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Explanation of Terms


Gene: Gene Symbol:  None
Gene Name:  sensory box sensor histidine kinase-response regulator
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH200640.01F
Keyword: None
Species: Vibrio cholerae O1 biovar El Tor str. N16961
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
VcCD00020054

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 3534nts         Open reading frame : 1 to 3534

ATGCGTCTATGCCCACGCTTTAGCTTGGACTTACTTGTCAAAGTAAAAAAACTCGTTAAT
TTCGTTACTTTAGGTTTTTCTTGTCTATCACCCACTCATGCTTGGCGATCGCCGCAGAGT
TTCACCGAAAGTAACACCTTAATTATGTTTAGGTTCTATCGAAAACAAAAATTCAAACGC
CTGCAAAATACCTTGATGGCTGCGTTTTTGGCCCTCAGCATTATTCCTTTGACCATTACT
GCGTTGTTTTTCCTACATTCACACAGTAAAGATCTGGAGCAGCAGAGCACTTCTTACCTC
GTTTCTGTTCGGGATAATAAACAGCAACAGGTCATCGACTACATGATGGCCAAAGAGTCG
GAAGTGATGGGTTTTGTTCGCTCCGAGCTGGCTTATGCCAGTGGCGGGCGTTTTTATGGT
CTGGTGAATGCTTTCCAGCGTTTAGACGTCAGTATTGAAGCGGCTCGTGAACATGCTCAG
CAGCGCTATATCACCGGATCGGGTGACCAAATAAAAACCTCGGTGCTCCCCCAATCAAGC
AGCTACGTGGGCAGTGAACGCTATCGACTGCTGCATAAACGCTACCACTGGGCTTACTTA
GAACTGCTGAAACGGTCGGACTTTGATGACATTTTATTGGTCGATATTGATGGCAACGTC
GTTTATTCGATTTATAAATACGACAATTTTGGCACCAACTTATTAACGGGCAAATACCAA
GACACCAATCTAGGCCATACCTTCAAGCGTTTAGAACAAACGGTCAATGAACAGCGCAAA
ACCAACGAAGATTTCACCCCTGTCGTGATTTCTGATTTCGTACAAGAGGATGGTAAACAA
TACGCTTGGCTTGGCGCACCGATTATTCAGCAAGGCTACTTGCATAGTTACGCGATGTTC
CGCTTACCAAGCAATGCGATCACCAAACTCATCGCAGAAGGCAGTAGCAATCCTTCGATG
CAAACCATTCTAGTGGGACAAGATCACCGCTCAAGAACCTTAACTTCCGCCGAAATTGCG
GTTGAAAAAAGTAAAGCTGTGGTCGATTTAGCCTTATCGGGCAAACGCGCCGTAGGCACT
TACACCAATACCGATGGTGAACAGATCATCGCGGCCTACGCACCGATCAATTTGAAAAAT
ATCCATTGGGCTCTGGTGGTTGAACTGCCAGAGAAAGAAGCGTTTGCTCGCGTTCGTCAG
TTAGAGAAACTGTTTGTTTTTGCGATGTTGACGGCTATCGTCTTAGTGGTCATCGCCTCG
CATTACCTTTCCAATTTCATTACCTCACCGCTTTTGAAGCTGACATGGGCAGCCGAACGT
GTTTCGGCAGGCGATCTTGATGAAGCCATGATCAATACCGAACGCAAAGATGAAATTGGC
CGCTTGGCGGTCAGTTTTGAGCGTATGCAGCGTTCCATTCGCGAAAAAATATCGCTGATC
AAATCGCAAAATAAAGAGTTAGAGAGCAACTTACTGATCATTCGTAAGCAAAACGATGAG
TTACAGCTAGCGAATAAGCTAAAAGATGAGTTCCTCGCGACCACATCACATGAATTACGC
ACCCCATTGCATGGCATGATTGGTATTGCCGAAGCCCTGATCTCGGGGGCAAATGGCCCG
ATTTCAGCAGCGCACAAGTATCAACTCGACATCATTATCAGCAGTGGGCAGCGCTTGGCG
