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TFDP2 (Homo sapiens) in pCMV4

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Explanation of Terms


Gene: Gene Symbol:  TFDP2
Gene Name:  transcription factor Dp-2 (E2F dimerization partner 2)
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pCMV4               Format:  CLOSED
Source: James DeCaprio
Description: None
Comments: insert is subcloned into pCMV vector
Mutation/
Discrepancy :
No / Unknown
Publications: PMID: 7739537
Title: In vivo association of E2F and DP family proteins
PMID: 9144196
Title: The subcellular localization of E2F-4 is cell-cycle dependent
Authors: James DeCaprio
Jocelyn Kasper
HsCD00017286

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AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: U Verification Method: 
Mutations:
None
Discrepancy:
None
Others:
Tag HA (N-terminal)
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pCMV4

pCMV4 in advanced viewed

Synonyms: pCMV-4
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: Mammalian expression vector with CMV promoter, AMV4-A translational enhancer; ampicillin resistance; restriction enzyme cloning.
Comments: None
Size (bp): 4900
Parent Vector: None
Empty Vector: None
Properties: mammalian expression, multiple cloning site
Author Name: S. Andersson
Publications: PMID: 2722778
Title: Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme

Vector Map:         Vector Sequence:
Sequence: Not Available

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (BglII, KpmI, MluI, ClaI, HindIII, XbaI, SmaI) 0 0
bacterial origin unknown ori unknown bacterial origin 0 0
phage origin F1 ori F1 phage origin for ssDNA production 0 0
poly-A signal hGH polyA human growth hormone polyadenylation signal sequence 0 0
promoter CMV human cytomegalovirus (CMV) promoter 0 0
selectable marker AmpR ampicillin resistance gene from E. coli 0 0
trln enhancer AMV4-A Alfalfa mosaic virus 4 RNA translational enhancer (A) 0 0
trxn termination sequence hGH trxn term human growth hormone gene transcription termination sequence 0 0
viral origin SV40 ori SV40 origin of DNA replication and early region enhancer sequences 0 0

 


SYNONYM: HA-DP-2(hs)
SYNONYM: HA-tagged human DP-2, full length
SYNONYM: DeCaprio_14
Species Specific ID: 7029
Target GenBank: NM_006286