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TNFRSF10B (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TNFRSF10B
Gene Name:  tumor necrosis factor receptor superfamily, member 10b
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH019317.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00022105

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1236nts         Open reading frame : 1 to 1236
Reference Sequence Alignment

ATGGAACAACGGGGACAGAACGCCCCGGCCGCTTCGGGGGCCCGGAAAAGGCACGGCCCA
GGACCCAGGGAGGCGCGGGGAGCCAGGCCTGGGCTCCGGGTCCCCAAGACCCTTGTGCTC
GTTGTCGCCGCGGTCCTGCTGTTGGTCTCAGCTGAGTCTGCTCTGATCACCCAACAAGAC
CTAGCTCCCCAGCAGAGAGCGGCCCCACAACAAAAGAGGTCCAGCCCCTCAGAGGGATTG
TGTCCACCTGGACACCATATCTCAGAAGACGGTAGAGATTGCATCTCCTGCAAATATGGA
CAGGACTATAGCACTCACTGGAATGACCTCCTTTTCTGCTTGCGCTGCACCAGGTGTGAT
TCAGGTGAAGTGGAGCTAAGTCCCTGCACCACGACCAGAAACACAGTGTGTCAGTGCGAA
GAAGGCACCTTCCGGGAAGAAGATTCTCCTGAGATGTGCCGGAAGTGCCGCACAGGGTGT
CCCAGAGGGATGGTCAAGGTCGGTGATTGTACACCCTGGAGTGACATCGAATGTGTCCAC
AAAGAATCAGGCATCATCATAGGAGTCACAGTTGCAGCCGTAGTCTTGATTGTGGCTGTG
TTTGTTTGCAAGTCTTTACTGTGGAAGAAAGTCCTTCCTTACCTGAAAGGCATCTGCTCA
GGTGGTGATGGGGACCCTGAGCGTGTGGACAGAAGCTCACAACGACCTGGGGCTGAGGAC
AATGTCCTCAATGAGATCGTGAGTATCTTGCAGCCCACCCAGGTCCCTGAGCAGGAAATG
GAAGTCCAGGAGCCAGCAGAGCCAACAGGTGTCAACATGTTGTCCCCCGGGGAGTCAGAG
CATCTGCTGGAACCGGCAGAAGCTGAAAGGTCTCAGAGGAGGAGGCTGCTGGTTCCAGCA
AATGAAGGTGATCCCACTGAGACTCTGAGACAGTGCTTCGATGACTTTGCAGACTTGGTG
CCCTTTGACTCCTGGGAGCCGCTCATGAGGAAGTTGGGCCTCATGGACAATGAGATAAAG
GTGGCTAAAGCTGAGGCAGCGGGCCACAGGGACACCTTGTACACGATGCTGATAAAGTGG
GTCAACAAAACCGGGCGAGATGCCTCTGTCCACACCCTGCTGGATGCCTTGGAGACGCTG
GGAGAGAGACTTGCCAAGCAGAAGATTGAGGACCACTTGTTGAGCTCTGGAAAGTTCATG
TATCTAGAAGGTAATGCAGACTCTGCCATGTCCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1236
Start on reference sequence 1
Mutations:
None
Discrepancy:
c95t,P32L; g668a,G223D
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TNFRSF10B
Symbol Nomenclature : TNFRSF10B
SYNONYM : CD262; DR5; KILLER; KILLER/DR5; TRAIL-R2; TRAILR2; TRICK2; TRICK2A; TRICK2B; TRICKB; ZTNFR9
Designation : Fas-like protein|TNF receptor superfamily member 10b|TNF-related apoptosis-inducing ligand receptor 2|apoptosis inducing protein TRICK2A/2B|apoptosis inducing receptor TRAIL-R2|cytotoxic TRAIL receptor-2|death domain containing receptor for TRAIL/Apo-2L|death receptor 5|p53-regulated DNA damage-inducible cell death receptor(killer)|tumor necrosis factor receptor superfamily member 10B|tumor necrosis factor receptor-like protein ZTNFR9
Full Nomenclature : tumor necrosis factor receptor superfamily, member 10b
GENEID : 8795
Locus Tag : UNQ160/PRO186
GI : 61364961
GenBank Accession : AY890690
HGNC : 11905
MIM : 603612
Vega : OTTHUMG00000097826
Target GenBank: NM_003842

