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CHRNA1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CHRNA1
Gene Name:  cholinergic receptor, nicotinic, alpha 1 (muscle)
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH025532.01L
Keyword: potential short variant
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: Short variant or partial clone comparing to NM_000079
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00022478

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 810nts         Open reading frame : 1 to 810
Reference Sequence Alignment

ATGGAGCCCTGGCCTCTCCTCCTGCTCTTTAGCCTTTGCTCAGCTGGCCTCGTCCTGGGC
TCCGAACATGAGACCCGTCTGGTGGCAAAGCTATTTAAAGACTACAGCAGCGTGGTGCGG
CCAGTGGAAGACCACCGCCAGGTCGTGGAGGTCACCGTGGGCCTGCAGCTGATACAGCTC
ATCAATGTGGATGAAGTAAATCAGATCGTGACAACCAATGTGCGTCTGAAACAGCAATGG
GTGGATTACAACCTAAAATGGAATCCAGATGACTATGGCGGTGTGAAAAAAATTCACATT
CCTTCAGAAAAGATCTGGCGCCCAGACCTTGTTCTCTATAACAATGCAGATGGTGACTTT
GCTATTGTCAAGTTCACCAAAGTGCTCCTGCAGTACACTGGCCACATCACGTGGACACCT
CCAGCCATCTTTAAAAGCTACTGTGAGATCATCGTCACCCACTTTCCCTTTGATGAACAG
AACTGCAGCATGAAGCTGGGCACCTGGACCTACGACGGCTCTGTCGTGGCCATCAACCCG
GAAAGCGACCAGCCAGACCTGAGCAACTTCATGGAGAGCGGGGAGTGGGTGATCAAGGAG
TCCCGGGGCTGGAAGCACTCCGTGACCTATTCCTGCTGCCCCGACACCCCCTACCTGGAC
ATCACCTACCACTTCGTCATGCAGCGCCTGCCCCTCTACTTCATCGTCAACGTCATCATC
CCCTGCCTGCTCTTCTCCTTCTTAACTGGCCTGGTATTCTACCTGCCCACAGACTCAGGT
GGGTGTGGTTGCCATGACTGCTGCTGCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 878
Start on reference sequence 69
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CHRNA1
Symbol Nomenclature : CHRNA1
SYNONYM : ACHRA; ACHRD; CHRNA; CMS2A; FCCMS; SCCMS
Designation : acetylcholine receptor subunit alpha|acetylcholine receptor, nicotinic, alpha 1 (muscle)|cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle)|muscle nicotinic acetylcholine receptor|nicotinic acetylcholine receptor alpha subunit|nicotinic cholinergic receptor alpha 1
Full Nomenclature : cholinergic receptor, nicotinic, alpha 1 (muscle)
GENEID : 1134
HGNC : 1955
MIM : 100690
Vega : OTTHUMG00000132357
Target GenBank: BC006314

 

Reference Sequence Alignment

  

HsCD00022478        ATGGAGCCCTGGCCTCTCCTCCTGCTCTTTAGCCTTTGCTCAGCTGGCCTCGTCCTGGGC
NM_000079.3         ATGGAGCCCTGGCCTCTCCTCCTGCTCTTTAGCCTTTGCTCAGCTGGCCTCGTCCTGGGC
NM_001039523.2      ATGGAGCCCTGGCCTCTCCTCCTGCTCTTTAGCCTTTGCTCAGCTGGCCTCGTCCTGGGC
                    ************************************************************

HsCD00022478        TCCGAACATGAGACCCGTCTGGTGGCAAAGCTATTTAAAGACTACAGCAGCGTGGTGCGG
NM_000079.3         TCCGAACATGAGACCCGTCTGGTGGCAAAGCTATTTAAAGACTACAGCAGCGTGGTGCGG
NM_001039523.2      TCCGAACATGAGACCCGTCTGGTGGCAAAGCTATTTAAAGACTACAGCAGCGTGGTGCGG
                    ************************************************************

HsCD00022478        CCAGTGGAAGACCACCGCCAGGTCGTGGAGGTCACCGTGGGCCTGCAGCTGATACAGCTC
NM_000079.3         CCAGTGGAAGACCACCGCCAGGTCGTGGAGGTCACCGTGGGCCTGCAGCTGATACAGCTC
NM_001039523.2      CCAGTGGAAGACCACCGCCAGGTCGTGGAGGTCACCGTGGGCCTGCAGCTGATACAGCTC
                    ************************************************************

HsCD00022478        ATCAATGTGGATGAAGTAAATCAGATCGTGACAACCAATGTGCGTCTGAAACAG------
NM_000079.3         ATCAATGTGGATGAAGTAAATCAGATCGTGACAACCAATGTGCGTCTGAAACAG------
NM_001039523.2      ATCAATGTGGATGAAGTAAATCAGATCGTGACAACCAATGTGCGTCTGAAACAGGGTGAC
                    ******************************************************      

