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CAMK2A (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CAMK2A
Gene Name:  calcium/calmodulin-dependent protein kinase II alpha
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH000243.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors: HIP
HsCD00021854

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1437nts         Open reading frame : 1 to 1437
Reference Sequence Alignment

ATGGCTACCATCACCTGCACCCGCTTCACGGAAGAGTACCAGCTCTTCGAGGAATTGGGC
AAGGGAGCCTTCTCGGTGGTGCGAAGGTGTGTGAAGGTGCTGGCTGGCCAGGAGTATGCT
GCCAAGATCATCAACACAAAGAAGCTGTCAGCCAGAGACCATCAGAAGCTGGAGCGTGAA
GCCCGCATCTGCCGCCTGCTGAAGCACCCCAACATCGTCCGACTACATGACAGCATCTCA
GAGGAGGGACACCACTACCTGATCTTCGACCTGGTCACTGGTGGGGAACTGTTTGAAGAT
ATCGTGGCCCGGGAGTATTACAGTGAGGCGGATGCCAGTCACTGTATCCAGCAGATCCTG
GAGGCTGTGCTGCACTGCCACCAGATGGGGGTGGTGCACCGGGACCTGAAGCCTGAGAAT
CTGTTGCTGGCCTCCAAGCTCAAGGGTGCCGCAGTGAAGCTGGCAGACTTTGGCCTGGCC
ATAGAGGTGGAGGGGGAGCAGCAGGCATGGTTTGGGTTTGCAGGGACTCCTGGATATCTC
TCCCCAGAAGTGCTGCGGAAGGACCCGTACGGGAAGCCTGTGGACCTGTGGGCTTGTGGG
GTCATCCTGTACATCCTGCTGGTTGGGTACCCCCCGTTCTGGGATGAGGACCAGCACCGC
CTGTACCTGCAGATCAAAGCCGGCGCCTATGATTTCCCATCGCCGGAATGGGACACTGTC
ACCCCGGAAGCCAAGGATCTGATCAATAAGATGCTGACCATTAACCCATCCAAACGCATC
ACAGCTGCCGAAGCCCTTAAGCACCCCTGGATCTCGCACCGCTCCACCGTGGCATCCTGC
ATGCACAGACAGGAGACCGTGGACTGCCTGAAGAAGTTCAATGCCAGGAGGAAACTGAAG
GGAGCCATTCTCACCACGATGCTGGCCACCAGGAACTTCTCCGGAGGGAAGAGTGGGGGA
AACAAGAAGAGCGATGGTGTGAAGGAATCCTCAGAGAGCACCAACACCACCATCGAGGAT
GAAGACACCAAAGTGCGGAAACAGGAAATTATAAAAGTGACAGAGCAGCTGATTGAAGCC
ATAAGCAATGGAGATTTTGAGTCCTACACGAAGATGTGCGACCCTGGCATGACAGCCTTC
GAACCTGAGGCCCTGGGGAACCTGGTTGAGGGCCTGGACTTCCATCGATTCTATTTTGAA
AACCTGTGGTCCCGGAACAGCAAGCCCGTGCACACCACCATCCTGAATCCCCACATCCAC
CTGATGGGCGACGAGTCAGCCTGCATCGCCTACATCCGCATCACGCAGTACCTGGACGCT
GGCGGCATCCCACGCACCGCCCAGTCGGAGGAGACCCGTGTCTGGCACCGCCGGGACGGC
AAATGGCAGATCGTCCACTTCCACAGATCTGGGGCGCCCTCCGTCCTGCCCCATTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1585
Start on reference sequence 149
Mutations:
None
Discrepancy:
c6t; a668t,Q223L;
 t1377c; c1434t
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAMK2A
Symbol Nomenclature : CAMK2A
SYNONYM : CAMKA
Designation : CaM kinase II alpha subunit|CaM-kinase II alpha chain|CaMK-II alpha subunit|CaMKIINalpha|caM kinase II subunit alpha|caMK-II subunit alpha|calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha|calcium/calmodulin-dependent protein kinase II alpha-B subunit|calcium/calmodulin-dependent protein kinase type II alpha chain|calcium/calmodulin-dependent protein kinase type II subunit alpha
Full Nomenclature : calcium/calmodulin-dependent protein kinase II alpha
GENEID : 815
GI : 33304056
GenBank Accession : AY335709
HGNC : 1460
MIM : 114078
Vega : OTTHUMG00000134281
Target GenBank: NM_171825

 

Reference Sequence Alignment

  

HsCD00021854      ATGGCTACCATCACCTGCACCCGCTTCACGGAAGAGTACCAGCTCTTCGAGGAATTGGGC
NM_015981.3       ATGGCCACCATCACCTGCACCCGCTTCACGGAAGAGTACCAGCTCTTCGAGGAATTGGGC
NM_171825.2       ATGGCCACCATCACCTGCACCCGCTTCACGGAAGAGTACCAGCTCTTCGAGGAATTGGGC
                  ***** ******************************************************

HsCD00021854      AAGGGAGCCTTCTCGGTGGTGCGAAGGTGTGTGAAGGTGCTGGCTGGCCAGGAGTATGCT
NM_015981.3       AAGGGAGCCTTCTCGGTGGTGCGAAGGTGTGTGAAGGTGCTGGCTGGCCAGGAGTATGCT
NM_171825.2       AAGGGAGCCTTCTCGGTGGTGCGAAGGTGTGTGAAGGTGCTGGCTGGCCAGGAGTATGCT
                  ************************************************************

HsCD00021854      GCCAAGATCATCAACACAAAGAAGCTGTCAGCCAGAGACCATCAGAAGCTGGAGCGTGAA
NM_015981.3       GCCAAGATCATCAACACAAAGAAGCTGTCAGCCAGAGACCATCAGAAGCTGGAGCGTGAA
NM_171825.2       GCCAAGATCATCAACACAAAGAAGCTGTCAGCCAGAGACCATCAGAAGCTGGAGCGTGAA
                  ************************************************************

HsCD00021854      GCCCGCATCTGCCGCCTGCTGAAGCACCCCAACATCGTCCGACTACATGACAGCATCTCA
NM_015981.3       GCCCGCATCTGCCGCCTGCTGAAGCACCCCAACATCGTCCGACTACATGACAGCATCTCA
NM_171825.2       GCCCGCATCTGCCGCCTGCTGAAGCACCCCAACATCGTCCGACTACATGACAGCATCTCA
                  ************************************************************

HsCD00021854      GAGGAGGGACACCACTACCTGATCTTCGACCTGGTCACTGGTGGGGAACTGTTTGAAGAT
NM_015981.3       GAGGAGGGACACCACTACCTGATCTTCGACCTGGTCACTGGTGGGGAACTGTTTGAAGAT
NM_171825.2       GAGGAGGGACACCACTACCTGATCTTCGACCTGGTCACTGGTGGGGAACTGTTTGAAGAT
                  ************************************************************

HsCD00021854      ATCGTGGCCCGGGAGTATTACAGTGAGGCGGATGCCAGTCACTGTATCCAGCAGATCCTG
NM_015981.3       ATCGTGGCCCGGGAGTATTACAGTGAGGCGGATGCCAGTCACTGTATCCAGCAGATCCTG
NM_171825.2       ATCGTGGCCCGGGAGTATTACAGTGAGGCGGATGCCAGTCACTGTATCCAGCAGATCCTG
                  ************************************************************

HsCD00021854      GAGGCTGTGCTGCACTGCCACCAGATGGGGGTGGTGCACCGGGACCTGAAGCCTGAGAAT
NM_015981.3       GAGGCTGTGCTGCACTGCCACCAGATGGGGGTGGTGCACCGGGACCTGAAGCCTGAGAAT
NM_171825.2       GAGGCTGTGCTGCACTGCCACCAGATGGGGGTGGTGCACCGGGACCTGAAGCCTGAGAAT
                  ************************************************************

HsCD00021854      CTGTTGCTGGCCTCCAAGCTCAAGGGTGCCGCAGTGAAGCTGGCAGACTTTGGCCTGGCC
NM_015981.3       CTGTTGCTGGCCTCCAAGCTCAAGGGTGCCGCAGTGAAGCTGGCAGACTTTGGCCTGGCC
NM_171825.2       CTGTTGCTGGCCTCCAAGCTCAAGGGTGCCGCAGTGAAGCTGGCAGACTTTGGCCTGGCC
                  ************************************************************

HsCD00021854      ATAGAGGTGGAGGGGGAGCAGCAGGCATGGTTTGGGTTTGCAGGGACTCCTGGATATCTC
NM_015981.3       ATAGAGGTGGAGGGGGAGCAGCAGGCATGGTTTGGGTTTGCAGGGACTCCTGGATATCTC
NM_171825.2       ATAGAGGTGGAGGGGGAGCAGCAGGCATGGTTTGGGTTTGCAGGGACTCCTGGATATCTC
                  ************************************************************

HsCD00021854      TCCCCAGAAGTGCTGCGGAAGGACCCGTACGGGAAGCCTGTGGACCTGTGGGCTTGTGGG
NM_015981.3       TCCCCAGAAGTGCTGCGGAAGGACCCGTACGGGAAGCCTGTGGACCTGTGGGCTTGTGGG
NM_171825.2       TCCCCAGAAGTGCTGCGGAAGGACCCGTACGGGAAGCCTGTGGACCTGTGGGCTTGTGGG
                  ************************************************************

