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CSTF2 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CSTF2
Gene Name:  cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH015533.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00001919

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1734nts         Open reading frame : 1 to 1734
Reference Sequence Alignment

ATGGCGGGTTTGACTGTGAGAGACCCAGCGGTGGATCGTTCTCTACGTTCTGTGTTCGTG
GGGAACATTCCTTATGAAGCTACTGAAGAGCAGTTGAAGGACATCTTTTCTGAGGTTGGA
CCTGTTGTTAGTTTCAGATTGGTATACGATAGAGAGACAGGAAAGCCAAAGGGTTATGGC
TTCTGTGAATACCAAGACCAAGAGACAGCACTTAGTGCCATGCGGAACCTGAATGGGCGC
GAATTCAGTGGGAGAGCACTTCGAGTGGACAATGCTGCCAGTGAAAAGAACAAAGAAGAG
CTGAAGAGCCTTGGCACTGGTGCCCCTGTCATTGAGTCACCTTATGGAGAGACCATCAGT
CCTGAGGATGCCCCTGAGTCCATTAGCAAAGCAGTTGCCAGCCTTCCACCAGAGCAGATG
TTTGAGCTGATGAAACAAATGAAGCTCTGTGTCCAGAATAGTCCCCAGGAGGCACGGAAC
ATGTTACTTCAGAACCCTCAACTGGCTTATGCTTTGCTGCAAGCACAGGTAGTGATGAGA
ATTGTGGATCCGGAAATTGCCCTGAAAATTCTGCATCGCCAGACAAATATCCCAACGCTG
ATTGCAGGCAACCCTCAGCCAGTCCATGGTGCTGGGCCTGGCTCAGGATCCAATGTGTCA
ATGAACCAGCAGAATCCTCAGGCCCCTCAGGCCCAGTCTTTGGGTGGAATGCATGTCAAT
GGCGCACCTCCTCTGATGCAAGCTTCTATGCAGGGTGGAGTTCCAGCACCAGGGCAAATG
CCAGCTGCTGTCACAGGACCTGGCCCTGGTTCCTTAGCTCCTGGAGGAGGAATGCAGGCT
CAGGTTGGAATGCCAGGAAGTGGACCAGTGTCCATGGAACGGGGGCAAGTGCCGATGCAA
GACCCCAGAGCAGCTATGCAGCGGGGATCCTTGCCTGCGAATGTCCCAACCCCTCGAGGC
TTGTTAGGAGATGCTCCGAATGATCCACGGGGAGGCACTTTACTTTCTGTAACTGGAGAG
GTAGAGCCTAGAGGTTACTTGGGACCACCTCATCAGGGTCCACCCATGCACCATGTCCCT
GGCCATGAGAGCCGAGGACCACCCCCACATGAACTGAGGGGAGGGCCATTACCCGAGCCC
AGACCTCTAATGGCAGAACCAAGAGGACCCATGCTAGATCAGAGGGGTCCACCCTTGGAT
GGCAGAGGTGGAAGGGATCCCCGAGGAATAGATGCACGAGGGATGGAGGCCCGAGCCATG
GAGGCAAGAGGGTTAGATGCCAGAGGATTAGAGGCCCGTGCAATGGAGGCCCGTGCGATG
GAAGCTCGTGCAATGGAGGCCCGAGCGATGGAGGCCCGTGCAATGGAAGTCCGAGGGATG
GAGGCCAGAGGCATGGATACCAGAGGCCCAGTGCCTGGCCCCAGAGGACCTATACCTAGT
GGAATGCAGGGTCCCAGTCCAATTAACATGGGGGCGGTTGTCCCCCAGGGATCCAGACAG
GTCCCAGTCATGCAGGGAACAGGAATGCAAGGAGCAAGTATACAGGGTGGAAGCCAGCCT
GGCGGCTTTAGTCCCGGGCAGAACCAAGTCACTCCACAGGATCATGAGAAGGCTGCTTTG
ATTATGCAGGTTCTACAACTGACTGCAGACCAGATTGCCATGTTGCCTCCTGAGCAAAGG
CAGAGTATCCTGATTTTAAAGGAACAAATACAGAAATCCACTGGAGCACCTTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1761
Start on reference sequence 28
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CSTF2
Symbol Nomenclature : CSTF2
SYNONYM : CstF-64
Designation : CF-1 64 kDa subunit|CSTF 64 kDa subunit|betaCstF-64 variant 2|cleavage stimulation factor 64 kDa subunit|cleavage stimulation factor subunit 2
Full Nomenclature : cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
GENEID : 1478
Locus Tag : RP1-146H21.2
GI : 61359615
GenBank Accession : AY888801
HGNC : 2484
MIM : 300907
Vega : OTTHUMG00000022709
Target GenBank: BC017712

