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YWHAG (Homo sapiens) in pGEX


Explanation of Terms

Gene: Gene Symbol:  YWHAG
Gene Name:  tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pGEX               Format:  CLOSED
Source: James DeCaprio
Description: None
Comments: seems that a pGEX-T4 vector was used (see PMID 9428519)
Discrepancy :
No / Unknown
Publications: PMID: 9428519
Title: The structural basis for 14-3-3:phosphopeptide binding specificity
PMID: 15107609
Title: 14-3-3 family members act coordinately to regulate mitotic progression
PMID: 10330186
Title: Cytoplasmic localization of human cdc25C during interphase requires an intact 14-3-3 binding site
Authors: James DeCaprio
Jocelyn Kasper

Price:  Login for Pricing
CPD only
Special MTA: None


Coding Sequence Details
Insert Sequence Verified?: U Verification Method: 
Tag GST (from pGEX vector)
5' Linker Sequence: None
3' Linker Sequence: None


 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pGEX

pGEX in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: pGEX-series GST fusion vector with Ptac promoter, lacI repressor; amp resistance; restriction enzyme cloning.
Comments: Map shown is for later versions of pGEX and may not be accurate for the original construct.
Size (bp): 4900
Parent Vector: None
Empty Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Amersham Pharmacia
Publications: None
Vector Map:         Vector Sequence:
Sequence: Not Available


Vector Features:

Type Name Description Start Position End Position
MCS MCS BamHI, SmaI, EcoRI sites for in-frame cloning 0 0
bacterial origin ColE1 pBR322-type ColE1 origin 0 0
promoter Ptac Ptac promoter 0 0
selectable marker AmpR ampicillin resistance gene 0 0
tag GST glutathione S-transferase (GST) coding region 0 0


SYNONYM: GST 14-3-3 g
SYNONYM: GST 14-4-4 gamma
SYNONYM: DeCaprio_23
Species Specific ID: 7532
Target GenBank: NM_012479