in Cancer
Reactome : Loss of Function of TGFBR2 in Cancer
Reactome : Mitotic G1-G1/S phases
Reactome : SMAD2/3 MH2 Domain Mutants in Cancer
Reactome : SMAD2/3 Phosphorylation Motif Mutants in Cancer
Reactome : SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription
Reactome : SMAD4 MH2 Domain Mutants in Cancer
Reactome : Signal Transduction
Reactome : Signaling by TGF-beta Receptor Complex
Reactome : Signaling by TGF-beta Receptor Complex in Cancer
Reactome : TGFBR1 KD Mutants in Cancer
Reactome : TGFBR1 LBD Mutants in Cancer
Reactome : TGFBR2 Kinase Domain Mutants in Cancer
Reactome : TGFBR2 MSI Frameshift Mutants in Cancer
Reactome : Transcriptional activity of SMAD2/SMAD3:SMAD4 heterotrimer
WikiPathway : Adipogenesis
WikiPathway : Cell cycle
WikiPathway : G1 to S cell cycle control
WikiPathway : ID signaling pathway
WikiPathway : TGF beta Signaling Pathway

Protein

HPRD : 00312

Organism


TAX_ID : 9606
Species Specific ID: 5933

Genome

Chromosome : 20
Map Location : 20q11.2
Ensembl : ENSG00000080839