dnaE (V. cholerae O1 biovar El Tor str. N16961 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH174713.01F
Keyword:
None
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
VcCD00017461
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3495nts
Open reading frame: 1 to 3495

ATGGATACTCCTTGCATGTCAGACCCAAAGTTCATCCACTTACGTATACACAGTGATTTC
TCTATGGTGGATGGTTTGTCCAAAGTCCCGCCATTGGTGAAAAAAGTGGCGGCAATGGGC
ATGCCCGCAATGGCCTTGACCGATTTCACCAACCTGTGTGGCTTGGTGAAGTTTTACAGC
ACCGCGCATAACTGCGGGGTTAAGCCAATCATTGGCGCCGACTTTACTTTGCAATCGGAA
GAGTTCGGTGATGAGCTGACCAAACTGACCTTGCTTGCTAAAAACAATGTCGGTTACAAA
AACCTCACCTTATTGATTTCTAAAGCCTATTTACGCGGCCATGTCCAGCATCAACCTGTG
ATTGATAAAGCGTGGTTGGTCGAGCATGCCGAAGGGCTGATTGTGCTCTCGGGAGGCAAA
AGTGGCGAAGTGGGGCGCGCATTACTCAAAGGCAACCAACAACAAGTAGAAAGATGCATT
GAGTTTTACCAAACTCACTTTGCCGATCATTTCTATCTAGAGCTTATCCGCACTGGCCGT
GCTGATGAAGAGAGCTACTTACATTTTGCGCTGGATGTGGCAGAGCAGTACGACTTGCCC
GTCGTGGCAACCAACGAAGTGGTGTTCATCACTGAAGAGTCGTTTGAAGCCCACGAAATC
CGAGTCGCGATTCACGATGGTTATACGCTGGAAGATCCACGCCGTCCGAAAAATTACAGT
CCGAAGCAGTATCTGCGCAGCGAAGCGGAGATGTGTGAGCTGTTTGCAGACATTCCAGAA
GCGTTAGCCAACAGTGTCGAGATTGCCAAACGTTGTAACGTGACCGTACGTTTGGGCGAA
TATTTCCTACCTAACTTCCCAACGGGCGGGATGGCGATTGAAGATTTTCTGGTGATGAAA
TCTCGCGAAGGGTTGGAAGAGCGACTCGAATTTCTTTTCCCAGATCCAGAAGTTCGTGCC
AAGCGTCGCCCTGAATATGATGAGCGTTTGCAAGTTGAGCTCGATGTGATTAACCAGATG
GGCTTTCCCGGATACTTCTTGATCGTGATGGAGTTTATCCAGTGGTCAAAAGATAACGAT
ATTCCAGTCGGTCCGGGACGGGGCTCGGGGGCGGGCTCATTGGTAGCGTATGCACTGAAA
ATCACCGACCTTGATCCGCTGGAATACGATCTGCTGTTCGAGCGTTTCTTGAATCCAGAG
CGGGTCTCCATGCCCGACTTCGACGTCGATTTCTGTATGGATAAGCGTGATCAAGTAATT
GATCACGTAGCGGAAATGTATGGTCGTGATGCGGTATCACAAATCATCACTTTCGGTACC
ATGGCGGCGAAAGCGGTTATTCGCGATGTGGGGCGTGTACTTGGTCATCCGTTCGGTTTT
GTCGATCGCATTTCCAAATTAGTGCCACCCGATCCGGGTATGACGCTCGAGAAAGCCTTT
ATCGCCGAGCCTGCACTGCAAGAGCTGTATGACGCGGATGAGGAAGTCAAAGAGCTGATC
GACAAGTGTCGAATTCTGGAAGGCTGTACCCGTAACGCTGGTAAACACGCTGGGGGCGTG
GTGATTTCGCCCACTGCCATCACTGACTTTGCGCCGATTTACTGTGATGCGGAAGGCAAC
TTCCCGGTTACTCAGTTCGATAAGAACGATGTGGAAACTGCCGGTCTGGTCAAATTTGAC
TTCTTGGGGCTGCGTACCCTTACCATTATTGACTGGGCTTTGGGCTTAGTGAACCCGCGT
TTGAAAAAGGCGGGCAAACCCCCCGTGCGGATTGAAGCGATCCCGCTCGACGATGCGCGC
TCTTTCCGTAACCTGCAAGATGCCAAAACCACCGCGGTATTCCAGCTCGAATCGCGCGGT
ATGAAAGAGCTGATTAAGCGACTGCAACCCGACTGTTTTGAAGACATCATCGCTCTGGTG
GCGCTGTTTCGTCCGGGGCCGTTGCAATCCGGCATGGTGGATAACTTTATCGACCGTAAG
CATGGCCGAGAAGCCATCTCTTATCCGGATGAAAAGTGGCAACATGAATCGCTCAAAGAG
ATCCTAGAACCGACCTACGGCATCATCCTCTATCAGGAGCAGGTGATGCAGATCGCGCAG
GTACTGTCGGGTTACACCTTGGGTGGGGCAGACATGCTGCGCCGTGCAATGGGTAAGAAA
AAGCCAGAAGAAATGGCTAAGCAGCGCGCTGTGTTCCAAGAAGGGGCGGAGAAAAATGGC
GTCGATGGTGAGTTGGCGATGAAAATCTTCGATCTGGTGGAAAAATTCGCCGGATACGGA
TTTAACAAATCGCACTCTGCCGCTTATGCGCTGGTTTCCTACCAAACCTTATGGTTGAAA
ACCCACTATCCAGCCGAGTTTATGGCGGCGGTAATGACCGCGGATATGGACAACACGGAA
AAAGTGGTTGGCCTAGTGGATGAGTGTAAAAACATGGGATTGACCGTGTTGCCACCGGAC
ATTAACTCGGGTCTTTATCGCTTTAACGTCGATGACAACGGCGCGATTGTTTATGGTATT
GGCGCAATTAAAGGGGTGGGTGAAGGTCCGATTGAAGCGATTTTGGAAGCGCGCAACAAA
GGGGGATATTTCAAAGATCTGTTTGATTTCTGCGCGCGGATTGATCTGAAAAAGGTCAAC
AAACGGGTAATTGAAAAACTGATCTTGGCTGGCGCCCTGGATAGATTAGGCCCGCATCGC
GCAGCCATGATGGCCTCAGTGGATGATGCGGTGCGTGCAGCAAGCCAACATCACCAAGCG
GAAGCGTTTGGACAAGCGGATATGTTCGGTGTGCTCACGGATGCGCCAGAAGAAGTCGAG
CAGAAATACACGCAAGTGCCAGAATGGCCAGAAAAAGTGCGTTTGGAAGGGGAGCGCGAG
ACGCTTGGGCTCTATCTCACCGGCCATCCGGTGGATGAATACCTGAAAGAGCTCACCAAA
TACACCAGTTGCCGTTTGAATGAAGCCGCACCAACCCGGCGTGATCAATCCTTAACCGTT
GCCGGATTGGTGATTGCAGCGCGGGTGATGACCACCAAACGTGGCACGCGAATTGGTTTA
ATGACTTTAGATGACCGCTCGGGCCGTATGGAAGTGATGCTTTACTCGGAAGCACTCGAT
CGGTATGCAGAATGGTTAGAAAAAGATAAAATTCTGGTGGTTTCCGGACAGGTCAGCTTT
GATGACTTCAATGGCGGACTTAAAATGTCGGCGCGCGAGGTCATGGACTTGGGCAGCGCG
CGTGAAAAATTTGCCCGCGGTTTATCGATCTCGATTTTACAGTCGCAGATTGATCAACAA
TTTTTTGAGCGTTTTAGTCACATACTGGAACCTCACCGCGCAGGAACCGTACCTGTCAAT
GTATACTACCAGCGCCCTGATGCGCGAGCGCGCCTGACGTTAGGCACAGAGTGGCGAGTC
ACGCCAAGCGATACATTGTTAGACGAATTAAAGCAGCTACTTGGTCATGATCAGGTAGAA
CTCGAATTTAACTTG

