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CDC23 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CDC23
Gene Name:  cell division cycle 23
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH109856.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00001952

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1776nts         Open reading frame : 1 to 1776
Reference Sequence Alignment

ATGGTCCCGGTGGCTGTGACGGCGGCAGTGGCGCCTGTCCTGTCCATAAACAGCGATTTC
TCAGATTTGCGGGAAATTAAAAAGCAACTGCTGCTTATTGCGGGCCTTACCCGGGAGCGG
GGCCTACTACACAGTAGCAAATGGTCGGCGGAGTTGGCTTTCTCTCTCCCTGCATTGCCT
CTGGCCGAGCTGCAACCGCCTCCGCCTATTACAGAGGAAGATGCCCAGGATATGGATGCC
TATACCCTGGCCAAGGCCTACTTTGACGTTAAAGAGTATGATCGGGCAGCACATTTCCTG
CATGGCTGCAATAGCAAGAAAGCCTATTTTCTGTATATGTATTCCAGATATCTGTCTGGA
GAAAAAAAGAAGGACGATGAAACAGTTGATAGCTTAGGCCCCCTGGAAAAAGGACAAGTG
AAAAATGAGGCGCTTAGAGAATTGAGAGTGGAGCTCAGCAAAAAACACCAAGCTCGAGAA
CTTGATGGATTTGGACTTTATCTGTATGGTGTGGTGCTTCGAAAACTGGACTTGGTTAAA
GAGGCCATTGATGTGTTTGTGGAAGCTACTCATGTTTTGCCCTTGCATTGGGGAGCCTGG
TTAGAACTCTGTAACCTGATCACAGACAAAGAGATGCTGAAGTTCCTGTCTTTGCCAGAC
ACCTGGATGAAAGAGTTTTTTCTGGCTCATATATACACAGAGTTGCAGTTGATAGAGGAG
GCCCTGCAAAAGTATCAGAATCTCATTGATGTGGGCTTCTCTAAGAGCTCGTATATTGTT
TCCCAAATTGCAGTTGCCTATCACAATATCAGAGATATTGACAAAGCCCTCTCCATTTTT
AATGAGCTAAGGAAACAAGACCCTTACAGGATTGAAAATATGGACACATTCTCCAACCTT
CTTTATGTCAGGAGCATGAAATCGGAGTTGAGTTATCTGGCTCATAACCTCTGTGAGATT
GATAAATATCGTGTAGAAACGTGCTGTGTAATTGGCAATTATTACAGTTTACGTTCTCAG
CATGAGAAAGCAGCCTTATATTTCCAGAGAGCCCTGAAATTAAATCCTCGGTATCTTGGT
GCCTGGACACTAATGGGACATGAGTACATGGAGATGAAGAACACGTCTGCTGCTATCCAG
GCTTATAGACATGCCATTGAGGTCAACAAACGGGACTACAGAGCTTGGTATGGCCTCGGG
CAGACCTATGAAATCCTTAAGATGCCATTTTACTGCCTTTATTATTATAGACGGGCCCAC
CAGCTTCGACCCAATGATTCTCGCATGCTGGTTGCTTTAGGAGAATGTTACGAGAAACTC
AATCAACTAGTGGAAGCCAAAAAGTGTTATTGGAGAGCTTACGCCGTGGGAGATGTGGAG
AAAATGGCTCTGGTGAAACTGGCAAAGCTTCATGAACAGTTGACTGAGTCAGAACAGGCT
GCCCAGTGTTACATCAAATATATCCAAGATATCTATTCCTGTGGGGAAATAGTAGAACAC
TTGGAGGAAAGCACTGCCTTTCGCTATCTGGCCCAGTACTATTTTAAGTGCAAACTGTGG
GATGAAGCTTCAACTTGTGCACAAAAGTGTTGTGCATTTAATGATACCCGGGAAGAAGGT
AAGGCCTTACTCCGGCAAATCCTACAGCTTCGGAACCAAGGCGAGACTCCTACCACCGAG
GTGCCTGCTCCCTTTTTCCTACCTGCTTCACTCTCTGCTAACAATACCCCCACACGCAGA
GTTTCTCCACTCAACTTGTCTTCTGTCACGCCATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1784
Start on reference sequence 9
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CDC23
Symbol Nomenclature : CDC23
SYNONYM : ANAPC8; APC8; CUT23
Designation : anaphase promoting complex subunit 8|anaphase-promoting complex subunit 8|cell division cycle 23 homolog|cell division cycle protein 23 homolog|cyclosome subunit 8
Full Nomenclature : cell division cycle 23
GENEID : 8697
GI : 61359735
GenBank Accession : AY888818
HGNC : 1724
MIM : 603462
Vega : OTTHUMG00000129198
Target GenBank: BC017713

