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UCK1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  UCK1
Gene Name:  uridine-cytidine kinase 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH172515.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00021371

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 606nts         Open reading frame : 1 to 606
Reference Sequence Alignment

ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
CAGCGGCCCTTCCTGATAGGGGTGAGCGGCGGCACTGCCAGCGGGAAGTCGACCGTGTGT
GAGAAGATCATGGAGTTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTC
ATCCTGAGCCAGGACAGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTG
AAAGGACAGTACAATTTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACT
CTGAAGAACATCGTGGAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACAC
TCAAGGTTACCAGAGACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATC
TTGGTGTTCTACAGCCAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACC
GACTCCGACGTCAGGCTGTCTCGAAGAGACAAAGAAGTATGCCGATGTGATCATCCCACG
AGGAGTGGACAATATGGTTGCCATCAACCTGATCGTGCAGCACATCCAGGACATTCTGAA
TGGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 692
Start on reference sequence 87
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UCK1
Symbol Nomenclature : UCK1
SYNONYM : URK1
Designation : UCK 1|cytidine monophosphokinase 1|uridine monophosphokinase 1
Full Nomenclature : uridine-cytidine kinase 1
GENEID : 83549
Locus Tag : RP11-334J6.5
HGNC : 14859
MIM : 609328
Vega : OTTHUMG00000020823
Target GenBank: BC015547

 

Reference Sequence Alignment

  

HsCD00021371        ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
NM_001261451.1      ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
XM_005272224.1      ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
XM_005272225.1      ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
NM_001261450.1      ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
NM_001135954.1      ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
NM_031432.2         ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
NM_012474.4         ------------ATGGCCGGGGACAGCGAGCAGACCCTGCAGAACCACCAGCAGCCCAAC
                                . .* **..***:*****.. .**  **.*.*  .* * *. ** .**

HsCD00021371        CAG---CGGCCCTTCCTGATAGGGGTGAGCGGCGGCACTGCCAGCGGG------------
NM_001261451.1      CAG---CGGCCCTTCCTGATAGGGGACGAGCGGTTCCAGGCGGGAATCCCG---------
XM_005272224.1      CAG---CGGCCCTTCCTGATAGGGGTGAGCGGCGGCACTGCCAGCGGGAAGGACGAGCGG
XM_005272225.1      CAG---CGGCCCTTCCTGATAGGGGTGAGCGGCGGCACTGCCAGCGGG------------
NM_001261450.1      CAG---CGGCCCTTCCTGATAGGG------------------------------------
NM_001135954.1      CAG---CGGCCCTTCCTGATAGGGGTGAGCGGCGGCACTGCCAGCGGG------------
NM_031432.2         CAG---CGGCCCTTCCTGATAGGGGTGAGCGGCGGCACTGCCAGCGGG------------
NM_012474.4         GGCGGCGAGCCCTTCCTTATAGGCGTCAGCGGGGGAACAGCTAGCGGCAAG---------
                     .     .********* *****                                     

HsCD00021371        ------------------------------AAGTCGACCGTGTGTGAGAAGATCATGGAG
NM_001261451.1      ------------------CTTCTGTGTCTCCAGTCGACCGTGTGTGAGAAGATCATGGAG
XM_005272224.1      TTCCAGGCGGGAATCCCGCTTCTGTGTCTCCAGTCGACCGTGTGTGAGAAGATCATGGAG
XM_005272225.1      ------------------------------AAGTCGACCGTGTGTGAGAAGATCATGGAG
NM_001261450.1      ---------------------------------TCGACCGTGTGTGAGAAGATCATGGAG
NM_001135954.1      ------------------------------AAGTCGACCGTGTGTGAGAAGATCATGGAG
NM_031432.2         ------------------------------AAGTCGACCGTGTGTGAGAAGATCATGGAG
NM_012474.4         ---------------------------------TCTTCCGTGTGTGCTAAGATCGTGCAG
                                                     ** :*********. ******.** **

HsCD00021371        TTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGAC
NM_001261451.1      TTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGAC
XM_005272224.1      TTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGAC
XM_005272225.1      TTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGAC
NM_001261450.1      TTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGAC
NM_001135954.1      TTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGAC
NM_031432.2         TTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGAC
NM_012474.4         CTCCTGGGGCAGAATGAGGTGGACTATCGCCAGAAGCAGGTGGTCATCCTGAGCCAGGAT
                     * *****.***** ********. * ** ***..*.********************** 

HsCD00021371        AGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAAT
NM_001261451.1      AGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAAT
XM_005272224.1      AGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAAT
XM_005272225.1      AGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAAT
NM_001261450.1      AGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAAT
NM_001135954.1      AGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAAT
NM_031432.2         AGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAAT
NM_012474.4         AGCTTCTACCGTGTCCTTACCTCGGAGCAGAAGGCCAAAGCCCTGAAGGGCCAGTTCAAC
                    ** ******.. ***** **  *.**************.*** ****.**.****:*** 

