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TEC (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TEC
Gene Name:  tec protein tyrosine kinase
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH172436.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00021354

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1896nts         Open reading frame : 1 to 1896
Reference Sequence Alignment

ATGAATTTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTCACAGCAGAAAAAGAAG
ACATCGCCCTTAAACTACAAAGAGAGACTTTTTGTACTTACAAAGTCCATGCTAACCTAC
TATGAGGGTCGAGCAGAGAAGAAATACAGAAAGGGGTTTATTGATGTTTCAAAAATCAAG
TGTGTGGAAATAGTGAAGAATGATGATGGTGTCATTCCCTGTCAAAATAAGTATCCATTT
CAGGTTGTTCATGATGCTAACACACTTTACATTTTTGCACCTAGTCCACAAAGCAGGGAC
CTGTGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAATATTATGATTAAATAT
CATCCTAAATTCTGGACAGATGGAAGTTATCAGTGTTGTAGACAAACTGAAAAATTAGCA
CCCGGATGTGAAAAATACAATCTTTTTGAGAGTAGTATAAGAAAAGCACTACCTCCAGCA
CCAGAAACAAAGAAGCGAAGGCCTCCCCCACCAATTCCACTAGAAGAAGAAGATAATAGT
GAAGAAATCGTTGTAGCCATGTATGATTTCCAAGCAGCAGAAGGACATGATCTCAGATTA
GAGAGAGGCCAAGAGTATCTCATTTTAGAAAAGAATGATGTTCATTGGTGGAGAGCAAGA
GATAAATATGGGAATGAAGGATATATCCCAAGTAATTACGTAACGGGAAAGAAATCAAAC
AACTTAGATCAATATGAATGGTATTGCAGAAATATGAATAGAAGCAAGGCAGAGCAACTC
CTCCGCAGTGAAGATAAAGAAGGTGGTTTTATGGTAAGGGATTCCAGTCAACCAGGCTTG
TACACAGTCTCCCTTTATACCAAGTTTGGAGGAGAAGGTTCATCGGGTTTTAGGCATTAT
CATATAAAGGAAACAACAACATCTCCAAAGAAGTATTACCTAGCTGAAAAACATGCTTTT
GGCTCCATTCCTGAGATTATTGAATATCATAAGCACAATGCAGCAGGACTTGTCACCAGG
CTTCGGTACCCAGTTAGTGTGAAAGGGAAGAATGCACCCACCACTGCAGGATTCAGCTAT
GAGAAATGGGAGATTAACCCTTCAGAACTGACCTTTATGAGGGAATTGGGAAGTGGACTG
TTTGGAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACAAAGTCGCAATCAAAGCTATT
CGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGTGATGATGAAACTG
ACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAAACCAATATACATT
GTTACTGAGTTCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGACAGAGACAAGGTCAT
TTCAGTAGAGACGTACTGCTGAGCATGTGTCAGGATGTGTGTGAAGGGATGGAGTATCTG
GAGAGAAACAGCTTCATCCACAGAGATCTGGCTGCCAGAAATTGTCTAGTAAGTGAGGCG
GGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATGTTCTGGATGATCAGTACACA
AGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGTGTCCACCTGAAGTGTTTAATTACAGC
CGCTTCAGCAGCAAATCAGATGTCTGGTCATTTGGTGTTTTAATGTGGGAAGTATTCACG
GAAGGCAGAATGCCTTTTGAAAAATACACCAATTATGAAGTGGTAACCATGGTTACTCGA
GGCCACCGACTCTACCAGCCGAAGTTGGCGTCCAACTATGTGTATGAGGTGATGCTGAGA
TGTTGGCAGGAGAAACCAGAGGGAAGGCCTTCTTTCGAAGATCTGCTGCGCACAATAGAT
GAACTAGTTGAATGTGAAGAAACTTTTGGAAGATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2013
Start on reference sequence 118
Mutations:
None
Discrepancy:
g39a; c453t;
 g642t; g1257a;
 t1540g,F514V
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TEC
Symbol Nomenclature : TEC
SYNONYM : PSCTK4
Designation : tyrosine-protein kinase Tec
Full Nomenclature : tec protein tyrosine kinase
GENEID : 7006
HGNC : 11719
MIM : 600583
Vega : OTTHUMG00000128623
Target GenBank: NM_003215

 

Reference Sequence Alignment

  

HsCD00021354      ATGAATTTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTCACAGCAGAAAAAGAAG
NM_003215.2       ATGAATTTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTCACAGCAGAAAAAGAAG
                  ************************************************************

HsCD00021354      ACATCGCCCTTAAACTACAAAGAGAGACTTTTTGTACTTACAAAGTCCATGCTAACCTAC
NM_003215.2       ACATCGCCCTTAAACTACAAAGAGAGACTTTTTGTACTTACAAAGTCCATGCTAACCTAC
                  ************************************************************

