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RPS6 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  RPS6
Gene Name:  ribosomal protein S6
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH030768.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00001811

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 750nts         Open reading frame : 1 to 750
Reference Sequence Alignment

ATGAAGCTGAACATCTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACGAT
GAACGCAAACTTCGTACTTTCTATGAGAAGCGTATGGCCACAGAAGTTGCTGCTGACGCT
CTGGGTGAAGAATGGAAGGGTTATGTGGTCCGAATCAGTGGTGGGAACGACAAACAAGGT
TTCCCCATGAAGCAGGGTGTCTTGACCCATGGCCGTGTCCGCCTGCTACTGAGTAAGGGG
CATTCCTGTTACAGACCAAGGAGAACTGGAGAAAGAAAGAGAAAATCAGTTCGTGGTTGC
ATTGTGGATGCAAATCTGAGCGTTCTCAACTTGGTTATTGTAAAAAAAGGAGAGAAGGAT
ATTCCTGGACTGACTGATACTACAGTGCCTCGCCGCCTGGGCCCCAAAAGAGCTAGCAGA
ATCCGCAAACTTTTCAATCTCTCTAAAGAAGATGATGTCCGCCAGTATGTTGTAAGAAAG
CCCTTAAATAAAGAAGGTAAGAAACCTAGGACCAAAGCACCCAAGATTCAGCGTCTTGTT
ACTCCACGTGTCCTGCAGCACAAACGGCGGCGTATTGCTCTGAAGAAGCAGCGTACCAAG
AAAAATAAAGAAGAGGCTGCAGAATATGCTAAACTTTTGGCCAAGAGAATGAAGGAGGCT
AAGGAGAAGCGCCAGGAACAAATTGCGAAGAGACGCAGACTTTCCTCTCTGCGAGCTTCT
ACTTCTAAGTCTGAATCCAGTCAGAAATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 763
Start on reference sequence 14
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RPS6
Symbol Nomenclature : RPS6
SYNONYM : S6
Designation : 40S ribosomal protein S6|phosphoprotein NP33
Full Nomenclature : ribosomal protein S6
GENEID : 6194
Locus Tag : RP11-513M16.6
GI : 61359218
GenBank Accession : AY888743
HGNC : 10429
MIM : 180460
Vega : OTTHUMG00000019642
Target GenBank: BC000524

 

Reference Sequence Alignment

  

HsCD00001811      ATGAAGCTGAACATCTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACGAT
NM_001010.2       ATGAAGCTGAACATCTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACGAT
                  ************************************************************

HsCD00001811      GAACGCAAACTTCGTACTTTCTATGAGAAGCGTATGGCCACAGAAGTTGCTGCTGACGCT
NM_001010.2       GAACGCAAACTTCGTACTTTCTATGAGAAGCGTATGGCCACAGAAGTTGCTGCTGACGCT
                  ************************************************************

HsCD00001811      CTGGGTGAAGAATGGAAGGGTTATGTGGTCCGAATCAGTGGTGGGAACGACAAACAAGGT
NM_001010.2       CTGGGTGAAGAATGGAAGGGTTATGTGGTCCGAATCAGTGGTGGGAACGACAAACAAGGT
                  ************************************************************

HsCD00001811      TTCCCCATGAAGCAGGGTGTCTTGACCCATGGCCGTGTCCGCCTGCTACTGAGTAAGGGG
NM_001010.2       TTCCCCATGAAGCAGGGTGTCTTGACCCATGGCCGTGTCCGCCTGCTACTGAGTAAGGGG
                  ************************************************************

HsCD00001811      CATTCCTGTTACAGACCAAGGAGAACTGGAGAAAGAAAGAGAAAATCAGTTCGTGGTTGC
NM_001010.2       CATTCCTGTTACAGACCAAGGAGAACTGGAGAAAGAAAGAGAAAATCAGTTCGTGGTTGC
                  ************************************************************

HsCD00001811      ATTGTGGATGCAAATCTGAGCGTTCTCAACTTGGTTATTGTAAAAAAAGGAGAGAAGGAT
NM_001010.2       ATTGTGGATGCAAATCTGAGCGTTCTCAACTTGGTTATTGTAAAAAAAGGAGAGAAGGAT
                  ************************************************************

HsCD00001811      ATTCCTGGACTGACTGATACTACAGTGCCTCGCCGCCTGGGCCCCAAAAGAGCTAGCAGA
NM_001010.2       ATTCCTGGACTGACTGATACTACAGTGCCTCGCCGCCTGGGCCCCAAAAGAGCTAGCAGA
                  ************************************************************