 

Reference Sequence Alignment

  

HsCD00001952      ------------------ATGGTCCCGGTGGCTGTGACGGCGGCAGTGGCGCCTGTCCTG
NM_004661.3       ATGGCTGCGAGTACCTCCATGGTCCCGGTGGCTGTGACGGCGGCAGTGGCGCCTGTCCTG
                                    ******************************************

HsCD00001952      TCCATAAACAGCGATTTCTCAGATTTGCGGGAAATTAAAAAGCAACTGCTGCTTATTGCG
NM_004661.3       TCCATAAACAGCGATTTCTCAGATTTGCGGGAAATTAAAAAGCAACTGCTGCTTATTGCG
                  ************************************************************

HsCD00001952      GGCCTTACCCGGGAGCGGGGCCTACTACACAGTAGCAAATGGTCGGCGGAGTTGGCTTTC
NM_004661.3       GGCCTTACCCGGGAGCGGGGCCTACTACACAGTAGCAAATGGTCGGCGGAGTTGGCTTTC
                  ************************************************************

HsCD00001952      TCTCTCCCTGCATTGCCTCTGGCCGAGCTGCAACCGCCTCCGCCTATTACAGAGGAAGAT
NM_004661.3       TCTCTCCCTGCATTGCCTCTGGCCGAGCTGCAACCGCCTCCGCCTATTACAGAGGAAGAT
                  ************************************************************

HsCD00001952      GCCCAGGATATGGATGCCTATACCCTGGCCAAGGCCTACTTTGACGTTAAAGAGTATGAT
NM_004661.3       GCCCAGGATATGGATGCCTATACCCTGGCCAAGGCCTACTTTGACGTTAAAGAGTATGAT
                  ************************************************************

HsCD00001952      CGGGCAGCACATTTCCTGCATGGCTGCAATAGCAAGAAAGCCTATTTTCTGTATATGTAT
NM_004661.3       CGGGCAGCACATTTCCTGCATGGCTGCAATAGCAAGAAAGCCTATTTTCTGTATATGTAT
                  ************************************************************

HsCD00001952      TCCAGATATCTGTCTGGAGAAAAAAAGAAGGACGATGAAACAGTTGATAGCTTAGGCCCC
NM_004661.3       TCCAGATATCTGTCTGGAGAAAAAAAGAAGGACGATGAAACAGTTGATAGCTTAGGCCCC
                  ************************************************************

HsCD00001952      CTGGAAAAAGGACAAGTGAAAAATGAGGCGCTTAGAGAATTGAGAGTGGAGCTCAGCAAA
NM_004661.3       CTGGAAAAAGGACAAGTGAAAAATGAGGCGCTTAGAGAATTGAGAGTGGAGCTCAGCAAA
                  ************************************************************

HsCD00001952      AAACACCAAGCTCGAGAACTTGATGGATTTGGACTTTATCTGTATGGTGTGGTGCTTCGA
NM_004661.3       AAACACCAAGCTCGAGAACTTGATGGATTTGGACTTTATCTGTATGGTGTGGTGCTTCGA
                  ************************************************************

HsCD00001952      AAACTGGACTTGGTTAAAGAGGCCATTGATGTGTTTGTGGAAGCTACTCATGTTTTGCCC
NM_004661.3       AAACTGGACTTGGTTAAAGAGGCCATTGATGTGTTTGTGGAAGCTACTCATGTTTTGCCC
                  ************************************************************

