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PPP1R7 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  PPP1R7
Gene Name:  protein phosphatase 1, regulatory subunit 7
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH024632.01L
Keyword: potential short variant
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: Short variant or partial clone comparing to NM_002712
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00022452

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 843nts         Open reading frame : 1 to 843
Reference Sequence Alignment

ATGGCGGCGGAACGCGGCGCGGGGCAGCAACAGTCGCAGGAGATGATGGAGGTTGACAGG
CGGGTCGAGTCTGAAGAATCCGGCGATGAAGAAGGGAAGAAACACAGCAGTGGCATCGTG
GCCGACCTCAGTGAACAGAGCCTGAAGGATGGGGAGGAGCGGGGGGAGGAGGACCCAGAA
GAAGAACATGAGCTGCCTGTGGACATGGAAACCATCAACCTGGACAGAGATGCAGAGGAT
GTTGATTTGAATCACTATCGCATAGGGAAGATTGAAGGATTTGAGGTACTGAAGAAAGTG
AAGACTCTCTGCCTCCGCCAAAATTTAATTAAATGCATTGAGAATCTGGAGGAGCTACAG
AGTCTTCGAGAGCTGGATCTTTACGACAACCAGATCAAGAAGATTGAGAATCTGGAGGCG
CTAACAGAGCTGGAGATTCTAGATATTTCTTTTAATCTGCTGAGAAACATCGAAGGGGTT
GACAAGTTGACACGACTGAAAAAACTCTTCTTGGTCAACAATAAAATCAGTAAAATTGAG
AACTTAAGCAACTTACATCAACTACAGATGCTAGAGCTGGGATCTAACCGCATCCGGGCA
ATCGAAAATATCGACACCTTAACCAACCTGGAGAGTTTGTTTTTGGGGAAAAACAAAATT
ACTAAACTTCAGAACCTGGATGCGCTCACCAACCTGACAGTCCTCAGTATGCAGAGCAAC
CGGCTGACCAAGATCGAGGGTCTGCAGAACCTGGTGAACCTGCGGGAGCTGTACCTTAGC
CACAATGGCATCGAGGTCATCGAGGGCCTGGAGAACAATGTGCAGGACAGCCTCACGTAC
TAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 868
Start on reference sequence 26
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PPP1R7
Symbol Nomenclature : PPP1R7
SYNONYM : SDS22
Designation : protein phosphatase 1 regulatory subunit 22|protein phosphatase 1 regulatory subunit 7|protein phosphatase 1, regulatory (inhibitor) subunit 7
Full Nomenclature : protein phosphatase 1, regulatory subunit 7
GENEID : 5510
HGNC : 9295
MIM : 602877
Vega : OTTHUMG00000133390
Target GenBank: BC013001

 

Reference Sequence Alignment

  

HsCD00022452        ATGGCGGCGGAACGCGGCGCGGGGCAGCAACAGTCGCAGGAGATGATGGAGGTTGACAGG
NM_002712.2         ATGGCGGCGGAACGCGGCGCGGGGCAGCAACAGTCGCAGGAGATGATGGAGGTTGACAGG
NM_001282412.1      ------------------------------------------------ATGGTTGACAGG
NM_001282410.1      ATGGCGGCGGAACGCGGCGCGGGGCAGCAACAGTCGCAGGAGATGATGGAGGTTGACAGG
NM_001282413.1      ------------------------------------------------------------
                                                                                

HsCD00022452        CGGGTCGAGTCTGAAGAATCCGGCGATGAAGAAGGGAAGAAACACAGCAGTGGCATCGTG
NM_002712.2         CGGGTCGAGTCTGAAGAATCCGGCGATGAAGAAGGGAAGAAACACAGCAGTGGCATCGTG
NM_001282412.1      CGGGTCGAGTCTGAAGAATCCGGCGATGAAGAAGGGAAGAAACACAGCAGTGGCATCGTG
NM_001282410.1      CGGGTCGAGTCTGAAGAATCCGGCGATGAAGAAGGGAAGAAACACAGCAGTGGCATCGTG
NM_001282413.1      ------------------------------------------------------------
                                                                                

HsCD00022452        GCCGACCTCAGTGAACAGAGCCTGAAGGATGGGGAGGAGCGGGGGGAGGAGGACCCAGAA
NM_002712.2         GCCGACCTCAGTGAACAGAGCCTGAAGGATGGGGAGGAGCGGGGGGAGGAGGACCCAGAA
NM_001282412.1      GCCGACCTCAGTGAACAGAGCCTGAAGGATGGGGAGGAGCGGGGGGAGGAGGACCCAGAA
NM_001282410.1      GCCGACCTCAGTGAACAGAGCCTGAAGGATGGGGAGGAGCGGGGGGAGGAGGACCCAGAA
NM_001282413.1      ---------------------------------------------------------ATG
                                                                             .:.

