DNASU Plasmid Repository • 480.965.5697 | Email

IRAK2 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  IRAK2
Gene Name:  interleukin-1 receptor-associated kinase 2
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH172891.01F
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors: HIP
HsCD00022389

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1773nts         Open reading frame : 1 to 1773
Reference Sequence Alignment

ATGGCCTGCTACATCTACCAGCTGCCCTCCTGGGTGCTGGACGACCTGTGCCGCAACATG
GACGCGCTCAGCGAGTGGGACTGGATGGAGTTCGCCTCCTACGTGATCACAGACCTGACC
CAGCTGCGGAAGATCAAGTCCATGGAGCGGGTGCAGGGTGTGAGCATCACGCGGGAGCTG
CTGTGGTGGTGGGGCATGCGGCAGGCCACCGTCCAGCAACTTGTGGACCTCCTGTGCCGC
CTGGAGCTCTACCGGGCTGCCCAGATCATCCTGAACTGGAAACCGGCTCCTGAAATCAGG
TGTCCCATTCCAGCCTTCCCTGACTCTGTGAAGCCAGAAAAGCCTTTGGCAGCTTCTGTA
AGAAAGGCTGAGGATGAACAGGAAGAGGGGCAGCCTGTGAGGATGGCCACCTTTCCAGGC
CCAGGGTCCTCTCCAGCCAGAGCCCACCAGCCGGCCTTTCTCCAGCCTCCTGAAGAAGAT
GCCCCTCATTCGTTGAGAAGCGACCTCCCCACTTCGTCTGATTCAAAGGACTTCAGCACC
TCCATTCCTAAGCAGGAAAAACTTTTGAGCTTGGCTGGAGACAGCCTTTTCTGGAGTGAG
GCAGACGTGGTCCAGGCAACCGATGACTTCAATCAAAACCGCAAAATCAGCCAGGGGACC
TTTGCTGACGTCTACAGAGGGCACAGGCACGGGAAGCCATTCGTCTTCAAGAAGCTCAGA
GAGACAGCCTGTTCAAGTCCAGGATCAATCGAAAGATTCTTCCAGGCAGAGTTGCAGATT
TGTCTTAGATGCTGCCACCCCAATGTCTTACCTGTGCTGGGCTTCTGTGCTGCAAGACAG
TTTCACAGCTTCATCTACCCCTACATGGCAAATGGTTCCCTACAGGACAGACTGCAGGGT
CAGGGTGGCTCGGACCCCCTCCCCTGGCCCCAGCGTGTCAGCATCTGCTCAGGGCTGCTC
TGTGCCGTCGAGTACCTGCATGGTCTGGAGATCATCCACAGCAACGTCAAGAGCTCTAAT
GTCTTGCTGGACCAAAATCTCACCCCCAAACTTGCTCACCCAATGGCTCATCTGTGTCCT
GTCAACAAAAGGTCAAAATACACCATGATGAAGACTCACCTGCTCCGGACGTCAGCCGCG
TATCTGCCAGAGGATTTCATCCGGGTGGGGCAGCTGACAAAGCGAGTGGACATCTTCAGC
TGTGGAATAGTGTTGGCCGAGGTCCTCACGGGCATCCCTGCAATGGATAACAACCGAAGC
CCGGTTTACCTGAAGGACTTACTCCTCAGTGAAATTCCAAGCAGCACCGCCTCGCTCTGC
TCCAGGAAGACGGGCGTGGAGAACGTGATGGCAAAGGAGATCTGCCAGAAGTACCTGGAG
AAGGGCGCAGGGAGGCTTCCGGAGGACTGCGCCGAGGCCCTGGCCACGGCTGCCTGCCTG
TGCCTGCGGAGGCGTAACACCAGCCTGCAGGAGGTGTGTGGCTCTGTGGCTGCTGTGGAA
GAGCGGCTCCGAGGTCGGGAGACGTTGCTCCCTTGGAGTGGGCTTTCTGAGGGTACAGGC
TCTTCTTCCAACACCCCAGAGGAAACAGACGACGTTGACAATTCCAGCCTTGATGCCTCC
TCCTCCATGAGTGTGGCACCCTGGGCAGGGGCTGCCACCCCACTTCTCCCCACAGAGAAT
GGGGAAGGAAGGCTGCGGGTCATCGTGGGAAGGGAGGCTGACTCCTCCTCTGAGGCCTGT
GTTGGCCTGGAGCCTCCCCAGGATGTTACATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1782
Start on reference sequence 10
Mutations:
None
Discrepancy:
c492g; t1293a,D431E
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : IRAK2
Symbol Nomenclature : IRAK2
SYNONYM : IRAK-2
Designation : interleukin-1 receptor associated kinase-2|interleukin-1 receptor-associated kinase-like 2
Full Nomenclature : interleukin-1 receptor-associated kinase 2
GENEID : 3656
HGNC : 6113
MIM : 603304
Vega : OTTHUMG00000155358
Target GenBank: NM_001570