HsCD00021854      GTCATCCTGTACATCCTGCTGGTTGGGTACCCCCCGTTCTGGGATGAGGACCAGCACCGC
NM_015981.3       GTCATCCTGTACATCCTGCTGGTTGGGTACCCCCCGTTCTGGGATGAGGACCAGCACCGC
NM_171825.2       GTCATCCTGTACATCCTGCTGGTTGGGTACCCCCCGTTCTGGGATGAGGACCAGCACCGC
                  ************************************************************

HsCD00021854      CTGTACCTGCAGATCAAAGCCGGCGCCTATGATTTCCCATCGCCGGAATGGGACACTGTC
NM_015981.3       CTGTACCAGCAGATCAAAGCCGGCGCCTATGATTTCCCATCGCCGGAATGGGACACTGTC
NM_171825.2       CTGTACCAGCAGATCAAAGCCGGCGCCTATGATTTCCCATCGCCGGAATGGGACACTGTC
                  *******:****************************************************

HsCD00021854      ACCCCGGAAGCCAAGGATCTGATCAATAAGATGCTGACCATTAACCCATCCAAACGCATC
NM_015981.3       ACCCCGGAAGCCAAGGATCTGATCAATAAGATGCTGACCATTAACCCATCCAAACGCATC
NM_171825.2       ACCCCGGAAGCCAAGGATCTGATCAATAAGATGCTGACCATTAACCCATCCAAACGCATC
                  ************************************************************

HsCD00021854      ACAGCTGCCGAAGCCCTTAAGCACCCCTGGATCTCGCACCGCTCCACCGTGGCATCCTGC
NM_015981.3       ACAGCTGCCGAAGCCCTTAAGCACCCCTGGATCTCGCACCGCTCCACCGTGGCATCCTGC
NM_171825.2       ACAGCTGCCGAAGCCCTTAAGCACCCCTGGATCTCGCACCGCTCCACCGTGGCATCCTGC
                  ************************************************************

HsCD00021854      ATGCACAGACAGGAGACCGTGGACTGCCTGAAGAAGTTCAATGCCAGGAGGAAACTGAAG
NM_015981.3       ATGCACAGACAGGAGACCGTGGACTGCCTGAAGAAGTTCAATGCCAGGAGGAAACTGAAG
NM_171825.2       ATGCACAGACAGGAGACCGTGGACTGCCTGAAGAAGTTCAATGCCAGGAGGAAACTGAAG
                  ************************************************************

HsCD00021854      GGAGCCATTCTCACCACGATGCTGGCCACCAGGAACTTCTCCGGAGGGAAGAGTGGGGGA
NM_015981.3       GGAGCCATTCTCACCACGATGCTGGCCACCAGGAACTTCTCCGGAGGGAAGAGTGGGGGA
NM_171825.2       GGAGCCATTCTCACCACGATGCTGGCCACCAGGAACTTCTCCGGAGGGAAGAGTGGGGGA
                  ************************************************************

HsCD00021854      AACAAGAAGAGCGATG---------------------------------GTGTGAAGGAA
NM_015981.3       AACAAGAAGAGCGATGGTGTGAAGAAAAGAAAGTCCAGTTCCAGCGTTCAGTTAATGGAA
NM_171825.2       AACAAGAAGAGCGATG---------------------------------GTGTGAAGGAA
                  ****************                                 .  *.*:****

HsCD00021854      TCCTCAGAGAGCACCAACACCACCATCGAGGATGAAGACACCAAAGTGCGGAAACAGGAA
NM_015981.3       TCCTCAGAGAGCACCAACACCACCATCGAGGATGAAGACACCAAAGTGCGGAAACAGGAA
NM_171825.2       TCCTCAGAGAGCACCAACACCACCATCGAGGATGAAGACACCAAAGTGCGGAAACAGGAA
                  ************************************************************

HsCD00021854      ATTATAAAAGTGACAGAGCAGCTGATTGAAGCCATAAGCAATGGAGATTTTGAGTCCTAC
NM_015981.3       ATTATAAAAGTGACAGAGCAGCTGATTGAAGCCATAAGCAATGGAGATTTTGAGTCCTAC
NM_171825.2       ATTATAAAAGTGACAGAGCAGCTGATTGAAGCCATAAGCAATGGAGATTTTGAGTCCTAC
                  ************************************************************

