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TOMM20 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TOMM20
Gene Name:  translocase of outer mitochondrial membrane 20 homolog (yeast)
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH159913.01F
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00021724

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 438nts         Open reading frame : 1 to 438
Reference Sequence Alignment

ATGGTGGGTCGGAACAGCGCCATCGCCGCCGGTGTATGCGGGGCCCTTTTCATTGGGTAC
TGCATCTACTTCGACCGCAAAAGACGAAGTGACCCCAACTTCAAGAACAGGCTTCGAGAA
CGAAGAAAGAAACAGAAGCTTGCCAAGGAGAGAGCTGGGCTTTCCAAGTTACCTGACCTT
AAAGATGCTGAAGCTGTTCAGAAGTTCTTCCTTGAAGAAATACAGCTTGGTGAAGAGTTA
CTAGCTCAAGGTGAATATGAGAAGGGCGTAGACCATCTGACAAATGCAATTGCTGTGTGT
GGACAGCCACAGCAGTTACTGCAGGTCTTACAGCAAACTCTTCCACCACCAGTGTTCCAG
ATGCTTCTGACTAAGCTCCCAACAATTAGTCAGAGAATTGTAAGTGCTCAGAGCTTGGCT
GAAGATGATGTGGAATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 556
Start on reference sequence 119
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TOMM20
Symbol Nomenclature : TOMM20
SYNONYM : MAS20; MOM19; TOM20
Designation : mitochondrial 20 kDa outer membrane protein|mitochondrial import receptor subunit TOM20 homolog|outer mitochondrial membrane receptor Tom20|translocase of outer mitochondrial membrane 20 homolog type II
Full Nomenclature : translocase of outer mitochondrial membrane 20 homolog (yeast)
GENEID : 9804
Locus Tag : RP4-597N16.2
HGNC : 20947
MIM : 601848
Vega : OTTHUMG00000039619
Target GenBank: BC000882

 

Reference Sequence Alignment

  

HsCD00021724      ATGGTGGGTCGGAACAGCGCCATCGCCGCCGGTGTATGCGGGGCCCTTTTCATTGGGTAC
NM_014765.2       ATGGTGGGTCGGAACAGCGCCATCGCCGCCGGTGTATGCGGGGCCCTTTTCATTGGGTAC
                  ************************************************************

HsCD00021724      TGCATCTACTTCGACCGCAAAAGACGAAGTGACCCCAACTTCAAGAACAGGCTTCGAGAA
NM_014765.2       TGCATCTACTTCGACCGCAAAAGACGAAGTGACCCCAACTTCAAGAACAGGCTTCGAGAA
                  ************************************************************

HsCD00021724      CGAAGAAAGAAACAGAAGCTTGCCAAGGAGAGAGCTGGGCTTTCCAAGTTACCTGACCTT
NM_014765.2       CGAAGAAAGAAACAGAAGCTTGCCAAGGAGAGAGCTGGGCTTTCCAAGTTACCTGACCTT
                  ************************************************************

HsCD00021724      AAAGATGCTGAAGCTGTTCAGAAGTTCTTCCTTGAAGAAATACAGCTTGGTGAAGAGTTA
NM_014765.2       AAAGATGCTGAAGCTGTTCAGAAGTTCTTCCTTGAAGAAATACAGCTTGGTGAAGAGTTA
                  ************************************************************

HsCD00021724      CTAGCTCAAGGTGAATATGAGAAGGGCGTAGACCATCTGACAAATGCAATTGCTGTGTGT
NM_014765.2       CTAGCTCAAGGTGAATATGAGAAGGGCGTAGACCATCTGACAAATGCAATTGCTGTGTGT
                  ************************************************************

HsCD00021724      GGACAGCCACAGCAGTTACTGCAGGTCTTACAGCAAACTCTTCCACCACCAGTGTTCCAG
NM_014765.2       GGACAGCCACAGCAGTTACTGCAGGTCTTACAGCAAACTCTTCCACCACCAGTGTTCCAG
                  ************************************************************

HsCD00021724      ATGCTTCTGACTAAGCTCCCAACAATTAGTCAGAGAATTGTAAGTGCTCAGAGCTTGGCT
NM_014765.2       ATGCTTCTGACTAAGCTCCCAACAATTAGTCAGAGAATTGTAAGTGCTCAGAGCTTGGCT
                  ************************************************************

HsCD00021724      GAAGATGATGTGGAATTG
NM_014765.2       GAAGATGATGTGGAATGA
                  **************** .

Pathways

Reactome : Metabolism of proteins
Reactome : Mitochondrial protein import

Protein

UniProt : Q15388
HPRD : 03507

Clone

Cloning Information : 159913
HIP Master Clone ID : 135448
Original Clone ID : FLH159913.01F

Organism


TAX_ID : 9606
Species Specific ID: 9804

Genome

Chromosome : 1
Map Location : 1q42
Ensembl : ENSG00000173726

Reagents

Labome : Tom20-antibody