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SNX1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  SNX1
Gene Name:  sorting nexin 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH026203.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00001807

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1569nts         Open reading frame : 1 to 1569
Reference Sequence Alignment

ATGGCGTCGGGTGGTGGTGGCTGTAGCGCTTCGGAGAGACTGCCTCCGCCCTTCCCCGGC
CTGGAGCCGGAGTCCGAGGGGGCGGCCGGGGGATCAGAACCCGAGGCTGGGGACAGCGAC
ACCGAGGGGGAGGACATTTTCACCGGCGCCGCGGTGGTCAGTAAACATCAGTCTCCAAAG
ATAACTACATCCCTTCTTCCCATCAACAATGGCTCCAAAGAAAATGGGATCCATGAAGAA
CAAGACCAAGAGCCACAGGATCTCTTTGCAGATGCCACAGTGGAGCTATCCTTGGACAGC
ACACAAAATAATCAGAAGAAGGTGCTAGCCAAAACACTCATTTCTCTTCCTCCTCAGGAA
GCCACAAATTCTTCGAAGCCCCAGCCAACCTATGAGGAGCTAGAGGAAGAAGAACAGGAG
GATCAATTTGATTTGACAGTCGGTATAACTGATCCTGAGAAGATAGGGGATGGTATGAAT
GCATATGTAGCCTACAAAGTTACAACACAGACAAGCTTACCATTGTTCAGAAGCAAACAG
TTTGCAGTAAAAAGAAGATTTAGTGACTTTCTGGGTCTTTATGAGAAGCTTTCCGAGAAG
CACTCTCAGAATGGCTTCATTGTCCCTCCACCCCCGGAGAAGAGCCTCATAGGGATGACA
AAAGTGAAAGTTGGGAAGGAAGATTCTTCTTCTGCAGAATTTCTTGAAAAACGGAGGGCC
GCTTTAGAAAGGTACCTTCAGAGGATTGTAAATCATCCTACCATGTTACAGGACCCTGAC
GTCAGAGAGTTCTTGGAAAAAGAAGAGCTGCCACGTGCCGTGGGTACCCAGACATTGAGT
GGTGCTGGTCTCCTCAAGATGTTCAACAAAGCCACAGATGCCGTCAGCAAAATGACCATC
AAGATGAATGAATCAGACATTTGGTTTGAGGAGAAGCTCCAGGAGGTAGAGTGTGAGGAG
CAGCGCTTACGGAAACTGCATGCTGTTGTAGAAACTCTAGTCAACCATAGGAAAGAGCTA
GCGCTGAACACAGCCCAGTTTGCAAAGAGTCTAGCCATGCTTGGGAGCTCTGAGGACAAC
ACGGCATTGTCACGGGCACTCTCCCAGCTGGCTGAGGTGGAAGAAAAAATTGAGCAGCTC
CACCAGGAACAGGCCAACAATGACTTCTTCCTCCTTGCTGAGCTCCTGAGTGACTACATT
CGCCTCCTGGCCATAGTCCGCGCTGCCTTCGACCAGCGCATGAAGACATGGCAGCGCTGG
CAGGATGCCCAAGCCACACTGCAGAAGAAGCGGGAGGCCGAGGCTCGGCTGCTGTGGGCC
AACAAGCCTGATAAGCTGCAGCAGGCCAAGGACGAGATCCTCGAGTGGGAGTCTCGGGTG
ACTCAATATGAAAGGGACTTCGAGAGGATTTCAACAGTGGTCCGAAAAGAAGTGATACGG
TTTGAGAAAGAGAAATCCAAGGACTTCAAGAACCACGTGATCAAGTACCTTGAGACACTC
CTTTACTCACAGCAGCAGCTGGCAAAGTACTGGGAAGCCTTCCTTCCTGAGGCAAAGGCC
ATCTCCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1588
Start on reference sequence 20
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SNX1
Symbol Nomenclature : SNX1
SYNONYM : HsT17379; VPS5
Designation : sorting nexin 1A|sorting nexin-1
Full Nomenclature : sorting nexin 1
GENEID : 6642
GI : 61369356
GenBank Accession : AY891395
HGNC : 11172
MIM : 601272
Target GenBank: BC000357

 

Reference Sequence Alignment

  

HsCD00001807        ATGGCGTCGGGTGGTGGTGGCTGTAGCGCTTCGGAGAGACTGCCTCCGCCCTTCCCCGGC
NM_001242933.1      ATGGCGTCGGGTGGTGGTGGCTGTAGCGCTTCGGAGAGACTGCCTCCGCCCTTCCCCGGC
NM_003099.4         ATGGCGTCGGGTGGTGGTGGCTGTAGCGCTTCGGAGAGACTGCCTCCGCCCTTCCCCGGC
                    ************************************************************

