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YWHAG (Homo sapiens) in pGEX

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Explanation of Terms


Gene: Gene Symbol:  YWHAG
Gene Name:  tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pGEX               Format:  CLOSED
Source: James DeCaprio
Description: None
Comments: seems that a pGEX-T4 vector was used (see PMID 9428519)
Mutation/
Discrepancy :
No / Unknown
Publications: PMID: 9428519
Title: The structural basis for 14-3-3:phosphopeptide binding specificity
PMID: 15107609
Title: 14-3-3 family members act coordinately to regulate mitotic progression
PMID: 10330186
Title: Cytoplasmic localization of human cdc25C during interphase requires an intact 14-3-3 binding site
Authors: James DeCaprio
Jocelyn Kasper
HsCD00017294

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: U Verification Method: 
Mutations:
None
Discrepancy:
None
Others:
Tag GST (from pGEX vector)
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pGEX

pGEX in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: pGEX-series GST fusion vector with Ptac promoter, lacI repressor; amp resistance; restriction enzyme cloning.
Comments: Map shown is for later versions of pGEX and may not be accurate for the original construct.
Size (bp): 4900
Parent Vector: None
Empty Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Amersham Pharmacia
Publications: None
Vector Map:         Vector Sequence:
Sequence: Not Available

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS BamHI, SmaI, EcoRI sites for in-frame cloning 0 0
bacterial origin ColE1 pBR322-type ColE1 origin 0 0
promoter Ptac Ptac promoter 0 0
selectable marker AmpR ampicillin resistance gene 0 0
tag GST glutathione S-transferase (GST) coding region 0 0

 

  • Gene
  • Pathways
  • Protein
  • Organism
  • Genome

Gene

 

Gene Symbol : YWHAG
Symbol Nomenclature : YWHAG
SYNONYM : 14-3-3GAMMA
Designation : 14-3-3 gamma|14-3-3 protein gamma|KCIP-1|protein kinase C inhibitor protein 1|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
Full Nomenclature : tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma
GENEID : 7532
SYNONYM : GST 14-3-3 g
SYNONYM : pGEX-YWHAG
SYNONYM : GST 14-4-4 gamma
SYNONYM : DeCaprio_23
HGNC : 12852
MIM : 605356
Vega : OTTHUMG00000022862
Target GenBank: NM_012479

 

Pathways

NCI : Class I PI3K signaling events mediated by Akt
NCI : ErbB1 downstream signaling
NCI : FoxO family signaling
NCI : IL3-mediated signaling events
NCI : Insulin-mediated glucose transport
NCI : LKB1 signaling events
NCI : PDGFR-beta signaling pathway
NCI : Regulation of nuclear beta catenin signaling and target gene transcription
NCI : Role of Calcineurin-dependent NFAT signaling in lymphocytes
NCI : Trk receptor signaling mediated by PI3K and PLC-gamma
NCI : a6b1 and a6b4 Integrin signaling
NCI : mTOR signaling pathway
NCI : p38 signaling mediated by MAPKAP kinases
Panther : EGF receptor signaling pathway
Panther : FGF signaling pathway
Panther : Parkinson disease
Reactome : Activation of BAD and translocation to mitochondria
Reactome : Activation of BH3-only proteins
Reactome : Apoptosis
Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Centrosome maturation
Reactome : G2/M Transition
Reactome : Intrinsic Pathway for Apoptosis
Reactome : Loss of Nlp from mitotic centrosomes
Reactome : Loss of proteins required for interphase microtubule organizationÿ from the centrosome
Reactome : Membrane Trafficking
Reactome : Mitotic G2-G2/M phases
Reactome : Recruitment of mitotic centrosome proteins and complexes
Reactome : Regulation of PLK1 Activity at G2/M Transition
Reactome : Translocation of GLUT4 to the plasma membrane
WikiPathway : Calcium Regulation in the Cardiac Cell
WikiPathway : Cell cycle
WikiPathway : Myometrial Relaxation and Contraction Pathways
WikiPathway : Prolactin Signaling Pathway

Protein

HPRD : 05639

Organism


TAX_ID : 9606
Species Specific ID: 7532

Genome

Chromosome : 7
Map Location : 7q11.23
Ensembl : ENSG00000170027