 

Reference Sequence Alignment

  

HsCD00022105      ATGGAACAACGGGGACAGAACGCCCCGGCCGCTTCGGGGGCCCGGAAAAGGCACGGCCCA
NM_147187.2       ATGGAACAACGGGGACAGAACGCCCCGGCCGCTTCGGGGGCCCGGAAAAGGCACGGCCCA
                  ************************************************************

HsCD00022105      GGACCCAGGGAGGCGCGGGGAGCCAGGCCTGGGCTCCGGGTCCCCAAGACCCTTGTGCTC
NM_147187.2       GGACCCAGGGAGGCGCGGGGAGCCAGGCCTGGGCCCCGGGTCCCCAAGACCCTTGTGCTC
                  ********************************** *************************

HsCD00022105      GTTGTCGCCGCGGTCCTGCTGTTGGTCTCAGCTGAGTCTGCTCTGATCACCCAACAAGAC
NM_147187.2       GTTGTCGCCGCGGTCCTGCTGTTGGTCTCAGCTGAGTCTGCTCTGATCACCCAACAAGAC
                  ************************************************************

HsCD00022105      CTAGCTCCCCAGCAGAGAGCGGCCCCACAACAAAAGAGGTCCAGCCCCTCAGAGGGATTG
NM_147187.2       CTAGCTCCCCAGCAGAGAGCGGCCCCACAACAAAAGAGGTCCAGCCCCTCAGAGGGATTG
                  ************************************************************

HsCD00022105      TGTCCACCTGGACACCATATCTCAGAAGACGGTAGAGATTGCATCTCCTGCAAATATGGA
NM_147187.2       TGTCCACCTGGACACCATATCTCAGAAGACGGTAGAGATTGCATCTCCTGCAAATATGGA
                  ************************************************************

HsCD00022105      CAGGACTATAGCACTCACTGGAATGACCTCCTTTTCTGCTTGCGCTGCACCAGGTGTGAT
NM_147187.2       CAGGACTATAGCACTCACTGGAATGACCTCCTTTTCTGCTTGCGCTGCACCAGGTGTGAT
                  ************************************************************

HsCD00022105      TCAGGTGAAGTGGAGCTAAGTCCCTGCACCACGACCAGAAACACAGTGTGTCAGTGCGAA
NM_147187.2       TCAGGTGAAGTGGAGCTAAGTCCCTGCACCACGACCAGAAACACAGTGTGTCAGTGCGAA
                  ************************************************************

HsCD00022105      GAAGGCACCTTCCGGGAAGAAGATTCTCCTGAGATGTGCCGGAAGTGCCGCACAGGGTGT
NM_147187.2       GAAGGCACCTTCCGGGAAGAAGATTCTCCTGAGATGTGCCGGAAGTGCCGCACAGGGTGT
                  ************************************************************

HsCD00022105      CCCAGAGGGATGGTCAAGGTCGGTGATTGTACACCCTGGAGTGACATCGAATGTGTCCAC
NM_147187.2       CCCAGAGGGATGGTCAAGGTCGGTGATTGTACACCCTGGAGTGACATCGAATGTGTCCAC
                  ************************************************************

HsCD00022105      AAAGAATCAGGCATCATCATAGGAGTCACAGTTGCAGCCGTAGTCTTGATTGTGGCTGTG
NM_147187.2       AAAGAATCAGGCATCATCATAGGAGTCACAGTTGCAGCCGTAGTCTTGATTGTGGCTGTG
                  ************************************************************

HsCD00022105      TTTGTTTGCAAGTCTTTACTGTGGAAGAAAGTCCTTCCTTACCTGAAAGGCATCTGCTCA
NM_147187.2       TTTGTTTGCAAGTCTTTACTGTGGAAGAAAGTCCTTCCTTACCTGAAAGGCATCTGCTCA
                  ************************************************************

HsCD00022105      GGTGGTGATGGGGACCCTGAGCGTGTGGACAGAAGCTCACAACGACCTGGGGCTGAGGAC
NM_147187.2       GGTGGTGGTGGGGACCCTGAGCGTGTGGACAGAAGCTCACAACGACCTGGGGCTGAGGAC
                  *******.****************************************************

