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STK17A (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  STK17A
Gene Name:  serine/threonine kinase 17a
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH000141.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors: HIP
HsCD00000178

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1245nts         Open reading frame : 1 to 1245
Reference Sequence Alignment

ATGATCCCTTTGGAGAAGCCAGGCAGCGGCGGCTCCTCCCCAGGCGCCACCTCAGGCTCG
GGCCGGGCAGGCCGGGGTCTGAGCGGGCCGTGCCGGCCGCCGCCGCCGCCCCAGGCCCGC
GGGCTGCTGACAGAGATACGCGCCGTGGTGCGCACCGAGCCCTTCCAGGACGGCTACAGC
CTGTGCCCGGGCCGGGAGCTGGGCAGGGGGAAATTTGCAGTGGTGAGAAAATGTATAAAG
AAAGATTCTGGGAAAGAATTTGCTGCAAAGTTCATGAGAAAAAGAAGAAAAGGCCAAGAT
TGTCGGATGGAAATAATTCATGAGATTGCTGTACTTGAACTAGCACAAGACAATCCTTGG
GTCATTAATTTACATGAAGTTTATGAGACTGCATCAGAAATGATCTTAGTTCTGGAATAT
GCTGCTGGGGGTGAAATCTTTGACCAGTGTGTTGCAGACAGAGAAGAAGCCTTTAAAGAA
AAAGATGTTCAAAGACTTATGCGACAGATTTTAGAAGGTGTTCACTTTTTACACACTCGT
GATGTAGTTCATCTTGATTTGAAGCCTCAGAATATTCTGTTGACAAGTGAATCTCCATTG
GGTGACATTAAGATTGTTGATTTTGGCCTTTCAAGAATATTGAAGAACAGTGAAGAGCTC
CGAGAAATTATGGGTACCCCTGAATATGTGGCTCCTGAAATTCTTAGTTATGATCCTATA
AGCATGGCAACAGATATGTGGAGCATTGGAGTGTTAACATATGTCATGCTTACAGGAATA
TCACCTTTCTTAGGCAATGATAAACAAGAAACATTCTTAAACATCTCACAGATGAATTTA
AGTTATTCTGAGGAAGAATTTGATGTTTTGTCTGAGTCGGCTGTTGATTTCATCAGGACA
CTTTTAGTTAAGAAACCTGAAGATCGAGCCACTGCTGAAGAATGTCTAAAGCACCCCTGG
TTGACACAGAGCAGTATTCAAGAGCCTTCTTTCAGGATGGAAAAGGCACTAGAAGAAGCA
AATGCCCTCCAAGAAGGTCATTCTGTGCCTGAAATTAATTCGGATACCGACAAATCAGAA
ACCGAGGAATCCATTGTAACCGAAGAGTTAATTGTAGTTACTTCATATACTCTAGGACAA
TGCAGACAGTCTGAAAAAGAGAAAATGGAGCAAAAGGCCATTTCCAAACGATTTAAATTT
GAGGAACCTTTGCTACAAGAAATTCCAGGAGAATTTATCTACTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1362
Start on reference sequence 118
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : STK17A
Symbol Nomenclature : STK17A
SYNONYM : DRAK1
Designation : DAP kinase-related apoptosis-inducing protein kinase 1|death-associated protein kinase-related 1|serine/threonine kinase 17a (apoptosis-inducing)|serine/threonine-protein kinase 17A
Full Nomenclature : serine/threonine kinase 17a
GENEID : 9263
GI : 33304032
GenBank Accession : AY335697
HGNC : 11395
MIM : 604726
Vega : OTTHUMG00000022825
Target GenBank: NM_004760

 

Reference Sequence Alignment

  

HsCD00000178      ATGATCCCTTTGGAGAAGCCAGGCAGCGGCGGCTCCTCCCCAGGCGCCACCTCAGGCTCG
NM_004760.2       ATGATCCCTTTGGAGAAGCCAGGCAGCGGCGGCTCCTCCCCAGGCGCCACCTCAGGCTCG
                  ************************************************************

HsCD00000178      GGCCGGGCAGGCCGGGGTCTGAGCGGGCCGTGCCGGCCGCCGCCGCCGCCCCAGGCCCGC
NM_004760.2       GGCCGGGCAGGCCGGGGTCTGAGCGGGCCGTGCCGGCCGCCGCCGCCGCCCCAGGCCCGC
                  ************************************************************

HsCD00000178      GGGCTGCTGACAGAGATACGCGCCGTGGTGCGCACCGAGCCCTTCCAGGACGGCTACAGC
NM_004760.2       GGGCTGCTGACAGAGATACGCGCCGTGGTGCGCACCGAGCCCTTCCAGGACGGCTACAGC
                  ************************************************************

HsCD00000178      CTGTGCCCGGGCCGGGAGCTGGGCAGGGGGAAATTTGCAGTGGTGAGAAAATGTATAAAG
NM_004760.2       CTGTGCCCGGGCCGGGAGCTGGGCAGGGGGAAATTTGCAGTGGTGAGAAAATGTATAAAG
                  ************************************************************

HsCD00000178      AAAGATTCTGGGAAAGAATTTGCTGCAAAGTTCATGAGAAAAAGAAGAAAAGGCCAAGAT
NM_004760.2       AAAGATTCTGGGAAAGAATTTGCTGCAAAGTTCATGAGAAAAAGAAGAAAAGGCCAAGAT
                  ************************************************************

