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STAR (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  STAR
Gene Name:  steroidogenic acute regulatory protein
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH024141.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00002235

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 858nts         Open reading frame : 1 to 858
Reference Sequence Alignment

ATGCTGCTAGCGACATTCAAGCTGTGCGCTGGGAGCTCCTACAGACACATGCGCAACATG
AAGGGGCTGAGGCAACAGGCTGTGATGGCCATCAGCCAGGAGCTGAACCGGAGGGCCCTG
GGGGGCCCCACCCCTAGCACGTGGATTAACCAGGTTCGGCGGCGGAGCTCTCTACTCGGT
TCTCGGCTGGAAGAGACTCTCTACAGTGACCAGGAGCTGGCCTATCTCCAGCAGGGGGAG
GAGGCCATGCAGAAGGCCTTGGGCATCCTTAGCAACCAAGAGGGCTGGAAGAAGGAGAGT
CAGCAGGACAATGGGGACAAAGTGATGAGTAAAGTGGTCCCAGATGTGGGCAAGGTGTTC
CGGCTGGAGGTCGTGGTGGACCAGCCCATGGAGAGGCTCTATGAAGAGCTCGTGGAGCGC
ATGGAAGCAATGGGGGAGTGGAACCCCAATGTCAAGGAGATCAAGGTCCTGCAGAAGATC
GGAAAAGATACATTCATTACTCACGAGCTGGCTGCCGAGGCAGCAGGAAACCTGGTGGGG
CCCCGTGACTTTGTGAGCGTGCGCTGTGCCAAGCGCCGAGGCTCCACCTGTGTGCTGGCT
GGCATGGCCACAGACTTCGGGAACATGCCTGAGCAGAAGGGTGTCATCAGGGCGGAGCAC
GGTCCCACTTGCATGGTGCTTCACCCGTTGGCTGGAAGTCCCTCTAAGACCAAACTTACG
TGGCTACTCAGCATCGACCTCAAGGGGTGGCTGCCCAAGAGCATCATCAACCAGGTCCTG
TCCCAGACCCAGGTGGATTTTGCCAACCACCTGCGCAAGCGCCTGGAGTCCCACCCTGCC
TCTGAAGCCAGGTGTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 923
Start on reference sequence 66
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : STAR
Symbol Nomenclature : STAR
SYNONYM : STARD1
Designation : START domain containing 1|START domain-containing protein 1|StAR-related lipid transfer (START) domain containing 1|cholesterol trafficker|mitochondrial steroid acute regulatory protein|steroid acute regulatory protein|steroidogenic acute regulator|steroidogenic acute regulatory protein, mitochondrial
Full Nomenclature : steroidogenic acute regulatory protein
GENEID : 6770
GI : 61370504
GenBank Accession : AY891579
HGNC : 11359
MIM : 600617
Target GenBank: BC010550

 

Reference Sequence Alignment

  

HsCD00002235      ATGCTGCTAGCGACATTCAAGCTGTGCGCTGGGAGCTCCTACAGACACATGCGCAACATG
NM_000349.2       ATGCTGCTAGCGACATTCAAGCTGTGCGCTGGGAGCTCCTACAGACACATGCGCAACATG
                  ************************************************************

HsCD00002235      AAGGGGCTGAGGCAACAGGCTGTGATGGCCATCAGCCAGGAGCTGAACCGGAGGGCCCTG
NM_000349.2       AAGGGGCTGAGGCAACAGGCTGTGATGGCCATCAGCCAGGAGCTGAACCGGAGGGCCCTG
                  ************************************************************

HsCD00002235      GGGGGCCCCACCCCTAGCACGTGGATTAACCAGGTTCGGCGGCGGAGCTCTCTACTCGGT
NM_000349.2       GGGGGCCCCACCCCTAGCACGTGGATTAACCAGGTTCGGCGGCGGAGCTCTCTACTCGGT
                  ************************************************************

HsCD00002235      TCTCGGCTGGAAGAGACTCTCTACAGTGACCAGGAGCTGGCCTATCTCCAGCAGGGGGAG
NM_000349.2       TCTCGGCTGGAAGAGACTCTCTACAGTGACCAGGAGCTGGCCTATCTCCAGCAGGGGGAG
                  ************************************************************

