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GJA5 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GJA5
Gene Name:  gap junction protein, alpha 5, 40kDa
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH007603.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00021865

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1077nts         Open reading frame : 1 to 1077
Reference Sequence Alignment

ATGGGCGATTGGAGCTTCCTGGGAAATTTCCTGGAGGAAGTACACAAGCACTCGACCGTG
GTAGGCAAGGTCTGGCTCACTGTCCTCTTCATATTCCGTATGCTCGTGCTGGGCACAGCT
GCTGAGTCTTCCTGGGGGGATGAGCAGGCTGATTTCCGGTGTGATACGATTCAGCCTGGC
TGCCAGAATGTCTGCTACGACCAGGCTTTCCCCATCTCCCACATTCGCTACTGGGTGCTG
CAGATCATCTTCGTCTCCACGCCCTCTCTGGTGTACATGGGCCACGCCATGCACACTGTG
CGCATGCAGGAGAAGCGCAAGCTACGGGAGGCCGAGAGGGCCAAAGAGGTCCGGGGCTCT
GGCTCTTACGAGTACCCGGTGGCAGAGAAGGCAGAACTGTCCTGCTGGGAGGAAGGGAAT
GGAAGGATTGCCCTCCAGGGCACTCTGCTCAACACCTATGTGTGCAGCATCCTGATCCGC
ACCACCATGGAGGTGGGCTTCATTGTGGGCCAGTACTTCATCTACGGAATCTTCCTGACC
ACCCTGCATGTCTGCCGCAGGAGTCCCTGTCCCCACCCGGTCAACTGTTACGTATCCCGG
CCCACAGAGAAGAATGTCTTCATTGTCTTTATGCTGGCTGTGGCTGCACTGTCCCTCCTC
CTTAGCCTGGCTGAACTCTACCACCTGGGCTGGAAGAAGATCAGACAGCGATTTGTCAAA
CCGCGGCAGCACATGGCTAAGTGCCAGCTTTCTGGCCCCTCTGTGGGCATAGTCCAGAGC
TGCACACCACCCCCCGACTTTAATCAGTGCCTGGAGAATGGCCCTGGGGGAAAATTCTTC
AATCCCTTCAGCAATAATATGGCCTCCCAACAAAACACAGACAACCTGGTCACCGAGCAA
GTACGAGGTCAGGAGCAGACTCCTGGGGAAGGTTTCATCCAGGTTCGTTATGGCCAGAAG
CCTGAGGTGCCCAATGGAGTCTCACCAGGTCACCGCCTTCCCCATGGCTATCATAGTGAC
AAGCGACGTCTTAGTAAGGCCAGCAGCAAGGCAAGGGCAGATGACATATCAGTGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1077
Start on reference sequence 1
Mutations:
None
Discrepancy:
t1057g,S353A; c1066a,L356I
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GJA5
Symbol Nomenclature : GJA5
SYNONYM : ATFB11; CX40
Designation : connexin 40|gap junction alpha-5 protein
Full Nomenclature : gap junction protein, alpha 5, 40kDa
GENEID : 2702
GI : 61367848
GenBank Accession : AY891127
HGNC : 4279
MIM : 121013
Target GenBank: NM_005266

 

Reference Sequence Alignment

  

HsCD00021865      ATGGGCGATTGGAGCTTCCTGGGAAATTTCCTGGAGGAAGTACACAAGCACTCGACCGTG
NM_005266.6       ATGGGCGATTGGAGCTTCCTGGGAAATTTCCTGGAGGAAGTACACAAGCACTCGACCGTG
NM_181703.3       ATGGGCGATTGGAGCTTCCTGGGAAATTTCCTGGAGGAAGTACACAAGCACTCGACCGTG
                  ************************************************************

HsCD00021865      GTAGGCAAGGTCTGGCTCACTGTCCTCTTCATATTCCGTATGCTCGTGCTGGGCACAGCT
NM_005266.6       GTAGGCAAGGTCTGGCTCACTGTCCTCTTCATATTCCGTATGCTCGTGCTGGGCACAGCT
NM_181703.3       GTAGGCAAGGTCTGGCTCACTGTCCTCTTCATATTCCGTATGCTCGTGCTGGGCACAGCT
                  ************************************************************