HsCD00021371        TTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTG
NM_001261451.1      TTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTG
XM_005272224.1      TTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTG
XM_005272225.1      TTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTG
NM_001261450.1      TTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTG
NM_001135954.1      TTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTG
NM_031432.2         TTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTG
NM_012474.4         TTTGACCACCCGGATGCCTTTGACAATGAACTCATTCTCAAAACACTCAAAGAAATCACT
                    ******** **.*********** *****: * ** *:**..**:** **..*.***.  

HsCD00021371        GAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACACTCAAGGTTACCAGAG
NM_001261451.1      GAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACACTCAAGGTTACCAGAG
XM_005272224.1      GAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACACTCAAGGTTACCAGAG
XM_005272225.1      GAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACACTCAAG----------
NM_001261450.1      GAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACACTCAAGGTTACCAGAG
NM_001135954.1      GAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACACTCAAGGTTACCAGAG
NM_031432.2         GAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACACTCAAGGTTACCAGAG
NM_012474.4         GAAGGGAAAACAGTCCAGATCCCCGTGTATGACTTTGTCTCCCATTCCCGGAAGGAGGAG
                    **.** *****.**  **.* ** .  ***** ***** :*.** **..*          

HsCD00021371        ACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATCTTGGTGTTCTACAGC
NM_001261451.1      ACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATCTTGGTGTTCTACAGC
XM_005272224.1      ACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATCTTGGTGTTCTACAGC
XM_005272225.1      -----------------------------------------------------------C
NM_001261450.1      ACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATCTTGGTGTTCTACAGC
NM_001135954.1      ACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATCTTGGTGTTCTACAGC
NM_031432.2         ACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATCTTGGTGTTCTACAGC
NM_012474.4         ACAGTTACTGTCTATCCCGCAGACGTGGTGCTCTTTGAAGGGATCCTGGCCTTCTACTCC
                                                                               *

HsCD00021371        CAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACGTCAGG
NM_001261451.1      CAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACGTCAGG
XM_005272224.1      CAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACGTCAGG
XM_005272225.1      CAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACGTCAGG
NM_001261450.1      CAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACGTCAGG
NM_001135954.1      CAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACGTCAGG
NM_031432.2         CAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACGTCAGG
NM_012474.4         CAGGAGGTACGAGACCTGTTCCAGATGAAGCTTTTTGTGGATACAGATGCGGACACCCGG
                    ******.*.**.***.******* .**.. ** ** ***** **.**  * ***. *.**

HsCD00021371        CTGTCTCGAAGAG-----------------------------------------------
NM_001261451.1      CTGTCTCGAAGAGTTCTCCGGGACGTGCGC---CGAGGGAGGGACCTGGAGCAGATTCTG
XM_005272224.1      CTGTCTCGAAGAGTTCTCCGGGACGTGCGC---CGAGGGAGGGACCTGGAGCAGATTCTG
XM_005272225.1      CTGTCTCGAAGAGTTCTCCGGGACGTGCGC---CGAGGGAGGGACCTGGAGCAGATTCTG
NM_001261450.1      CTGTCTCGAAGAGTTCTCCGGGACGTGCGC---CGAGGGAGGGACCTGGAGCAGATTCTG
NM_001135954.1      CTGTCTCGAAGAG-----------------------------------------------
NM_031432.2         CTGTCTCGAAGAGTTCTCCGGGACGTGCGC---CGAGGGAGGGACCTGGAGCAGATTCTG
NM_012474.4         CTCTCACGCAGAGTATTAAGGGACATCAGCGAGAGAGGCAGGGATCTTGAGCAGATTTTA
                    ** **:**.****                                               

HsCD00021371        ---------------------------------------------------ACAAAGAAG
NM_001261451.1      ACGCAGTACACCACCTTCGTGAAGCCGGCCTTCGAGGAGTTCTGCCTGCCGACAAAGAAG
XM_005272224.1      ACGCAGTACACCACCTTCGTGAAGCCGGCCTTCGAGGAGTTCTGCCTGCCGACAAAGAAG
XM_005272225.1      ACGCAGTACACCACCTTCGTGAAGCCGGCCTTCGAGGAGTTCTGCCTGCCGACAAAGAAG
NM_001261450.1      ACGCAGTACACCACCTTCGTGAAGCCGGCCTTCGAGGAGTTCTGCCTGCCGACAAAGAAG
NM_001135954.1      ---------------------------------------------------ACAAAGAAG
NM_031432.2         ACGCAGTACACCACCTTCGTGAAGCCGGCCTTCGAGGAGTTCTGCCTGCCGACAAAGAAG
NM_012474.4         TCTCAGTACATTACGTTCGTCAAGCCTGCCTTTGAGGAATTCTGCTTGCCAACAAAGAAG
                                                                       *********

