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NUPL2 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  NUPL2
Gene Name:  nucleoporin like 2
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH023461.01X
Keyword: potential short variant
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: Short variant or partial clone comparing to NM_007342
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00022178

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 675nts         Open reading frame : 1 to 675
Reference Sequence Alignment

ATGGCCATTTGTCAATTCTTCCTTCAAGGCCGGTGCCGCTTTGGAGATCGGTGCTGGAAC
GAACATCCCGGTGCTAGGGGTGCAGGAGGAGGACGGCAGCAACCGCAGCAGCAGCCTTCA
GGTAATAATAGACGTGGATGGAATACAACTAGCCAGAGATATTCCAATGTCATCCAGCCA
TCCAGTTTCTCCAAATCCACACCATGGGGGGGCAGCAGAGATCAAGAAAAGCCATATTTC
AGTTCTTTTGATTCTGGAGCTTCAACTAACAGGAAGGAAGGCTTTGGATTGTCTGAGAAC
CCATTTGCTTCACTTAGTCCTGATGAGCAGAAAGATGAAAAGAAACTTCTGGAAGGAATT
GTAAAAGATATGGAGGTTTGGGAATCATCAGGGCAGTGGATGTTTTCTGTTTATTCACCA
GTGAAAAAGAAACCTAATATTTCAGGTTTTACAGACATTTCACCAGAGGAATTGAGGCTT
GAATACCATAACTTCTTAACCAGCAATAACTTACAGAGTTATCTAAATTCTGTCCAACGT
TTAATAAATCAATGGAGGAACAGGGTAAATGAACTGAAAAGTCTAAATATATCAACTAAA
GTAGCTTTGAAATGCCTTTGGAAGATATATACCCACCTGTCTCCAAAGATCTATGGTCCC
GACTGTGCTCTCTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 737
Start on reference sequence 63
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NUPL2
Symbol Nomenclature : NUPL2
SYNONYM : CG1; NLP-1; NLP_1; hCG1
Designation : H_RG271G13.9|NUP42 homolog|nucleoporin hCG1|nucleoporin-like protein 1|nucleoporin-like protein 2
Full Nomenclature : nucleoporin like 2
GENEID : 11097
HGNC : 17010
Vega : OTTHUMG00000096955
Target GenBank: BC005327

 

Reference Sequence Alignment

  

HsCD00022178        ATGGCCATTTGTCAATTCTTCCTTCAAGGCCGGTGCCGCTTTGGAGATCGGTGCTGGAAC
XM_005249592.1      ATGGCCATTTGTCAATTCTTCCTTCAAGGCCGGTGCCGCTTTGGAGATCGGTGCTGGAAC
NM_007342.2         ATGGCCATTTGTCAATTCTTCCTTCAAGGCCGGTGCCGCTTTGGAGATCGGTGCTGGAAC
                    ************************************************************

HsCD00022178        GAACATCCCGGTGCTAGGGGTGCAGGAGGAGGACGGCAGCAACCGCAGCAGCAGCCTTCA
XM_005249592.1      GAACATCCCGGTGCTAGGGGTGCAGGAGGAGGACGGCAGCAACCGCAGCAGCAGCCTTCA
NM_007342.2         GAACATCCCGGTGCTAGGGGTGCAGGAGGAGGACGGCAGCAACCGCAGCAGCAGCCTTCA
                    ************************************************************

HsCD00022178        GGTAATAATAGACGTGGATGGAATACAACTAGCCAGAGATATTCCAATGTCATCCAGCCA
XM_005249592.1      GGTAATAATAGACGTGGATGGAATACAACTAGCCAGAGATATTCCAATGTCATCCAGCCA
NM_007342.2         GGTAATAATAGACGTGGATGGAATACAACTAGCCAGAGATATTCCAATGTCATCCAGCCA
                    ************************************************************

HsCD00022178        TCCAGTTTCTCCAAATCCACACCATGGGGGGGCAGCAGAGATCAAGAAAAGCCATATTTC
XM_005249592.1      TCCAGTTTCTCCAAATCCACACCATGGGGGGGCAGCAGAGATCAAGAAAAGCCATATTTC
NM_007342.2         TCCAGTTTCTCCAAATCCACACCATGGGGGGGCAGCAGAGATCAAGAAAAGCCATATTTC
                    ************************************************************

HsCD00022178        AGTTCTTTTGATTCTGGAGCTTCAACTAACAGGAAGGAAGGCTTTGGATTGTCTGAGAAC
XM_005249592.1      AGTTCTTTTGATTCTGGAGCTTCAACTAACAGGAAGGAAGGCTTTGGATTGTCTGAGAAC
NM_007342.2         AGTTCTTTTGATTCTGGAGCTTCAACTAACAGGAAGGAAGGCTTTGGATTGTCTGAGAAC
                    ************************************************************

