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RAD23A (Homo sapiens) in pGEX-4T-2

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Explanation of Terms


Gene: Gene Symbol:  RAD23A
Gene Name:  RAD23 homolog A (S. cerevisiae)
Sequence              Map: 3998.pdf
Original Clone ID: 3998-01
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pGEX-4T-2               Format:  CLOSED
Source: Peter Howley
Description: None
Comments: cloned in-frame with GST
Mutation/
Discrepancy :
Yes / Unknown
Publications: PMID: 10373495
Title: Identification of HHR23A as a substrate for E6-associated protein-mediated ubiquitination
Authors: Rebecca Kramer
Peter Howley
S Kumar
HsCD00021234

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
A391G Thr to Ala

G448A Glu to Lys

G765T Silent

deleted to avoid internal initiation

Discrepancy:
None
Others:
Tag GST (from vector) In frame
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pGEX-4T-2

pGEX-4T-2 in advanced viewed

Synonyms: pGEX-4T2, pGEX4T-2, pGEX4T2
Description: pGEX-series GST fusion vector with Ptac promoter, lacI repressor, thrombin cleaveage site ORF; amp resistance; restriction enzyme cloning.
Comments: None
Size (bp): 3700
Parent Vector: None
Empty Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Amersham Pharmacia
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site 0 0
bacterial origin ori pBR322-type ColE1 origin of replication 0 0
promoter Ptac Ptac promoter 0 0
protease cleavage site Thrombin thrombin cleaveage site leu val pro arg CUT gly ser 0 0
repressor protein gene lac Iq lac I[q] lac operon repressor protein ORF 0 0
selectable marker AmpR ampicillin resistance gene 0 0
tag GST glutathione S-transferase ORF 0 0

 


Original Clone ID: 3998-01
SYNONYM: pGEX-4T-2-HHR23A
Species Specific ID: 5886
Target GenBank: NM_005053