 

Reference Sequence Alignment

  

HsCD00022389      ATGGCCTGCTACATCTACCAGCTGCCCTCCTGGGTGCTGGACGACCTGTGCCGCAACATG
NM_001570.3       ATGGCCTGCTACATCTACCAGCTGCCCTCCTGGGTGCTGGACGACCTGTGCCGCAACATG
                  ************************************************************

HsCD00022389      GACGCGCTCAGCGAGTGGGACTGGATGGAGTTCGCCTCCTACGTGATCACAGACCTGACC
NM_001570.3       GACGCGCTCAGCGAGTGGGACTGGATGGAGTTCGCCTCCTACGTGATCACAGACCTGACC
                  ************************************************************

HsCD00022389      CAGCTGCGGAAGATCAAGTCCATGGAGCGGGTGCAGGGTGTGAGCATCACGCGGGAGCTG
NM_001570.3       CAGCTGCGGAAGATCAAGTCCATGGAGCGGGTGCAGGGTGTGAGCATCACGCGGGAGCTG
                  ************************************************************

HsCD00022389      CTGTGGTGGTGGGGCATGCGGCAGGCCACCGTCCAGCAACTTGTGGACCTCCTGTGCCGC
NM_001570.3       CTGTGGTGGTGGGGCATGCGGCAGGCCACCGTCCAGCAACTTGTGGACCTCCTGTGCCGC
                  ************************************************************

HsCD00022389      CTGGAGCTCTACCGGGCTGCCCAGATCATCCTGAACTGGAAACCGGCTCCTGAAATCAGG
NM_001570.3       CTGGAGCTCTACCGGGCTGCCCAGATCATCCTGAACTGGAAACCGGCTCCTGAAATCAGG
                  ************************************************************

HsCD00022389      TGTCCCATTCCAGCCTTCCCTGACTCTGTGAAGCCAGAAAAGCCTTTGGCAGCTTCTGTA
NM_001570.3       TGTCCCATTCCAGCCTTCCCTGACTCTGTGAAGCCAGAAAAGCCTTTGGCAGCTTCTGTA
                  ************************************************************

HsCD00022389      AGAAAGGCTGAGGATGAACAGGAAGAGGGGCAGCCTGTGAGGATGGCCACCTTTCCAGGC
NM_001570.3       AGAAAGGCTGAGGATGAACAGGAAGAGGGGCAGCCTGTGAGGATGGCCACCTTTCCAGGC
                  ************************************************************

HsCD00022389      CCAGGGTCCTCTCCAGCCAGAGCCCACCAGCCGGCCTTTCTCCAGCCTCCTGAAGAAGAT
NM_001570.3       CCAGGGTCCTCTCCAGCCAGAGCCCACCAGCCGGCCTTTCTCCAGCCTCCTGAAGAAGAT
                  ************************************************************

HsCD00022389      GCCCCTCATTCGTTGAGAAGCGACCTCCCCACTTCGTCTGATTCAAAGGACTTCAGCACC
NM_001570.3       GCCCCTCATTCCTTGAGAAGCGACCTCCCCACTTCGTCTGATTCAAAGGACTTCAGCACC
                  *********** ************************************************

HsCD00022389      TCCATTCCTAAGCAGGAAAAACTTTTGAGCTTGGCTGGAGACAGCCTTTTCTGGAGTGAG
NM_001570.3       TCCATTCCTAAGCAGGAAAAACTTTTGAGCTTGGCTGGAGACAGCCTTTTCTGGAGTGAG
                  ************************************************************

HsCD00022389      GCAGACGTGGTCCAGGCAACCGATGACTTCAATCAAAACCGCAAAATCAGCCAGGGGACC
NM_001570.3       GCAGACGTGGTCCAGGCAACCGATGACTTCAATCAAAACCGCAAAATCAGCCAGGGGACC
                  ************************************************************