ACTCTGGTTGATGATCTACTCGATTACCACAAAATGCGCTATGGCGCGCTGGATATTCAA
AGGTGTGCCGTCGATTTATCGAGCGCTACGCGCTTAGTCTTGGAGCTTTCTCACCATTTA
CTTGGCAAGAAAACGCTGCGCATTATTAATCAAGTCTCAGAACAACCGGTCTGGGTTTCT
GCGGATCCTCAACGCTTAGAGCAAGTCCTGTATAACTTGATCGGCAATGCCATTAAGTAC
ACTTCCGAAGGCAAAATTGTTATCTCCGCAACCTATATTGACGATAAAGTGCGCGTGCAG
GTGGTGGATACAGGTCAAGGGATTCCGGCAGAACAACTGGAGCACATCTTTGAGCCATTG
ATTCAAGCGGGCCATGATGCGAGTCGCTATCGCCAAGGCGCAGGACTCGGGCTTTCCATC
AGTCGTCAGTTAATCGAATTGATGCACGGCACTCTCTACGTAAGTAGTCAGCCGATGGTT
GGCACTACCTTTAGCTTCACGCTGCCTCTGGCGAGCGAAGAAGAGATTGCTCAAGCGCGC
TTGACCGAACTCCCCCACTTCCAAGCACCGGAAGTGTTGGATTCTGAACTGCCCGAGCAA
AGCAACTTACCCGAAAATGAACATGGCCCACTTCTGTTGGTCGCCGATGATGAACCAGTC
AACCTGCGTGTGCTGGACAGCTTTTTACGTCTTGAAGGTTATCGCGTACATACCGCGCAA
GATGGCCATCAAGTACTTGAAGCAATTAAACGTGAAAAACCTGAATTGGTGTTACTCGAC
ATCATGATGCCAGGCATGAGCGGTTATCAAGTGTGTGAAAAACTTCGACAAACTTACGAC
CACGCTGAACTGCCTATTATTATGCTGACGGCTCTCAACCAGTCTGATGACCGGGTACGA
GGGTTTGAAGCTGGAGCCAATGACTACTTATCCAAACCATTCAACAAACAAGAGCTGGCC
GCGCGCATTGTCGCTCACCTCACGGCGAGCAAAGCCGAACTGCGCCGCATTGAGAATGCC
CAACTGCAAAAAGAGTTGAAACATCGCGCTATGGTAGAAGCGAGCCTATTGGAAACCCAA
GGCCGATTATTGGAACAGTTAGAATCTGCGCCAGAAGCGATTTTGTGCGTTAAAGAGGGC
AATAAAGTACGTTTTGCTAACGAAGCGGCAGCACGTTTATTTAGACGTACCCCTGAGCAG
CTCAAACGCTCCAACGCCGAAGAACTGATTGCTCCTAAATTCTTGAATATTGAGCAAGAG
CATTACTGCGGCAATATTGATGTTTACGTAGATGATGTGCGCCAGCACCTCTCTGCCGAT
GTCCTTCGCTTGCCGCAAGGTTCTGGCTTACAAGCGATGTACATTTTTAATGTCGGCGGC
AGCGTCAATGCCGCGCGGATTCATAACCTCGAAACCGCGGTAGAAGCCCTGTCTAGCTAT
GCCTTCGAAGGCGATAAAGACAAGCTGCAAAAACTCAAGGAACTGGGTGGTGAGTTTACT
CGTCTTGCAGATAAAGCCTCGGGCGAGTATCAATCGAAACAAGATTTGATGCGTGCCGTG
TTAGTCGAAGCCATGACCAGTGCCTTGAACTATTGGGAACGAGTCTCAGGGCAAAGCAAG
TTCACGTTTGCCGAACAAAGTGGCTTGTGGCGCGTTTATCTTGACCGCAGCACCCTACAA
ACTCGTACCCTAGACAAATACTTACGAATTGAGACACTGCCTAAAACACCGCGTTGGCGA
ACCGTACTGAACTCGCTCGACTACATTCTTGAGCATTGCAAAGAAGCAGGCCCTGAACGC
ACTCACATCGAAATGCAGCGCGATAAATTGCAAAAACTCCTGACCAGTGAATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3534
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Reagents

Gene

Target GenBank: NP_230271

 

Clone

Cloning Information : 200640
HIP Master Clone ID : 175351
Original Clone ID : FLH200640.01F

Organism


Species Specific ID: VC0622

Reagents

Labome : VC0622-cDNA