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E6 (Human papillomavirus type 18) in pGEM-1

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Explanation of Terms


Gene: Gene Symbol:  E6
Gene Name:  HpV18gp1
Sequence              Map: 4020.pdf
Original Clone ID: 4020-01
Keyword: None
Species: Human papillomavirus type 18
Type: cDNA
Vector Name: pGEM-1               Format:  CLOSED
Source: Peter Howley
Description: None
Comments: clone donor notes that the clone does not translate well in Promega TNT coupled reticulate lysate system
Mutation/
Discrepancy :
No / Unknown
Publications: PMID: 2157286
Title: Association of human papillomavirus types 16 and 18 E6 proteins with p53
Authors: Rebecca Kramer
Peter Howley
HpCD00021235

Price:  Login for Pricing
AVAILABLE
CPD only
Special MTA: None

 


Coding Sequence Details
Insert Sequence Verified?: U Verification Method: 
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : EB5-alpha T1, T5 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pGEM-1

pGEM-1 in advanced viewed

Synonyms: pGEM1
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: pGEM-series in vitro expression/cloning vector with T7, SP6; amp resistance; restriction enzyme cloning.
Comments: More information at the Promega website
Size (bp): 2865
Parent Vector: None
Empty Vector: None
Properties: in vitro transcription, multiple cloning site
Author Name: Promega
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (HindII to EcoRI) 7 57
bacterial origin ColE E. coli origin 317 999
promoter SP6 SP6 transcription initiation site 1 7
promoter T7 T7 transcription initiation site 64 83
selectable marker AmpR ampicillin resistance gene 1097 1756

 


Original Clone ID: 4020-01
SYNONYM: pGEM 18-E6
SYNONYM: pGEM-E6 ORF wt (HPV-18)
Species Specific ID: 1489088
Target GenBank: NC_001357