HsCD00022389      TTTGCTGACGTCTACAGAGGGCACAGGCACGGGAAGCCATTCGTCTTCAAGAAGCTCAGA
NM_001570.3       TTTGCTGACGTCTACAGAGGGCACAGGCACGGGAAGCCATTCGTCTTCAAGAAGCTCAGA
                  ************************************************************

HsCD00022389      GAGACAGCCTGTTCAAGTCCAGGATCAATCGAAAGATTCTTCCAGGCAGAGTTGCAGATT
NM_001570.3       GAGACAGCCTGTTCAAGTCCAGGATCAATCGAAAGATTCTTCCAGGCAGAGTTGCAGATT
                  ************************************************************

HsCD00022389      TGTCTTAGATGCTGCCACCCCAATGTCTTACCTGTGCTGGGCTTCTGTGCTGCAAGACAG
NM_001570.3       TGTCTTAGATGCTGCCACCCCAATGTCTTACCTGTGCTGGGCTTCTGTGCTGCAAGACAG
                  ************************************************************

HsCD00022389      TTTCACAGCTTCATCTACCCCTACATGGCAAATGGTTCCCTACAGGACAGACTGCAGGGT
NM_001570.3       TTTCACAGCTTCATCTACCCCTACATGGCAAATGGTTCCCTACAGGACAGACTGCAGGGT
                  ************************************************************

HsCD00022389      CAGGGTGGCTCGGACCCCCTCCCCTGGCCCCAGCGTGTCAGCATCTGCTCAGGGCTGCTC
NM_001570.3       CAGGGTGGCTCGGACCCCCTCCCCTGGCCCCAGCGTGTCAGCATCTGCTCAGGGCTGCTC
                  ************************************************************

HsCD00022389      TGTGCCGTCGAGTACCTGCATGGTCTGGAGATCATCCACAGCAACGTCAAGAGCTCTAAT
NM_001570.3       TGTGCCGTCGAGTACCTGCATGGTCTGGAGATCATCCACAGCAACGTCAAGAGCTCTAAT
                  ************************************************************

HsCD00022389      GTCTTGCTGGACCAAAATCTCACCCCCAAACTTGCTCACCCAATGGCTCATCTGTGTCCT
NM_001570.3       GTCTTGCTGGACCAAAATCTCACCCCCAAACTTGCTCACCCAATGGCTCATCTGTGTCCT
                  ************************************************************

HsCD00022389      GTCAACAAAAGGTCAAAATACACCATGATGAAGACTCACCTGCTCCGGACGTCAGCCGCG
NM_001570.3       GTCAACAAAAGGTCAAAATACACCATGATGAAGACTCACCTGCTCCGGACGTCAGCCGCG
                  ************************************************************

HsCD00022389      TATCTGCCAGAGGATTTCATCCGGGTGGGGCAGCTGACAAAGCGAGTGGACATCTTCAGC
NM_001570.3       TATCTGCCAGAGGATTTCATCCGGGTGGGGCAGCTGACAAAGCGAGTGGACATCTTCAGC
                  ************************************************************

HsCD00022389      TGTGGAATAGTGTTGGCCGAGGTCCTCACGGGCATCCCTGCAATGGATAACAACCGAAGC
NM_001570.3       TGTGGAATAGTGTTGGCCGAGGTCCTCACGGGCATCCCTGCAATGGATAACAACCGAAGC
                  ************************************************************

HsCD00022389      CCGGTTTACCTGAAGGACTTACTCCTCAGTGAAATTCCAAGCAGCACCGCCTCGCTCTGC
NM_001570.3       CCGGTTTACCTGAAGGACTTACTCCTCAGTGATATTCCAAGCAGCACCGCCTCGCTCTGC
                  ********************************:***************************

HsCD00022389      TCCAGGAAGACGGGCGTGGAGAACGTGATGGCAAAGGAGATCTGCCAGAAGTACCTGGAG
NM_001570.3       TCCAGGAAGACGGGCGTGGAGAACGTGATGGCAAAGGAGATCTGCCAGAAGTACCTGGAG
                  ************************************************************

HsCD00022389      AAGGGCGCAGGGAGGCTTCCGGAGGACTGCGCCGAGGCCCTGGCCACGGCTGCCTGCCTG
NM_001570.3       AAGGGCGCAGGGAGGCTTCCGGAGGACTGCGCCGAGGCCCTGGCCACGGCTGCCTGCCTG
                  ************************************************************