Protein sequence predicted based on nucleotide sequence

MDTPCMSDPKFIHLRIHSDFSMVDGLSKVPPLVKKVAAMGMPAMALTDFTNLCGLVKFYS
TAHNCGVKPIIGADFTLQSEEFGDELTKLTLLAKNNVGYKNLTLLISKAYLRGHVQHQPV
IDKAWLVEHAEGLIVLSGGKSGEVGRALLKGNQQQVERCIEFYQTHFADHFYLELIRTGR
ADEESYLHFALDVAEQYDLPVVATNEVVFITEESFEAHEIRVAIHDGYTLEDPRRPKNYS
PKQYLRSEAEMCELFADIPEALANSVEIAKRCNVTVRLGEYFLPNFPTGGMAIEDFLVMK
SREGLEERLEFLFPDPEVRAKRRPEYDERLQVELDVINQMGFPGYFLIVMEFIQWSKDND
IPVGPGRGSGAGSLVAYALKITDLDPLEYDLLFERFLNPERVSMPDFDVDFCMDKRDQVI
DHVAEMYGRDAVSQIITFGTMAAKAVIRDVGRVLGHPFGFVDRISKLVPPDPGMTLEKAF
IAEPALQELYDADEEVKELIDKCRILEGCTRNAGKHAGGVVISPTAITDFAPIYCDAEGN
FPVTQFDKNDVETAGLVKFDFLGLRTLTIIDWALGLVNPRLKKAGKPPVRIEAIPLDDAR
SFRNLQDAKTTAVFQLESRGMKELIKRLQPDCFEDIIALVALFRPGPLQSGMVDNFIDRK
HGREAISYPDEKWQHESLKEILEPTYGIILYQEQVMQIAQVLSGYTLGGADMLRRAMGKK
KPEEMAKQRAVFQEGAEKNGVDGELAMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLK
THYPAEFMAAVMTADMDNTEKVVGLVDECKNMGLTVLPPDINSGLYRFNVDDNGAIVYGI
GAIKGVGEGPIEAILEARNKGGYFKDLFDFCARIDLKKVNKRVIEKLILAGALDRLGPHR
AAMMASVDDAVRAASQHHQAEAFGQADMFGVLTDAPEEVEQKYTQVPEWPEKVRLEGERE
TLGLYLTGHPVDEYLKELTKYTSCRLNEAAPTRRDQSLTVAGLVIAARVMTTKRGTRIGL
MTLDDRSGRMEVMLYSEALDRYAEWLEKDKILVVSGQVSFDDFNGGLKMSAREVMDLGSA
REKFARGLSISILQSQIDQQFFERFSHILEPHRAGTVPVNVYYQRPDARARLTLGTEWRV
TPSDTLLDELKQLLGHDQVELEFNL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3495
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable