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SPPL2A (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  SPPL2A
Gene Name:  signal peptide peptidase like 2A
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH159748.01F
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 24872421
Title: Regulated intramembrane proteolysis of the frontotemporal lobar degeneration (FTLD) risk factor,TMEM106B, bySignal Peptide Peptidase-like 2a (SPPL2a).
Authors: HIP
HsCD00021697

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1563nts         Open reading frame : 1 to 1563
Reference Sequence Alignment

ATGGGGCCGCAGCGGCGGCTGTCCCCTGCCGGGGCCGCCCTACTCTGGGGCTTCCTGCTC
CAGCTGACAGCCGCTCAGGAAGCAATCTTGCATGCGTCTGGAAATGGCACAACCAAGGAC
TACTGCATGCTTTATAACCCTTATTGGACAGCTCTTCCAAGTACCCTAGAAAATGCAACT
TCCATTAGTTTGATGAATCTGACTTCCACACCACTATGCAACCTTTCTGATATTCCTCCT
GTTGGCATAAAGAGCAAAGCAGTTGTGGTTCCATGGGGAAGCTGCCATTTTCTTGAAAAA
GCCAGAATTGCACAGAAAGGAGGTGCTGAAGCAATGTTAGTTGTCAATAACAGTGTCCTA
TTTCCTCCCTCAGGTAACAGATCTGAATTTCCTGATGTGAAAATACTGATTGCATTTATA
AGCTACAAAGACTTTAGAGATATGAACCAGACTCTAGGAGATAACATTACTGTGAAAATG
TATTCTCCATCGTGGCCTAACTTTGATTATACTATGGTGGTTATTTTTGTAATTGCGGTG
TTCACTGTGGCATTAGGTGGATACTGGAGTGGACTAGTTGAATTGGAAAACTTGAAAGCA
GTGACAACTGAAGATAGAGAAATGAGGAAAAAGAAGGAAGAATATTTAACTTTTAGTCCT
CTTACAGTTGTAATATTTGTGGTCATCTGCTGTGTTATGATGGTCTTACTTTATTTCTTC
TACAAATGGTTGGTTTATGTTATGATAGCAATTTTCTGCATAGCATCAGCAATGAGTCTG
TACAACTGTCTTGCTGCACTAATTCATAAGATACCATATGGACAATGCACGATTGCATGT
CGTGGCAAAAACATGGAAGTGAGACTTATTTTTCTCTCTGGACTGTGCATAGCAGTAGCT
GTTGTTTGGGCTGTGTTTCGAAATGAAGACAGGTGGGCTTGGATTTTACAGGATATCTTG
GGGATTGCTTTCTGTCTGAATTTAATTAAAACACTGAAGTTGCCCAACTTCAAGTCATGT
GTGATACTTCTAGGCCTTCTCCTCCTCTATGATGTATTTTTTGTTTTCATAACACCATTC
ATCACAAAGAATGGTGAGAGTATCATGGTTGAACTCGCAGCTGGACCTTTTGGAAATAAT
GAAAAGTTGCCAGTAGTCATCAGAGTACCAAAACTGATCTATTTCTCAGTAATGAGTGTG
TGCCTCATGCCTGTTTCAATATTGGGTTTTGGAGACATTATTGTACCAGGCCTGTTGATT
GCATACTGTAGAAGATTTGATGTTCAGACTGGTTCTTCTTACATATACTATGTTTCGTCT
ACAGTTGCCTATGCTATTGGCATGATACTTACATTTGTTGTTCTGGTGCTGATGAAAAAG
GGGCAACCTGCTCTCCTCTATTTAGTACCTTGCACACTTATTACTGCCTCAGTTGTTGCC
TGGAGACGTAAGGAAATGAAAAAGTTCTGGAAAGGTAACAGCTATCAGATGATGGACCAT
TTGGATTGTGCAACAAATGAAGAAAACCCTGTGATATCTGGTGAACCGATTGTCCAGCAA
TTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1706
Start on reference sequence 144
Mutations:
None
Discrepancy:
a1547c,Q516P
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SPPL2A
Symbol Nomenclature : SPPL2A
SYNONYM : IMP3; PSL2
Designation : IMP-3|SPP-like 2A|intramembrane cleaving protease|intramembrane protease 3|presenilin-like protein 2|signal peptide peptidase-like 2A
Full Nomenclature : signal peptide peptidase like 2A
GENEID : 84888
Locus Tag : PSEC0147
HGNC : 30227
MIM : 608238
Vega : OTTHUMG00000131647
Target GenBank: BC025740