HsCD00021854      ACGAAGATGTGCGACCCTGGCATGACAGCCTTCGAACCTGAGGCCCTGGGGAACCTGGTT
NM_015981.3       ACGAAGATGTGCGACCCTGGCATGACAGCCTTCGAACCTGAGGCCCTGGGGAACCTGGTT
NM_171825.2       ACGAAGATGTGCGACCCTGGCATGACAGCCTTCGAACCTGAGGCCCTGGGGAACCTGGTT
                  ************************************************************

HsCD00021854      GAGGGCCTGGACTTCCATCGATTCTATTTTGAAAACCTGTGGTCCCGGAACAGCAAGCCC
NM_015981.3       GAGGGCCTGGACTTCCATCGATTCTATTTTGAAAACCTGTGGTCCCGGAACAGCAAGCCC
NM_171825.2       GAGGGCCTGGACTTCCATCGATTCTATTTTGAAAACCTGTGGTCCCGGAACAGCAAGCCC
                  ************************************************************

HsCD00021854      GTGCACACCACCATCCTGAATCCCCACATCCACCTGATGGGCGACGAGTCAGCCTGCATC
NM_015981.3       GTGCACACCACCATCCTGAATCCCCACATCCACCTGATGGGCGACGAGTCAGCCTGCATC
NM_171825.2       GTGCACACCACCATCCTGAATCCCCACATCCACCTGATGGGCGACGAGTCAGCCTGCATC
                  ************************************************************

HsCD00021854      GCCTACATCCGCATCACGCAGTACCTGGACGCTGGCGGCATCCCACGCACCGCCCAGTCG
NM_015981.3       GCCTACATCCGCATCACGCAGTACCTGGACGCTGGCGGCATCCCACGCACCGCCCAGTCG
NM_171825.2       GCCTACATCCGCATCACGCAGTACCTGGACGCTGGCGGCATCCCACGCACCGCCCAGTCG
                  ************************************************************

HsCD00021854      GAGGAGACCCGTGTCTGGCACCGCCGGGACGGCAAATGGCAGATCGTCCACTTCCACAGA
NM_015981.3       GAGGAGACCCGTGTCTGGCACCGCCGGGATGGCAAATGGCAGATCGTCCACTTCCACAGA
NM_171825.2       GAGGAGACCCGTGTCTGGCACCGCCGGGATGGCAAATGGCAGATCGTCCACTTCCACAGA
                  ***************************** ******************************

HsCD00021854      TCTGGGGCGCCCTCCGTCCTGCCCCATTTG
NM_015981.3       TCTGGGGCGCCCTCCGTCCTGCCCCACTGA
NM_171825.2       TCTGGGGCGCCCTCCGTCCTGCCCCACTGA
                  ************************** * .

Pathways

NCI : FGF signaling pathway
NCI : IFN-gamma pathway
NCI : Netrin-mediated signaling events
NCI : Noncanonical Wnt signaling pathway
NCI : TGF-beta receptor signaling
NCI : Trk receptor signaling mediated by PI3K and PLC-gamma
Panther : Inflammation mediated by chemokine and cytokine signaling pathway
Panther : Ionotropic glutamate receptor pathway
Reactome : Activation of NMDA receptor upon glutamate binding and postsynaptic events
Reactome : CREB phosphorylation through the activation of CaMKII
Reactome : CREB phosphorylation through the activation of Ras
Reactome : Ca2+ pathway
Reactome : Cellular response to heat stress
Reactome : Cellular responses to stress
Reactome : Cytokine Signaling in Immune system
Reactome : Glutamate Binding, Activation of AMPA Receptors and Synaptic Plasticity
Reactome : HSF1-dependent transactivation
Reactome : Immune System
Reactome : Interferon Signaling
Reactome : Interferon gamma signaling
Reactome : Neuronal System
Reactome : Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome : Post NMDA receptor activation events
Reactome : Ras activation uopn Ca2+ infux through NMDA receptor
Reactome : Signal Transduction
Reactome : Signaling by Wnt
Reactome : Trafficking of AMPA receptors
Reactome : Transmission across Chemical Synapses
Reactome : Unblocking of NMDA receptor, glutamate binding and activation
Reactome : beta-catenin independent WNT signaling
WikiPathway : B Cell Receptor Signaling Pathway
WikiPathway : Calcium Regulation in the Cardiac Cell
WikiPathway : EGF/EGFR Signaling Pathway
WikiPathway : ErbB signaling pathway
WikiPathway : Myometrial Relaxation and Contraction Pathways

Clone

Cloning Information : 243
HIP Master Clone ID : 87207
Original Clone ID : FLH000243.01L

Organism


TAX_ID : 9606
Species Specific ID: 815

Genome

Chromosome : 5
Map Location : 5q32
Ensembl : ENSG00000070808

Reagents

Labome : CAMK2A-antibody