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EPHA10 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  EPHA10
Gene Name:  EPH receptor A10
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH204043.01F
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors: HIP
HsCD00021533

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 888nts         Open reading frame : 1 to 888
Reference Sequence Alignment

ATGGAGACCTGCGCCGGTCCACACCCGCTGCGCCTCTTCCTCTGCCGGATGCAGCTCTGT
CTCGCGCTGCTTTTGGGACCCTGGCGGCCTGGGACCGCCGAGGAAGTTATCCTCCTGGAT
TCCAAAGCCTCCCAGGCCGAGCTGGGCTGGACTGCACTGCCAAGTAATGGGTGGGAGGAG
ATCAGCGGCGTGGATGAACACGACCGTCCCATCCGCACGTACCAAGTGTGCAATGTGCTG
GAGCCCAACCAGGACAACTGGCTGCAGACTGGCTGGATAAGCCGTGGCCGCGGGCAGCGC
ATCTTCGTGGAACTGCAGTTCACACTCCGTGACTGCAGCAGCATCCCTGGCGCCGCGGGT
ACCTGCAAGGAGACCTTCAACGTCTACTACCTGGAAACTGAGGCCGACCTGGGCCGTGGG
CGTCCCCGCCTAGGCGGCAGCCGGCCCCGCAAAATCGACACGATCGCGGCGGACGAGAGC
TTCACGCAGGGCGACCTGGGTGAGCGCAAGATGAAGCTGAACACAGAGGTGCGCGAGATC
GGACCGCTCAGCCGGCGGGGTTTCCACCTGGCCTTTCAGGACGTGGGCGCATGCGTGGCG
CTTGTCTCGGTGCGCGTCTACTACAAGCAGTGCCGCGCCACCGTGCGGGGCCTGGCCACG
TTCCCAGCCACCGCAGCCGAGAGCGCCTTCTCCACACTGGTGGAAGTGGCCGGAACGTGC
GTGGCGCACTCGGAAGGGGAGCCTGGCAGCCCCCCACGCATGCACTGCGGCGCCGACGGC
GAGTGGCTGGTGCCTGTGGGCCGCTGCAGCTGCAGCGCGGGATTCCAGGAGCGTGGTGAC
TTCTGCGAAGGTATCCAGTTGGCTGGGGGCCGTGGGGTGGGAGTGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 974
Start on reference sequence 87
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : EPHA10
Symbol Nomenclature : EPHA10
Designation : EphA10s protein|ephrin type-A receptor 10
Full Nomenclature : EPH receptor A10
GENEID : 284656
HGNC : 19987
MIM : 611123
Vega : OTTHUMG00000004325
Target GenBank: BC067734

 

Reference Sequence Alignment

  

HsCD00021533        ATGGAGACCTGCGCCGGTCCACACCCGCTGCGCCTCTTCCTCTGCCGGATGCAGCTCTGT
NM_001099439.1      ATGGAGACCTGCGCCGGTCCACACCCGCTGCGCCTCTTCCTCTGCCGGATGCAGCTCTGT
NM_173641.2         ATGGAGACCTGCGCCGGTCCACACCCGCTGCGCCTCTTCCTCTGCCGGATGCAGCTCTGT
                    ************************************************************

HsCD00021533        CTCGCGCTGCTTTTGGGACCCTGGCGGCCTGGGACCGCCGAGGAAGTTATCCTCCTGGAT
NM_001099439.1      CTCGCGCTGCTTTTGGGACCCTGGCGGCCTGGGACCGCCGAGGAAGTTATCCTCCTGGAT
NM_173641.2         CTCGCGCTGCTTTTGGGACCCTGGCGGCCTGGGACCGCCGAGGAAGTTATCCTCCTGGAT
                    ************************************************************

HsCD00021533        TCCAAAGCCTCCCAGGCCGAGCTGGGCTGGACTGCACTGCCAAGTAATGGGTGGGAGGAG
NM_001099439.1      TCCAAAGCCTCCCAGGCCGAGCTGGGCTGGACTGCACTGCCAAGTAATGGGTGGGAGGAG
NM_173641.2         TCCAAAGCCTCCCAGGCCGAGCTGGGCTGGACTGCACTGCCAAGTAATGGGTGGGAGGAG
                    ************************************************************