 

Reference Sequence Alignment

  

HsCD00021697        ATGGGGCCGCAGCGGCGGCTGTCCCCTGCCGGGGCCGCCCTACTCTGGGGCTTCCTGCTC
XM_005254722.1      ATGGGGCCGCAGCGGCGGCTGTCCCCTGCCGGGGCCGCCCTACTCTGGGGCTTCCTGCTC
NM_032802.3         ATGGGGCCGCAGCGGCGGCTGTCCCCTGCCGGGGCCGCCCTACTCTGGGGCTTCCTGCTC
                    ************************************************************

HsCD00021697        CAGCTGACAGCCGCTCAGGAAGCAATCTTGCATGCGTCTGGAAATGGCACAACCAAGGAC
XM_005254722.1      CAGCTGACAGCCGCTCAGGAAGCAATCTTGCATGCGTCTGGAAATGGCACAACCAAGGAC
NM_032802.3         CAGCTGACAGCCGCTCAGGAAGCAATCTTGCATGCGTCTGGAAATGGCACAACCAAGGAC
                    ************************************************************

HsCD00021697        TACTGCATGCTTTATAACCCTTATTGGACAGCTCTTCCAAGTACCCTAGAAAATGCAACT
XM_005254722.1      TACTGCATGCTTTATAACCCTTATTGGACAGCTCTTCCAAGTACCCTAGAAAATGCAACT
NM_032802.3         TACTGCATGCTTTATAACCCTTATTGGACAGCTCTTCCAAGTACCCTAGAAAATGCAACT
                    ************************************************************

HsCD00021697        TCCATTAGTTTGATGAATCTGACTTCCACACCACTATGCAACCTTTCTGATATTCCTCCT
XM_005254722.1      TCCATTAGTTTGATGAATCTGACTTCCACACCACTATGCAACCTTTCTGATATTCCTCCT
NM_032802.3         TCCATTAGTTTGATGAATCTGACTTCCACACCACTATGCAACCTTTCTGATATTCCTCCT
                    ************************************************************

HsCD00021697        GTTGGCATAAAGAGCAAAGCAGTTGTGGTTCCATGGGGAAGCTGCCATTTTCTTGAAAAA
XM_005254722.1      GTTGGCATAAAGAGCAAAGCAGTTGTGGTTCCATGGGGAAGCTGCCATTTTCTTGAAAAA
NM_032802.3         GTTGGCATAAAGAGCAAAGCAGTTGTGGTTCCATGGGGAAGCTGCCATTTTCTTGAAAAA
                    ************************************************************

HsCD00021697        GCCAGAATTGCACAGAAAGGAGGTGCTGAAGCAATGTTAGTTGTCAATAACAGTGTCCTA
XM_005254722.1      GCCAGAATTGCACAGAAAGGAGGTGCTGAAGCAATGTTAGTTGTCAATAACAGTGTCCTA
NM_032802.3         GCCAGAATTGCACAGAAAGGAGGTGCTGAAGCAATGTTAGTTGTCAATAACAGTGTCCTA
                    ************************************************************

HsCD00021697        TTTCCTCCCTCAGGTAACAGATCTGAATTTCCTGATGTGAAAATACTGATTGCATTTATA
XM_005254722.1      TTTCCTCCCTCAGGTAACAGATCTGAATTTCCTGATGTGAAAATACTGATTGCATTTATA
NM_032802.3         TTTCCTCCCTCAGGTAACAGATCTGAATTTCCTGATGTGAAAATACTGATTGCATTTATA
                    ************************************************************

HsCD00021697        AGCTACAAAGACTTTAGAGATATGAACCAGACTCTAGGAGATAACATTACTGTGAAAATG
XM_005254722.1      AGCTACAAAGACTTTAGAGATATGAACCAGACTCTAGGAGATAACATTACTGTGAAAATG
NM_032802.3         AGCTACAAAGACTTTAGAGATATGAACCAGACTCTAGGAGATAACATTACTGTGAAAATG
                    ************************************************************

HsCD00021697        TATTCTCCATCGTGGCCTAACTTTGATTATACTATGGTGGTTATTTTTGTAATTGCGGTG
XM_005254722.1      TATTCTCCATCGTGGCCTAACTTTGATTATACTATGGTGGTTATTTTTGTAATTGCGGTG
NM_032802.3         TATTCTCCATCGTGGCCTAACTTTGATTATACTATGGTGGTTATTTTTGTAATTGCGGTG
                    ************************************************************