HsCD00001952      TTGCATTGGGGAGCCTGGTTAGAACTCTGTAACCTGATCACAGACAAAGAGATGCTGAAG
NM_004661.3       TTGCATTGGGGAGCCTGGTTAGAACTCTGTAACCTGATCACAGACAAAGAGATGCTGAAG
                  ************************************************************

HsCD00001952      TTCCTGTCTTTGCCAGACACCTGGATGAAAGAGTTTTTTCTGGCTCATATATACACAGAG
NM_004661.3       TTCCTGTCTTTGCCAGACACCTGGATGAAAGAGTTTTTTCTGGCTCATATATACACAGAG
                  ************************************************************

HsCD00001952      TTGCAGTTGATAGAGGAGGCCCTGCAAAAGTATCAGAATCTCATTGATGTGGGCTTCTCT
NM_004661.3       TTGCAGTTGATAGAGGAGGCCCTGCAAAAGTATCAGAATCTCATTGATGTGGGCTTCTCT
                  ************************************************************

HsCD00001952      AAGAGCTCGTATATTGTTTCCCAAATTGCAGTTGCCTATCACAATATCAGAGATATTGAC
NM_004661.3       AAGAGCTCGTATATTGTTTCCCAAATTGCAGTTGCCTATCACAATATCAGAGATATTGAC
                  ************************************************************

HsCD00001952      AAAGCCCTCTCCATTTTTAATGAGCTAAGGAAACAAGACCCTTACAGGATTGAAAATATG
NM_004661.3       AAAGCCCTCTCCATTTTTAATGAGCTAAGGAAACAAGACCCTTACAGGATTGAAAATATG
                  ************************************************************

HsCD00001952      GACACATTCTCCAACCTTCTTTATGTCAGGAGCATGAAATCGGAGTTGAGTTATCTGGCT
NM_004661.3       GACACATTCTCCAACCTTCTTTATGTCAGGAGCATGAAATCGGAGTTGAGTTATCTGGCT
                  ************************************************************

HsCD00001952      CATAACCTCTGTGAGATTGATAAATATCGTGTAGAAACGTGCTGTGTAATTGGCAATTAT
NM_004661.3       CATAACCTCTGTGAGATTGATAAATATCGTGTAGAAACGTGCTGTGTAATTGGCAATTAT
                  ************************************************************

HsCD00001952      TACAGTTTACGTTCTCAGCATGAGAAAGCAGCCTTATATTTCCAGAGAGCCCTGAAATTA
NM_004661.3       TACAGTTTACGTTCTCAGCATGAGAAAGCAGCCTTATATTTCCAGAGAGCCCTGAAATTA
                  ************************************************************

HsCD00001952      AATCCTCGGTATCTTGGTGCCTGGACACTAATGGGACATGAGTACATGGAGATGAAGAAC
NM_004661.3       AATCCTCGGTATCTTGGTGCCTGGACACTAATGGGACATGAGTACATGGAGATGAAGAAC
                  ************************************************************

HsCD00001952      ACGTCTGCTGCTATCCAGGCTTATAGACATGCCATTGAGGTCAACAAACGGGACTACAGA
NM_004661.3       ACGTCTGCTGCTATCCAGGCTTATAGACATGCCATTGAGGTCAACAAACGGGACTACAGA
                  ************************************************************

HsCD00001952      GCTTGGTATGGCCTCGGGCAGACCTATGAAATCCTTAAGATGCCATTTTACTGCCTTTAT
NM_004661.3       GCTTGGTATGGCCTCGGGCAGACCTATGAAATCCTTAAGATGCCATTTTACTGCCTTTAT
                  ************************************************************

HsCD00001952      TATTATAGACGGGCCCACCAGCTTCGACCCAATGATTCTCGCATGCTGGTTGCTTTAGGA
NM_004661.3       TATTATAGACGGGCCCACCAGCTTCGACCCAATGATTCTCGCATGCTGGTTGCTTTAGGA
                  ************************************************************

HsCD00001952      GAATGTTACGAGAAACTCAATCAACTAGTGGAAGCCAAAAAGTGTTATTGGAGAGCTTAC
NM_004661.3       GAATGTTACGAGAAACTCAATCAACTAGTGGAAGCCAAAAAGTGTTATTGGAGAGCTTAC
                  ************************************************************

