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Human papillomavirus - 16, complete genome (Human papillomavirus type 16) in pML2d

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Explanation of Terms


Gene: Gene Symbol:  Human papillomavirus - 16, complete genome
Gene Name:  Human papillomavirus - 16, complete genome
Sequence              Map: 1203.pdf
Original Clone ID: 1203
Keyword: None
Species: Human papillomavirus type 16
Type: genome
Vector Name: pML2d               Format:  None
Source: Peter Howley
Description: None
Comments: HPV-16 sequences between NcoI and PtlM1 of p769.1 (prototype) were exchanged with the corresponding piece of CCB SiHa clone (uninterrupted E1 ORF) (p780)
Mutation/
Discrepancy :
No / Unknown
Publications: PMID: 6304740
Title: A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions
Authors: Rebecca Kramer
Peter Howley
K Munger
HpGN00021209

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: U Verification Method: 
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pML2d

pML2d in advanced viewed

Synonyms: pML, pML20, pML2D
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: cloning. (or other use?) vector; amp resistance; restriction enzyme cloning.
Comments: None
Size (bp): 2600
Parent Vector: None
Empty Vector: None
Properties: multiple cloning site
Author Name:
Publications: None
Vector Map:         Vector Sequence:
Sequence: Not Available

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (seems to be EcoRI, BamHI, SalI but not clear) 0 0
bacterial origin ori bacterial origin of replication (inferred) 0 0
selectable marker AmpR ampicillin resistance gene (inferred) 0 0

 

  • Gene
  • Clone

Gene

Target GenBank: NC_001526

 

Clone

Original Clone ID : 1203