HsCD00021697        TTCACTGTGGCATTAGGTGGATACTGGAGTGGACTAGTTGAATTGGAAAACTTGAAAGCA
XM_005254722.1      TTCACTGTGGCATTAGGTGGATACTGGAGTGGACTAGTTGAATTGGAAAACTTGAAAGCA
NM_032802.3         TTCACTGTGGCATTAGGTGGATACTGGAGTGGACTAGTTGAATTGGAAAACTTGAAAGCA
                    ************************************************************

HsCD00021697        GTGACAACTGAAGATAGAGAAATGAGGAAAAAGAAGGAAGAATATTTAACTTTTAGTCCT
XM_005254722.1      GTGACAACTGAAGATAGAGAAATGAGGAAAAAGAAGGAAGAATATTTAACTTTTAGTCCT
NM_032802.3         GTGACAACTGAAGATAGAGAAATGAGGAAAAAGAAGGAAGAATATTTAACTTTTAGTCCT
                    ************************************************************

HsCD00021697        CTTACAGTTGTAATATTTGTGGTCATCTGCTGTGTTATGATGGTCTTACTTTATTTCTTC
XM_005254722.1      CTTACAGTTGTAATATTTGTGGTCATCTGCTGTGTTATGATGGTCTTACTTTATTTCTTC
NM_032802.3         CTTACAGTTGTAATATTTGTGGTCATCTGCTGTGTTATGATGGTCTTACTTTATTTCTTC
                    ************************************************************

HsCD00021697        TACAAATGGTTGGTTTATGTTATGATAGCAATTTTCTGCATAGCATCAGCAATGAGTCTG
XM_005254722.1      TACAAATGGTTGGTTTATGTTATGATAGCAATTTTCTGCATAGCATCAGCAATGAGTCTG
NM_032802.3         TACAAATGGTTGGTTTATGTTATGATAGCAATTTTCTGCATAGCATCAGCAATGAGTCTG
                    ************************************************************

HsCD00021697        TACAACTGTCTTGCTGCACTAATTCATAAGATACCATATGGACAATGCACGATTGCATGT
XM_005254722.1      TACAACTGTCTTGCTGCACTAATTCATAAGATACCATATGGACAATGCACGATTGCATGT
NM_032802.3         TACAACTGTCTTGCTGCACTAATTCATAAGATACCATATGGACAATGCACGATTGCATGT
                    ************************************************************

HsCD00021697        CGTGGCAAAAACATGGAAGTGAGACTTATTTTTCTCTCTGGACTGTGCATAGCAGTAGCT
XM_005254722.1      CGTGGCAAAAACATGGAAGTGAGACTTATTTTTCTCTCTGGACTGTGCATAGCAGTAGCT
NM_032802.3         CGTGGCAAAAACATGGAAGTGAGACTTATTTTTCTCTCTGGACTGTGCATAGCAGTAGCT
                    ************************************************************

HsCD00021697        GTTGTTTGGGCTGTGTTTCGAAATGAAGACAGGTGGGCTTGGATTTTACAGGATATCTTG
XM_005254722.1      GTTGTTTGGGCTGTGTTTCGAAATGAAGACAGGTGGGCTTGGATTTTACAGGATATCTTG
NM_032802.3         GTTGTTTGGGCTGTGTTTCGAAATGAAGACAGGTGGGCTTGGATTTTACAGGATATCTTG
                    ************************************************************

HsCD00021697        GGGATTGCTTTCTGTCTGAATTTAATTAAAACACTGAAGTTGCCCAACTTCAAGTCATGT
XM_005254722.1      GGGATTGCTTTCTGTCTGAATTTAATTAAAACACTGAAGTTGCCCAACTTCAAGTCATGT
NM_032802.3         GGGATTGCTTTCTGTCTGAATTTAATTAAAACACTGAAGTTGCCCAACTTCAAGTCATGT
                    ************************************************************

HsCD00021697        GTGATACTTCTAGGCCTTCTCCTCCTCTATGATGTATTTTTTGTTTTCATAACACCATTC
XM_005254722.1      GTGATACTTCTAGGCCTTCTCCTCCTCTATGATGTATTTTTTGTTTTCATAACACCATTC
NM_032802.3         GTGATACTTCTAGGCCTTCTCCTCCTCTATGATGTATTTTTTGTTTTCATAACACCATTC
                    ************************************************************

