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ZNF256 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF256
Gene Name:  zinc finger protein 256
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH110397.01X
Keyword: potential short variant
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: Short variant or partial clone comparing to NM_005773
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00022228

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1425nts         Open reading frame : 1 to 1425
Reference Sequence Alignment

ATGTTTTTGAGCAGCTGCACATTTGAAGTATCTGGGAAGCCCTTCACTTGCAAGGAGGTT
GGGAAGGATTTCCTGGTGAGATCAAGATTTCTTCAGCAACAGGCTGCTCACACCAGAAAG
AAGTCAAACAGAACCAAGAGTGCAGTGGCCTTTCACAGTGTAAAAAATCATTACAACTGG
GGAGAATGTGTGAAAGCTTTCAGCTACAAACATGTACGTGTTCAGCACCAGGGAGACCTC
ATTAGGGAAAGATCTTACATGTGCAGTGAATGTGGGAAATCTTTTAGCACAAGCTGTAGC
CTCAGTGATCATTTGAGAGTTCACACTTCAGAAAAGCCTTATACATGTGGAGAATGTGGG
AAATCCTATAGGCAAAGCTCTAGCCTTATTACGCACCGAAGAATTCACACTGGAGTAAGA
CCTCATCAATGTGATGAATGTGGAAAATTATTTAACAGGAAGTATGACCTTCTTATACAT
CAGAGAGTTCATACTGGAGAAAGGCCTTACAAGTGCAGTGAATGTGGGAAATCCTTTAGC
CATAGCTCTAGCCTCATTACACACCAGAGAATTCATACTGGAATGAGGCCTTATGAGTGC
AGTGAATGTGGGAAATCTTTTATCCATAGTTCTAGCCTTATTACACACCAGAGAGTTCAC
ACTGGTACAAGGCCTTATATGTGCAGTGAATGTGGGAAATCCTTTAGCCAGAGCTGTCAC
CTCATTAAACACCGGAGACTTCACATTGGAGAAGGGCCTTATGAGTGTAGTGAATGTGGG
AAATTGTTTACTTATAGATCTCGTTTCTTCCAACACCAGAGAGTTCATACTGGAGTAAGA
TCTCATGAATGTCATGAATGTGGAAAATTATTTAGCAGGAAATTTGACCTCATTGTACAT
GAGAGAGTTCACACAGGAGAAAGGCCATATGAGTGCAGTGAATGTGGAAAATCCTTTACC
TGTAAATCCTACCTCATCTCACACTGGAAAGTTCATACTGGAGCAAGGCCTTATGAATGT
GGGGAGTGTGGGAAATCATTTACTCATAGCTCTACGCTCCTTCAACACCAGAGAGTTCAC
ACTGGAGAAAGGCCTTATGAGTGCAATGAATGTGGGAAGTTTTTTAGCCAGAGCTCCAGC
CTCATTAGACATAGGAGAAGTCACACCGGAGAAAGGCCTTATGAGTGCAGTGAGTGTTGG
AAATCCTTTAGTAACCACTCTAGCCTCGTTAAACACCGAAGAGTTCATACCGGAGAAAGG
CCTTATGAATGCAGTGAATGTGGAAAATCCTTTAGCCAGAGCTCTAACCTCACTAATCAC
CAGCGAATTCACAGTGGGGAAAGGCCTTATGAGTGTAGTGACTGTGGAAAATTTTTTACC
TTCAACTCCAACCTCCTAAAACATCAGAACGTTCACAAGGGATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2081
Start on reference sequence 198
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF256
Symbol Nomenclature : ZNF256
SYNONYM : BMZF-3; BMZF3
Designation : bone marrow zinc finger 3
Full Nomenclature : zinc finger protein 256
GENEID : 10172
HGNC : 13049
MIM : 606956
Vega : OTTHUMG00000183449
Target GenBank: BC001438

 

Reference Sequence Alignment

  

HsCD00022228        ------------------------------------------------------------
NM_005773.2         ATGGCGGCGGCCGAGCTGACGGCCCCGGCCCAGGGCATTGTGACCTTTGAGGACGTGGCT
XM_005258407.1      ------------------------------------------------------------
                                                                                

HsCD00022228        ------------------------------------------------------------
NM_005773.2         GTTTACTTCTCCTGGAAGGAATGGGGTCTTCTTGATGAGGCTCAGAAATGCCTGTACCAC
XM_005258407.1      ------------------------------------------------------------
                                                                                