HsCD00021865      GCTGAGTCTTCCTGGGGGGATGAGCAGGCTGATTTCCGGTGTGATACGATTCAGCCTGGC
NM_005266.6       GCTGAGTCTTCCTGGGGGGATGAGCAGGCTGATTTCCGGTGTGATACGATTCAGCCTGGC
NM_181703.3       GCTGAGTCTTCCTGGGGGGATGAGCAGGCTGATTTCCGGTGTGATACGATTCAGCCTGGC
                  ************************************************************

HsCD00021865      TGCCAGAATGTCTGCTACGACCAGGCTTTCCCCATCTCCCACATTCGCTACTGGGTGCTG
NM_005266.6       TGCCAGAATGTCTGCTACGACCAGGCTTTCCCCATCTCCCACATTCGCTACTGGGTGCTG
NM_181703.3       TGCCAGAATGTCTGCTACGACCAGGCTTTCCCCATCTCCCACATTCGCTACTGGGTGCTG
                  ************************************************************

HsCD00021865      CAGATCATCTTCGTCTCCACGCCCTCTCTGGTGTACATGGGCCACGCCATGCACACTGTG
NM_005266.6       CAGATCATCTTCGTCTCCACGCCCTCTCTGGTGTACATGGGCCACGCCATGCACACTGTG
NM_181703.3       CAGATCATCTTCGTCTCCACGCCCTCTCTGGTGTACATGGGCCACGCCATGCACACTGTG
                  ************************************************************

HsCD00021865      CGCATGCAGGAGAAGCGCAAGCTACGGGAGGCCGAGAGGGCCAAAGAGGTCCGGGGCTCT
NM_005266.6       CGCATGCAGGAGAAGCGCAAGCTACGGGAGGCCGAGAGGGCCAAAGAGGTCCGGGGCTCT
NM_181703.3       CGCATGCAGGAGAAGCGCAAGCTACGGGAGGCCGAGAGGGCCAAAGAGGTCCGGGGCTCT
                  ************************************************************

HsCD00021865      GGCTCTTACGAGTACCCGGTGGCAGAGAAGGCAGAACTGTCCTGCTGGGAGGAAGGGAAT
NM_005266.6       GGCTCTTACGAGTACCCGGTGGCAGAGAAGGCAGAACTGTCCTGCTGGGAGGAAGGGAAT
NM_181703.3       GGCTCTTACGAGTACCCGGTGGCAGAGAAGGCAGAACTGTCCTGCTGGGAGGAAGGGAAT
                  ************************************************************

HsCD00021865      GGAAGGATTGCCCTCCAGGGCACTCTGCTCAACACCTATGTGTGCAGCATCCTGATCCGC
NM_005266.6       GGAAGGATTGCCCTCCAGGGCACTCTGCTCAACACCTATGTGTGCAGCATCCTGATCCGC
NM_181703.3       GGAAGGATTGCCCTCCAGGGCACTCTGCTCAACACCTATGTGTGCAGCATCCTGATCCGC
                  ************************************************************

HsCD00021865      ACCACCATGGAGGTGGGCTTCATTGTGGGCCAGTACTTCATCTACGGAATCTTCCTGACC
NM_005266.6       ACCACCATGGAGGTGGGCTTCATTGTGGGCCAGTACTTCATCTACGGAATCTTCCTGACC
NM_181703.3       ACCACCATGGAGGTGGGCTTCATTGTGGGCCAGTACTTCATCTACGGAATCTTCCTGACC
                  ************************************************************

HsCD00021865      ACCCTGCATGTCTGCCGCAGGAGTCCCTGTCCCCACCCGGTCAACTGTTACGTATCCCGG
NM_005266.6       ACCCTGCATGTCTGCCGCAGGAGTCCCTGTCCCCACCCGGTCAACTGTTACGTATCCCGG
NM_181703.3       ACCCTGCATGTCTGCCGCAGGAGTCCCTGTCCCCACCCGGTCAACTGTTACGTATCCCGG
                  ************************************************************

HsCD00021865      CCCACAGAGAAGAATGTCTTCATTGTCTTTATGCTGGCTGTGGCTGCACTGTCCCTCCTC
NM_005266.6       CCCACAGAGAAGAATGTCTTCATTGTCTTTATGCTGGCTGTGGCTGCACTGTCCCTCCTC
NM_181703.3       CCCACAGAGAAGAATGTCTTCATTGTCTTTATGCTGGCTGTGGCTGCACTGTCCCTCCTC
                  ************************************************************