HsCD00000178      TGTCGGATGGAAATAATTCATGAGATTGCTGTACTTGAACTAGCACAAGACAATCCTTGG
NM_004760.2       TGTCGGATGGAAATAATTCATGAGATTGCTGTACTTGAACTAGCACAAGACAATCCTTGG
                  ************************************************************

HsCD00000178      GTCATTAATTTACATGAAGTTTATGAGACTGCATCAGAAATGATCTTAGTTCTGGAATAT
NM_004760.2       GTCATTAATTTACATGAAGTTTATGAGACTGCATCAGAAATGATCTTAGTTCTGGAATAT
                  ************************************************************

HsCD00000178      GCTGCTGGGGGTGAAATCTTTGACCAGTGTGTTGCAGACAGAGAAGAAGCCTTTAAAGAA
NM_004760.2       GCTGCTGGGGGTGAAATCTTTGACCAGTGTGTTGCAGACAGAGAAGAAGCCTTTAAAGAA
                  ************************************************************

HsCD00000178      AAAGATGTTCAAAGACTTATGCGACAGATTTTAGAAGGTGTTCACTTTTTACACACTCGT
NM_004760.2       AAAGATGTTCAAAGACTTATGCGACAGATTTTAGAAGGTGTTCACTTTTTACACACTCGT
                  ************************************************************

HsCD00000178      GATGTAGTTCATCTTGATTTGAAGCCTCAGAATATTCTGTTGACAAGTGAATCTCCATTG
NM_004760.2       GATGTAGTTCATCTTGATTTGAAGCCTCAGAATATTCTGTTGACAAGTGAATCTCCATTG
                  ************************************************************

HsCD00000178      GGTGACATTAAGATTGTTGATTTTGGCCTTTCAAGAATATTGAAGAACAGTGAAGAGCTC
NM_004760.2       GGTGACATTAAGATTGTTGATTTTGGCCTTTCAAGAATATTGAAGAACAGTGAAGAGCTC
                  ************************************************************

HsCD00000178      CGAGAAATTATGGGTACCCCTGAATATGTGGCTCCTGAAATTCTTAGTTATGATCCTATA
NM_004760.2       CGAGAAATTATGGGTACCCCTGAATATGTGGCTCCTGAAATTCTTAGTTATGATCCTATA
                  ************************************************************

HsCD00000178      AGCATGGCAACAGATATGTGGAGCATTGGAGTGTTAACATATGTCATGCTTACAGGAATA
NM_004760.2       AGCATGGCAACAGATATGTGGAGCATTGGAGTGTTAACATATGTCATGCTTACAGGAATA
                  ************************************************************

HsCD00000178      TCACCTTTCTTAGGCAATGATAAACAAGAAACATTCTTAAACATCTCACAGATGAATTTA
NM_004760.2       TCACCTTTCTTAGGCAATGATAAACAAGAAACATTCTTAAACATCTCACAGATGAATTTA
                  ************************************************************

HsCD00000178      AGTTATTCTGAGGAAGAATTTGATGTTTTGTCTGAGTCGGCTGTTGATTTCATCAGGACA
NM_004760.2       AGTTATTCTGAGGAAGAATTTGATGTTTTGTCTGAGTCGGCTGTTGATTTCATCAGGACA
                  ************************************************************

HsCD00000178      CTTTTAGTTAAGAAACCTGAAGATCGAGCCACTGCTGAAGAATGTCTAAAGCACCCCTGG
NM_004760.2       CTTTTAGTTAAGAAACCTGAAGATCGAGCCACTGCTGAAGAATGTCTAAAGCACCCCTGG
                  ************************************************************

HsCD00000178      TTGACACAGAGCAGTATTCAAGAGCCTTCTTTCAGGATGGAAAAGGCACTAGAAGAAGCA
NM_004760.2       TTGACACAGAGCAGTATTCAAGAGCCTTCTTTCAGGATGGAAAAGGCACTAGAAGAAGCA
                  ************************************************************

HsCD00000178      AATGCCCTCCAAGAAGGTCATTCTGTGCCTGAAATTAATTCGGATACCGACAAATCAGAA
NM_004760.2       AATGCCCTCCAAGAAGGTCATTCTGTGCCTGAAATTAATTCGGATACCGACAAATCAGAA
                  ************************************************************

HsCD00000178      ACCGAGGAATCCATTGTAACCGAAGAGTTAATTGTAGTTACTTCATATACTCTAGGACAA
NM_004760.2       ACCAAGGAATCCATTGTAACCGAAGAGTTAATTGTAGTTACTTCATATACTCTAGGACAA
                  ***.********************************************************

HsCD00000178      TGCAGACAGTCTGAAAAAGAGAAAATGGAGCAAAAGGCCATTTCCAAACGATTTAAATTT
NM_004760.2       TGCAGACAGTCTGAAAAAGAGAAAATGGAGCAAAAGGCCATTTCCAAACGATTTAAATTT
                  ************************************************************

HsCD00000178      GAGGAACCTTTGCTACAAGAAATTCCAGGAGAATTTATCTACTTG
NM_004760.2       GAGGAACCTTTGCTACAAGAAATTCCAGGAGAATTTATCTACTGA
                  ******************************************* .

Clone

Cloning Information : 141
HIP Master Clone ID : 87199
Original Clone ID : FLH000141.01L

Organism


TAX_ID : 9606
Species Specific ID: 9263

Genome

Chromosome : 7
Map Location : 7p13
Ensembl : ENSG00000164543

Reagents

Labome : STK17A-antibody