HsCD00001807        CTGGAGCCGGAGTCCGAGGGGGCGGCCGGGGGATCAGAACCCGAGGCTGGGGACAGCGAC
NM_001242933.1      CTGGAGCCGGAGTCCGAGGGGGCGGCCGGGGGATCAGAACCCGAGGCTGGGGACAGCGAC
NM_003099.4         CTGGAGCCGGAGTCCGAGGGGGCGGCCGGGGGATCAGAACCCGAGGCTGGGGACAGCGAC
                    ************************************************************

HsCD00001807        ACCGAGGGGGAGGACATTTTCACCGGCGCCGCGGTGGTCAGTAAACATCAGTCTCCAAAG
NM_001242933.1      ACCGAGGGGGAGGACATTTTCACCGGCGCCGCGGTGGTCAGTAAACATCAGTCTCCAAAG
NM_003099.4         ACCGAGGGGGAGGACATTTTCACCGGCGCCGCGGTGGTCAGTAAACATCAGTCTCCAAAG
                    ************************************************************

HsCD00001807        ATAACTACATCCCTTCTTCCCATCAACAATGGCTCCAAAGAAAATGGGATCCATGAAGAA
NM_001242933.1      ATAACTACATCCCTTCTTCCCATCAACAATGGCTCCAAAGAAAATGGGATCCATGAAGAA
NM_003099.4         ATAACTACATCCCTTCTTCCCATCAACAATGGCTCCAAAGAAAATGGGATCCATGAAGAA
                    ************************************************************

HsCD00001807        CAAGACCAAGAGCCACAGGATCTCTTTGCAGATGCCACAGTGGAGCTATCCTTGGACAGC
NM_001242933.1      CAAGACCAAGAGCCACAGGATCTCTTTGCAGATGCCACAGTGGAGCTATCCTTGGACAGC
NM_003099.4         CAAGACCAAGAGCCACAGGATCTCTTTGCAGATGCCACAGTGGAGCTATCCTTGGACAGC
                    ************************************************************

HsCD00001807        ACACAAAATAATCAGAAGAAGGTGCTAGCCAAAACACTCATTTCTCTTCCTCCTCAGGAA
NM_001242933.1      ACACAAAATAATCAGAAGAAGGTGCTAGCCAAAACACTCATTTCTCTTCCTCCTCAGGAA
NM_003099.4         ACACAAAATAATCAGAAGAAGGTGCTAGCCAAAACACTCATTTCTCTTCCTCCTCAGGAA
                    ************************************************************

HsCD00001807        GCCACAAATTCTTCGAAGCCCCAGCCAACCTATGAGGAGCTAGAGGAAGAAGAACAGGAG
NM_001242933.1      GCCACAAATTCTTCGAAGCCCCAGCCAACCTATGAGGAGCTAGAGGAAGAAGAACAGGAG
NM_003099.4         GCCACAAATTCTTCGAAGCCCCAGCCAACCTATGAGGAGCTAGAGGAAGAAGAACAGGAG
                    ************************************************************

HsCD00001807        GATCAATTTGATTTGACAGTCGGTATAACTGATCCTGAGAAGATAGGGGATGGTATGAAT
NM_001242933.1      GATCAATTTGATTTGACAGTCGGTATAACTGATCCTGAGAAGATAGGGGATGGTATGAAT
NM_003099.4         GATCAATTTGATTTGACAGTCGGTATAACTGATCCTGAGAAGATAGGGGATGGTATGAAT
                    ************************************************************

HsCD00001807        GCATATGTAGCCTACAAAGTTACAACACAGACAAGCTTACCATTGTTCAGAAGCAAACAG
NM_001242933.1      GCATATGTAGCCTACAAAGTTACAACACAGACAAGCTTACCATTGTTCAGAAGCAAACAG
NM_003099.4         GCATATGTAGCCTACAAAGTTACAACACAGACAAGCTTACCATTGTTCAGAAGCAAACAG
                    ************************************************************

HsCD00001807        TTTGCAGTAAAAAGAAGATTTAGTGACTTTCTGGGTCTTTATGAGAAGCTTTCCGAGAAG
NM_001242933.1      TTTGCAGTAAAAAGAAGATTTAGTGACTTTCTGGGTCTTTATGAGAAGCTTTCCGAGAAG
NM_003099.4         TTTGCAGTAAAAAGAAGATTTAGTGACTTTCTGGGTCTTTATGAGAAGCTTTCCGAGAAG
                    ************************************************************