HsCD00022178        CCATTTGCTTCACTTAGTCCTGATGAGCAGAAAGATGAAAAGAAACTTCTGGAAGGAATT
XM_005249592.1      CCATTTGCTTCACTTAGTCCTGATGAGCAGAAAGATGAAAAGAAACTTCTGGAAGGAATT
NM_007342.2         CCATTTGCTTCACTTAGTCCTGATGAGCAGAAAGATGAAAAGAAACTTCTGGAAGGAATT
                    ************************************************************

HsCD00022178        GTAAAAGATATGGAGGTTTGGGAATCATCAGGGCAGTGGATGTTTTCTGTTTATTCACCA
XM_005249592.1      GTAAAAGATATGGAGGTTTGGGAATCATCAGGGCAGTGGATGTTTTCTGTTTATTCACCA
NM_007342.2         GTAAAAGATATGGAGGTTTGGGAATCATCAGGGCAGTGGATGTTTTCTGTTTATTCACCA
                    ************************************************************

HsCD00022178        GTGAAAAAGAAACCTAATATTTCAGGTTTTACAGACATTTCACCAGAGGAATTGAGGCTT
XM_005249592.1      GTGAAAAAGAAACCTAATATTTCAGGTTTTACAGACATTTCACCAGAGGAATTGAGGCTT
NM_007342.2         GTGAAAAAGAAACCTAATATTTCAGGTTTTACAGACATTTCACCAGAGGAATTGAGGCTT
                    ************************************************************

HsCD00022178        GAATACCATAACTTCTTAACCAGCAATAACTTACAGAGTTATCTAAATTCTGTCCAACGT
XM_005249592.1      GAATACCATAACTTCTTAACCAGCAATAACTTACAGAGTTATCTAAATTCTGTCCAACGT
NM_007342.2         GAATACCATAACTTCTTAACCAGCAATAACTTACAGAGTTATCTAAATTCTGTCCAACGT
                    ************************************************************

HsCD00022178        TTAATAAATCAATGGAGGAACAGGGTAAATGAACTGAAAAGTCTAAATATATCAACTAAA
XM_005249592.1      TTAATAAATCAATGGAGGAACAGGGTAAATGAACTGAAAAGTCTAAATATATCAACTAAA
NM_007342.2         TTAATAAATCAATGGAGGAACAGGGTAAATGAACTGAAAAGTCTAAATATATCAACTAAA
                    ************************************************************

HsCD00022178        GTAGCTTTG---------------------------------------------------
XM_005249592.1      GTAGCTTTGAAATGCCTTTGGAAGATATATACCCACCTGTCTCCAAAGATCTATGGTCCC
NM_007342.2         GTAGCTTT----------------------------------------------------
                    ********                                                    

HsCD00022178        -----------------------------------------------------AAATGCC
XM_005249592.1      GACTGTGGCAAGTTCATTATCTTTCTCTCTGATGTAAAGGATGGAGTAAATCAAGCAGCA
NM_007342.2         -----------------------GCTCTCTGATGTAAAGGATGGAGTAAATCAAGCAGCA
                                                                         *..:**.

HsCD00022178        TTTGGAAGATATATACCCACCTGTCTCCAAAGATCTATGGTCCCGACTGTGCTCTCTAG-
XM_005249592.1      CCTGCATTTGGATTTGGCAGCAGTCAAGCAGCAACATTTATGTCGCCAGGCTTTCCAGTC
NM_007342.2         CCTGCATTTGGATTTGGCAGCAGTCAAGCAGCAACATTTATGTCGCCAGGCTTTCCAGTC
                      ** *: : .::*:  ** *:***:. .*. *:*::* .*  **.*:*   *  *:.  

HsCD00022178        ------------------------------------------------------------
XM_005249592.1      AATAACAGCAGCAGTGATAATGCTCAGAACTTTAGTTTTAAAACAAACTCTGGATTTGCT
NM_007342.2         AATAACAGCAGCAGTGATAATGCTCAGAACTTTAGTTTTAAAACAAACTCTGGATTTGCT
                                                                                

HsCD00022178        ------------------------------------------------------------
XM_005249592.1      GCTGCCTCTTCTGGAAGCCCTGCTGGTTTTGGGAGTTCCCCAGCATTTGGAGCTGCAGCC
NM_007342.2         GCTGCCTCTTCTGGAAGCCCTGCTGGTTTTGGGAGTTCCCCAGCATTTGGAGCTGCAGCC
                                                                                

HsCD00022178        ------------------------------------------------------------
XM_005249592.1      TCTACCAGTTCAGGTATCTCTACTTCTGCTCCAGCTTTTGGATTTGGGAAGCCTGAAGTC
NM_007342.2         TCTACCAGTTCAGGTATCTCTACTTCTGCTCCAGCTTTTGGATTTGGGAAGCCTGAAGTC
                                                                                