HsCD00022389      TGCCTGCGGAGGCGTAACACCAGCCTGCAGGAGGTGTGTGGCTCTGTGGCTGCTGTGGAA
NM_001570.3       TGCCTGCGGAGGCGTAACACCAGCCTGCAGGAGGTGTGTGGCTCTGTGGCTGCTGTGGAA
                  ************************************************************

HsCD00022389      GAGCGGCTCCGAGGTCGGGAGACGTTGCTCCCTTGGAGTGGGCTTTCTGAGGGTACAGGC
NM_001570.3       GAGCGGCTCCGAGGTCGGGAGACGTTGCTCCCTTGGAGTGGGCTTTCTGAGGGTACAGGC
                  ************************************************************

HsCD00022389      TCTTCTTCCAACACCCCAGAGGAAACAGACGACGTTGACAATTCCAGCCTTGATGCCTCC
NM_001570.3       TCTTCTTCCAACACCCCAGAGGAAACAGACGACGTTGACAATTCCAGCCTTGATGCCTCC
                  ************************************************************

HsCD00022389      TCCTCCATGAGTGTGGCACCCTGGGCAGGGGCTGCCACCCCACTTCTCCCCACAGAGAAT
NM_001570.3       TCCTCCATGAGTGTGGCACCCTGGGCAGGGGCTGCCACCCCACTTCTCCCCACAGAGAAT
                  ************************************************************

HsCD00022389      GGGGAAGGAAGGCTGCGGGTCATCGTGGGAAGGGAGGCTGACTCCTCCTCTGAGGCCTGT
NM_001570.3       GGGGAAGGAAGGCTGCGGGTCATCGTGGGAAGGGAGGCTGACTCCTCCTCTGAGGCCTGT
                  ************************************************************

HsCD00022389      GTTGGCCTGGAGCCTCCCCAGGATGTTACATTG---------------------------
NM_001570.3       GTTGGCCTGGAGCCTCCCCAGGATGTTACAGAAACTTCGTGGCAAATTGAGATCAATGAG
                  ****************************** :.                           

HsCD00022389      ------------------------------------------------------------
NM_001570.3       GCCAAAAGGAAACTGATGGAGAATATTCTGCTCTACAAAGAGGAAAAAGTGGACAGCATT
                                                                              

HsCD00022389      ------------------
NM_001570.3       GAGCTCTTTGGCCCCTGA
                                    

Pathways

NCI : Endogenous TLR signaling
Reactome : Activated TLR4 signalling
Reactome : Cytokine Signaling in Immune system
Reactome : IRAK2 mediated activation of TAK1 complex
Reactome : IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Interleukin-1 signaling
Reactome : JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1
Reactome : MAP kinase activation in TLR cascade
Reactome : MyD88 cascade initiated on plasma membrane
Reactome : MyD88 dependent cascade initiated on endosome
Reactome : MyD88-independent cascade
Reactome : MyD88:Mal cascade initiated on plasma membrane
Reactome : NOD1/2 Signaling Pathway
Reactome : Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways
Reactome : Signaling by Interleukins
Reactome : TAK1 activates NFkB by phosphorylation and activation of IKKs complex
Reactome : TRAF6 Mediated Induction of proinflammatory cytokines
Reactome : TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
Reactome : TRIF-mediated TLR3/TLR4 signaling
Reactome : Toll Like Receptor 10 (TLR10) Cascade
Reactome : Toll Like Receptor 2 (TLR2) Cascade
Reactome : Toll Like Receptor 3 (TLR3) Cascade
Reactome : Toll Like Receptor 4 (TLR4) Cascade
Reactome : Toll Like Receptor 5 (TLR5) Cascade
Reactome : Toll Like Receptor 7/8 (TLR7/8) Cascade
Reactome : Toll Like Receptor 9 (TLR9) Cascade
Reactome : Toll Like Receptor TLR1:TLR2 Cascade
Reactome : Toll Like Receptor TLR6:TLR2 Cascade
Reactome : Toll-Like Receptors Cascades
Reactome : activated TAK1 mediates p38 MAPK activation
WikiPathway : IL-1 signaling pathway
WikiPathway : Regulation of toll-like receptor signaling pathway

Clone

Cloning Information : 172891
HIP Master Clone ID : 148036
Original Clone ID : FLH172891.01F

Organism


TAX_ID : 9606
Species Specific ID: 3656

Genome

Chromosome : 3
Map Location : 3p25.3
Ensembl : ENSG00000134070

Reagents

Labome : IRAK2-antibody