HsCD00021533        ATCAGCGGCGTGGATGAACACGACCGTCCCATCCGCACGTACCAAGTGTGCAATGTGCTG
NM_001099439.1      ATCAGCGGCGTGGATGAACACGACCGTCCCATCCGCACGTACCAAGTGTGCAATGTGCTG
NM_173641.2         ATCAGCGGCGTGGATGAACACGACCGTCCCATCCGCACGTACCAAGTGTGCAATGTGCTG
                    ************************************************************

HsCD00021533        GAGCCCAACCAGGACAACTGGCTGCAGACTGGCTGGATAAGCCGTGGCCGCGGGCAGCGC
NM_001099439.1      GAGCCCAACCAGGACAACTGGCTGCAGACTGGCTGGATAAGCCGTGGCCGCGGGCAGCGC
NM_173641.2         GAGCCCAACCAGGACAACTGGCTGCAGACTGGCTGGATAAGCCGTGGCCGCGGGCAGCGC
                    ************************************************************

HsCD00021533        ATCTTCGTGGAACTGCAGTTCACACTCCGTGACTGCAGCAGCATCCCTGGCGCCGCGGGT
NM_001099439.1      ATCTTCGTGGAACTGCAGTTCACACTCCGTGACTGCAGCAGCATCCCTGGCGCCGCGGGT
NM_173641.2         ATCTTCGTGGAACTGCAGTTCACACTCCGTGACTGCAGCAGCATCCCTGGCGCCGCGGGT
                    ************************************************************

HsCD00021533        ACCTGCAAGGAGACCTTCAACGTCTACTACCTGGAAACTGAGGCCGACCTGGGCCGTGGG
NM_001099439.1      ACCTGCAAGGAGACCTTCAACGTCTACTACCTGGAAACTGAGGCCGACCTGGGCCGTGGG
NM_173641.2         ACCTGCAAGGAGACCTTCAACGTCTACTACCTGGAAACTGAGGCCGACCTGGGCCGTGGG
                    ************************************************************

HsCD00021533        CGTCCCCGCCTAGGCGGCAGCCGGCCCCGCAAAATCGACACGATCGCGGCGGACGAGAGC
NM_001099439.1      CGTCCCCGCCTAGGCGGCAGCCGGCCCCGCAAAATCGACACGATCGCGGCGGACGAGAGC
NM_173641.2         CGTCCCCGCCTAGGCGGCAGCCGGCCCCGCAAAATCGACACGATCGCGGCGGACGAGAGC
                    ************************************************************

HsCD00021533        TTCACGCAGGGCGACCTGGGTGAGCGCAAGATGAAGCTGAACACAGAGGTGCGCGAGATC
NM_001099439.1      TTCACGCAGGGCGACCTGGGTGAGCGCAAGATGAAGCTGAACACAGAGGTGCGCGAGATC
NM_173641.2         TTCACGCAGGGCGACCTGGGTGAGCGCAAGATGAAGCTGAACACAGAGGTGCGCGAGATC
                    ************************************************************

HsCD00021533        GGACCGCTCAGCCGGCGGGGTTTCCACCTGGCCTTTCAGGACGTGGGCGCATGCGTGGCG
NM_001099439.1      GGACCGCTCAGCCGGCGGGGTTTCCACCTGGCCTTTCAGGACGTGGGCGCATGCGTGGCG
NM_173641.2         GGACCGCTCAGCCGGCGGGGTTTCCACCTGGCCTTTCAGGACGTGGGCGCATGCGTGGCG
                    ************************************************************

HsCD00021533        CTTGTCTCGGTGCGCGTCTACTACAAGCAGTGCCGCGCCACCGTGCGGGGCCTGGCCACG
NM_001099439.1      CTTGTCTCGGTGCGCGTCTACTACAAGCAGTGCCGCGCCACCGTGCGGGGCCTGGCCACG
NM_173641.2         CTTGTCTCGGTGCGCGTCTACTACAAGCAGTGCCGCGCCACCGTGCGGGGCCTGGCCACG
                    ************************************************************