HsCD00022452        GAAGAACATGAGCTGCCTGTGGACATGGAAACCATCAACCTGGACAGAGATGCAGAGGAT
NM_002712.2         GAAGAACATGAGCTGCCTGTGGACATGGAAACCATCAACCTGGACAGAGATGCAGAGGAT
NM_001282412.1      GAAGAACATGAGCTGCCTGTGGACATGGAAACCATCAACCTGGACAGAGATGCAGAGGAT
NM_001282410.1      GAAGAACATGAGCTGCCTGTGGACATGGAAACCATCAACCTGGACAGAGATGCAGAGGAT
NM_001282413.1      GAAGAACATGAGCTGCCTGTGGACATGGAAACCATCAACCTGGACAGAGATGCAGAGGAT
                    ************************************************************

HsCD00022452        GTTGATTTGAATCACTATCGCATAGGGAAGATTGAAGGATTTGAGGTACTGAAGAAAGTG
NM_002712.2         GTTGATTTGAATCACTATCGCATAGGGAAGATTGAAGGATTTGAGGTACTGAAGAAAGTG
NM_001282412.1      GTTGATTTGAATCACTATCGCATAGGGAAGATTGAAGGATTTGAGGTACTGAAGAAAGTG
NM_001282410.1      GTTGATTTGAATCACTATCGCATAGGGAAGATTGAAGGATTTGAGGTACTGAAGAAAGTG
NM_001282413.1      GTTGATTTGAATCACTATCGCATAGGGAAGATTGAAGGATTTGAGGTACTGAAGAAAGTG
                    ************************************************************

HsCD00022452        AAGACTCTCTGCCTCCGCCAAAATTTAATTAAATGCATTGAGAATCTGGAGGAGCTACAG
NM_002712.2         AAGACTCTCTGCCTCCGCCAAAATTTAATTAAATGCATTGAGAATCTGGAGGAGCTACAG
NM_001282412.1      AAGACTCTCTGCCTCCGCCAAAATTTAATTAAATGCATTGAGAATCTGGAGGAGCTACAG
NM_001282410.1      AAGACTCTCTGCCTCCGCCAAAATTTAATTAAATGCATTGAGAATCTGGAGGAGCTACAG
NM_001282413.1      AAGACTCTCTGCCTCCGCCAAAATTTAATTAAATGCATTGAGAATCTGGAGGAGCTACAG
                    ************************************************************

HsCD00022452        AGTCTTCGAGAGCTGGATCTTTACGACAACCAGATCAAGAAGATTGAGAATCTGGAGGCG
NM_002712.2         AGTCTTCGAGAGCTGGATCTTTACGACAACCAGATCAAGAAGATTGAGAATCTGGAGGCG
NM_001282412.1      AGTCTTCGAGAGCTGGATCTTTACGACAACCAGATCAAGAAGATTGAGAATCTGGAGGCG
NM_001282410.1      AGTCTTCGAGAGCTGGATCTTTACGACAACCAGATCAAGAAGATTGAGAATCTGGAGGCG
NM_001282413.1      AGTCTTCGAGAGCTGGATCTTTACGACAACCAGATCAAGAAGATTGAGAATCTGGAGGCG
                    ************************************************************

HsCD00022452        CTAACAGAGCTGGAGATTCTAGATATTTCTTTTAATCTGCTGAGAAACATCGAAGGGGTT
NM_002712.2         CTAACAGAGCTGGAGATTCTAGATATTTCTTTTAATCTGCTGAGAAACATCGAAGGGGTT
NM_001282412.1      CTAACAGAGCTGGAGATTCTAGATATTTCTTTTAATCTGCTGAGAAACATCGAAGGGGTT
NM_001282410.1      CTAACAGAGCTGGAGATTCTAGATATTTCTTTTAATCTGCTGAGAAACATCGAAGGGGTT
NM_001282413.1      CTAACAGAGCTGGAGATTCTAGATATTTCTTTTAATCTGCTGAGAAACATCGAAGGGGTT
                    ************************************************************