HsCD00022228        ------------------------------------------------------------
NM_005773.2         GATGTGATGCTGGAGAACTTGACACTTACAACCTCCCTGGGTGGTTCTGGAGCAGGGGAT
XM_005258407.1      ------------------------------------------------------------
                                                                                

HsCD00022228        ------------------------------------------------------------
NM_005773.2         GAGGAGGCACCTTATCAGCAGAGCACTTCTCCACAGCGGGTGTCACAGGTTAGGATTCCT
XM_005258407.1      ------------------------------------------------------------
                                                                                

HsCD00022228        ------------------------------------------------------------
NM_005773.2         AAGGCCCTTCCTTCTCCCCAGAAGACCAACCCCTGTGAGATATGTGGCCCAGTCTTGAGA
XM_005258407.1      ------------------------------------------------------------
                                                                                

HsCD00022228        ------------------------------------------------------------
NM_005773.2         CAGATTTTGCACTTGGTTGAACACCAAGGAACACACCATGGTCAGAAACTGTATACAGAC
XM_005258407.1      ------------------------------------------------------------
                                                                                

HsCD00022228        ------------------------------------------------------------
NM_005773.2         GGGGCATGTAGGAAACAATTACAATTTACTGCATACCTTCATCAGCACCAGAAGCAGCAT
XM_005258407.1      ------------------------------------------------------------
                                                                                

HsCD00022228        ---------------------------------------ATGTTTTTGAGCAGCTGCACA
NM_005773.2         GTTGGACAGAAACACTTCAGAAGCAATGGGGGCAGAGACATGTTTTTGAGCAGCTGCACA
XM_005258407.1      ---------------------------------------ATGTTTTTGAGCAGCTGCACA
                                                           *********************

HsCD00022228        TTTGAAGTATCTGGGAAGCCCTTCACTTGCAAGGAGGTTGGGAAGGATTTCCTGGTGAGA
NM_005773.2         TTTGAAGTATCTGGGAAGCCCTTCACTTGCAAGGAGGTTGGGAAGGATTTCCTGGTGAGA
XM_005258407.1      TTTGAAGTATCTGGGAAGCCCTTCACTTGCAAGGAGGTTGGGAAGGATTTCCTGGTGAGA
                    ************************************************************

HsCD00022228        TCAAGATTTCTTCAGCAACAGGCTGCTCACACCAGAAAGAAGTCAAACAGAACCAAGAGT
NM_005773.2         TCAAGATTTCTTCAGCAACAGGCTGCTCACACCAGAAAGAAGTCAAACAGAACCAAGAGT
XM_005258407.1      TCAAGATTTCTTCAGCAACAGGCTGCTCACACCAGAAAGAAGTCAAACAGAACCAAGAGT
                    ************************************************************

HsCD00022228        GCAGTGGCCTTTCACAGTGTAAAAAATCATTACAACTGGGGAGAATGTGTGAAAGCTTTC
NM_005773.2         GCAGTGGCCTTTCACAGTGTAAAAAATCATTACAACTGGGGAGAATGTGTGAAAGCTTTC
XM_005258407.1      GCAGTGGCCTTTCACAGTGTAAAAAATCATTACAACTGGGGAGAATGTGTGAAAGCTTTC
                    ************************************************************

HsCD00022228        AGCTACAAACATGTACGTGTTCAGCACCAGGGAGACCTCATTAGGGAAAGATCTTACATG
NM_005773.2         AGCTACAAACATGTACGTGTTCAGCACCAGGGAGACCTCATTAGGGAAAGATCTTACATG
XM_005258407.1      AGCTACAAACATGTACGTGTTCAGCACCAGGGAGACCTCATTAGGGAAAGATCTTACATG
                    ************************************************************

HsCD00022228        TGCAGTGAATGTGGGAAATCTTTTAGCACAAGCTGTAGCCTCAGTGATCATTTGAGAGTT
NM_005773.2         TGCAGTGAATGTGGGAAATCTTTTAGCACAAGCTGTAGCCTCAGTGATCATTTGAGAGTT
XM_005258407.1      TGCAGTGAATGTGGGAAATCTTTTAGCACAAGCTGTAGCCTCAGTGATCATTTGAGAGTT
                    ************************************************************

HsCD00022228        CACACTTCAGAAAAGCCTTATACATGTGGAGAATGTGGGAAATCCTATAGGCAAAGCTCT
NM_005773.2         CACACTTCAGAAAAGCCTTATACATGTGGAGAATGTGGGAAATCCTATAGGCAAAGCTCT
XM_005258407.1      CACACTTCAGAAAAGCCTTATACATGTGGAGAATGTGGGAAATCCTATAGGCAAAGCTCT
                    ************************************************************

