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Detailed Plasmid Information

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Explanation of Terms


Gene: Gene Symbol:  None
Gene Name:  cellobiose-cellodextrin-phosphorylase, putative
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH200571.01F
Keyword: None
Species: Vibrio cholerae O1 biovar El Tor str. N16961
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
VcCD00019993

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2406nts         Open reading frame : 1 to 2406

ATGAAATACGGCTATTTCGATAATGATAATCGTGAATACGTCATCACTCGCCCTGACGTA
CCCGCACCTTGGACTAACTATCTCGGTACTGAAAAATTCTGTACGGTGATTTCACACAAC
GCAGGGGGTTACTCCTTCTATCACTCACCTGAGTACAACCGCGTGACCAAGTTTCGTCCA
AACTTTACCCAAGATCGTCCCGGGCACTATGTTTACCTGCGCGATGATGCGACAGGCGAT
TTCTGGTCTATCTCTTGGCAACCCGTTGCGAAAAGCCTTGAACAAGCGAAATACGAAGTT
CGCCACGGCTTGTCCTACTCAAAATTCAAGTGTGAATACAACGGCATTCACGCCACCAAA
ACTCTGTTTGTTCCTAAAGGCGAAGATGCCGAAGTTTGGGATGTAGTGATCGAAAATACC
TCCAACGAAGTGCGCACCATCAGTGCGTTCAACTATGTTGAGTTCTCTTTCAGCCACATC
AAGTCAGACAACCAAAACCATCAGATGTCGCTCTACTCTGCGGGAACAGCGTTCAAAGAT
GGCGTGATTGAGTATGACCTGTACTACAACACCGATGATTTCCTCGGTTTCTACTACCTG
ACTGCAACTTTCGATGCCGACAGCTACGATGGCCAACGTGACCAATTCCTTGGCATGTAC
CGCGATGAAGCCAACCCAATCGCCGTGGCGCAAGGTAAGTGCTCTAACAGTGCGCAAACC
TGTTACAACCACTGTGGTGCACTGCATAAGCAATTCGTGCTGCAACCGGGCGAGAAAGTG
CGCTTTGCGGTGATCTTAGGTGTAGGTAAAGGCAACGGCGCAAAACTGCGTGAAAAATAC
CAAGACCTGAGCAAAGTGGATTCGGCCTTTGCAGGTATCAAAGCACACTGGGATGAGCGT
TGTGCGAAATTCCAAGTGAAATCACCCAACCAAGGTCTCGATACCATGATCAACGCTTGG
ACTCTGTACCAAGCGGAAACGTGTGTGGTGTGGTCCCGTTTCGCCTCTTTCATTGAAGTC
GGCGGCCGTACAGGTCTTGGCTACCGTGATACTGCGCAAGATGCGATCTCCGTACCACAC
ACTAACCCAGCGATGACTCGTAAGCGCCTCGTTGACCTACTGCGTGGTCAAGTGAAAGCC
GGTTACGGTCTGCACCTGTTTGATCCTGACTGGTTCGATCCAGAAAAAGCGGATGTTAAA
CCGTCTAAATCACCGACAGTGGTACCAACCCCATCGGATGAAGATAAGATCCACGGCATT
AAAGATACCTGTTCTGACGATCACCTGTGGATTGTGCCAACCATCCTCAACTATGTGAAA
GAGACCGGTGACTTCGCCTTTATCGACGAAGTGATCCCTTACGCGGATGGCGGCAACGCC
ACTGTGTACGAGCACATGATGGCAGCGCTAGATTTCTCTGCAGAATATGTGGGTCAAACC
GGTATCTGTAAGGGTCTGCGTGCCGACTGGAACGACTGTTTGAACCTCGGTGGTGGTGAG
TCCTCTATGGTCTCTTTCCTACACTTCTGGGCGTTGGAATCTTTCCTTGAACTGTCACGC
TATCGCAATGATGAAGCGGCAACCGACAAGTACCAAGCGATGGCCGATGGTGTACGCGAA
GCGTGTGAAACTCACTTGTGGGATGAACAAGGCGAATGGTACATCCGTGGCCTGACCAAA
AATGGCGACAAGATCGGAACCTTCGAACAAGTGGAAGGCAAAGTGCATTTAGAGTCTAAC
TCGCTTGCAGTGTTGTCTGGCACGGTTAGCCATGAACGCGGCATCAAAGCAATGGATGCG
GTCTACAAATACCTGTTCTCCAAATACGGTCTACACCTGAACGCTCCATCATTTGCCACG
CCAAATGATGACATCGGTTTCGTGACTCGCGTTTACCAAGGCGTGAAAGAGAACGGCGCG
ATCTTCTCGCATCCAAACCCATGGGCATGGGTAGCCGAAGCGAAACTGGGCCGTGGTGAT
CGTGCGATGGAGCTGTATGACGCACTCAACCCATACAACCAAAACGACATCATCGAAACC
CGTATTGCTGAACCTTACTCTTACGTACAGTTCATCATGGGGCGTGACCACCAAGATCAC
GGCCGCGCTAACCACCCATGGTTAACCGGTACTTCTGGCTGGGCATACCATGCGACCACC
AACTATATCTTGGGTATCAAAGCGGGCTTCGATGCACTGGAGATCGATCCTTGTATCCCA
ACGTCATGGCCGGGTTTTGAAGTGACTCGCGAATGGCGTGATGCGACTTATCAGATCAAA
GTGGAAAACCCGCAAGGCGTTTCAAAAGGCGTGAAATCCATCACCCTGAATGGTCAAGCG
ATTGAAGGTGCCGTTCCTGTGCAAGCCGCAGGCAGCGTTAACCAAGTCGTGGTTGTTCTA
GGTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2406
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Reagents

Gene

Target GenBank: NP_230261

 

Clone

Cloning Information : 200571
HIP Master Clone ID : 175282
Original Clone ID : FLH200571.01F

Organism


Species Specific ID: VC0612

Reagents

Labome : VC0612-cDNA