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EPHA6 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  EPHA6
Gene Name:  EPH receptor A6
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH163637.01F
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors: HIP
HsCD00021315

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1644nts         Open reading frame : 1 to 1644
Reference Sequence Alignment

ATGCAATTCCCCTCGCCTCCAGCCGCGAGGAGCTCCCCGGCGCCGCAGGCAGCGTCCTCC
TCCGAAGCAGCTGCACCTGCAACTGGGCAGCCTGGACCCTCGTGCCCTGTTCCCGGGACC
TCGCGCAGGGGGCGCCCCGGGACACCCCCTGCGGGCCGGGTGGAGGAGGAAGAGGAGGAG
GAGGAAGAAGACGTGGACAAGGACCCCCATCCTACCCAGAACACCTGCCTGCGCTGCCGC
CACTTCTCTTTAAGGGAGAGGAAAAGAGAGCCTAGGAGAACCATGGGGGGCTGCGAAGTC
CGGGAATTTCTTTTGCAATTTGGTTTCTTCTTGCCTCTGCTGACAGCGTGGCCAGGCGAC
TGCAGTCACGTCTCCAACAACCAAGTTGTGTTGCTTGATACAACAACTGTACTGGGAGAG
CTAGGATGGAAAACATATCCATTAAATGGGTGGGATGCCATCACTGAAATGGATGAACAT
AATAGGCCCATTCACACATACCAGGTATGTAATGTAATGGAACCAAACCAAAACAACTGG
CTTCGTACAAACTGGATCTCCCGTGATGCAGCTCAGAAAATTTATGTGGAAATGAAATTC
ACACTAAGGGATTGTAACAGCATCCCATGGGTCTTGGGGACTTGCAAAGAAACATTTAAT
CTGTTTTATATGGAATCAGATGAGTCCCACGGAATTAAATTCAAGCCAAACCAGTATACA
AAGATCGACACAATTGCTGCTGATGAGAGTTTTACCCAGATGGATTTGGGTGATCGCATC
CTCAAACTCAACACTGAAATTCGTGAGGTGGGGCCTATAGAAAGGAAAGGATTTTATCTG
GCTTTTCAAGACATTGGGGCGTGCATTGCCCTGGTTTCAGTCCGTGTTTTCTACAAGAAA
TGCCCCTTCACTGTTCGTAACTTGGCCATGTTTCCTGATACCATTCCAAGGGTTGATTCC
TCCTCTTTGGTTGAAGTACGGGGTTCTTGTGTGAAGAGTGCTGAAGAGCGTGACACTCCT
AAACTGTATTGTGGAGCTGATGGAGATTGGCTGGTTCCTCTTGGAAGGTGCATCTGCAGT
ACAGGATATGAAGAAATTGAGGGTTCTTGCCATGCTTGCAGACCAGGATTCTATAAAGCT
TTTGCTGGGAACACAAAATGTTCTAAATGTCCTCCACACAGTTTAACATACATGGAAGCA
ACTTCTGTCTGTCAGTGTGAAAAGGGTTATTTCCGAGCTGAAAAAGACCCACCTTCTATG
GCATGTACCAGGCCACCTTCAGCTCCTAGGAATGTGGTTTTTAACATCAATGAAACAGCC
CTTATTTTGGAATGGAGCCCACCAAGTGACACAGGAGGGAGAAAAGATCTCACATACAGT
GTAATCTGTAAGAAATGTGGCTTAGACACCAGCCAGTGTGAGGACTGTGGTGGAGGACTC
CGCTTCATCCCAAGACATACAGGCCTGATCAACAATTCCGTGATAGTACTTGACTTTGTG
TCTCACGTGAATTACACCTTTGAAATAGAAGCAATGAATGGAGTTTCTGAGTTGAGTTTT
TCTCCCAAGCCATTCACAGCTATTACAGTGACCACGGATCAAGATGGTAAGTTCCACTGC
TGTTCTCTCAAAACAGACCCATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1682
Start on reference sequence 39
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : EPHA6
Symbol Nomenclature : EPHA6
SYNONYM : EHK-2; EHK2; EK12; EPA6; HEK12; PRO57066
Designation : EPH homology kinase 2|EPH-like kinase 12|ephrin receptor EphA6|ephrin type-A receptor 6
Full Nomenclature : EPH receptor A6
GENEID : 285220
Locus Tag : UNQ6114
HGNC : 19296
MIM : 600066
Vega : OTTHUMG00000159208
Target GenBank: XM_114973

 

Reference Sequence Alignment

  

