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EIF2S2 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  EIF2S2
Gene Name:  eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH109676.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00001845

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1002nts         Open reading frame : 1 to 1002
Reference Sequence Alignment

ATGTCTGGGGACGAGATGATTTTTGATCCTACTATGAGCAAGAAGAAAAAGAAGAAGAAG
AAGCCTTTTATGTTAGATGAGGAAGGGGATACCCAAACAGAGGAAACCCAGCCTTCAGAA
ACAAAAGAAGTGGAGCCAGAGCCAACTGAGGACAAGGATTTGGAAGCTGATGAAGAGGAC
ACTAGGAAAAAAGATGCTTCTGATGATCTAGATGACTTGAACTTCTTTAATCAAAAGAAA
AAGAAGAAAAAAACTAAAAAGATATTTGATATTGATGAAGCTGAAGAAGGTGTAAAGGAT
CTTAAGATTGAAAGTGATGTTCAAGAACCAACTGAACCAGAGGATGACCTTGACATTATG
CTTGGCAATAAAAAGAAGAAAAAGAAGAATGTTAAGTTCCCAGATGAGGATGAAATACTA
GAGAAAGATGAAGCTCTAGAAGATGAAGACAACAAAAAAGATGATGGTATCTCATTCAGT
AATCAGACAGGCCCTGCTTGGGCAGGCTCAGAAAGAGACTACACATACGAGGAGCTGCTG
AATCGAGTGTTCAACATCATGAGGGAAAAGAATCCAGATATGGTTGCTGGGGAGAAAAGG
AAATTTGTCATGAAACCTCCACAAGTCGTCCGAGTAGGAACCAAGAAAACTTCTTTTGTC
AACTTTACAGATATCTGTAAACTATTACATCGTCAGCCCAAACATCTCCTTGCATTTTTG
TTGGCTGAATTGGGTACAAGTGGTTCTATAGATGGTAATAACCAACTTGTAATCAAAGGA
AGATTCCAACAGAAACAGATAGAAAATGTCTTGAGAAGATATATCAAGGAATATGTCACT
TGTCACACATGCCGATCACCGGACACAATCCTGCAGAAGGACACACGACTCTATTTCCTA
CAGTGCGAAACTTGTCATTCTAGATGTTCTGTTGCCAGTATCAAAACCGGCTTCCAGGCT
GTCACGGGCAAGCGAGCACAGCTCCGTGCCAAAGCTAACTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1147
Start on reference sequence 146
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : EIF2S2
Symbol Nomenclature : EIF2S2
SYNONYM : EIF2; EIF2B; EIF2beta; PPP1R67; eIF-2-beta
Designation : eukaryotic translation initiation factor 2 subunit 2|eukaryotic translation initiation factor 2 subunit beta|protein phosphatase 1, regulatory subunit 67
Full Nomenclature : eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa
GENEID : 8894
GI : 61359363
GenBank Accession : AY888765
HGNC : 3266
MIM : 603908
Vega : OTTHUMG00000032287
Target GenBank: BC000461

 

Reference Sequence Alignment

  

HsCD00001845        ATGTCTGGGGACGAGATGATTTTTGATCCTACTATGAGCAAGAAGAAAAAGAAGAAGAAG
NM_003908.3         ATGTCTGGGGACGAGATGATTTTTGATCCTACTATGAGCAAGAAGAAAAAGAAGAAGAAG
XM_005260605.1      ATGTCTGGGGACGAGATGATTTTTGATCCTACTATGAGCAAGAAGAAAAAGAAGAAGAAG
                    ************************************************************

HsCD00001845        AAGCCTTTTATGTTAGATGAGGAAGGGGATACCCAAACAGAGGAAACCCAGCCTTCAGAA
NM_003908.3         AAGCCTTTTATGTTAGATGAGGAAGGGGATACCCAAACAGAGGAAACCCAGCCTTCAGAA
XM_005260605.1      AAGCCTTTTATGTTAGATGAGGAAGGGGATACCCAAACAGAGGAAACCCAGCCTTCAGAA
                    ************************************************************