HsCD00022452        GACAAGTTGACACGACTGAAAAAACTCTTCTTGGTCAACAATAAAATCAGTAAAATTGAG
NM_002712.2         GACAAGTTGACACGACTGAAAAAACTCTTCTTGGTCAACAATAAAATCAGTAAAATTGAG
NM_001282412.1      GACAAGTTGACACGACTGAAAAAACTCTTCTTGGTCAACAATAAAATCAGTAAAATTGAG
NM_001282410.1      GACAAGTTGACACGACTGAAAAAACTCTTCTTGGTCAACAATAAAATCAGTAAAATTGAG
NM_001282413.1      GACAAGTTGACACGACTGAAAAAACTCTTCTTGGTCAACAATAAAATCAGTAAAATTGAG
                    ************************************************************

HsCD00022452        AACTTAAGCAACTTACATCAACTACAGATGCTAGAGCTGGGATCTAACCGCATCCGGGCA
NM_002712.2         AACTTAAGCAACTTACATCAACTACAGATGCTAGAGCTGGGATCTAACCGCATCCGGGCA
NM_001282412.1      AACTTAAGCAACTTACATCAACTACAGATGCTAGAGCTGGGATCTAACCGCATCCGGGCA
NM_001282410.1      AACTTAAGCAACTTACATCAACTACAGATGCTAGAGCTGGGATCTAACCGCATCCGGGCA
NM_001282413.1      AACTTAAGCAACTTACATCAACTACAGATGCTAGAGCTGGGATCTAACCGCATCCGGGCA
                    ************************************************************

HsCD00022452        ATCGAAAATATCGACACCTTAACCAACCTGGAGAGTTTGTTTTTGGGGAAAAACAAAATT
NM_002712.2         ATCGAAAATATCGACACCTTAACCAACCTGGAGAGTTTGTTTTTGGGGAAAAACAAAATT
NM_001282412.1      ATCGAAAATATCGACACCTTAACCAACCTGGAGAGTTTGTTTTTGGGGAAAAACAAAATT
NM_001282410.1      ATCGAAAATATCGACACCTTAACCAACCTGGAGAGTTTGTTTTTGGGGAAAAACAAAATT
NM_001282413.1      ATCGAAAATATCGACACCTTAACCAACCTGGAGAGTTTGTTTTTGGGGAAAAACAAAATT
                    ************************************************************

HsCD00022452        ACTAAACTTCAGAACCTGGATGCGCTCACCAACCTGACAGTCCTCAGTATGCAGAGCAAC
NM_002712.2         ACTAAACTTCAGAACCTGGATGCGCTCACCAACCTGACAGTCCTCAGTATGCAGAGCAAC
NM_001282412.1      ACTAAACTTCAGAACCTGGATGCGCTCACCAACCTGACAGTCCTCAGTATGCAGAGCAAC
NM_001282410.1      ACTAAACTTCAGAACCTGGATGCGCTCACCAACCTGACAGTCCTCAGTATGCAGAGCAAC
NM_001282413.1      ACTAAACTTCAGAACCTGGATGCGCTCACCAACCTGACAGTCCTCAGTATGCAGAGCAAC
                    ************************************************************

HsCD00022452        CGGCTGACCAAGATCGAGGGTCTGCAGAACCTGGTGAACCTGCGGGAGCTGTACCTTAGC
NM_002712.2         CGGCTGACCAAGATCGAGGGTCTGCAGAACCTGGTGAACCTGCGGGAGCTGTACCTTAGC
NM_001282412.1      CGGCTGACCAAGATCGAGGGTCTGCAGAACCTGGTGAACCTGCGGGAGCTGTACCTTAGC
NM_001282410.1      CGGCTGACCAAGATCGAGGGTCTGCAGAACCTGGTGAACCTGCGGGAGCTGTACCTTAGC
NM_001282413.1      CGGCTGACCAAGATCGAGGGTCTGCAGAACCTGGTGAACCTGCGGGAGCTGTACCTTAGC
                    ************************************************************