HsCD00021315        ATGCAATTCCCCTCGCCTCCAGCCGCGAGGAGCTCCCCGGCGCCGCAGGCAGCGTCCTCC
NM_001080448.2      ATGCAATTCCCCTCGCCTCCAGCCGCGAGGAGCTCCCCGGCGCCGCAGGCAGCGTCCTCC
                    ************************************************************

HsCD00021315        TCCGAAGCAGCTGCACCTGCAACTGGGCAGCCTGGACCCTCGTGCCCTGTTCCCGGGACC
NM_001080448.2      TCCGAAGCAGCTGCACCTGCAACTGGGCAGCCTGGACCCTCGTGCCCTGTTCCCGGGACC
                    ************************************************************

HsCD00021315        TCGCGCAGGGGGCGCCCCGGGACACCCCCTGCGGGCCGGGTGGAGGAGGAAGAGGAGGAG
NM_001080448.2      TCGCGCAGGGGGCGCCCCGGGACACCCCCTGCGGGCCGGGTGGAGGAGGAAGAGGAGGAG
                    ************************************************************

HsCD00021315        GAGGAAGAAGACGTGGACAAGGACCCCCATCCTACCCAGAACACCTGCCTGCGCTGCCGC
NM_001080448.2      GAGGAAGAAGACGTGGACAAGGACCCCCATCCTACCCAGAACACCTGCCTGCGCTGCCGC
                    ************************************************************

HsCD00021315        CACTTCTCTTTAAGGGAGAGGAAAAGAGAGCCTAGGAGAACCATGGGGGGCTGCGAAGTC
NM_001080448.2      CACTTCTCTTTAAGGGAGAGGAAAAGAGAGCCTAGGAGAACCATGGGGGGCTGCGAAGTC
                    ************************************************************

HsCD00021315        CGGGAATTTCTTTTGCAATTTGGTTTCTTCTTGCCTCTGCTGACAGCGTGGCCAGGCGAC
NM_001080448.2      CGGGAATTTCTTTTGCAATTTGGTTTCTTCTTGCCTCTGCTGACAGCGTGGCCAGGCGAC
                    ************************************************************

HsCD00021315        TGCAGTCACGTCTCCAACAACCAAGTTGTGTTGCTTGATACAACAACTGTACTGGGAGAG
NM_001080448.2      TGCAGTCACGTCTCCAACAACCAAGTTGTGTTGCTTGATACAACAACTGTACTGGGAGAG
                    ************************************************************

HsCD00021315        CTAGGATGGAAAACATATCCATTAAATGGGTGGGATGCCATCACTGAAATGGATGAACAT
NM_001080448.2      CTAGGATGGAAAACATATCCATTAAATGGGTGGGATGCCATCACTGAAATGGATGAACAT
                    ************************************************************

HsCD00021315        AATAGGCCCATTCACACATACCAGGTATGTAATGTAATGGAACCAAACCAAAACAACTGG
NM_001080448.2      AATAGGCCCATTCACACATACCAGGTATGTAATGTAATGGAACCAAACCAAAACAACTGG
                    ************************************************************

HsCD00021315        CTTCGTACAAACTGGATCTCCCGTGATGCAGCTCAGAAAATTTATGTGGAAATGAAATTC
NM_001080448.2      CTTCGTACAAACTGGATCTCCCGTGATGCAGCTCAGAAAATTTATGTGGAAATGAAATTC
                    ************************************************************

HsCD00021315        ACACTAAGGGATTGTAACAGCATCCCATGGGTCTTGGGGACTTGCAAAGAAACATTTAAT
NM_001080448.2      ACACTAAGGGATTGTAACAGCATCCCATGGGTCTTGGGGACTTGCAAAGAAACATTTAAT
                    ************************************************************

HsCD00021315        CTGTTTTATATGGAATCAGATGAGTCCCACGGAATTAAATTCAAGCCAAACCAGTATACA
NM_001080448.2      CTGTTTTATATGGAATCAGATGAGTCCCACGGAATTAAATTCAAGCCAAACCAGTATACA
                    ************************************************************

HsCD00021315        AAGATCGACACAATTGCTGCTGATGAGAGTTTTACCCAGATGGATTTGGGTGATCGCATC
NM_001080448.2      AAGATCGACACAATTGCTGCTGATGAGAGTTTTACCCAGATGGATTTGGGTGATCGCATC
                    ************************************************************

HsCD00021315        CTCAAACTCAACACTGAAATTCGTGAGGTGGGGCCTATAGAAAGGAAAGGATTTTATCTG
NM_001080448.2      CTCAAACTCAACACTGAAATTCGTGAGGTGGGGCCTATAGAAAGGAAAGGATTTTATCTG
                    ************************************************************