HsCD00001952      GCCGTGGGAGATGTGGAGAAAATGGCTCTGGTGAAACTGGCAAAGCTTCATGAACAGTTG
NM_004661.3       GCCGTGGGAGATGTGGAGAAAATGGCTCTGGTGAAACTGGCAAAGCTTCATGAACAGTTG
                  ************************************************************

HsCD00001952      ACTGAGTCAGAACAGGCTGCCCAGTGTTACATCAAATATATCCAAGATATCTATTCCTGT
NM_004661.3       ACTGAGTCAGAACAGGCTGCCCAGTGTTACATCAAATATATCCAAGATATCTATTCCTGT
                  ************************************************************

HsCD00001952      GGGGAAATAGTAGAACACTTGGAGGAAAGCACTGCCTTTCGCTATCTGGCCCAGTACTAT
NM_004661.3       GGGGAAATAGTAGAACACTTGGAGGAAAGCACTGCCTTTCGCTATCTGGCCCAGTACTAT
                  ************************************************************

HsCD00001952      TTTAAGTGCAAACTGTGGGATGAAGCTTCAACTTGTGCACAAAAGTGTTGTGCATTTAAT
NM_004661.3       TTTAAGTGCAAACTGTGGGATGAAGCTTCAACTTGTGCACAAAAGTGTTGTGCATTTAAT
                  ************************************************************

HsCD00001952      GATACCCGGGAAGAAGGTAAGGCCTTACTCCGGCAAATCCTACAGCTTCGGAACCAAGGC
NM_004661.3       GATACCCGGGAAGAAGGTAAGGCCTTACTCCGGCAAATCCTACAGCTTCGGAACCAAGGC
                  ************************************************************

HsCD00001952      GAGACTCCTACCACCGAGGTGCCTGCTCCCTTTTTCCTACCTGCTTCACTCTCTGCTAAC
NM_004661.3       GAGACTCCTACCACCGAGGTGCCTGCTCCCTTTTTCCTACCTGCTTCACTCTCTGCTAAC
                  ************************************************************

HsCD00001952      AATACCCCCACACGCAGAGTTTCTCCACTCAACTTGTCTTCTGTCACGCCATAG
NM_004661.3       AATACCCCCACACGCAGAGTTTCTCCACTCAACTTGTCTTCTGTCACGCCATAG
                  ******************************************************

Pathways

Reactome : APC-Cdc20 mediated degradation of Nek2A
Reactome : APC/C-mediated degradation of cell cycle proteins
Reactome : APC/C:Cdc20 mediated degradation of Cyclin B
Reactome : APC/C:Cdc20 mediated degradation of Securin
Reactome : APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome : APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
Reactome : Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Autodegradation of Cdh1 by Cdh1:APC/C
Reactome : Cdc20:Phospho-APC/C mediated degradation of Cyclin A
Reactome : Cell Cycle
Reactome : Cell Cycle Checkpoints
Reactome : Cell Cycle, Mitotic
Reactome : Cellular Senescence
Reactome : Cellular responses to stress
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
Reactome : Immune System
Reactome : Inactivation of APC/C via direct inhibition of the APC/C complex
Reactome : Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components
Reactome : M Phase
Reactome : Mitotic Anaphase
Reactome : Mitotic M-M/G1 phases
Reactome : Mitotic Metaphase and Anaphase
Reactome : Mitotic Spindle Checkpoint
Reactome : Phosphorylation of the APC/C
Reactome : Regulation of APC/C activators between G1/S and early anaphase
Reactome : Regulation of mitotic cell cycle
Reactome : Senescence-Associated Secretory Phenotype (SASP)
Reactome : Separation of Sister Chromatids

Protein

SMART domain : TPR : Tetratricopeptide repeats
UniProt : Q9UJX2
HPRD : 07221

Clone

Cloning Information : 109856
HIP Master Clone ID : 86139
Original Clone ID : FLH109856.01X

Organism


TAX_ID : 9606
Species Specific ID: 8697

Genome

Chromosome : 5
Map Location : 5q31
Ensembl : ENSG00000094880

Reagents

Labome : CDC23-antibody