HsCD00022228        AGCCTTATTACGCACCGAAGAATTCACACTGGAGTAAGACCTCATCAATGTGATGAATGT
NM_005773.2         AGCCTTATTACGCACCGAAGAATTCACACTGGAGTAAGACCTCATCAATGTGATGAATGT
XM_005258407.1      AGCCTTATTACGCACCGAAGAATTCACACTGGAGTAAGACCTCATCAATGTGATGAATGT
                    ************************************************************

HsCD00022228        GGAAAATTATTTAACAGGAAGTATGACCTTCTTATACATCAGAGAGTTCATACTGGAGAA
NM_005773.2         GGAAAATTATTTAACAGGAAGTATGACCTTCTTATACATCAGAGAGTTCATACTGGAGAA
XM_005258407.1      GGAAAATTATTTAACAGGAAGTATGACCTTCTTATACATCAGAGAGTTCATACTGGAGAA
                    ************************************************************

HsCD00022228        AGGCCTTACAAGTGCAGTGAATGTGGGAAATCCTTTAGCCATAGCTCTAGCCTCATTACA
NM_005773.2         AGGCCTTACAAGTGCAGTGAATGTGGGAAATCCTTTAGCCATAGCTCTAGCCTCATTACA
XM_005258407.1      AGGCCTTACAAGTGCAGTGAATGTGGGAAATCCTTTAGCCATAGCTCTAGCCTCATTACA
                    ************************************************************

HsCD00022228        CACCAGAGAATTCATACTGGAATGAGGCCTTATGAGTGCAGTGAATGTGGGAAATCTTTT
NM_005773.2         CACCAGAGAATTCATACTGGAATGAGGCCTTATGAGTGCAGTGAATGTGGGAAATCTTTT
XM_005258407.1      CACCAGAGAATTCATACTGGAATGAGGCCTTATGAGTGCAGTGAATGTGGGAAATCTTTT
                    ************************************************************

HsCD00022228        ATCCATAGTTCTAGCCTTATTACACACCAGAGAGTTCACACTGGTACAAGGCCTTATATG
NM_005773.2         ATCCATAGTTCTAGCCTTATTACACACCAGAGAGTTCACACTGGTACAAGGCCTTATATG
XM_005258407.1      ATCCATAGTTCTAGCCTTATTACACACCAGAGAGTTCACACTGGTACAAGGCCTTATATG
                    ************************************************************

HsCD00022228        TGCAGTGAATGTGGGAAATCCTTTAGCCAGAGCTGTCACCTCATTAAACACCGGAGACTT
NM_005773.2         TGCAGTGAATGTGGGAAATCCTTTAGCCAGAGCTGTCACCTCATTAAACACCGGAGACTT
XM_005258407.1      TGCAGTGAATGTGGGAAATCCTTTAGCCAGAGCTGTCACCTCATTAAACACCGGAGACTT
                    ************************************************************

HsCD00022228        CACATTGGAGAAGGGCCTTATGAGTGTAGTGAATGTGGGAAATTGTTTACTTATAGATCT
NM_005773.2         CACATTGGAGAAGGGCCTTATGAGTGTAGTGAATGTGGGAAATTGTTTACTTATAGATCT
XM_005258407.1      CACATTGGAGAAGGGCCTTATGAGTGTAGTGAATGTGGGAAATTGTTTACTTATAGATCT
                    ************************************************************

HsCD00022228        CGTTTCTTCCAACACCAGAGAGTTCATACTGGAGTAAGATCTCATGAATGTCATGAATGT
NM_005773.2         CGTTTCTTCCAACACCAGAGAGTTCATACTGGAGTAAGATCTCATGAATGTCATGAATGT
XM_005258407.1      CGTTTCTTCCAACACCAGAGAGTTCATACTGGAGTAAGATCTCATGAATGTCATGAATGT
                    ************************************************************

HsCD00022228        GGAAAATTATTTAGCAGGAAATTTGACCTCATTGTACATGAGAGAGTTCACACAGGAGAA
NM_005773.2         GGAAAATTATTTAGCAGGAAATTTGACCTCATTGTACATGAGAGAGTTCACACAGGAGAA
XM_005258407.1      GGAAAATTATTTAGCAGGAAATTTGACCTCATTGTACATGAGAGAGTTCACACAGGAGAA
                    ************************************************************