HsCD00021315        GCTTTTCAAGACATTGGGGCGTGCATTGCCCTGGTTTCAGTCCGTGTTTTCTACAAGAAA
NM_001080448.2      GCTTTTCAAGACATTGGGGCGTGCATTGCCCTGGTTTCAGTCCGTGTTTTCTACAAGAAA
                    ************************************************************

HsCD00021315        TGCCCCTTCACTGTTCGTAACTTGGCCATGTTTCCTGATACCATTCCAAGGGTTGATTCC
NM_001080448.2      TGCCCCTTCACTGTTCGTAACTTGGCCATGTTTCCTGATACCATTCCAAGGGTTGATTCC
                    ************************************************************

HsCD00021315        TCCTCTTTGGTTGAAGTACGGGGTTCTTGTGTGAAGAGTGCTGAAGAGCGTGACACTCCT
NM_001080448.2      TCCTCTTTGGTTGAAGTACGGGGTTCTTGTGTGAAGAGTGCTGAAGAGCGTGACACTCCT
                    ************************************************************

HsCD00021315        AAACTGTATTGTGGAGCTGATGGAGATTGGCTGGTTCCTCTTGGAAGGTGCATCTGCAGT
NM_001080448.2      AAACTGTATTGTGGAGCTGATGGAGATTGGCTGGTTCCTCTTGGAAGGTGCATCTGCAGT
                    ************************************************************

HsCD00021315        ACAGGATATGAAGAAATTGAGGGTTCTTGCCATGCTTGCAGACCAGGATTCTATAAAGCT
NM_001080448.2      ACAGGATATGAAGAAATTGAGGGTTCTTGCCATGCTTGCAGACCAGGATTCTATAAAGCT
                    ************************************************************

HsCD00021315        TTTGCTGGGAACACAAAATGTTCTAAATGTCCTCCACACAGTTTAACATACATGGAAGCA
NM_001080448.2      TTTGCTGGGAACACAAAATGTTCTAAATGTCCTCCACACAGTTTAACATACATGGAAGCA
                    ************************************************************

HsCD00021315        ACTTCTGTCTGTCAGTGTGAAAAGGGTTATTTCCGAGCTGAAAAAGACCCACCTTCTATG
NM_001080448.2      ACTTCTGTCTGTCAGTGTGAAAAGGGTTATTTCCGAGCTGAAAAAGACCCACCTTCTATG
                    ************************************************************

HsCD00021315        GCATGTACCAGGCCACCTTCAGCTCCTAGGAATGTGGTTTTTAACATCAATGAAACAGCC
NM_001080448.2      GCATGTACCAGGCCACCTTCAGCTCCTAGGAATGTGGTTTTTAACATCAATGAAACAGCC
                    ************************************************************

HsCD00021315        CTTATTTTGGAATGGAGCCCACCAAGTGACACAGGAGGGAGAAAAGATCTCACATACAGT
NM_001080448.2      CTTATTTTGGAATGGAGCCCACCAAGTGACACAGGAGGGAGAAAAGATCTCACATACAGT
                    ************************************************************

HsCD00021315        GTAATCTGTAAGAAATGTGGCTTAGACACCAGCCAGTGTGAGGACTGTGGTGGAGGACTC
NM_001080448.2      GTAATCTGTAAGAAATGTGGCTTAGACACCAGCCAGTGTGAGGACTGTGGTGGAGGACTC
                    ************************************************************

HsCD00021315        CGCTTCATCCCAAGACATACAGGCCTGATCAACAATTCCGTGATAGTACTTGACTTTGTG
NM_001080448.2      CGCTTCATCCCAAGACATACAGGCCTGATCAACAATTCCGTGATAGTACTTGACTTTGTG
                    ************************************************************

HsCD00021315        TCTCACGTGAATTACACCTTTGAAATAGAAGCAATGAATGGAGTTTCTGAGTTGAGTTTT
NM_001080448.2      TCTCACGTGAATTACACCTTTGAAATAGAAGCAATGAATGGAGTTTCTGAGTTGAGTTTT
                    ************************************************************

HsCD00021315        TCTCCCAAGCCATTCACAGCTATTACAGTGACCACGGATCAAGATGGTAAGTTCCACTGC
NM_001080448.2      TCTCCCAAGCCATTCACAGCTATTACAGTGACCACGGATCAAGATGCACCTTCCCTGATA
                    ********************************************** :.. * **: : .

HsCD00021315        TGTTCTCTCAAAACAGACCCATTG------------------------------------
NM_001080448.2      GGTGTGGTAAGGAAGGACTGGGCATCCCAAAATAGCATTGCCCTATCATGGCAAGCACCT
                     **    *.*..*..***  .  .                                    