HsCD00001845        ACAAAAGAAGTGGAGCCAGAGCCAACTGAGGACAAGGATTTGGAAGCTGATGAAGAGGAC
NM_003908.3         ACAAAAGAAGTGGAGCCAGAGCCAACTGAGGACAAGGATTTGGAAGCTGATGAAGAGGAC
XM_005260605.1      ACAAAAGAAGTGGAGCCAGAGCCAACTGAGGACAAGGATTTGGAAGCTGATGAAGAGGAC
                    ************************************************************

HsCD00001845        ACTAGGAAAAAAGATGCTTCTGATGATCTAGATGACTTGAACTTCTTTAATCAAAAGAAA
NM_003908.3         ACTAGGAAAAAAGATGCTTCTGATGATCTAGATGACTTGAACTTCTTTAATCAAAAGAAA
XM_005260605.1      ACTAGGAAAAAAGATGCTTCTGATGATCTAGATGACTTGAACTTCTTTAATCAAAAGAAA
                    ************************************************************

HsCD00001845        AAGAAGAAAAAAACTAAAAAGATATTTGATATTGATGAAGCTGAAGAAGGTGTAAAGGAT
NM_003908.3         AAGAAGAAAAAAACTAAAAAGATATTTGATATTGATGAAGCTGAAGAAGGTGTAAAGGAT
XM_005260605.1      AAGAAGAAAAAAACTAAAAAGATATTTGATATTGATGAAGCTGAAGAAGGTGTAAAGGAT
                    ************************************************************

HsCD00001845        CTTAAGATTGAAAGTGATGTTCAAGAACCAACTGAACCAGAGGATGACCTTGACATTATG
NM_003908.3         CTTAAGATTGAAAGTGATGTTCAAGAACCAACTGAACCAGAGGATGACCTTGACATTATG
XM_005260605.1      CTTAAGATTGAAAGTGATGTTCAAGAACCAACTGAACCAGAGGATGACCTTGACATTATG
                    ************************************************************

HsCD00001845        CTTGGCAATAAAAAGAAGAAAAAGAAGAATGTTAAGTTCCCAGATGAGGATGAAATACTA
NM_003908.3         CTTGGCAATAAAAAGAAGAAAAAGAAGAATGTTAAGTTCCCAGATGAGGATGAAATACTA
XM_005260605.1      CTTGGCAATAAAAAGAAGAAAAAGAAGAATGTTAAGTTCCCAGATGAGGATGAAATACTA
                    ************************************************************

HsCD00001845        GAGAAAGATGAAGCTCTAGAAGATGAAGACAACAAAAAAGATGATGGTATCTCATTCAGT
NM_003908.3         GAGAAAGATGAAGCTCTAGAAGATGAAGACAACAAAAAAGATGATGGTATCTCATTCAGT
XM_005260605.1      GAGAAAGATGAAGCTCTAGAAGATGAAGACAACAAAAAAGATGATGGTATCTCATTCAGT
                    ************************************************************

HsCD00001845        AATCAGACAGGCCCTGCTTGGGCAGGCTCAGAAAGAGACTACACATACGAGGAGCTGCTG
NM_003908.3         AATCAGACAGGCCCTGCTTGGGCAGGCTCAGAAAGAGACTACACATACGAGGAGCTGCTG
XM_005260605.1      AATCAGACAGGCCCTGCTTGGGCAGGCTCAGAAAGAGACTACACATACGAGGAGCTGCTG
                    ************************************************************

HsCD00001845        AATCGAGTGTTCAACATCATGAGGGAAAAGAATCCAGATATGGTTGCTGGGGAGAAAAGG
NM_003908.3         AATCGAGTGTTCAACATCATGAGGGAAAAGAATCCAGATATGGTTGCTGGGGAGAAAAGG
XM_005260605.1      AATCGAGTGTTCAACATCATGAGGGAAAAGAATCCAGATATGGTTGCTGGGGAGAAAAGG
                    ************************************************************

