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TNFRSF10A (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TNFRSF10A
Gene Name:  tumor necrosis factor receptor superfamily, member 10a
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH013799.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00002134

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1407nts         Open reading frame : 1 to 1407
Reference Sequence Alignment

ATGGCGCCACCACCAGCTAGAGTACATCTAGGTGCGTTCCTGGCAGTGACTCCGAATCCC
GGGAGCGCAGCGAGTGGGACAGAGGCAGCCGCGGCCACACCCAGCAAAGTGTGGGGCTCT
TCCGCGGGGAGGATTGAACCACGAGGCGGGGGCCGAGGAGCGCTCCCTACCTCCATGGGA
CAGCACGGACCCAGTGCCCGGGCCCGGGCAGGGCGCGCCCCAGGACCCAGGCCGGCGCGG
GAAGCCAGCCCTCGGCTCCGGGTCCACAAGACCTTCAAGTTTGTCGTCGTCGGGGTCCTG
CTGCAGGTCGTACCTAGCTCAGCTGCAACCATCAAACTTCATGATCAATCAATTGGCACA
CAGCAATGGGAACATAGCCCTTTGGGAGAGTTGTGTCCACCAGGATCTCATAGATCAGAA
CATCCTGGAGCCTGTAACCGGTGCACAGAGGGTGTGGGTTACACCAATGCTTCCAACAAT
TTGTTTGCTTGCCTCCCATGTACAGCTTGTAAATCAGATGAAGAAGAGAGAAGTCCCTGC
ACCACGACCAGGAACACAGCATGTCAGTGCAAACCAGGAACTTTCCGGAATGACAATTCT
GCTGAGATGTGCCGGAAGTGCAGCAGAGGGTGCCCCAGAGGGATGGTCAAGGTCAAGGAT
TGTACGCCCTGGAGTGACATCGAGTGTGTCCACAAAGAATCAGGCAATGGACATAATATA
TGGGTGATTTTGGTTGTGACTTTGGTTGTTCCGTTGCTGTTGGTGGCTGTGCTGATTGTC
TGTTGTTGCATCGGCTCAGGTTGTGGAGGGGACCCCAAGTGCATGGACAGGGTGTGTTTC
TGGCGCTTGGGTCTCCTACGAGGGCCTGGGGCTGAGGACAATGCTCACAACGAGATTCTG
AGCAACGCAGACTCGCTGTCCACTTTCGTCTCTGAGCAGCAAATGGAAAGCCAGGAGCCG
GCAGATTTGACAGGTGTCACTGTACAGTCCCCAGGGGAGGCACAGTGTCTGCTGGGACCG
GCAGAAGCTGAAGGGTCTCAGAGGAGGAGGCTGCTGGTTCCAGCAAATGGTGCTGACCCC
ACTGAGACTCTGATGCTGTTCTTTGACAAGTTTGCAAACATCGTGCCCTTTGACTCCTGG
GACCAGCTCATGAGGCAGCTGGACCTCACGAAAAATGAGATCGATGTGGTCAGAGCTGGT
ACAGCAGGCCCAGGGGATGCCTTGTATGCAATGCTGATGAAATGGGTCAACAAAACTGGA
CGGAACGCCTCGATCCACACCCTGCTGGATGCCTTGGAGAGGATGGAAGAGAGACATGCA
AAAGAGAAGATTCAGGACCTCTTGGTGGACTCTGGAAAGTTCATCTACTTAGAAGATGGC
ACAGGCTCTGCCGTGTCCTTGGAGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1472
Start on reference sequence 66
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TNFRSF10A
Symbol Nomenclature : TNFRSF10A
SYNONYM : APO2; CD261; DR4; TRAILR-1; TRAILR1
Designation : TNF-related apoptosis-inducing ligand receptor 1|TRAIL receptor 1|TRAIL-R1|cytotoxic TRAIL receptor|death receptor 4|tumor necrosis factor receptor superfamily member 10A|tumor necrosis factor receptor superfamily member 10a variant 2
Full Nomenclature : tumor necrosis factor receptor superfamily, member 10a
GENEID : 8797
GI : 61370260
GenBank Accession : AY891537
HGNC : 11904
MIM : 603611
Target GenBank: BC012866

 

Reference Sequence Alignment

  