HsCD00022228        AGGCCATATGAGTGCAGTGAATGTGGAAAATCCTTTACCTGTAAATCCTACCTCATCTCA
NM_005773.2         AGGCCATATGAGTGCAGTGAATGTGGAAAATCCTTTACCTGTAAATCCTACCTCATCTCA
XM_005258407.1      AGGCCATATGAGTGCAGTGAATGTGGAAAATCCTTTACCTGTAAATCCTACCTCATCTCA
                    ************************************************************

HsCD00022228        CACTGGAAAGTTCATACTGGAGCAAGGCCTTATGAATGTGGGGAGTGTGGGAAATCATTT
NM_005773.2         CACTGGAAAGTTCATACTGGAGCAAGGCCTTATGAATGTGGGGAGTGTGGGAAATCATTT
XM_005258407.1      CACTGGAAAGTTCATACTGGAGCAAGGCCTTATGAATGTGGGGAGTGTGGGAAATCATTT
                    ************************************************************

HsCD00022228        ACTCATAGCTCTACGCTCCTTCAACACCAGAGAGTTCACACTGGAGAAAGGCCTTATGAG
NM_005773.2         ACTCATAGCTCTACGCTCCTTCAACACCAGAGAGTTCACACTGGAGAAAGGCCTTATGAG
XM_005258407.1      ACTCATAGCTCTACGCTCCTTCAACACCAGAGAGTTCACACTGGAGAAAGGCCTTATGAG
                    ************************************************************

HsCD00022228        TGCAATGAATGTGGGAAGTTTTTTAGCCAGAGCTCCAGCCTCATTAGACATAGGAGAAGT
NM_005773.2         TGCAATGAATGTGGGAAGTTTTTTAGCCAGAGCTCCAGCCTCATTAGACATAGGAGAAGT
XM_005258407.1      TGCAATGAATGTGGGAAGTTTTTTAGCCAGAGCTCCAGCCTCATTAGACATAGGAGAAGT
                    ************************************************************

HsCD00022228        CACACCGGAGAAAGGCCTTATGAGTGCAGTGAGTGTTGGAAATCCTTTAGTAACCACTCT
NM_005773.2         CACACCGGAGAAAGGCCTTATGAGTGCAGTGAGTGTTGGAAATCCTTTAGTAACCACTCT
XM_005258407.1      CACACCGGAGAAAGGCCTTATGAGTGCAGTGAGTGTTGGAAATCCTTTAGTAACCACTCT
                    ************************************************************

HsCD00022228        AGCCTCGTTAAACACCGAAGAGTTCATACCGGAGAAAGGCCTTATGAATGCAGTGAATGT
NM_005773.2         AGCCTCGTTAAACACCGAAGAGTTCATACCGGAGAAAGGCCTTATGAATGCAGTGAATGT
XM_005258407.1      AGCCTCGTTAAACACCGAAGAGTTCATACCGGAGAAAGGCCTTATGAATGCAGTGAATGT
                    ************************************************************

HsCD00022228        GGAAAATCCTTTAGCCAGAGCTCTAACCTCACTAATCACCAGCGAATTCACAGTGGGGAA
NM_005773.2         GGAAAATCCTTTAGCCAGAGCTCTAACCTCACTAATCACCAGCGAATTCACAGTGGGGAA
XM_005258407.1      GGAAAATCCTTTAGCCAGAGCTCTAACCTCACTAATCACCAGCGAATTCACAGTGGGGAA
                    ************************************************************

HsCD00022228        AGGCCTTATGAGTGTAGTGACTGTGGAAAATTTTTTACCTTCAACTCCAACCTCCTAAAA
NM_005773.2         AGGCCTTATGAGTGTAGTGACTGTGGAAAATTTTTTACCTTCAACTCCAACCTCCTAAAA
XM_005258407.1      AGGCCTTATGAGTGTAGTGACTGTGGAAAATTTTTTACCTTCAACTCCAACCTCCTAAAA
                    ************************************************************

HsCD00022228        CATCAGAACGTTCACAAGGGATAG
NM_005773.2         CATCAGAACGTTCACAAGGGATAA
XM_005258407.1      CATCAGAACGTTCACAAGGGATAA
                    ***********************.

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q9Y2P7
HPRD : 06095

Clone

Cloning Information : 110397
HIP Master Clone ID : 86687
Original Clone ID : FLH110397.01X

Organism


TAX_ID : 9606
Species Specific ID: 10172

Genome

Chromosome : 19
Map Location : 19q13.43
Ensembl : ENSG00000152454

Reagents

Labome : ZNF256-antibody