HsCD00022178        ------------------------------------------------------------
XM_005249592.1      ACATCGGCTGCATCATTTTCATTCAAAAGCCCTGCAGCTTCCAGTTTTGGATCACCTGGA
NM_007342.2         ACATCGGCTGCATCATTTTCATTCAAAAGCCCTGCAGCTTCCAGTTTTGGATCACCTGGA
                                                                                

HsCD00022178        ------------------------------------------------------------
XM_005249592.1      TTTTCAGGACTTCCAGCTTCCTTGGCAACAGGTCCTGTCAGAGCTCCAGTGGCCCCAGCC
NM_007342.2         TTTTCAGGACTTCCAGCTTCCTTGGCAACAGGTCCTGTCAGAGCTCCAGTGGCCCCAGCC
                                                                                

HsCD00022178        ------------------------------------------------------------
XM_005249592.1      TTTGGAGGTGGCAGTTCTGTGGCTGGTTTTGGTAGTCCGGGCTCACATTCTCACACTGCT
NM_007342.2         TTTGGAGGTGGCAGTTCTGTGGCTGGTTTTGGTAGTCCGGGCTCACATTCTCACACTGCT
                                                                                

HsCD00022178        ------------------------------------------------------------
XM_005249592.1      TTTTCTAAGCCATCCAGTGACACTTTTGGAAATAGCAGCATATCCACTTCTCTGTCAGCC
NM_007342.2         TTTTCTAAGCCATCCAGTGACACTTTTGGAAATAGCAGCATATCCACTTCTCTGTCAGCC
                                                                                

HsCD00022178        ------------------------------------------------------------
XM_005249592.1      TCAAGCAGCATCATTGCAACAGATAATGTGTTATTCACACCCAGAGATAAACTAACAGTA
NM_007342.2         TCAAGCAGCATCATTGCAACAGATAATGTGTTATTCACACCCAGAGATAAACTAACAGTA
                                                                                

HsCD00022178        ------------------------------------------------------------
XM_005249592.1      GAAGAACTGGAACAATTTCAATCCAAGAAATTTACTCTGGGAAAAATTCCATTAAAGCCT
NM_007342.2         GAAGAACTGGAACAATTTCAATCCAAGAAATTTACTCTGGGAAAAATTCCATTAAAGCCT
                                                                                

HsCD00022178        ---------------------------
XM_005249592.1      CCACCTCTGGAACTTCTAAATGTTTAA
NM_007342.2         CCACCTCTGGAACTTCTAAATGTTTAA
                                               

Pathways

Reactome : Antiviral mechanism by IFN-stimulated genes
Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Cytokine Signaling in Immune system
Reactome : Disease
Reactome : Export of Viral Ribonucleoproteins from Nucleus
Reactome : Gene Expression
Reactome : Glucose transport
Reactome : Glycogen storage diseases
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : Hexose transport
Reactome : Host Interactions of HIV factors
Reactome : ISG15 antiviral mechanism
Reactome : Immune System
Reactome : Influenza Infection
Reactome : Influenza Life Cycle
Reactome : Interactions of Rev with host cellular proteins
Reactome : Interactions of Vpr with host cellular proteins
Reactome : Interferon Signaling
Reactome : Late Phase of HIV Life Cycle
Reactome : M Phase
Reactome : Metabolism
Reactome : Metabolism of RNA
Reactome : Metabolism of carbohydrates
Reactome : Metabolism of non-coding RNA
Reactome : Mitotic M-M/G1 phases
Reactome : Mitotic Prophase
Reactome : Myoclonic epilepsy of Lafora
Reactome : NEP/NS2 Interacts with the Cellular Export Machinery
Reactome : Nuclear Envelope Breakdown
Reactome : Nuclear Pore Complex (NPC) Disassembly
Reactome : Nuclear import of Rev protein
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : Regulation of Glucokinase by Glucokinase Regulatory Protein
Reactome : Rev-mediated nuclear export of HIV RNA
Reactome : SLC-mediated transmembrane transport
Reactome : Transmembrane transport of small molecules
Reactome : Transport of Mature Transcript to Cytoplasm
Reactome : Transport of Mature mRNA Derived from an Intronless Transcript
Reactome : Transport of Mature mRNA derived from an Intron-Containing Transcript
Reactome : Transport of Mature mRNAs Derived from Intronless Transcripts
Reactome : Transport of Ribonucleoproteins into the Host Nucleus
Reactome : Transport of the SLBP Dependant Mature mRNA
Reactome : Transport of the SLBP independent Mature mRNA
Reactome : Vpr-mediated nuclear import of PICs
Reactome : snRNP Assembly

Protein

SMART domain : ZnF_C3H1 : zinc finger
UniProt : O15504
HPRD : 14855

Clone

Cloning Information : 23461
HIP Master Clone ID : 53837
Original Clone ID : FLH023461.01X

Organism


TAX_ID : 9606
Species Specific ID: 11097

Genome

Chromosome : 7
Map Location : 7p15
Ensembl : ENSG00000136243

Reagents

Labome : NUPL2-antibody