HsCD00002235      GAGGCCATGCAGAAGGCCTTGGGCATCCTTAGCAACCAAGAGGGCTGGAAGAAGGAGAGT
NM_000349.2       GAGGCCATGCAGAAGGCCTTGGGCATCCTTAGCAACCAAGAGGGCTGGAAGAAGGAGAGT
                  ************************************************************

HsCD00002235      CAGCAGGACAATGGGGACAAAGTGATGAGTAAAGTGGTCCCAGATGTGGGCAAGGTGTTC
NM_000349.2       CAGCAGGACAATGGGGACAAAGTGATGAGTAAAGTGGTCCCAGATGTGGGCAAGGTGTTC
                  ************************************************************

HsCD00002235      CGGCTGGAGGTCGTGGTGGACCAGCCCATGGAGAGGCTCTATGAAGAGCTCGTGGAGCGC
NM_000349.2       CGGCTGGAGGTCGTGGTGGACCAGCCCATGGAGAGGCTCTATGAAGAGCTCGTGGAGCGC
                  ************************************************************

HsCD00002235      ATGGAAGCAATGGGGGAGTGGAACCCCAATGTCAAGGAGATCAAGGTCCTGCAGAAGATC
NM_000349.2       ATGGAAGCAATGGGGGAGTGGAACCCCAATGTCAAGGAGATCAAGGTCCTGCAGAAGATC
                  ************************************************************

HsCD00002235      GGAAAAGATACATTCATTACTCACGAGCTGGCTGCCGAGGCAGCAGGAAACCTGGTGGGG
NM_000349.2       GGAAAAGATACATTCATTACTCACGAGCTGGCTGCCGAGGCAGCAGGAAACCTGGTGGGG
                  ************************************************************

HsCD00002235      CCCCGTGACTTTGTGAGCGTGCGCTGTGCCAAGCGCCGAGGCTCCACCTGTGTGCTGGCT
NM_000349.2       CCCCGTGACTTTGTGAGCGTGCGCTGTGCCAAGCGCCGAGGCTCCACCTGTGTGCTGGCT
                  ************************************************************

HsCD00002235      GGCATGGCCACAGACTTCGGGAACATGCCTGAGCAGAAGGGTGTCATCAGGGCGGAGCAC
NM_000349.2       GGCATGGCCACAGACTTCGGGAACATGCCTGAGCAGAAGGGTGTCATCAGGGCGGAGCAC
                  ************************************************************

HsCD00002235      GGTCCCACTTGCATGGTGCTTCACCCGTTGGCTGGAAGTCCCTCTAAGACCAAACTTACG
NM_000349.2       GGTCCCACTTGCATGGTGCTTCACCCGTTGGCTGGAAGTCCCTCTAAGACCAAACTTACG
                  ************************************************************

HsCD00002235      TGGCTACTCAGCATCGACCTCAAGGGGTGGCTGCCCAAGAGCATCATCAACCAGGTCCTG
NM_000349.2       TGGCTACTCAGCATCGACCTCAAGGGGTGGCTGCCCAAGAGCATCATCAACCAGGTCCTG
                  ************************************************************

HsCD00002235      TCCCAGACCCAGGTGGATTTTGCCAACCACCTGCGCAAGCGCCTGGAGTCCCACCCTGCC
NM_000349.2       TCCCAGACCCAGGTGGATTTTGCCAACCACCTGCGCAAGCGCCTGGAGTCCCACCCTGCC
                  ************************************************************

HsCD00002235      TCTGAAGCCAGGTGTTTG
NM_000349.2       TCTGAAGCCAGGTGTTGA
                  **************** .

Protein

SMART domain : START : in StAR and phosphatidylcholine transfer protein
UniProt : P25092
HPRD : 02794

Clone

Cloning Information : 24141
HIP Master Clone ID : 54517
Original Clone ID : FLH024141.01L

Organism


TAX_ID : 9606
Species Specific ID: 2984

Genome

Chromosome : 8
Map Location : 8p11.2