HsCD00021354      TATGAGGGTCGAGCAGAGAAGAAATACAGAAAGGGGTTTATTGATGTTTCAAAAATCAAG
NM_003215.2       TATGAGGGTCGAGCAGAGAAGAAATACAGAAAGGGGTTTATTGATGTTTCAAAAATCAAG
                  ************************************************************

HsCD00021354      TGTGTGGAAATAGTGAAGAATGATGATGGTGTCATTCCCTGTCAAAATAAGTATCCATTT
NM_003215.2       TGTGTGGAAATAGTGAAGAATGATGATGGTGTCATTCCCTGTCAAAATAAGTATCCATTT
                  ************************************************************

HsCD00021354      CAGGTTGTTCATGATGCTAACACACTTTACATTTTTGCACCTAGTCCACAAAGCAGGGAC
NM_003215.2       CAGGTTGTTCATGATGCTAACACACTTTACATTTTTGCACCTAGTCCACAAAGCAGGGAC
                  ************************************************************

HsCD00021354      CTGTGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAATATTATGATTAAATAT
NM_003215.2       CTGTGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAATATTATGATTAAATAT
                  ************************************************************

HsCD00021354      CATCCTAAATTCTGGACAGATGGAAGTTATCAGTGTTGTAGACAAACTGAAAAATTAGCA
NM_003215.2       CATCCTAAATTCTGGACAGATGGAAGTTATCAGTGTTGTAGACAAACTGAAAAATTAGCA
                  ************************************************************

HsCD00021354      CCCGGATGTGAAAAATACAATCTTTTTGAGAGTAGTATAAGAAAAGCACTACCTCCAGCA
NM_003215.2       CCCGGATGTGAAAAATACAATCTTTTTGAGAGCAGTATAAGAAAAGCACTACCTCCAGCA
                  ******************************** ***************************

HsCD00021354      CCAGAAACAAAGAAGCGAAGGCCTCCCCCACCAATTCCACTAGAAGAAGAAGATAATAGT
NM_003215.2       CCAGAAACAAAGAAGCGAAGGCCTCCCCCACCAATTCCACTAGAAGAAGAAGATAATAGT
                  ************************************************************

HsCD00021354      GAAGAAATCGTTGTAGCCATGTATGATTTCCAAGCAGCAGAAGGACATGATCTCAGATTA
NM_003215.2       GAAGAAATCGTTGTAGCCATGTATGATTTCCAAGCAGCAGAAGGACATGATCTCAGATTA
                  ************************************************************

HsCD00021354      GAGAGAGGCCAAGAGTATCTCATTTTAGAAAAGAATGATGTTCATTGGTGGAGAGCAAGA
NM_003215.2       GAGAGAGGCCAAGAGTATCTCATTTTAGAAAAGAATGATGTTCATTGGTGGAGAGCAAGA
                  ************************************************************

HsCD00021354      GATAAATATGGGAATGAAGGATATATCCCAAGTAATTACGTAACGGGAAAGAAATCAAAC
NM_003215.2       GATAAATATGGGAATGAAGGATATATCCCAAGTAATTACGTAACGGGAAAGAAATCAAAC
                  ************************************************************

HsCD00021354      AACTTAGATCAATATGAATGGTATTGCAGAAATATGAATAGAAGCAAGGCAGAGCAACTC
NM_003215.2       AACTTAGATCAATATGAATGGTATTGCAGAAATATGAATAGAAGCAAGGCAGAGCAACTC
                  ************************************************************

HsCD00021354      CTCCGCAGTGAAGATAAAGAAGGTGGTTTTATGGTAAGGGATTCCAGTCAACCAGGCTTG
NM_003215.2       CTCCGCAGTGAAGATAAAGAAGGTGGTTTTATGGTAAGGGATTCCAGTCAACCAGGCTTG
                  ************************************************************

HsCD00021354      TACACAGTCTCCCTTTATACCAAGTTTGGAGGAGAAGGTTCATCGGGTTTTAGGCATTAT
NM_003215.2       TACACAGTCTCCCTTTATACCAAGTTTGGAGGAGAAGGTTCATCGGGTTTTAGGCATTAT
                  ************************************************************

HsCD00021354      CATATAAAGGAAACAACAACATCTCCAAAGAAGTATTACCTAGCTGAAAAACATGCTTTT
NM_003215.2       CATATAAAGGAAACAACAACATCTCCAAAGAAGTATTACCTAGCTGAAAAACATGCTTTT
                  ************************************************************

HsCD00021354      GGCTCCATTCCTGAGATTATTGAATATCATAAGCACAATGCAGCAGGACTTGTCACCAGG
NM_003215.2       GGCTCCATTCCTGAGATTATTGAATATCATAAGCACAATGCAGCAGGACTTGTCACCAGG
                  ************************************************************