HsCD00022478        ------------------------------------------------------------
NM_000079.3         ------------------------------------------------------------
NM_001039523.2      ATGGTAGATCTGCCACGCCCCAGCTGCGTGACTTTGGGAGTTCCTTTGTTTTCTCATCTG
                                                                                

HsCD00022478        ---------CAATGGGTGGATTACAACCTAAAATGGAATCCAGATGACTATGGCGGTGTG
NM_000079.3         ---------CAATGGGTGGATTACAACCTAAAATGGAATCCAGATGACTATGGCGGTGTG
NM_001039523.2      CAGAATGAGCAATGGGTGGATTACAACCTAAAATGGAATCCAGATGACTATGGCGGTGTG
                             ***************************************************

HsCD00022478        AAAAAAATTCACATTCCTTCAGAAAAGATCTGGCGCCCAGACCTTGTTCTCTATAACAAT
NM_000079.3         AAAAAAATTCACATTCCTTCAGAAAAGATCTGGCGCCCAGACCTTGTTCTCTATAACAAT
NM_001039523.2      AAAAAAATTCACATTCCTTCAGAAAAGATCTGGCGCCCAGACCTTGTTCTCTATAACAAT
                    ************************************************************

HsCD00022478        GCAGATGGTGACTTTGCTATTGTCAAGTTCACCAAAGTGCTCCTGCAGTACACTGGCCAC
NM_000079.3         GCAGATGGTGACTTTGCTATTGTCAAGTTCACCAAAGTGCTCCTGCAGTACACTGGCCAC
NM_001039523.2      GCAGATGGTGACTTTGCTATTGTCAAGTTCACCAAAGTGCTCCTGCAGTACACTGGCCAC
                    ************************************************************

HsCD00022478        ATCACGTGGACACCTCCAGCCATCTTTAAAAGCTACTGTGAGATCATCGTCACCCACTTT
NM_000079.3         ATCACGTGGACACCTCCAGCCATCTTTAAAAGCTACTGTGAGATCATCGTCACCCACTTT
NM_001039523.2      ATCACGTGGACACCTCCAGCCATCTTTAAAAGCTACTGTGAGATCATCGTCACCCACTTT
                    ************************************************************

HsCD00022478        CCCTTTGATGAACAGAACTGCAGCATGAAGCTGGGCACCTGGACCTACGACGGCTCTGTC
NM_000079.3         CCCTTTGATGAACAGAACTGCAGCATGAAGCTGGGCACCTGGACCTACGACGGCTCTGTC
NM_001039523.2      CCCTTTGATGAACAGAACTGCAGCATGAAGCTGGGCACCTGGACCTACGACGGCTCTGTC
                    ************************************************************

HsCD00022478        GTGGCCATCAACCCGGAAAGCGACCAGCCAGACCTGAGCAACTTCATGGAGAGCGGGGAG
NM_000079.3         GTGGCCATCAACCCGGAAAGCGACCAGCCAGACCTGAGCAACTTCATGGAGAGCGGGGAG
NM_001039523.2      GTGGCCATCAACCCGGAAAGCGACCAGCCAGACCTGAGCAACTTCATGGAGAGCGGGGAG
                    ************************************************************

HsCD00022478        TGGGTGATCAAGGAGTCCCGGGGCTGGAAGCACTCCGTGACCTATTCCTGCTGCCCCGAC
NM_000079.3         TGGGTGATCAAGGAGTCCCGGGGCTGGAAGCACTCCGTGACCTATTCCTGCTGCCCCGAC
NM_001039523.2      TGGGTGATCAAGGAGTCCCGGGGCTGGAAGCACTCCGTGACCTATTCCTGCTGCCCCGAC
                    ************************************************************

HsCD00022478        ACCCCCTACCTGGACATCACCTACCACTTCGTCATGCAGCGCCTGCCCCTCTACTTCATC
NM_000079.3         ACCCCCTACCTGGACATCACCTACCACTTCGTCATGCAGCGCCTGCCCCTCTACTTCATC
NM_001039523.2      ACCCCCTACCTGGACATCACCTACCACTTCGTCATGCAGCGCCTGCCCCTCTACTTCATC
                    ************************************************************

HsCD00022478        GTCAACGTCATCATCCCCTGCCTGCTCTTCTCCTTCTTAACTGGCCTGGTATTCTACCTG
NM_000079.3         GTCAACGTCATCATCCCCTGCCTGCTCTTCTCCTTCTTAACTGGCCTGGTATTCTACCTG
NM_001039523.2      GTCAACGTCATCATCCCCTGCCTGCTCTTCTCCTTCTTAACTGGCCTGGTATTCTACCTG
                    ************************************************************

HsCD00022478        CCCACAGACTCAGGTGGGTGTGGTTGCCATGACTGCTGCTGCTTG---------------
NM_000079.3         CCCACAGACTCAGGGGAGAAGATGACTCTGAGCATCTCTGTCTTACTGTCTTTGACTGTG
NM_001039523.2      CCCACAGACTCAGGGGAGAAGATGACTCTGAGCATCTCTGTCTTACTGTCTTTGACTGTG
                    ************** *.*:. .  :  *: ..*: **    ***.               

