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CETP (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CETP
Gene Name:  cholesteryl ester transfer protein, plasma
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH008682.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00021891

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1302nts         Open reading frame : 1 to 1302
Reference Sequence Alignment

ATGCTGGCTGCCACAGTCCTGACCCTGGCCCTGCTGGGCAATGCCCATGCCTGCTCCAAA
GGCACCTCGCACGAGGCAGGCATCGTGTGCCGCATCACCAAGCCTGCCCTCCTGGTGTTG
AACCACGAGACTGCCAAGGTGATCCAGACCGCCTTCCAGCGAGCCAGCTACCCAGATATC
ACGGGCGAGAAGGCCATGATGCTCCTTGGCCAAGTCAAGTATGGGTTGCACAACATCCAG
ATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGAT
GTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACT
GCCTGGTGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTC
CAGATCAACACACAGCTGACCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGC
TACCTGTCTTTCCATAAGCTGCTCCTGCATCTCCAAGGGGAGCGAGAGCCTGGGTGGATC
AAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGATC
TGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGCC
AGCATCCTTTCAGATGGAGACATTGGGGTGGACATTTCCCTGACAGGTGATCCCGTCATC
ACAGCCTCCTACCTGGAGTCCCATCACAAGGCAGTGCTGGAGACCTGGGGCTTCAACACC
AACCAGGAAATCTTCCAAGAGGTTGTCGGCGGCTTCCCCAGCCAGGCCCAAGTCACCGTC
CACTGCCTCAAGATGCCCAAGATCTCCTGCCAAAACAAGGGAGTCGTGGTCAATTCTTCA
GTGATGGTGAAATTCCTCTTTCCACGCCCAGACCAGCAACATTCTGTAGCTTACACATTT
GAAGAGGATATCGTGACTACCGTCCAGGCCTCCTATTCTAAGAAAAAGCTCTTCTTAAGC
CTCTTGGATTTCCAGATTACACCAAAGACTGTTTCCAACTTGACTGAGAGCAGCTCCGAG
TCCATCCAGAGCTTCCTGCAGTCAATGATCACCGCTGTGGGCATCCCTGAGGTCATGTCT
CGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATC
ATCAACCCTGAGATTATCACTCGAGATGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTC
CCTGAGCACCTGCTGGTGGATTTCCTCAAGAGCTTGAGCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1612
Start on reference sequence 131
Mutations:
None
Discrepancy:
del@747,180;c1468a,Q490K;
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CETP
Symbol Nomenclature : CETP
SYNONYM : BPIFF; HDLCQ10
Designation : BPI fold containing family F|cholesteryl ester transfer protein|lipid transfer protein I
Full Nomenclature : cholesteryl ester transfer protein, plasma
GENEID : 1071
HGNC : 1869
MIM : 118470
Vega : OTTHUMG00000133279
Target GenBank: NM_000078

 

Reference Sequence Alignment

  

HsCD00021891        ATGCTGGCTGCCACAGTCCTGACCCTGGCCCTGCTGGGCAATGCCCATGCCTGCTCCAAA
NM_001286085.1      ATGCTGGCTGCCACAGTCCTGACCCTGGCCCTGCTGGGCAATGCCCATGCCTGCTCCAAA
                    ************************************************************

HsCD00021891        GGCACCTCGCACGAGGCAGGCATCGTGTGCCGCATCACCAAGCCTGCCCTCCTGGTGTTG
NM_001286085.1      GGCACCTCGCACGAGGCAGGCATCGTGTGCCGCATCACCAAGCCTGCCCTCCTGGTGTTG
                    ************************************************************

HsCD00021891        AACCACGAGACTGCCAAGGTGATCCAGACCGCCTTCCAGCGAGCCAGCTACCCAGATATC
NM_001286085.1      AACCACGAGACTGCCAAGGTGATCCAGACCGCCTTCCAGCGAGCCAGCTACCCAGATATC
                    ************************************************************

HsCD00021891        ACGGGCGAGAAGGCCATGATGCTCCTTGGCCAAGTCAAGTATGGGTTGCACAACATCCAG
NM_001286085.1      ACGGGCGAGAAGGCCATGATGCTCCTTGGCCAAGTCAAGTATGGGTTGCACAACATCCAG
                    ************************************************************

HsCD00021891        ATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGAT
NM_001286085.1      ATCAGCCACTTGTCCATCGCCAGCAGCCAGGTGGAGCTGGTGGAAGCCAAGTCCATTGAT
                    ************************************************************

HsCD00021891        GTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACT
NM_001286085.1      GTCTCCATTCAGAACGTGTCTGTGGTCTTCAAGGGGACCCTGAAGTATGGCTACACCACT
                    ************************************************************

HsCD00021891        GCCTGGTGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTC
NM_001286085.1      GCCTGGTGGCTGGGTATTGATCAGTCCATTGACTTCGAGATCGACTCTGCCATTGACCTC
                    ************************************************************

HsCD00021891        CAGATCAACACACAGCTGACCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGC
NM_001286085.1      CAGATCAACACACAGCTGACCTGTGACTCTGGTAGAGTGCGGACCGATGCCCCTGACTGC
                    ************************************************************