HsCD00002134      ATGGCGCCACCACCAGCTAGAGTACATCTAGGTGCGTTCCTGGCAGTGACTCCGAATCCC
NM_003844.3       ATGGCGCCACCACCAGCTAGAGTACATCTAGGTGCGTTCCTGGCAGTGACTCCGAATCCC
                  ************************************************************

HsCD00002134      GGGAGCGCAGCGAGTGGGACAGAGGCAGCCGCGGCCACACCCAGCAAAGTGTGGGGCTCT
NM_003844.3       GGGAGCGCAGCGAGTGGGACAGAGGCAGCCGCGGCCACACCCAGCAAAGTGTGGGGCTCT
                  ************************************************************

HsCD00002134      TCCGCGGGGAGGATTGAACCACGAGGCGGGGGCCGAGGAGCGCTCCCTACCTCCATGGGA
NM_003844.3       TCCGCGGGGAGGATTGAACCACGAGGCGGGGGCCGAGGAGCGCTCCCTACCTCCATGGGA
                  ************************************************************

HsCD00002134      CAGCACGGACCCAGTGCCCGGGCCCGGGCAGGGCGCGCCCCAGGACCCAGGCCGGCGCGG
NM_003844.3       CAGCACGGACCCAGTGCCCGGGCCCGGGCAGGGCGCGCCCCAGGACCCAGGCCGGCGCGG
                  ************************************************************

HsCD00002134      GAAGCCAGCCCTCGGCTCCGGGTCCACAAGACCTTCAAGTTTGTCGTCGTCGGGGTCCTG
NM_003844.3       GAAGCCAGCCCTCGGCTCCGGGTCCACAAGACCTTCAAGTTTGTCGTCGTCGGGGTCCTG
                  ************************************************************

HsCD00002134      CTGCAGGTCGTACCTAGCTCAGCTGCAACCATCAAACTTCATGATCAATCAATTGGCACA
NM_003844.3       CTGCAGGTCGTACCTAGCTCAGCTGCAACCATCAAACTTCATGATCAATCAATTGGCACA
                  ************************************************************

HsCD00002134      CAGCAATGGGAACATAGCCCTTTGGGAGAGTTGTGTCCACCAGGATCTCATAGATCAGAA
NM_003844.3       CAGCAATGGGAACATAGCCCTTTGGGAGAGTTGTGTCCACCAGGATCTCATAGATCAGAA
                  ************************************************************

HsCD00002134      CATCCTGGAGCCTGTAACCGGTGCACAGAGGGTGTGGGTTACACCAATGCTTCCAACAAT
NM_003844.3       CATCCTGGAGCCTGTAACCGGTGCACAGAGGGTGTGGGTTACACCAATGCTTCCAACAAT
                  ************************************************************

HsCD00002134      TTGTTTGCTTGCCTCCCATGTACAGCTTGTAAATCAGATGAAGAAGAGAGAAGTCCCTGC
NM_003844.3       TTGTTTGCTTGCCTCCCATGTACAGCTTGTAAATCAGATGAAGAAGAGAGAAGTCCCTGC
                  ************************************************************

HsCD00002134      ACCACGACCAGGAACACAGCATGTCAGTGCAAACCAGGAACTTTCCGGAATGACAATTCT
NM_003844.3       ACCACGACCAGGAACACAGCATGTCAGTGCAAACCAGGAACTTTCCGGAATGACAATTCT
                  ************************************************************

HsCD00002134      GCTGAGATGTGCCGGAAGTGCAGCAGAGGGTGCCCCAGAGGGATGGTCAAGGTCAAGGAT
NM_003844.3       GCTGAGATGTGCCGGAAGTGCAGCAGAGGGTGCCCCAGAGGGATGGTCAAGGTCAAGGAT
                  ************************************************************

HsCD00002134      TGTACGCCCTGGAGTGACATCGAGTGTGTCCACAAAGAATCAGGCAATGGACATAATATA
NM_003844.3       TGTACGCCCTGGAGTGACATCGAGTGTGTCCACAAAGAATCAGGCAATGGACATAATATA
                  ************************************************************

HsCD00002134      TGGGTGATTTTGGTTGTGACTTTGGTTGTTCCGTTGCTGTTGGTGGCTGTGCTGATTGTC
NM_003844.3       TGGGTGATTTTGGTTGTGACTTTGGTTGTTCCGTTGCTGTTGGTGGCTGTGCTGATTGTC
                  ************************************************************