HsCD00001845        AAATTTGTCATGAAACCTCCACAAGTCGTCCGAGTAGGAACCAAGAAAACTTCTTTTGTC
NM_003908.3         AAATTTGTCATGAAACCTCCACAAGTCGTCCGAGTAGGAACCAAGAAAACTTCTTTTGTC
XM_005260605.1      AAATTTGTCATGAAACCTCCACAAGTCGTCCGAGTAGGAACCAAGAAAACTTCTTTTGTC
                    ************************************************************

HsCD00001845        AACTTTACAGATATCTGTAAACTATTACATCGTCAGCCCAAACATCTCCTTGCATTTTTG
NM_003908.3         AACTTTACAGATATCTGTAAACTATTACATCGTCAGCCCAAACATCTCCTTGCATTTTTG
XM_005260605.1      AACTTTACAGATATCTGTAAACTTG-----------------------------------
                    ***********************:                                    

HsCD00001845        TTGGCTGAATTGGGTACAAGTGGTTCTATAGATGGTAATAACCAACTTGTAATCAAAGGA
NM_003908.3         TTGGCTGAATTGGGTACAAGTGGTTCTATAGATGGTAATAACCAACTTGTAATCAAAGGA
XM_005260605.1      ----------------------GTTCTATAGATGGTAATAACCAACTTGTAATCAAAGGA
                                          **************************************

HsCD00001845        AGATTCCAACAGAAACAGATAGAAAATGTCTTGAGAAGATATATCAAGGAATATGTCACT
NM_003908.3         AGATTCCAACAGAAACAGATAGAAAATGTCTTGAGAAGATATATCAAGGAATATGTCACT
XM_005260605.1      AGATTCCAACAGAAACAGATAGAAAATGTCTTGAGAAGATATATCAAGGAATATGTCACT
                    ************************************************************

HsCD00001845        TGTCACACATGCCGATCACCGGACACAATCCTGCAGAAGGACACACGACTCTATTTCCTA
NM_003908.3         TGTCACACATGCCGATCACCGGACACAATCCTGCAGAAGGACACACGACTCTATTTCCTA
XM_005260605.1      TGTCACACATGCCGATCACCGGACACAATCCTGCAGAAGGACACACGACTCTATTTCCTA
                    ************************************************************

HsCD00001845        CAGTGCGAAACTTGTCATTCTAGATGTTCTGTTGCCAGTATCAAAACCGGCTTCCAGGCT
NM_003908.3         CAGTGCGAAACTTGTCATTCTAGATGTTCTGTTGCCAGTATCAAAACCGGCTTCCAGGCT
XM_005260605.1      CAGTGCGAAACTTGTCATTCTAGATGTTCTGTTGCCAGTATCAAAACCGGCTTCCAGGCT
                    ************************************************************

HsCD00001845        GTCACGGGCAAGCGAGCACAGCTCCGTGCCAAAGCTAACTAG
NM_003908.3         GTCACGGGCAAGCGAGCACAGCTCCGTGCCAAAGCTAACTAA
XM_005260605.1      GTCACGGGCAAGCGAGCACAGCTCCGTGCCAAAGCTAACTAA
                    *****************************************.

Pathways

Reactome : 3' -UTR-mediated translational regulation
Reactome : Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
Reactome : Cap-dependent Translation Initiation
Reactome : Eukaryotic Translation Initiation
Reactome : Formation of the ternary complex, and subsequently, the 43S complex
Reactome : GTP hydrolysis and joining of the 60S ribosomal subunit
Reactome : Gene Expression
Reactome : L13a-mediated translational silencing of Ceruloplasmin expression
Reactome : Metabolism of proteins
Reactome : Recycling of eIF2:GDP
Reactome : Ribosomal scanning and start codon recognition
Reactome : Translation
Reactome : Translation initiation complex formation
WikiPathway : Translation Factors

Clone

Cloning Information : 109676
HIP Master Clone ID : 85959
Original Clone ID : FLH109676.01X

Organism


TAX_ID : 9606
Species Specific ID: 8894

Genome

Chromosome : 20
Map Location : 20q11.2
Ensembl : ENSG00000125977

Reagents

Labome : EIF2S2-antibody