HsCD00001807        CACTCTCAGAATGGCTTCATTGTCCCTCCACCCCCGGAGAAGAGCCTCATAGGGATGACA
NM_001242933.1      CACTCTCAGAATGGCTTCATTGTCCCTCCGCCCCCGGAGAAGAGCCTCATAGGGATGACA
NM_003099.4         CACTCTCAGAATGGCTTCATTGTCCCTCCGCCCCCGGAGAAGAGCCTCATAGGGATGACA
                    *****************************.******************************

HsCD00001807        AAAGTGAAAGTTGGGAAGGAAGATTCTTCTTCTGCAGAATTTCTTGAAAAACGGAGGGCC
NM_001242933.1      AAAGTGAAAGTTGGGAAGGAAGATTCTTCTTCTGCAGAATTTCTTGAAAAACGGAGGGCC
NM_003099.4         AAAGTGAAAGTTGGGAAGGAAGATTCTTCTTCTGCAGAATTTCTTGAAAAACGGAGGGCC
                    ************************************************************

HsCD00001807        GCTTTAGAAAGGTACCTTCAGAGGATTGTAAATCATCCTACCATGTTACAGGACCCTGAC
NM_001242933.1      GCTTTAGAAAGGTACCTTCAGAGGATTGTAAATCATCCTACCATGTTACAGGACCCTGAC
NM_003099.4         GCTTTAGAAAGGTACCTTCAGAGGATTGTAAATCATCCTACCATGTTACAGGACCCTGAC
                    ************************************************************

HsCD00001807        GTCAGAGAGTTCTTGGAAAAAGAAGAGCTGCCACGTGCCGTGGGTACCCAGACATTGAGT
NM_001242933.1      GTCAGAGAGTTCTTGGAAAAAGAAGAGCTGCCACGTGCCGTGGGTACCCAGACATTGAGT
NM_003099.4         GTCAGAGAGTTCTTGGAAAAAGAAGAGCTGCCACGTGCCGTGGGTACCCAGACATTGAGT
                    ************************************************************

HsCD00001807        GGTGCTGGTCTCCTCAAGATGTTCAACAAAGCCACAGATGCCGTCAGCAAAATGACCATC
NM_001242933.1      GGTGCTGGTCTCCTCAAGATGTTCAACAAAGCCACAGATGCCGTCAGCAAAATGACCATC
NM_003099.4         GGTGCTGGTCTCCTCAAGATGTTCAACAAAGCCACAGATGCCGTCAGCAAAATGACCATC
                    ************************************************************

HsCD00001807        AAGATGAATGAATCAGACATTTGGTTTGAGGAGAAGCTCCAGGAGGTAGAGTGTGAGGAG
NM_001242933.1      AAGATGAATGAATCAGACATTTGGTTTGAGGAGAAGCTCCAGGAGGTAGAGTGTGAGGAG
NM_003099.4         AAGATGAATGAATCAGACATTTGGTTTGAGGAGAAGCTCCAGGAGGTAGAGTGTGAGGAG
                    ************************************************************

HsCD00001807        CAGCGCTTACGGAAACTGCATGCTGTTGTAGAAACTCTAGTCAACCATAGGAAAGAGCTA
NM_001242933.1      CAGCGCTTACGGAAACTGCATGCTGTTGTAGAAACTCTAGTCAACCATAGGAAAGAGCTA
NM_003099.4         CAGCGCTTACGGAAACTGCATGCTGTTGTAGAAACTCTAGTCAACCATAGGAAAGAGCTA
                    ************************************************************

HsCD00001807        GCGCTGAACACAGCCCAGTTTGCAAAGAGTCTAGCCATGCTTGGGAGCTCTGAGGACAAC
NM_001242933.1      GCGCTGAACACAGCCCAGTTTGCAAAGAGTCTAGCCATGCTTGGGAGCTCTGAGGACAAC
NM_003099.4         GCGCTGAACACAGCCCAGTTTGCAAAGAGTCTAGCCATGCTTGGGAGCTCTGAGGACAAC
                    ************************************************************

HsCD00001807        ACGGCATTGTCACGGGCACTCTCCCAGCTGGCTGAGGTGGAAGAAAAAATTGAGCAGCTC
NM_001242933.1      ACGGCATTGTCACGGGCACTCTCCCAGCTGGCTGAGGTGGAAGAAAAAATTGAGCAGCTC
NM_003099.4         ACGGCATTGTCACGGGCACTCTCCCAGCTGGCTGAGGTGGAAGAAAAAATTGAGCAGCTC
                    ************************************************************