HsCD00001811      ATCCGCAAACTTTTCAATCTCTCTAAAGAAGATGATGTCCGCCAGTATGTTGTAAGAAAG
NM_001010.2       ATCCGCAAACTTTTCAATCTCTCTAAAGAAGATGATGTCCGCCAGTATGTTGTAAGAAAG
                  ************************************************************

HsCD00001811      CCCTTAAATAAAGAAGGTAAGAAACCTAGGACCAAAGCACCCAAGATTCAGCGTCTTGTT
NM_001010.2       CCCTTAAATAAAGAAGGTAAGAAACCTAGGACCAAAGCACCCAAGATTCAGCGTCTTGTT
                  ************************************************************

HsCD00001811      ACTCCACGTGTCCTGCAGCACAAACGGCGGCGTATTGCTCTGAAGAAGCAGCGTACCAAG
NM_001010.2       ACTCCACGTGTCCTGCAGCACAAACGGCGGCGTATTGCTCTGAAGAAGCAGCGTACCAAG
                  ************************************************************

HsCD00001811      AAAAATAAAGAAGAGGCTGCAGAATATGCTAAACTTTTGGCCAAGAGAATGAAGGAGGCT
NM_001010.2       AAAAATAAAGAAGAGGCTGCAGAATATGCTAAACTTTTGGCCAAGAGAATGAAGGAGGCT
                  ************************************************************

HsCD00001811      AAGGAGAAGCGCCAGGAACAAATTGCGAAGAGACGCAGACTTTCCTCTCTGCGAGCTTCT
NM_001010.2       AAGGAGAAGCGCCAGGAACAAATTGCGAAGAGACGCAGACTTTCCTCTCTGCGAGCTTCT
                  ************************************************************

HsCD00001811      ACTTCTAAGTCTGAATCCAGTCAGAAATAA
NM_001010.2       ACTTCTAAGTCTGAATCCAGTCAGAAATAA
                  ******************************

Pathways

NCI : ErbB1 downstream signaling
NCI : IL2 signaling events mediated by PI3K
Reactome : 3' -UTR-mediated translational regulation
Reactome : Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
Reactome : Cap-dependent Translation Initiation
Reactome : Disease
Reactome : Eukaryotic Translation Elongation
Reactome : Eukaryotic Translation Initiation
Reactome : Eukaryotic Translation Termination
Reactome : Formation of a pool of free 40S subunits
Reactome : Formation of the ternary complex, and subsequently, the 43S complex
Reactome : GTP hydrolysis and joining of the 60S ribosomal subunit
Reactome : Gene Expression
Reactome : IGF1R signaling cascade
Reactome : IRS-mediated signalling
Reactome : IRS-related events
Reactome : IRS-related events triggered by IGF1R
Reactome : Influenza Infection
Reactome : Influenza Life Cycle
Reactome : Influenza Viral RNA Transcription and Replication
Reactome : Insulin receptor signalling cascade
Reactome : L13a-mediated translational silencing of Ceruloplasmin expression
Reactome : Metabolism of RNA
Reactome : Metabolism of mRNA
Reactome : Metabolism of proteins
Reactome : Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)
Reactome : Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
Reactome : Nonsense-Mediated Decay (NMD)
Reactome : PI3K Cascade
Reactome : PKB-mediated events
Reactome : Peptide chain elongation
Reactome : Ribosomal scanning and start codon recognition
Reactome : S6K1 signalling
Reactome : S6K1-mediated signalling
Reactome : SRP-dependent cotranslational protein targeting to membrane
Reactome : Signal Transduction
Reactome : Signaling by Insulin receptor
Reactome : Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome : Translation
Reactome : Translation initiation complex formation
Reactome : Viral mRNA Translation
Reactome : mTOR signalling
Reactome : mTORC1-mediated signalling
WikiPathway : Cytoplasmic Ribosomal Proteins
WikiPathway : FSH signaling pathway
WikiPathway : IL-2 Signaling pathway
WikiPathway : IL-5 signaling pathway
WikiPathway : Kit receptor signaling pathway
WikiPathway : Leptin signaling pathway
WikiPathway : Prolactin Signaling Pathway
WikiPathway : TSH signaling pathway

Protein

UniProt : P62753
UniProt : A2A3R6
HPRD : 01592

Clone

Cloning Information : 30768
HIP Master Clone ID : 61144
Original Clone ID : FLH030768.01X

Organism


TAX_ID : 9606
Species Specific ID: 6194

Genome

Chromosome : 9
Map Location : 9p21
Ensembl : ENSG00000137154

Reagents

Labome : RPS6-antibody