HsCD00021865      CTTAGCCTGGCTGAACTCTACCACCTGGGCTGGAAGAAGATCAGACAGCGATTTGTCAAA
NM_005266.6       CTTAGCCTGGCTGAACTCTACCACCTGGGCTGGAAGAAGATCAGACAGCGATTTGTCAAA
NM_181703.3       CTTAGCCTGGCTGAACTCTACCACCTGGGCTGGAAGAAGATCAGACAGCGATTTGTCAAA
                  ************************************************************

HsCD00021865      CCGCGGCAGCACATGGCTAAGTGCCAGCTTTCTGGCCCCTCTGTGGGCATAGTCCAGAGC
NM_005266.6       CCGCGGCAGCACATGGCTAAGTGCCAGCTTTCTGGCCCCTCTGTGGGCATAGTCCAGAGC
NM_181703.3       CCGCGGCAGCACATGGCTAAGTGCCAGCTTTCTGGCCCCTCTGTGGGCATAGTCCAGAGC
                  ************************************************************

HsCD00021865      TGCACACCACCCCCCGACTTTAATCAGTGCCTGGAGAATGGCCCTGGGGGAAAATTCTTC
NM_005266.6       TGCACACCACCCCCCGACTTTAATCAGTGCCTGGAGAATGGCCCTGGGGGAAAATTCTTC
NM_181703.3       TGCACACCACCCCCCGACTTTAATCAGTGCCTGGAGAATGGCCCTGGGGGAAAATTCTTC
                  ************************************************************

HsCD00021865      AATCCCTTCAGCAATAATATGGCCTCCCAACAAAACACAGACAACCTGGTCACCGAGCAA
NM_005266.6       AATCCCTTCAGCAATAATATGGCCTCCCAACAAAACACAGACAACCTGGTCACCGAGCAA
NM_181703.3       AATCCCTTCAGCAATAATATGGCCTCCCAACAAAACACAGACAACCTGGTCACCGAGCAA
                  ************************************************************

HsCD00021865      GTACGAGGTCAGGAGCAGACTCCTGGGGAAGGTTTCATCCAGGTTCGTTATGGCCAGAAG
NM_005266.6       GTACGAGGTCAGGAGCAGACTCCTGGGGAAGGTTTCATCCAGGTTCGTTATGGCCAGAAG
NM_181703.3       GTACGAGGTCAGGAGCAGACTCCTGGGGAAGGTTTCATCCAGGTTCGTTATGGCCAGAAG
                  ************************************************************

HsCD00021865      CCTGAGGTGCCCAATGGAGTCTCACCAGGTCACCGCCTTCCCCATGGCTATCATAGTGAC
NM_005266.6       CCTGAGGTGCCCAATGGAGTCTCACCAGGTCACCGCCTTCCCCATGGCTATCATAGTGAC
NM_181703.3       CCTGAGGTGCCCAATGGAGTCTCACCAGGTCACCGCCTTCCCCATGGCTATCATAGTGAC
                  ************************************************************

HsCD00021865      AAGCGACGTCTTAGTAAGGCCAGCAGCAAGGCAAGGGCAGATGACATATCAGTGTTG
NM_005266.6       AAGCGACGTCTTAGTAAGGCCAGCAGCAAGGCAAGGTCAGATGACCTATCAGTGTGA
NM_181703.3       AAGCGACGTCTTAGTAAGGCCAGCAGCAAGGCAAGGTCAGATGACCTATCAGTGTGA
                  ************************************ ********.********* .

Pathways

Reactome : Gap junction assembly
Reactome : Gap junction trafficking
Reactome : Gap junction trafficking and regulation
Reactome : Membrane Trafficking
WikiPathway : Calcium Regulation in the Cardiac Cell

Clone

Cloning Information : 7603
HIP Master Clone ID : 7161
Original Clone ID : FLH007603.01L

Organism


TAX_ID : 9606
Species Specific ID: 2702

Genome

Chromosome : 1
Map Location : 1q21.1
Ensembl : ENSG00000143140

Reagents

Labome : GJA5-antibody