HsCD00022478        ------------------------------------------------------------
NM_000079.3         TTCCTTCTGGTCATCGTGGAGCTGATCCCCTCCACGTCCAGTGCTGTGCCCTTGATTGGA
NM_001039523.2      TTCCTTCTGGTCATCGTGGAGCTGATCCCCTCCACGTCCAGTGCTGTGCCCTTGATTGGA
                                                                                

HsCD00022478        ------------------------------------------------------------
NM_000079.3         AAATACATGCTGTTCACCATGGTGTTCGTCATTGCCTCCATCATCATCACTGTCATCGTC
NM_001039523.2      AAATACATGCTGTTCACCATGGTGTTCGTCATTGCCTCCATCATCATCACTGTCATCGTC
                                                                                

HsCD00022478        ------------------------------------------------------------
NM_000079.3         ATCAACACACACCACCGCTCACCCAGCACCCATGTCATGCCCAACTGGGTGCGGAAGGTT
NM_001039523.2      ATCAACACACACCACCGCTCACCCAGCACCCATGTCATGCCCAACTGGGTGCGGAAGGTT
                                                                                

HsCD00022478        ------------------------------------------------------------
NM_000079.3         TTTATCGACACTATCCCAAATATCATGTTTTTCTCCACAATGAAAAGACCATCCAGAGAA
NM_001039523.2      TTTATCGACACTATCCCAAATATCATGTTTTTCTCCACAATGAAAAGACCATCCAGAGAA
                                                                                

HsCD00022478        ------------------------------------------------------------
NM_000079.3         AAGCAAGACAAAAAGATTTTTACAGAAGACATTGATATCTCTGACATTTCTGGAAAGCCA
NM_001039523.2      AAGCAAGACAAAAAGATTTTTACAGAAGACATTGATATCTCTGACATTTCTGGAAAGCCA
                                                                                

HsCD00022478        ------------------------------------------------------------
NM_000079.3         GGGCCTCCACCCATGGGCTTCCACTCTCCCCTGATCAAACACCCCGAGGTGAAAAGTGCC
NM_001039523.2      GGGCCTCCACCCATGGGCTTCCACTCTCCCCTGATCAAACACCCCGAGGTGAAAAGTGCC
                                                                                

HsCD00022478        ------------------------------------------------------------
NM_000079.3         ATCGAGGGCATCAAGTACATCGCAGAGACCATGAAGTCAGACCAGGAGTCTAACAATGCG
NM_001039523.2      ATCGAGGGCATCAAGTACATCGCAGAGACCATGAAGTCAGACCAGGAGTCTAACAATGCG
                                                                                

HsCD00022478        ------------------------------------------------------------
NM_000079.3         GCGGCAGAGTGGAAGTACGTTGCAATGGTGATGGACCACATACTCCTCGGAGTCTTCATG
NM_001039523.2      GCGGCAGAGTGGAAGTACGTTGCAATGGTGATGGACCACATACTCCTCGGAGTCTTCATG
                                                                                

HsCD00022478        ------------------------------------------------------------
NM_000079.3         CTTGTTTGCATCATCGGAACCCTAGCCGTGTTTGCAGGTCGACTCATTGAATTAAATCAG
NM_001039523.2      CTTGTTTGCATCATCGGAACCCTAGCCGTGTTTGCAGGTCGACTCATTGAATTAAATCAG
                                                                                

HsCD00022478        ---------
NM_000079.3         CAAGGATGA
NM_001039523.2      CAAGGATGA
                             

Pathways

NCI : Effects of Botulinum toxin
NCI : ErbB2/ErbB3 signaling events
Panther : Nicotinic acetylcholine receptor signaling pathway
Reactome : Acetylcholine Binding And Downstream Events
Reactome : Activation of Nicotinic Acetylcholine Receptors
Reactome : Highly calcium permeable nicotinic acetylcholine receptors
Reactome : Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
Reactome : Neuronal System
Reactome : Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome : Postsynaptic nicotinic acetylcholine receptors
Reactome : Presynaptic nicotinic acetylcholine receptors
Reactome : Transmission across Chemical Synapses

Protein

UniProt : P02708
UniProt : Q53SH4
HPRD : 00007

Clone

Cloning Information : 25532
HIP Master Clone ID : 55908
Original Clone ID : FLH025532.01L

Organism


TAX_ID : 9606
Species Specific ID: 1134

Genome

Chromosome : 2
Map Location : 2q31.1
Ensembl : ENSG00000138435

Reagents

Labome : CHRNA1-antibody