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TP53 (Homo sapiens) in pGEM-4


Explanation of Terms

Gene: Gene Symbol:  TP53
Gene Name:  tumor protein p53 (Li-Fraumeni syndrome)
Sequence              Map: 2207.pdf
Original Clone ID: 2207-01
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pGEM-4               Format:  CLOSED
Source: Peter Howley
Description: None
Comments: None
Discrepancy :
No / Unknown
Publications: PMID: 2157286
Title: Association of human papillomavirus types 16 and 18 E6 proteins with p53
Authors: Rebecca Kramer
Peter Howley
M Scheffner

Price:  Login for Pricing
CPD only
Special MTA: None


Coding Sequence Details
Insert Sequence Verified?: U Verification Method: 
5' Linker Sequence: None
3' Linker Sequence: None


 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pGEM-4

pGEM-4 in advanced viewed

Synonyms: pGEM4
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: pGEM-series in vitro expression/cloning vector with LacZ marker, T7 and SP6; amp resistance; restriction enzyme cloning.
Comments: VectorDB notes this as old Promega vector
Size (bp): 2871
Parent Vector: None
Empty Vector: None
Properties: blue/white selection, in vitro transcription
Author Name: Promega
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, BspMI, PstI, SphI, HindIII) 0 0
bacterial origin pMB1 ori pMB1 (ColE1 and pBR322) bacterial origin 0 0
promoter SP6 SP6 promoter 0 0
promoter T7 T7 promoter 0 0
reporter gene lacZ lacZ gene 0 0
selectable marker AmpR ampicillin resistance gene 0 0


Original Clone ID: 2207-01
SYNONYM: T 1170 pGEM-p53
Species Specific ID: 7157
Target GenBank: NM_000546