 

Reference Sequence Alignment

  

HsCD00001919      ATGGCGGGTTTGACTGTGAGAGACCCAGCGGTGGATCGTTCTCTACGTTCTGTGTTCGTG
NM_001325.2       ATGGCGGGTTTGACTGTGAGAGACCCAGCGGTGGATCGTTCTCTACGTTCTGTGTTCGTG
                  ************************************************************

HsCD00001919      GGGAACATTCCTTATGAAGCTACTGAAGAGCAGTTGAAGGACATCTTTTCTGAGGTTGGA
NM_001325.2       GGGAACATTCCTTATGAAGCTACTGAAGAGCAGTTGAAGGACATCTTTTCTGAGGTTGGA
                  ************************************************************

HsCD00001919      CCTGTTGTTAGTTTCAGATTGGTATACGATAGAGAGACAGGAAAGCCAAAGGGTTATGGC
NM_001325.2       CCTGTTGTTAGTTTCAGATTGGTATACGATAGAGAGACAGGAAAGCCAAAGGGTTATGGC
                  ************************************************************

HsCD00001919      TTCTGTGAATACCAAGACCAAGAGACAGCACTTAGTGCCATGCGGAACCTGAATGGGCGC
NM_001325.2       TTCTGTGAATACCAAGACCAAGAGACAGCACTTAGTGCCATGCGGAACCTGAATGGGCGC
                  ************************************************************

HsCD00001919      GAATTCAGTGGGAGAGCACTTCGAGTGGACAATGCTGCCAGTGAAAAGAACAAAGAAGAG
NM_001325.2       GAATTCAGTGGGAGAGCACTTCGAGTGGACAATGCTGCCAGTGAAAAGAACAAAGAAGAG
                  ************************************************************

HsCD00001919      CTGAAGAGCCTTGGCACTGGTGCCCCTGTCATTGAGTCACCTTATGGAGAGACCATCAGT
NM_001325.2       CTGAAGAGCCTTGGCACTGGTGCCCCTGTCATTGAGTCACCTTATGGAGAGACCATCAGT
                  ************************************************************

HsCD00001919      CCTGAGGATGCCCCTGAGTCCATTAGCAAAGCAGTTGCCAGCCTTCCACCAGAGCAGATG
NM_001325.2       CCTGAGGATGCCCCTGAGTCCATTAGCAAAGCAGTTGCCAGCCTTCCACCAGAGCAGATG
                  ************************************************************

HsCD00001919      TTTGAGCTGATGAAACAAATGAAGCTCTGTGTCCAGAATAGTCCCCAGGAGGCACGGAAC
NM_001325.2       TTTGAGCTGATGAAACAAATGAAGCTCTGTGTCCAGAATAGTCCCCAGGAGGCACGGAAC
                  ************************************************************

HsCD00001919      ATGTTACTTCAGAACCCTCAACTGGCTTATGCTTTGCTGCAAGCACAGGTAGTGATGAGA
NM_001325.2       ATGTTACTTCAGAACCCTCAACTGGCTTATGCTTTGCTGCAAGCACAGGTAGTGATGAGA
                  ************************************************************