HsCD00021697        ATCACAAAGAATGGTGAGAGTATCATGGTTGAACTCGCAGCTGGACCTTTTGGAAATAAT
XM_005254722.1      ATCACAAAGAATGGTGAGAGTATCATGGTTGAACTCGCAGCTGGACCTTTTGGAAATAAT
NM_032802.3         ATCACAAAGAATGGTGAGAGTATCATGGTTGAACTCGCAGCTGGACCTTTTGGAAATAAT
                    ************************************************************

HsCD00021697        GAAAAGTTG---------------------------------------------------
XM_005254722.1      GAAAAG---AATGATGGAAACTTGGTGGAAGCCACTGGTCAACCCTCAGCTCCCCATGAG
NM_032802.3         GAAAAG---TTG------------------------------------------------
                    ******                                                      

HsCD00021697        ------CCAGTAGTCATCAGAGTACCAAAACTGATCTATTTCTCAGTAATGAGTGTGTGC
XM_005254722.1      AAATTGCCAGTAGTCATCAGAGTACCAAAACTGATCTATTTCTCAGTAATGAGTGTGTGC
NM_032802.3         ------CCAGTAGTCATCAGAGTACCAAAACTGATCTATTTCTCAGTAATGAGTGTGTGC
                          ******************************************************

HsCD00021697        CTCATGCCTGTTTCAATATTGGGTTTTGGAGACATTATTGTACCAGGCCTGTTGATTGCA
XM_005254722.1      CTCATGCCTGTTTCAATATTGGGTTTTGGAGACATTATTGTACCAGGCCTGTTGATTGCA
NM_032802.3         CTCATGCCTGTTTCAATATTGGGTTTTGGAGACATTATTGTACCAGGCCTGTTGATTGCA
                    ************************************************************

HsCD00021697        TACTGTAGAAGATTTGATGTTCAGACTGGTTCTTCTTACATATACTATGTTTCGTCTACA
XM_005254722.1      TACTGTAGAAGATTTGATGTTCAGACTGGTTCTTCTTACATATACTATGTTTCGTCTACA
NM_032802.3         TACTGTAGAAGATTTGATGTTCAGACTGGTTCTTCTTACATATACTATGTTTCGTCTACA
                    ************************************************************

HsCD00021697        GTTGCCTATGCTATTGGCATGATACTTACATTTGTTGTTCTGGTGCTGATGAAAAAGGGG
XM_005254722.1      GTTGCCTATGCTATTGGCATGATACTTACATTTGTTGTTCTGGTGCTGATGAAAAAGGGG
NM_032802.3         GTTGCCTATGCTATTGGCATGATACTTACATTTGTTGTTCTGGTGCTGATGAAAAAGGGG
                    ************************************************************

HsCD00021697        CAACCTGCTCTCCTCTATTTAGTACCTTGCACACTTATTACTGCCTCAGTTGTTGCCTGG
XM_005254722.1      CAACCTGCTCTCCTCTATTTAGTACCTTGCACACTTATTACTGCCTCAGTTGTTGCCTGG
NM_032802.3         CAACCTGCTCTCCTCTATTTAGTACCTTGCACACTTATTACTGCCTCAGTTGTTGCCTGG
                    ************************************************************

HsCD00021697        AGACGTAAGGAAATGAAAAAGTTCTGGAAAGGTAACAGCTATCAGATGATGGACCATTTG
XM_005254722.1      AGACGTAAGGAAATGAAAAAGTTCTGGAAAGGTAACAGCTATCAGATGATGGACCATTTG
NM_032802.3         AGACGTAAGGAAATGAAAAAGTTCTGGAAAGGTAACAGCTATCAGATGATGGACCATTTG
                    ************************************************************

HsCD00021697        GATTGTGCAACAAATGAAGAAAACCCTGTGATATCTGGTGAACCGATTGTCCAGCAATTG
XM_005254722.1      GATTGTGCAACAAATGAAGAAAACCCTGTGATATCTGGTGAACAGATTGTCCAGCAATAA
NM_032802.3         GATTGTGCAACAAATGAAGAAAACCCTGTGATATCTGGTGAACAGATTGTCCAGCAATAA
                    *******************************************.**************:.

Protein

SMART domain : PSN : Presenilin, signal peptide peptidase, family
UniProt : Q8TCT8
HPRD : 09742

Clone

Cloning Information : 159748
HIP Master Clone ID : 135283
Original Clone ID : FLH159748.01F

Organism


TAX_ID : 9606
Species Specific ID: 84888

Genome

Chromosome : 15
Map Location : 15q21.2
Ensembl : ENSG00000138600

Reagents

Labome : SPPL2A-antibody