HsCD00022105      AATGTCCTCAATGAGATCGTGAGTATCTTGCAGCCCACCCAGGTCCCTGAGCAGGAAATG
NM_147187.2       AATGTCCTCAATGAGATCGTGAGTATCTTGCAGCCCACCCAGGTCCCTGAGCAGGAAATG
                  ************************************************************

HsCD00022105      GAAGTCCAGGAGCCAGCAGAGCCAACAGGTGTCAACATGTTGTCCCCCGGGGAGTCAGAG
NM_147187.2       GAAGTCCAGGAGCCAGCAGAGCCAACAGGTGTCAACATGTTGTCCCCCGGGGAGTCAGAG
                  ************************************************************

HsCD00022105      CATCTGCTGGAACCGGCAGAAGCTGAAAGGTCTCAGAGGAGGAGGCTGCTGGTTCCAGCA
NM_147187.2       CATCTGCTGGAACCGGCAGAAGCTGAAAGGTCTCAGAGGAGGAGGCTGCTGGTTCCAGCA
                  ************************************************************

HsCD00022105      AATGAAGGTGATCCCACTGAGACTCTGAGACAGTGCTTCGATGACTTTGCAGACTTGGTG
NM_147187.2       AATGAAGGTGATCCCACTGAGACTCTGAGACAGTGCTTCGATGACTTTGCAGACTTGGTG
                  ************************************************************

HsCD00022105      CCCTTTGACTCCTGGGAGCCGCTCATGAGGAAGTTGGGCCTCATGGACAATGAGATAAAG
NM_147187.2       CCCTTTGACTCCTGGGAGCCGCTCATGAGGAAGTTGGGCCTCATGGACAATGAGATAAAG
                  ************************************************************

HsCD00022105      GTGGCTAAAGCTGAGGCAGCGGGCCACAGGGACACCTTGTACACGATGCTGATAAAGTGG
NM_147187.2       GTGGCTAAAGCTGAGGCAGCGGGCCACAGGGACACCTTGTACACGATGCTGATAAAGTGG
                  ************************************************************

HsCD00022105      GTCAACAAAACCGGGCGAGATGCCTCTGTCCACACCCTGCTGGATGCCTTGGAGACGCTG
NM_147187.2       GTCAACAAAACCGGGCGAGATGCCTCTGTCCACACCCTGCTGGATGCCTTGGAGACGCTG
                  ************************************************************

HsCD00022105      GGAGAGAGACTTGCCAAGCAGAAGATTGAGGACCACTTGTTGAGCTCTGGAAAGTTCATG
NM_147187.2       GGAGAGAGACTTGCCAAGCAGAAGATTGAGGACCACTTGTTGAGCTCTGGAAAGTTCATG
                  ************************************************************

HsCD00022105      TATCTAGAAGGTAATGCAGACTCTGCCATGTCCTTG
NM_147187.2       TATCTAGAAGGTAATGCAGACTCTGCCATGTCCTAA
                  **********************************:.

Pathways

NCI : Caspase Cascade in Apoptosis
NCI : Direct p53 effectors
NCI : TRAIL signaling pathway
Panther : Apoptosis signaling pathway
Panther : p53 pathway
Reactome : Apoptosis
Reactome : Caspase-8 activation
Reactome : Death Receptor Signalling
Reactome : Dimerization of procaspase-8
Reactome : Extrinsic Pathway for Apoptosis
Reactome : Regulation by c-FLIP
Reactome : TRAIL signaling
WikiPathway : Apoptosis
WikiPathway : Apoptosis Modulation and Signaling
WikiPathway : DNA damage response
WikiPathway : Epithelium TarBase
WikiPathway : Lymphocyte TarBase
WikiPathway : Muscle cell TarBase
WikiPathway : Squamous cell TarBase
WikiPathway : miRNA regulation of DNA Damage Response

Clone

Cloning Information : 19317
HIP Master Clone ID : 33516
Original Clone ID : FLH019317.01L

Organism


TAX_ID : 9606
Species Specific ID: 8795

Genome

Chromosome : 8
Map Location : 8p22-p21
Ensembl : ENSG00000120889

Reagents

Labome : DR5-antibody