HsCD00002134      TGTTGTTGCATCGGCTCAGGTTGTGGAGGGGACCCCAAGTGCATGGACAGGGTGTGTTTC
NM_003844.3       TGTTGTTGCATCGGCTCAGGTTGTGGAGGGGACCCCAAGTGCATGGACAGGGTGTGTTTC
                  ************************************************************

HsCD00002134      TGGCGCTTGGGTCTCCTACGAGGGCCTGGGGCTGAGGACAATGCTCACAACGAGATTCTG
NM_003844.3       TGGCGCTTGGGTCTCCTACGAGGGCCTGGGGCTGAGGACAATGCTCACAACGAGATTCTG
                  ************************************************************

HsCD00002134      AGCAACGCAGACTCGCTGTCCACTTTCGTCTCTGAGCAGCAAATGGAAAGCCAGGAGCCG
NM_003844.3       AGCAACGCAGACTCGCTGTCCACTTTCGTCTCTGAGCAGCAAATGGAAAGCCAGGAGCCG
                  ************************************************************

HsCD00002134      GCAGATTTGACAGGTGTCACTGTACAGTCCCCAGGGGAGGCACAGTGTCTGCTGGGACCG
NM_003844.3       GCAGATTTGACAGGTGTCACTGTACAGTCCCCAGGGGAGGCACAGTGTCTGCTGGGACCG
                  ************************************************************

HsCD00002134      GCAGAAGCTGAAGGGTCTCAGAGGAGGAGGCTGCTGGTTCCAGCAAATGGTGCTGACCCC
NM_003844.3       GCAGAAGCTGAAGGGTCTCAGAGGAGGAGGCTGCTGGTTCCAGCAAATGGTGCTGACCCC
                  ************************************************************

HsCD00002134      ACTGAGACTCTGATGCTGTTCTTTGACAAGTTTGCAAACATCGTGCCCTTTGACTCCTGG
NM_003844.3       ACTGAGACTCTGATGCTGTTCTTTGACAAGTTTGCAAACATCGTGCCCTTTGACTCCTGG
                  ************************************************************

HsCD00002134      GACCAGCTCATGAGGCAGCTGGACCTCACGAAAAATGAGATCGATGTGGTCAGAGCTGGT
NM_003844.3       GACCAGCTCATGAGGCAGCTGGACCTCACGAAAAATGAGATCGATGTGGTCAGAGCTGGT
                  ************************************************************

HsCD00002134      ACAGCAGGCCCAGGGGATGCCTTGTATGCAATGCTGATGAAATGGGTCAACAAAACTGGA
NM_003844.3       ACAGCAGGCCCAGGGGATGCCTTGTATGCAATGCTGATGAAATGGGTCAACAAAACTGGA
                  ************************************************************

HsCD00002134      CGGAACGCCTCGATCCACACCCTGCTGGATGCCTTGGAGAGGATGGAAGAGAGACATGCA
NM_003844.3       CGGAACGCCTCGATCCACACCCTGCTGGATGCCTTGGAGAGGATGGAAGAGAGACATGCA
                  ************************************************************

HsCD00002134      AAAGAGAAGATTCAGGACCTCTTGGTGGACTCTGGAAAGTTCATCTACTTAGAAGATGGC
NM_003844.3       AGAGAGAAGATTCAGGACCTCTTGGTGGACTCTGGAAAGTTCATCTACTTAGAAGATGGC
                  *.**********************************************************

HsCD00002134      ACAGGCTCTGCCGTGTCCTTGGAGTTG
NM_003844.3       ACAGGCTCTGCCGTGTCCTTGGAGTGA
                  ************************* .

Pathways

NCI : Caspase Cascade in Apoptosis
NCI : Direct p53 effectors
NCI : TRAIL signaling pathway
Panther : Apoptosis signaling pathway
Panther : p53 pathway
WikiPathway : Apoptosis Modulation and Signaling
WikiPathway : Lymphocyte TarBase

Clone

Cloning Information : 13799
HIP Master Clone ID : 27998
Original Clone ID : FLH013799.01L

Organism


TAX_ID : 9606
Species Specific ID: 8797

Genome

Chromosome : 8
Map Location : 8p21

Reagents

Labome : DR4-antibody