HsCD00021533        TTCCCAGCCACCGCAGCCGAGAGCGCCTTCTCCACACTGGTGGAAGTGGCCGGAACGTGC
NM_001099439.1      TTCCCAGCCACCGCAGCCGAGAGCGCCTTCTCCACACTGGTGGAAGTGGCCGGAACGTGC
NM_173641.2         TTCCCAGCCACCGCAGCCGAGAGCGCCTTCTCCACACTGGTGGAAGTGGCCGGAACGTGC
                    ************************************************************

HsCD00021533        GTGGCGCACTCGGAAGGGGAGCCTGGCAGCCCCCCACGCATGCACTGCGGCGCCGACGGC
NM_001099439.1      GTGGCGCACTCGGAAGGGGAGCCTGGCAGCCCCCCACGCATGCACTGCGGCGCCGACGGC
NM_173641.2         GTGGCGCACTCGGAAGGGGAGCCTGGCAGCCCCCCACGCATGCACTGCGGCGCCGACGGC
                    ************************************************************

HsCD00021533        GAGTGGCTGGTGCCTGTGGGCCGCTGCAGCTGCAGCGCGGGATTCCAGGAGCGTGGTGAC
NM_001099439.1      GAGTGGCTGGTGCCTGTGGGCCGCTGCAGCTGCAGCGCGGGATTCCAGGAGCGTGGTGAC
NM_173641.2         GAGTGGCTGGTGCCTGTGGGCCGCTGCAGCTGCAGCGCGGGATTCCAGGAGCGTGGTGAC
                    ************************************************************

HsCD00021533        TTCTGCGAAGGTATCCAGTTGGCTGGGGGCCGTGGGGTGGGAGTGTTG------------
NM_001099439.1      TTCTGCGAAGCCTGTCCCCCAGGGTTTTACAAGGTGTCCCCGCGGCGGCCCCTCTGCTCA
NM_173641.2         TTCTGCGAAGGTATCCAGTTGGCTGGGGGCCGTGGGGTGGGAGTGTAG------------
                    **********  :  *.   .*      .*.. * *     .  *  *            