HsCD00021371        TATGCCGATGTGATCATCCCACGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTG
NM_001261451.1      TATGCCGATGTGATCATCCCGCGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTG
XM_005272224.1      TATGCCGATGTGATCATCCCGCGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTG
XM_005272225.1      TATGCCGATGTGATCATCCCGCGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTG
NM_001261450.1      TATGCCGATGTGATCATCCCGCGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTG
NM_001135954.1      TATGCCGATGTGATCATCCCGCGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTG
NM_031432.2         TATGCCGATGTGATCATCCCGCGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTG
NM_012474.4         TATGCTGATGTGATCATCCCTAGAGGTGCAGATAATCTGGTGGCCATCAACCTCATCGTG
                    ***** ************** .****:* .** ***.**** *********** ******

HsCD00021371        CAGCACATCCAGGACATTCTGAATGGTAG-------------------------------
NM_001261451.1      CAGCACATCCAGGACATTCTGAATGGTGACATCTGCAAATGGCACCGAGGAGGGTCCAAT
XM_005272224.1      CAGCACATCCAGGACATTCTGAATGGTGACATCTGCAAATGGCACCGAGGAGGGTCCAAT
XM_005272225.1      CAGCACATCCAGGACATTCTGAATGGTGACATCTGCAAATGGCACCGAGGAGGGTCCAAT
NM_001261450.1      CAGCACATCCAGGACATTCTGAATGGTGACATCTGCAAATGGCACCGAGGAGGGTCCAAT
NM_001135954.1      CAGCACATCCAGGACATTCTGAATGGTGA-------------------------------
NM_031432.2         CAGCACATCCAGGACATTCTGAATGGTGACATCTGCAAATGGCACCGAGGAGGGTCCAAT
NM_012474.4         CAGCACATCCAGGACATCCTGAATGGAGGGCCCTCCAAACGGCAGACCAATGG-------
                    ***************** ********:..                               

HsCD00021371        ------------------------------------------------------------
NM_001261451.1      GGGCGGAGCTACAAGCGGACCTTTTCTGAGCCAGGGGACCACCCTGGGATGCTGACCTCT
XM_005272224.1      GGGCGGAGCTACAAGCGGACCTTTTCTGAGCCAGGGGACCACCCTGGGATGCTGACCTCT
XM_005272225.1      GGGCGGAGCTACAAGCGGACCTTTTCTGAGCCAGGGGACCACCCTGGGATGCTGACCTCT
NM_001261450.1      GGGCGGAGCTACAAGCGGACCTTTTCTGAGCCAGGGGACCACCCTGGGATGCTGACCTCT
NM_001135954.1      ------------------------------------------------------------
NM_031432.2         GGGCGGAGCTACAAGCGGACCTTTTCTGAGCCAGGGGACCACCCTGGGATGCTGACCTCT
NM_012474.4         -----------------------------------CTGTCTCAACGGCTACACCCCTTCA
                                                                                

HsCD00021371        ------------------------------------------
NM_001261451.1      GGCAAACGGTCACATTTGGAGTCCAGCAGCAGACCCCACTGA
XM_005272224.1      GGCAAACGGTCACATTTGGAGTCCAGCAGCAGACCCCACTGA
XM_005272225.1      GGCAAACGGTCACATTTGGAGTCCAGCAGCAGACCCCACTGA
NM_001261450.1      GGCAAACGGTCACATTTGGAGTCCAGCAGCAGACCCCACTGA
NM_001135954.1      ------------------------------------------
NM_031432.2         GGCAAACGGTCACATTTGGAGTCCAGCAGCAGACCCCACTGA
NM_012474.4         CGCAAGAGGCAGGCATCGGAGTCCAGCAGCAGGCCGCATTGA
                                                              

Pathways

Panther : Salvage pyrimidine ribonucleotides
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Pyrimidine metabolism
Reactome : Pyrimidine salvage reactions
WikiPathway : Fluoropyrimidine Activity

Protein

UniProt : Q9HA47
HPRD : 18256

Clone

Cloning Information : 172515
HIP Master Clone ID : 146712
Original Clone ID : FLH172515.01X

Organism


TAX_ID : 9606
Species Specific ID: 83549

Genome

Chromosome : 9
Map Location : 9q34.13
Ensembl : ENSG00000130717

Reagents

Labome : UCK1-antibody