HsCD00021354      CTTCGGTACCCAGTTAGTGTGAAAGGGAAGAATGCACCCACCACTGCAGGATTCAGCTAT
NM_003215.2       CTTCGGTACCCAGTTAGTGTGAAAGGGAAGAATGCACCCACCACTGCAGGATTCAGCTAT
                  ************************************************************

HsCD00021354      GAGAAATGGGAGATTAACCCTTCAGAACTGACCTTTATGAGGGAATTGGGAAGTGGACTG
NM_003215.2       GAGAAATGGGAGATTAACCCTTCAGAACTGACCTTTATGAGGGAATTGGGAAGTGGACTG
                  ************************************************************

HsCD00021354      TTTGGAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACAAAGTCGCAATCAAAGCTATT
NM_003215.2       TTTGGAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACAAAGTCGCAATCAAAGCTATT
                  ************************************************************

HsCD00021354      CGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGTGATGATGAAACTG
NM_003215.2       CGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGTGATGATGAAACTG
                  ************************************************************

HsCD00021354      ACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAAACCAATATACATT
NM_003215.2       ACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAAACCAATATACATT
                  ************************************************************

HsCD00021354      GTTACTGAGTTCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGACAGAGACAAGGTCAT
NM_003215.2       GTTACTGAGTTCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGACAGAGACAAGGTCAT
                  ************************************************************

HsCD00021354      TTCAGTAGAGACGTACTGCTGAGCATGTGTCAGGATGTGTGTGAAGGGATGGAGTATCTG
NM_003215.2       TTCAGTAGAGACGTACTGCTGAGCATGTGTCAGGATGTGTGTGAAGGGATGGAGTATCTG
                  ************************************************************

HsCD00021354      GAGAGAAACAGCTTCATCCACAGAGATCTGGCTGCCAGAAATTGTCTAGTAAGTGAGGCG
NM_003215.2       GAGAGAAACAGCTTCATCCACAGAGATCTGGCTGCCAGAAATTGTCTAGTAAGTGAGGCG
                  ************************************************************

HsCD00021354      GGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATGTTCTGGATGATCAGTACACA
NM_003215.2       GGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATGTTCTGGATGATCAGTACACA
                  ************************************************************

HsCD00021354      AGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGTGTCCACCTGAAGTGTTTAATTACAGC
NM_003215.2       AGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGTGTCCACCTGAAGTGTTTAATTACAGC
                  ************************************************************

HsCD00021354      CGCTTCAGCAGCAAATCAGATGTCTGGTCATTTGGTGTTTTAATGTGGGAAGTATTCACG
NM_003215.2       CGCTTCAGCAGCAAATCAGATGTCTGGTCATTTGGTGTTTTAATGTGGGAAGTATTCACG
                  ************************************************************

HsCD00021354      GAAGGCAGAATGCCTTTTGAAAAATACACCAATTATGAAGTGGTAACCATGGTTACTCGA
NM_003215.2       GAAGGCAGAATGCCTTTTGAAAAATACACCAATTATGAAGTGGTAACCATGGTTACTCGA
                  ************************************************************

HsCD00021354      GGCCACCGACTCTACCAGCCGAAGTTGGCGTCCAACTATGTGTATGAGGTGATGCTGAGA
NM_003215.2       GGCCACCGACTCTACCAGCCGAAGTTGGCGTCCAACTATGTGTATGAGGTGATGCTGAGA
                  ************************************************************

HsCD00021354      TGTTGGCAGGAGAAACCAGAGGGAAGGCCTTCTTTCGAAGATCTGCTGCGCACAATAGAT
NM_003215.2       TGTTGGCAGGAGAAACCAGAGGGAAGGCCTTCTTTCGAAGATCTGCTGCGCACAATAGAT
                  ************************************************************

HsCD00021354      GAACTAGTTGAATGTGAAGAAACTTTTGGAAGATAG
NM_003215.2       GAACTAGTTGAATGTGAAGAAACTTTTGGAAGATAA
                  ***********************************.

Pathways

NCI : EPO signaling pathway
NCI : Signaling events mediated by Stem cell factor receptor (c-Kit)
WikiPathway : B Cell Receptor Signaling Pathway
WikiPathway : Kit receptor signaling pathway
WikiPathway : Prolactin Signaling Pathway

Clone

Cloning Information : 172436
HIP Master Clone ID : 146633
Original Clone ID : FLH172436.01X

Organism


TAX_ID : 9606
Species Specific ID: 7006

Genome

Chromosome : 4
Map Location : 4p12
Ensembl : ENSG00000135605

Reagents

Labome : TEC-antibody