HsCD00001919      ATTGTGGATCCGGAAATTGCCCTGAAAATTCTGCATCGCCAGACAAATATCCCAACGCTG
NM_001325.2       ATTGTGGATCCGGAAATTGCCCTGAAAATTCTGCATCGCCAGACAAATATCCCAACGCTG
                  ************************************************************

HsCD00001919      ATTGCAGGCAACCCTCAGCCAGTCCATGGTGCTGGGCCTGGCTCAGGATCCAATGTGTCA
NM_001325.2       ATTGCAGGCAACCCTCAGCCAGTCCATGGTGCTGGGCCTGGCTCAGGATCCAATGTGTCA
                  ************************************************************

HsCD00001919      ATGAACCAGCAGAATCCTCAGGCCCCTCAGGCCCAGTCTTTGGGTGGAATGCATGTCAAT
NM_001325.2       ATGAACCAGCAGAATCCTCAGGCCCCTCAGGCCCAGTCTTTGGGTGGAATGCATGTCAAT
                  ************************************************************

HsCD00001919      GGCGCACCTCCTCTGATGCAAGCTTCTATGCAGGGTGGAGTTCCAGCACCAGGGCAAATG
NM_001325.2       GGCGCACCTCCTCTGATGCAAGCTTCTATGCAGGGTGGAGTTCCAGCACCAGGGCAAATG
                  ************************************************************

HsCD00001919      CCAGCTGCTGTCACAGGACCTGGCCCTGGTTCCTTAGCTCCTGGAGGAGGAATGCAGGCT
NM_001325.2       CCAGCTGCTGTCACAGGACCTGGCCCTGGTTCCTTAGCTCCTGGAGGAGGAATGCAGGCT
                  ************************************************************

HsCD00001919      CAGGTTGGAATGCCAGGAAGTGGACCAGTGTCCATGGAACGGGGGCAAGTGCCGATGCAA
NM_001325.2       CAGGTTGGAATGCCAGGAAGTGGACCAGTGTCCATGGAACGGGGGCAAGTGCCGATGCAA
                  ************************************************************

HsCD00001919      GACCCCAGAGCAGCTATGCAGCGGGGATCCTTGCCTGCGAATGTCCCAACCCCTCGAGGC
NM_001325.2       GACCCCAGAGCAGCTATGCAGCGGGGATCCTTGCCTGCGAATGTCCCAACCCCTCGAGGC
                  ************************************************************

HsCD00001919      TTGTTAGGAGATGCTCCGAATGATCCACGGGGAGGCACTTTACTTTCTGTAACTGGAGAG
NM_001325.2       TTGTTAGGAGATGCTCCGAATGATCCACGGGGAGGCACTTTACTTTCTGTAACTGGAGAG
                  ************************************************************

HsCD00001919      GTAGAGCCTAGAGGTTACTTGGGACCACCTCATCAGGGTCCACCCATGCACCATGTCCCT
NM_001325.2       GTAGAGCCTAGAGGTTACTTGGGACCACCTCATCAGGGTCCACCCATGCACCATGTCCCT
                  ************************************************************

HsCD00001919      GGCCATGAGAGCCGAGGACCACCCCCACATGAACTGAGGGGAGGGCCATTACCCGAGCCC
NM_001325.2       GGCCATGAGAGCCGAGGACCACCCCCACATGAACTGAGGGGAGGGCCATTACCCGAGCCC
                  ************************************************************

HsCD00001919      AGACCTCTAATGGCAGAACCAAGAGGACCCATGCTAGATCAGAGGGGTCCACCCTTGGAT
NM_001325.2       AGACCTCTAATGGCAGAACCAAGAGGACCCATGCTAGATCAGAGGGGTCCACCCTTGGAT
                  ************************************************************