HsCD00022452        CACAATGGCATCGAGGTCATCGAGGGCCTGGAGAACAATGTGCAGGACAGCCTCACGTAC
NM_002712.2         CACAATGGCATCGAGGTCATCGAGGGCCTGGAGAACAATAACAAACTCACGATGTTGGAC
NM_001282412.1      CACAATGGCATCGAGGTCATCGAGGGCCTGGAGAACAATAACAAACTCACGATGTTGGAC
NM_001282410.1      CACAATGGCATCGAGGTCATCGAGGGCCTGGAGAACAATGTGCAGGACAGCCTCACGTAC
NM_001282413.1      CACAATGGCATCGAGGTCATCGAGGGCCTGGAGAACAATAACAAACTCACGATGTTGGAC
                    ***************************************.: .*. :**  .* : * **

HsCD00022452        TAG---------------------------------------------------------
NM_002712.2         ATTGCATCAAATAGAATCAAAAAGATTGAAAATATCAGCCATCTAACAGAGCTGCAAGAG
NM_001282412.1      ATTGCATCAAATAGAATCAAAAAGATTGAAAATATCAGCCATCTAACAGAGCTGCAAGAG
NM_001282410.1      TGA---------------------------------------------------------
NM_001282413.1      ATTGCATCAAATAGAATCAAAAAGATTGAAAATATCAGCCATCTAACAGAGCTGCAAGAG
                    :                                                           

HsCD00022452        ------------------------------------------------------------
NM_002712.2         TTCTGGATGAACGACAATCTCCTTGAGAGCTGGAGCGACCTCGACGAGCTGAAGGGAGCC
NM_001282412.1      TTCTGGATGAACGACAATCTCCTTGAGAGCTGGAGCGACCTCGACGAGCTGAAGGGAGCC
NM_001282410.1      ------------------------------------------------------------
NM_001282413.1      TTCTGGATGAACGACAATCTCCTTGAGAGCTGGAGCGACCTCGACGAGCTGAAGGGAGCC
                                                                                

HsCD00022452        ------------------------------------------------------------
NM_002712.2         AGGAGCCTGGAGACAGTGTACCTGGAGCGGAACCCCTTGCAGAAGGACCCCCAGTACCGG
NM_001282412.1      AGGAGCCTGGAGACAGTGTACCTGGAGCGGAACCCCTTGCAGAAGGACCCCCAGTACCGG
NM_001282410.1      ------------------------------------------------------------
NM_001282413.1      AGGAGCCTGGAGACAGTGTACCTGGAGCGGAACCCCTTGCAGAAGGACCCCCAGTACCGG
                                                                                

HsCD00022452        ------------------------------------------------------------
NM_002712.2         CGGAAGGTCATGCTCGCCCTCCCCTCCGTGCGGCAGATCGATGCCACGTTCGTCAGGTTC
NM_001282412.1      CGGAAGGTCATGCTCGCCCTCCCCTCCGTGCGGCAGATCGATGCCACGTTCGTCAGGTTC
NM_001282410.1      ------------------------------------------------------------
NM_001282413.1      CGGAAGGTCATGCTCGCCCTCCCCTCCGTGCGGCAGATCGATGCCACGTTCGTCAGGTTC
                                                                                

HsCD00022452        ---
NM_002712.2         TGA
NM_001282412.1      TGA
NM_001282410.1      ---
NM_001282413.1      TGA
                       

Pathways

WikiPathway : Epithelium TarBase
WikiPathway : Lymphocyte TarBase
WikiPathway : Muscle cell TarBase
WikiPathway : Squamous cell TarBase

Protein

SMART domain : LRR_SD22 : Leucine-rich repeat, SDS22-like subfamily
UniProt : Q15435
HPRD : 04192

Clone

Cloning Information : 24632
HIP Master Clone ID : 55008
Original Clone ID : FLH024632.01L

Organism


TAX_ID : 9606
Species Specific ID: 5510

Genome

Chromosome : 2
Map Location : 2q37.3
Ensembl : ENSG00000115685

Reagents

Labome : PPP1R7-antibody