HsCD00021891        TACCTGTCTTTCCATAAGCTGCTCCTGCATCTCCAAGGGGAGCGAGAGCCTGGGTGGATC
NM_001286085.1      TACCTGTCTTTCCATAAGCTGCTCCTGCATCTCCAAGGGGAGCGAGAGCCTGGGTGGATC
                    ************************************************************

HsCD00021891        AAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGATC
NM_001286085.1      AAGCAGCTGTTCACAAATTTCATCTCCTTCACCCTGAAGCTGGTCCTGAAGGGACAGATC
                    ************************************************************

HsCD00021891        TGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGCC
NM_001286085.1      TGCAAAGAGATCAACGTCATCTCTAACATCATGGCCGATTTTGTCCAGACAAGGGCTGCC
                    ************************************************************

HsCD00021891        AGCATCCTTTCAGATGGAGACATTGGGGTGGACATTTCCCTGACAGGTGATCCCGTCATC
NM_001286085.1      AGCATCCTTTCAGATGGAGACATTGGGGTGGACATTTCCCTGACAGGTGATCCCGTCATC
                    ************************************************************

HsCD00021891        ACAGCCTCCTACCTGGAGTCCCATCACAAGGCAGTGCTGGAGACCTGGGGCTTCAACACC
NM_001286085.1      ACAGCCTCCTACCTGGAGTCCCATCACAAGGCAGTGCTGGAGACCTGGGGCTTCAACACC
                    ************************************************************

HsCD00021891        AACCAGGAAATCTTCCAAGAGGTTGTCGGCGGCTTCCCCAGCCAGGCCCAAGTCACCGTC
NM_001286085.1      AACCAGGAAATCTTCCAAGAGGTTGTCGGCGGCTTCCCCAGCCAGGCCCAAGTCACCGTC
                    ************************************************************

HsCD00021891        CACTGCCTCAAGATGCCCAAGATCTCCTGCCAAAACAAGGGAGTCGTGGTCAATTCTTCA
NM_001286085.1      CACTGCCTCAAGATGCCCAAGATCTCCTGCCAAAACAAGGGAGTCGTGGTCAATTCTTCA
                    ************************************************************

HsCD00021891        GTGATGGTGAAATTCCTCTTTCCACGCCCAGACCAGCAACATTCTGTAGCTTACACATTT
NM_001286085.1      GTGATGGTGAAATTCCTCTTTCCACGCCCAGACCAGCAACATTCTGTAGCTTACACATTT
                    ************************************************************

HsCD00021891        GAAGAGGATATCGTGACTACCGTCCAGGCCTCCTATTCTAAGAAAAAGCTCTTCTTAAGC
NM_001286085.1      GAAGAGGATATCGTGACTACCGTCCAGGCCTCCTATTCTAAGAAAAAGCTCTTCTTAAGC
                    ************************************************************

HsCD00021891        CTCTTGGATTTCCAGATTACACCAAAGACTGTTTCCAACTTGACTGAGAGCAGCTCCGAG
NM_001286085.1      CTCTTGGATTTCCAGATTACACCAAAGACTGTTTCCAACTTGACTGAGAGCAGCTCCGAG
                    ************************************************************

HsCD00021891        TCCATCCAGAGCTTCCTGCAGTCAATGATCACCGCTGTGGGCATCCCTGAGGTCATGTCT
NM_001286085.1      TCCGTCCAGAGCTTCCTGCAGTCAATGATCACCGCTGTGGGCATCCCTGAGGTCATGTCT
                    ***.********************************************************

HsCD00021891        CGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATC
NM_001286085.1      CGGCTCGAGGTAGTGTTTACAGCCCTCATGAACAGCAAAGGCGTGAGCCTCTTCGACATC
                    ************************************************************

HsCD00021891        ATCAACCCTGAGATTATCACTCGAGATGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTC
NM_001286085.1      ATCAACCCTGAGATTATCACTCGAGATGGCTTCCTGCTGCTGCAGATGGACTTTGGCTTC
                    ************************************************************

HsCD00021891        CCTGAGCACCTGCTGGTGGATTTCCTCAAGAGCTTGAGCTAG
NM_001286085.1      CCTGAGCACCTGCTGGTGGATTTCCTCCAGAGCTTGAGCTAG
                    ***************************.**************

Pathways

Reactome : HDL-mediated lipid transport
Reactome : LDL-mediated lipid transport
Reactome : Lipid digestion, mobilization, and transport
Reactome : Lipoprotein metabolism
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
WikiPathway : Statin Pathway

Protein

SMART domain : BPI1 : BPI/LBP/CETP N-terminal domain
SMART domain : BPI2 : BPI/LBP/CETP C-terminal domain
UniProt : P11597
HPRD : 00325

Clone

Cloning Information : 8682
HIP Master Clone ID : 11177
Original Clone ID : FLH008682.01X

Organism


TAX_ID : 9606
Species Specific ID: 1071

Genome

Chromosome : 16
Map Location : 16q21
Ensembl : ENSG00000087237

Reagents

Labome : CETP-antibody