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      CCGTGCCCAGAGCACAGCCGGGCCCTGGAAAACGCCTCCACCTTCTGCGTGTGCCAGGAC
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      AGCTATGCGCGCTCACCCACCGACCCGCCCTCGGCTTCCTGCACCCGGCCGCCGTCGGCG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      CCGCGGGACCTGCAGTACAGCCTGAGCCGCTCGCCGCTGGTGCTGCGACTGCGCTGGCTG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      CCGCCGGCCGACTCGGGAGGCCGCTCGGACGTCACCTACTCGCTGCTGTGCCTGCGCTGC
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      GGCCGCGAGGGCCCGGCGGGCGCCTGCGAGCCGTGCGGGCCGCGCGTGGCCTTCCTACCG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      CGCCAGGCAGGGCTGCGGGAGCGAGCCGCCACGCTGCTGCACCTGCGGCCCGGCGCGCGC
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      TACACCGTGCGCGTGGCCGCGCTCAACGGCGTCTCGGGCCCGGCGGCCGCCGCGGGAACC
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      ACCTACGCGCAGGTCACCGTCTCCACCGGGCCCGGGGCGCCCTGGGAGGAGGATGAGATC
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      CGCAGGGACCGAGTGGAACCCCAGAGCGTGTCCCTGTCGTGGCGGGAGCCCATCCCTGCC
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      GGAGCCCCTGGGGCCAATGACACGGAGTACGAGATCCGATACTACGAGAAGGGTCAGAGT
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      GAGCAGACTTACTCCATGGTGAAGACAGGGGCGCCCACAGTCACCGTCACCAACCTGAAG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      CCGGCTACCCGCTACGTCTTTCAGATCCGGGCCGCTTCCCCGGGGCCATCCTGGGAGGCC
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      CAGAGTTTTAACCCCAGCATTGAAGTACAGACCCTGGGGGAGGCTGCCTCAGGGTCCAGG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      GACCAGAGCCCCGCCATTGTCGTCACCGTAGTGACCATCTCGGCCCTCCTCGTCCTGGGC
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      TCCGTGATGAGTGTGCTGGCCATTTGGAGGAGGCCCTGCAGCTATGGCAAAGGAGGAGGG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      GATGCCCATGATGAAGAGGAGCTGTATTTCCACTTCAAAGTCCCAACACGTCGCACATTC
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      CTGGACCCCCAGAGCTGTGGGGACCTGCTGCAGGCTGTGCATCTGTTCGCCAAGGAACTG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      GATGCGAAAAGCGTCACGCTGGAGAGGAGCCTTGGAGGAGGGCGGTTTGGGGAGCTGTGC
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      TGTGGCTGCTTGCAGCTCCCCGGTCGCCAGGAGCTGCTCGTAGCCGTGCATATGCTGAGG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      GACAGCGCCTCCGACTCACAGAGGCTCGGCTTCCTGGCCGAGGCCCTCACGCTGGGCCAG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      TTTGACCATAGCCACATCGTGCGGCTGGAGGGCGTTGTTACCCGAGGAAGCACCTTGATG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      ATTGTCACCGAGTACATGAGCCATGGGGCCCTGGACGGCTTCCTCAGGCGGCACGAGGGG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      CAGCTGGTGGCTGGGCAACTGATGGGGTTGCTGCCTGGGCTGGCATCAGCCATGAAGTAT
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      CTGTCAGAGATGGGCTACGTTCACCGGGGCCTGGCAGCTCGCCATGTGCTGGTCAGCAGC
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      GACCTTGTCTGCAAGATCTCTGGCTTCGGGCGGGGCCCCCGGGACCGATCAGAGGCTGTC
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      TACACCACTATGAGTGGCCGGAGCCCAGCGCTATGGGCCGCTCCCGAGACACTTCAGTTT
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      GGCCACTTCAGCTCTGCCAGTGACGTGTGGAGCTTCGGCATCATCATGTGGGAGGTGATG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      GCCTTTGGGGAGCGGCCTTACTGGGACATGTCTGGCCAAGACGTGATCAAGGCTGTGGAG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      GATGGCTTCCGGCTGCCACCCCCCAGGAACTGTCCTAACCTTCTGCACCGACTAATGCTC
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      GACTGCTGGCAGAAGGACCCAGGTGAGCGGCCCAGGTTCTCCCAGATCCACAGCATCCTG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      AGCAAGATGGTGCAGGACCCAGAGCCCCCCAAGTGTGCCCTGACTACCTGTCCCAGGCCT
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      CCCACCCCACTAGCGGACCGTGCCTTCTCCACCTTCCCCTCCTTTGGCTCTGTGGGCGCG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      TGGCTGGAGGCCCTGGACCTGTGCCGCTACAAGGACAGCTTCGCGGCTGCTGGCTATGGG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      AGCCTGGAGGCCGTGGCCGAGATGACTGCCCAGGACCTGGTGAGCCTAGGCATCTCTTTG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ------------------------------------------------------------
NM_001099439.1      GCTGAACATCGAGAGGCCCTCCTCAGCGGGATCAGCGCCCTGCAGGCACGAGTGCTCCAG
NM_173641.2         ------------------------------------------------------------
                                                                                

HsCD00021533        ---------------------------
NM_001099439.1      CTGCAGGGCCAGGGGGTGCAGGTGTGA
NM_173641.2         ---------------------------
                                               

Protein

SMART domain : EPH_lbd : Ephrin receptor ligand binding domain
UniProt : Q8N289
UniProt : Q4G0R4
UniProt : Q5JZY3
HPRD : 08163

Clone

Cloning Information : 204043
HIP Master Clone ID : 178465
Original Clone ID : FLH204043.01F

Organism


TAX_ID : 9606
Species Specific ID: 284656

Genome

Chromosome : 1
Map Location : 1p34.3
Ensembl : ENSG00000183317

Reagents

Labome : EPHA10-antibody