HsCD00001807        CACCAGGAACAGGCCAACAATGACTTCTTCCTCCTTGCTGAGCTCCTGAGTGACTACATT
NM_001242933.1      CACCAGGAACAGGCCAACAATGACTTCTTCCTCCTTGCTGAGCTCCTGAGTGACTACATT
NM_003099.4         CACCAGGAACAGGCCAACAATGACTTCTTCCTCCTTGCTGAGCTCCTGAGTGACTACATT
                    ************************************************************

HsCD00001807        CGCCTCCTGGCCATAGTCCGCGCTGCCTTCGACCAGCGCATGAAGACATGGCAGCGCTGG
NM_001242933.1      CGCCTCCTGGCCATAGTCCGCGCTGCCTTCGACCAGCGCATGAAGACATGGCAGCGCTGG
NM_003099.4         CGCCTCCTGGCCATAGTCCGCGCTGCCTTCGACCAGCGCATGAAGACATGGCAGCGCTGG
                    ************************************************************

HsCD00001807        CAGGATGCCCAAGCCACACTGCAGAAGAAGCGGGAGGCCGAGGCTCGGCTGCTGTGGGCC
NM_001242933.1      CAGGATGCCCAAGCCACACTGCAGAAGAAGCGGGAGGCCGAGGCTCGGCTGCTGTGGGCC
NM_003099.4         CAGGATGCCCAAGCCACACTGCAGAAGAAGCGGGAGGCCGAGGCTCGGCTGCTGTGGGCC
                    ************************************************************

HsCD00001807        AACAAGCCTGATAAGCTGCAGCAGGCCAAGGACGAGATCCTCGAGTGGGAGTCTCGGGTG
NM_001242933.1      AACAAGCCTGATAAGCTGCAGCAGGCCAAGGACGAGATCCTCGAGTGGGAGTCTCGGGTG
NM_003099.4         AACAAGCCTGATAAGCTGCAGCAGGCCAAGGACGAGATCCTCGAGTGGGAGTCTCGGGTG
                    ************************************************************

HsCD00001807        ACTCAATATGAAAGGGACTTCGAGAGGATTTCAACAGTGGTCCGAAAAGAAGTGATACGG
NM_001242933.1      ACTCAATATGAAAGGGACTTCGAGAGGATTTCAACAGTGGTCCGAAAAGAAGTGATACGG
NM_003099.4         ACTCAATATGAAAGGGACTTCGAGAGGATTTCAACAGTGGTCCGAAAAGAAGTGATACGG
                    ************************************************************

HsCD00001807        TTTGAGAAAGAGAAATCCAAGGACTTCAAGAACCACGTGATCAAGTACCTTGAGACACTC
NM_001242933.1      TTTGAGAAAGAGAAATCCAAGGACTTCAAGAACCACGTGATCAAGTACCTTGAGACACTC
NM_003099.4         TTTGAGAAAGAGAAATCCAAGGACTTCAAGAACCACGTGATCAAGTACCTTGAGACACTC
                    ************************************************************

HsCD00001807        CTTTACTCACAGCAGCAGCTGGCAAAGTACTGGGAAGCCTTCCTTCCTGAGGCAAAGGCC
NM_001242933.1      CTTTACTCACAGCAGCAGGCTGGGGAGCAGTTGGGAATCAGGTCTGGAATACTCCTAACC
NM_003099.4         CTTTACTCACAGCAGCAGCTGGCAAAGTACTGGGAAGCCTTCCTTCCTGAGGCAAAGGCC
                    ******************   * ..** * * **.*. *:    *  :.:.  ..:..**

HsCD00001807        ATCTCCTTG---------------------------------------------------
NM_001242933.1      AAGAAGTTGCCCAGGTATAGTAAGTTTTTCTCTACCGTTCACAAGTTTTGTGCTGCTGCT
NM_003099.4         ATCTCCTAA---------------------------------------------------
                    *: :. *:.                                                   

HsCD00001807        ------------------------------------------------------
NM_001242933.1      TCCCTCTGGAAATGGGGTTTCTTTCTCTCCGCCTACCTCAGCTACCTGTTCTGA
NM_003099.4         ------------------------------------------------------
                                                                          

Pathways

NCI : PAR1-mediated thrombin signaling events
NCI : Posttranslational regulation of adherens junction stability and dissassembly

Protein

SMART domain : PX : PhoX homologous domain, present in p47phox and p40phox.
UniProt : Q13596
UniProt : A6NKH4
HPRD : 03171

Clone

Cloning Information : 26203
HIP Master Clone ID : 56579
Original Clone ID : FLH026203.01L

Organism


TAX_ID : 9606
Species Specific ID: 6642

Genome

Chromosome : 15
Map Location : 15q22.31
Ensembl : ENSG00000028528

Reagents

Labome : SNX1-antibody