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      GCTTTTTCCAATGGAGCCATTCTGGACTACGAGATCAAGTACTATGAGAAAGAACATGAG
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      CAGCTGACCTACTCTTCCACAAGGTCCAAAGCCCCCAGTGTCATCATCACAGGTCTTAAG
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      CCAGCCACCAAATATGTATTTCACATCCGAGTGAGAACTGCGACAGGATACAGTGGCTAC
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      AGTCAGAAATTTGAATTTGAAACAGGAGATGAAACTTCTGACATGGCAGCAGAACAAGGA
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      CAGATTCTCGTGATAGCCACCGCCGCTGTTGGCGGATTCACTCTCCTCGTCATCCTCACT
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      TTATTCTTCTTGATCACTGGGAGATGTCAGTGGTACATAAAAGCCAAGATGAAGTCAGAA
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      GAGAAGAGAAGAAACCACTTACAGAATGGGCATTTGCGCTTCCCGGGAATTAAAACTTAC
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      ATTGATCCAGATACATATGAAGACCCATCCCTAGCAGTCCATGAATTTGCAAAGGAGATT
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      GATCCCTCAAGAATTCGTATTGAGAGAGTCATTGGGGCAGGTGAATTTGGAGAAGTCTGT
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      AGTGGGCGTTTGAAGACACCAGGGAAAAGAGAGATCCCAGTTGCCATTAAAACTTTGAAA
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      GGTGGCCACATGGATCGGCAAAGAAGAGATTTTCTAAGAGAAGCTAGTATCATGGGCCAG
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      TTTGACCATCCAAACATCATTCGCCTAGAAGGGGTTGTCACCAAAAGATCCTTCCCGGCC
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      ATTGGGGTGGAGGCGTTTTGCCCCAGCTTCCTGAGGGCAGGGTTTTTAAATAGCATCCAG
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      GCCCCGCATCCAGTGCCAGGGGGAGGATCTTTGCCCCCCAGGATTCCTGCTGGCAGACCA
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      GTAATGATTGTGGTGGAATATATGGAGAATGGATCCCTAGACTCCTTTTTGCGGAAGCAT
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      GATGGCCACTTCACAGTCATCCAGTTGGTCGGAATGCTCCGAGGCATTGCATCAGGCATG
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      AAGTATCTTTCTGATATGGGTTATGTTCATCGAGACCTAGCGGCTCGGAATATACTGGTC
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      AATAGCAACTTAGTATGCAAAGTTTCTGATTTTGGTCTCTCCAGAGTGCTGGAAGATGAT
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      CCAGAAGCTGCTTATACAACAACTGGTGGAAAAATCCCCATAAGGTGGACAGCCCCAGAA
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      GCCATCGCCTACAGAAAATTCTCCTCAGCAAGCGATGCATGGAGCTATGGCATTGTCATG
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      TGGGAGGTCATGTCCTATGGAGAGAGACCTTATTGGGAAATGTCTAACCAAGATGTCATT
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      CTGTCCATTGAAGAAGGGTACAGACTTCCAGCTCCCATGGGCTGTCCAGCATCTCTACAC
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      CAGCTGATGCTCCACTGCTGGCAGAAGGAGAGAAATCACAGACCAAAATTTACTGACATT
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      GTCAGCTTCCTTGACAAACTGATCCGAAATCCCAGTGCCCTTCACACCCTGGTGGAGGAC
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      ATCCTTGTAATGCCAGAGTCCCCTGGTGAAGTTCCGGAATATCCTTTGTTTGTCACAGTT
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      GGTGACTGGCTAGATTCTATAAAGATGGGGCAATACAAGAATAACTTCGTGGCAGCAGGG
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      TTTACAACATTTGACCTGATTTCAAGAATGAGCATTGATGACATTAGAAGAATTGGAGTC
                                                                                

HsCD00021315        ------------------------------------------------------------
NM_001080448.2      ATACTTATTGGACACCAGAGACGAATAGTCAGCAGCATACAGACTTTACGTTTACACATG
                                                                                

HsCD00021315        ---------------------------------
NM_001080448.2      ATGCACATACAGGAGAAGGGATTTCATGTATGA
                                                     

Pathways

NCI : EPHA forward signaling
NCI : EphrinA-EPHA pathway

Protein

SMART domain : FN3 : Fibronectin type 3 domain
SMART domain : EPH_lbd : Ephrin receptor ligand binding domain
UniProt : Q9UF33
UniProt : B3KS12

Clone

Cloning Information : 163637
HIP Master Clone ID : 136837
Original Clone ID : FLH163637.01F

Organism


TAX_ID : 9606
Species Specific ID: 285220

Genome

Chromosome : 3
Map Location : 3q11.2
Ensembl : ENSG00000080224

Reagents

Labome : EPHA6-antibody