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TP53 (Homo sapiens) in pGEM-4

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Explanation of Terms


Gene: Gene Symbol:  TP53
Gene Name:  tumor protein p53
Sequence              Map: 2207.pdf
Original Clone ID: 2207-01
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pGEM-4               Format:  CLOSED
Source: Peter Howley
Description: None
Comments: None
Mutation/
Discrepancy :
No / Unknown
Publications: PMID: 2157286
Title: Association of human papillomavirus types 16 and 18 E6 proteins with p53
Authors: Rebecca Kramer
Peter Howley
M Scheffner
HsCD00021216

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: U Verification Method: 
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pGEM-4

pGEM-4 in advanced viewed

Synonyms: pGEM4
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: pGEM-series in vitro expression/cloning vector with LacZ marker, T7 and SP6; amp resistance; restriction enzyme cloning.
Comments: VectorDB notes this as old Promega vector
Size (bp): 2871
Parent Vector: None
Empty Vector: None
Properties: blue/white selection, in vitro transcription
Author Name: Promega
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, BspMI, PstI, SphI, HindIII) 0 0
bacterial origin pMB1 ori pMB1 (ColE1 and pBR322) bacterial origin 0 0
promoter SP6 SP6 promoter 0 0
promoter T7 T7 promoter 0 0
reporter gene lacZ lacZ gene 0 0
selectable marker AmpR ampicillin resistance gene 0 0

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome

Gene

 

Gene Symbol : TP53
Symbol Nomenclature : TP53
SYNONYM : BCC7; LFS1; P53; TRP53
Designation : antigen NY-CO-13|cellular tumor antigen p53|mutant tumor protein 53|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53|tumor protein 53
Full Nomenclature : tumor protein p53
GENEID : 7157
SYNONYM : pGEM-p53
SYNONYM : T 1170 pGEM-p53
HGNC : 11998
MIM : 191170
Vega : OTTHUMG00000162125
Target GenBank: NM_000546

 

Pathways

NCI : AP-1 transcription factor network
NCI : Aurora A signaling
NCI : BARD1 signaling events
NCI : Direct p53 effectors
NCI : Glucocorticoid receptor regulatory network
NCI : Hypoxic and oxygen homeostasis regulation of HIF-1-alpha
NCI : LKB1 signaling events
NCI : PLK3 signaling events
NCI : Signaling events mediated by HDAC Class III
NCI : Signaling mediated by p38-alpha and p38-beta
NCI : Validated targets of C-MYC transcriptional activation
NCI : p53 pathway
NCI : p75(NTR)-mediated signaling
Panther : Apoptosis signaling pathway
Panther : Huntington disease
Panther : P53 pathway feedback loops 1
Panther : Wnt signaling pathway
Panther : p53 pathway
Panther : p53 pathway by glucose deprivation
Panther : p53 pathway feedback loops 2
Reactome : Activation of BH3-only proteins
Reactome : Activation of NOXA and translocation to mitochondria
Reactome : Activation of PUMA and translocation to mitochondria
Reactome : Apoptosis
Reactome : Autodegradation of the E3 ubiquitin ligase COP1
Reactome : Cell Cycle
Reactome : Cell Cycle Checkpoints
Reactome : Cellular Senescence
Reactome : Cellular responses to stress
Reactome : DNA Damage/Telomere Stress Induced Senescence
Reactome : Factors involved in megakaryocyte development and platelet production
Reactome : Formation of Senescence-Associated Heterochromatin Foci (SAHF)
Reactome : G1/S DNA Damage Checkpoints
Reactome : Hemostasis
Reactome : Intrinsic Pathway for Apoptosis
Reactome : Oncogene Induced Senescence
Reactome : Oxidative Stress Induced Senescence
Reactome : Pre-NOTCH Expression and Processing
Reactome : Pre-NOTCH Transcription and Translation
Reactome : Signal Transduction
Reactome : Signaling by NOTCH
Reactome : Stabilization of p53
Reactome : Transcriptional activation of cell cycle inhibitor p21
Reactome : Transcriptional activation of p53 responsive genes
Reactome : p53-Dependent G1 DNA Damage Response
Reactome : p53-Dependent G1/S DNA damage checkpoint
WikiPathway : AMPK signaling
WikiPathway : Alzheimers Disease
WikiPathway : Apoptosis
WikiPathway : Cell cycle
WikiPathway : DNA damage response
WikiPathway : DNA damage response (only ATM dependent)
WikiPathway : ErbB signaling pathway
WikiPathway : Fluoropyrimidine Activity
WikiPathway : Folate Metabolism
WikiPathway : G1 to S cell cycle control
WikiPathway : Integrated Breast Cancer Pathway
WikiPathway : Integrated Cancer pathway
WikiPathway : MAPK signaling pathway
WikiPathway : Senescence and Autophagy
WikiPathway : TGF beta Signaling Pathway
WikiPathway : TP53 network
WikiPathway : Wnt Signaling Pathway and Pluripotency
WikiPathway : miRNA regulation of DNA Damage Response
WikiPathway : miRNAs involved in DDR

Protein

HPRD : 01859

Clone

Original Clone ID : 2207-01

Organism


TAX_ID : 9606
Species Specific ID: 7157

Genome

Chromosome : 17
Map Location : 17p13.1
Ensembl : ENSG00000141510