HsCD00001919      GGCAGAGGTGGAAGGGATCCCCGAGGAATAGATGCACGAGGGATGGAGGCCCGAGCCATG
NM_001325.2       GGCAGAGGTGGAAGGGATCCCCGAGGAATAGATGCACGAGGGATGGAGGCCCGAGCCATG
                  ************************************************************

HsCD00001919      GAGGCAAGAGGGTTAGATGCCAGAGGATTAGAGGCCCGTGCAATGGAGGCCCGTGCGATG
NM_001325.2       GAGGCAAGAGGGTTAGATGCCAGAGGATTAGAGGCCCGTGCAATGGAGGCCCGTGCGATG
                  ************************************************************

HsCD00001919      GAAGCTCGTGCAATGGAGGCCCGAGCGATGGAGGCCCGTGCAATGGAAGTCCGAGGGATG
NM_001325.2       GAAGCTCGTGCAATGGAGGCCCGAGCGATGGAGGCCCGTGCAATGGAAGTCCGAGGGATG
                  ************************************************************

HsCD00001919      GAGGCCAGAGGCATGGATACCAGAGGCCCAGTGCCTGGCCCCAGAGGACCTATACCTAGT
NM_001325.2       GAGGCCAGAGGCATGGATACCAGAGGCCCAGTGCCTGGCCCCAGAGGACCTATACCTAGT
                  ************************************************************

HsCD00001919      GGAATGCAGGGTCCCAGTCCAATTAACATGGGGGCGGTTGTCCCCCAGGGATCCAGACAG
NM_001325.2       GGAATGCAGGGTCCCAGTCCAATTAACATGGGGGCGGTTGTCCCCCAGGGATCCAGACAG
                  ************************************************************

HsCD00001919      GTCCCAGTCATGCAGGGAACAGGAATGCAAGGAGCAAGTATACAGGGTGGAAGCCAGCCT
NM_001325.2       GTCCCAGTCATGCAGGGAACAGGAATGCAAGGAGCAAGTATACAGGGTGGAAGCCAGCCT
                  ************************************************************

HsCD00001919      GGCGGCTTTAGTCCCGGGCAGAACCAAGTCACTCCACAGGATCATGAGAAGGCTGCTTTG
NM_001325.2       GGCGGCTTTAGTCCCGGGCAGAACCAAGTCACTCCACAGGATCATGAGAAGGCTGCTTTG
                  ************************************************************

HsCD00001919      ATTATGCAGGTTCTACAACTGACTGCAGACCAGATTGCCATGTTGCCTCCTGAGCAAAGG
NM_001325.2       ATTATGCAGGTTCTACAACTGACTGCAGACCAGATTGCCATGTTGCCTCCTGAGCAAAGG
                  ************************************************************

HsCD00001919      CAGAGTATCCTGATTTTAAAGGAACAAATACAGAAATCCACTGGAGCACCTTAG
NM_001325.2       CAGAGTATCCTGATTTTAAAGGAACAAATACAGAAATCCACTGGAGCACCTTGA
                  ****************************************************..

Pathways

Reactome : Cleavage of Growing Transcript in the Termination Region
Reactome : Gene Expression
Reactome : Post-Elongation Processing of Intron-Containing pre-mRNA
Reactome : Post-Elongation Processing of Intronless pre-mRNA
Reactome : Post-Elongation Processing of the Transcript
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : Processing of Capped Intronless Pre-mRNA
Reactome : Processing of Intronless Pre-mRNAs
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Termination
Reactome : Transcription
Reactome : mRNA 3'-end processing
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway
WikiPathway : mRNA processing

Protein

SMART domain : RRM : RNA recognition motif
UniProt : P33240
HPRD : 02652

Clone

Cloning Information : 15533
HIP Master Clone ID : 29732
Original Clone ID : FLH015533.01X

Organism


TAX_ID : 9606
Species Specific ID: 1478

Genome

Chromosome : X
Map Location : Xq22.1
Ensembl : ENSG00000101811

Reagents

Labome : CSTF2-antibody